##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936160_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2762533 Sequences flagged as poor quality 0 Sequence length 77 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.263290972451735 40.0 37.0 41.0 31.0 41.0 2 37.13644108504767 40.0 37.0 41.0 31.0 41.0 3 37.00575848324708 40.0 36.0 41.0 31.0 41.0 4 36.906079673980365 39.0 36.0 41.0 31.0 41.0 5 36.82700116161508 39.0 36.0 41.0 30.0 41.0 6 36.735048594894614 39.0 36.0 41.0 30.0 41.0 7 36.58319773917633 39.0 36.0 41.0 30.0 41.0 8 36.46116010197887 39.0 36.0 40.0 30.0 41.0 9 36.61133170173895 39.0 36.0 41.0 30.0 41.0 10-11 36.84362286350968 39.5 36.0 41.0 30.5 41.0 12-13 36.7653977346153 40.0 36.0 41.0 30.0 41.0 14-15 36.60631547206857 40.0 36.0 41.0 30.0 41.0 16-17 36.44661584133112 40.0 36.0 41.0 30.0 41.0 18-19 36.28473813706479 39.0 35.0 41.0 30.0 41.0 20-21 36.08516875635513 39.0 35.0 41.0 29.0 41.0 22-23 35.815753332177394 39.0 35.0 41.0 28.0 41.0 24-25 35.57829644025972 39.0 35.0 41.0 27.5 41.0 26-27 34.59327979068485 38.0 34.0 40.0 25.5 40.5 28-29 34.59085773817 38.0 34.0 39.5 26.0 40.5 30-31 34.98347368158136 38.0 34.0 40.0 26.0 41.0 32-33 34.834310757554746 38.0 34.0 40.0 26.0 41.0 34-35 34.56810235389044 38.0 34.0 40.0 26.0 41.0 36-37 34.24670836511274 37.5 34.0 40.0 25.0 41.0 38-39 33.925207952266994 37.0 33.5 40.0 24.0 41.0 40-41 33.555567119017226 36.5 33.0 39.5 23.0 41.0 42-43 33.17985938991498 36.0 33.0 39.0 22.0 41.0 44-45 32.80913476870684 35.5 32.5 39.0 22.0 40.5 46-47 32.37818733749063 35.0 32.0 38.5 20.5 40.0 48-49 31.97145246771713 35.0 32.0 37.5 19.5 40.0 50-51 31.53133682022984 35.0 31.0 37.0 18.0 39.0 52-53 31.070129298002954 35.0 31.0 36.0 14.0 39.0 54-55 30.618130715542584 35.0 31.0 36.0 9.0 38.5 56-57 30.200273263704 34.0 30.0 35.5 7.0 37.0 58-59 29.784442393991313 34.0 30.0 35.0 2.0 37.0 60-61 29.440641795048244 34.0 29.5 35.0 2.0 36.0 62-63 29.092868211891044 34.0 29.0 35.0 2.0 36.0 64-65 28.70973324119567 34.0 29.0 35.0 2.0 35.5 66-67 28.36573499755478 34.0 29.0 35.0 2.0 35.0 68-69 28.013499386251674 34.0 28.5 35.0 2.0 35.0 70-71 27.562059892135224 34.0 27.0 35.0 2.0 35.0 72-73 27.080753967463917 33.0 26.5 35.0 2.0 35.0 74-75 26.501442878691407 33.0 25.0 35.0 2.0 35.0 76-77 24.666887961157386 31.0 20.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.8386987824198968 1101 2 0.7539989020035947 1101 3 0.8811180655198712 1101 4 0.7722398842841756 1101 5 0.836365910555422 1101 6 0.8176004096124174 1101 7 0.8957472322342497 1101 8 0.8261957165409228 1101 9 0.8822907890762082 1101 10-11 0.8068976557494665 1101 12-13 0.976219048433137 1101 14-15 1.0028054706538896 1101 16-17 0.92896893089965 1101 18-19 1.0300057571082775 1101 20-21 0.9407443391582433 1101 22-23 0.9353722180844528 1101 24-25 0.978394099249634 1101 26-27 1.0910579329428103 1101 28-29 1.1500255542711955 1101 30-31 1.1060936771358314 1101 32-33 1.091112040952737 1101 34-35 1.1230640913711198 1101 36-37 1.2153115128718923 1101 38-39 1.142352620281116 1101 40-41 1.1186546657301122 1101 42-43 1.1010176434415229 1101 44-45 1.083967088347407 1101 46-47 1.0611779995342445 1101 48-49 1.092482692704852 1101 50-51 1.0645722022767536 1101 52-53 0.99210984085083 1101 54-55 1.0895864355887888 1101 56-57 1.0973994339802644 1101 58-59 1.1413527645515877 1101 60-61 1.0975881297952945 1101 62-63 1.1592104520358184 1101 64-65 1.078109831048284 1101 66-67 1.0751284612985117 1101 68-69 1.0105807866382222 1101 70-71 1.2098021998385775 1101 72-73 1.0586187840892798 1101 74-75 1.0907376378211353 1101 76-77 1.2941575103883274 1102 1 0.7311692721331866 1102 2 0.745396938999292 1102 3 0.7568610026176827 1102 4 0.7299478724389843 1102 5 0.5846950362841241 1102 6 0.7339378549393842 1102 7 0.5816821096131406 1102 8 0.6765667616868569 1102 9 0.7242329799758167 1102 10-11 0.748488079851505 1102 12-13 0.6697272538026837 1102 14-15 0.7056983039368916 1102 16-17 0.6773187403142558 1102 18-19 0.6920621478987741 1102 20-21 0.6431193596077023 1102 22-23 0.736395880365265 1102 24-25 0.6679328386402403 1102 26-27 0.4886477430204508 1102 28-29 0.6326188272446771 1102 30-31 0.8702876944337419 1102 32-33 0.8133423188420181 1102 34-35 0.7083079030212929 1102 36-37 0.7733337200878339 1102 38-39 0.7818574513991905 1102 40-41 0.7849311049939516 1102 42-43 0.6899528804426893 1102 44-45 0.50443507295644 1102 46-47 0.6139813596602721 1102 48-49 0.5229562482160439 1102 50-51 0.6416555431172277 1102 52-53 0.6941836145857216 1102 54-55 0.7001150803317095 1102 56-57 0.6221373385282973 1102 58-59 0.634957397697228 1102 60-61 0.46006583357464237 1102 62-63 0.43757358693308746 1102 64-65 0.4604672235201406 1102 66-67 0.5849302942910839 1102 68-69 0.6386218830321262 1102 70-71 0.6341442316107404 1102 72-73 0.31539197053721324 1102 74-75 0.30830197885835986 1102 76-77 0.3558827459985707 1103 1 0.3786756159060758 1103 2 0.4207365508228307 1103 3 0.25287521153359904 1103 4 0.39772860212897854 1103 5 0.3122148055808296 1103 6 0.42612167391904165 1103 7 0.40102309521121526 1103 8 0.3169561586452474 1103 9 0.3310080442650616 1103 10-11 0.3665784764863318 1103 12-13 0.3633675150469102 1103 14-15 0.3572061208275059 1103 16-17 0.29472608149286117 1103 18-19 0.1956265818979901 1103 20-21 0.335298991981098 1103 22-23 0.2993500207059867 1103 24-25 0.3394374481646949 1103 26-27 0.34791923095450983 1103 28-29 0.3546448466478225 1103 30-31 0.3620317778366129 1103 32-33 0.29056881282182445 1103 34-35 0.47459912335315835 1103 36-37 0.5662144316122095 1103 38-39 0.37290308114992143 1103 40-41 0.4473358774412546 1103 42-43 0.34341768740466705 1103 44-45 0.39624784508584554 1103 46-47 0.4166970479383494 1103 48-49 0.4189945123781236 1103 50-51 0.40334511548732976 1103 52-53 0.6516851615012555 1103 54-55 0.5035363891641715 1103 56-57 0.4822308914384834 1103 58-59 0.4669474690540305 1103 60-61 0.33036172019850696 1103 62-63 0.21542445292195112 1103 64-65 0.04808402273902246 1103 66-67 0.06936917551635347 1103 68-69 0.15411779009230742 1103 70-71 0.09119674726439086 1103 72-73 0.081410351867504 1103 74-75 0.1946017001297271 1103 76-77 0.2445788381209404 1104 1 0.44651742144218076 1104 2 0.3365568863322892 1104 3 0.48156328154291117 1104 4 0.3994162631430598 1104 5 0.48563433910398857 1104 6 0.6114112380946182 1104 7 0.5095774304494896 1104 8 0.4815231127199766 1104 9 0.3461228074368208 1104 10-11 0.47934612940941435 1104 12-13 0.498029610525073 1104 14-15 0.6242217949027662 1104 16-17 0.6100794622607495 1104 18-19 0.5229233457232496 1104 20-21 0.42306000428875024 1104 22-23 0.43426023625092114 1104 24-25 0.39079177251124975 1104 26-27 0.2580029360511702 1104 28-29 0.36741969588640444 1104 30-31 0.5074152719895366 1104 32-33 0.5185585068089296 1104 34-35 0.5794031779629094 1104 36-37 0.5243339593560989 1104 38-39 0.5128480779602 1104 40-41 0.6439018457041072 1104 42-43 0.56855789052414 1104 44-45 0.4888420584702189 1104 46-47 0.26305751832730806 1104 48-49 0.33555351216251594 1104 50-51 0.3177928719040004 1104 52-53 0.3723303070168704 1104 54-55 0.31815471770737247 1104 56-57 0.3411853307038015 1104 58-59 0.22868968869780204 1104 60-61 0.3292015574450815 1104 62-63 0.2468201157942964 1104 64-65 0.12654178286776485 1104 66-67 0.19897009397737264 1104 68-69 -0.019097106892019156 1104 70-71 0.13609230899933067 1104 72-73 0.18771576905374943 1104 74-75 -0.005610910704646699 1104 76-77 0.025926849715062872 1105 1 0.11348799709460167 1105 2 0.33176831653349836 1105 3 0.1977600505010031 1105 4 0.31319131254581123 1105 5 -0.046574626052638735 1105 6 0.09797871444580863 1105 7 0.23460245438690208 1105 8 0.17247768935026642 1105 9 0.28548651356004484 1105 10-11 0.3418714759077659 1105 12-13 0.4472378762337854 1105 14-15 0.40651618777518905 1105 16-17 0.3584650957364701 1105 18-19 0.3487913062553716 1105 20-21 0.3304650525370718 1105 22-23 0.29040430893917346 1105 24-25 0.22943090492420737 1105 26-27 0.032354928119559645 1105 28-29 0.16254997654836956 1105 30-31 0.3488777292926528 1105 32-33 0.39912716475173227 1105 34-35 0.3145558002135047 1105 36-37 0.42778542921531937 1105 38-39 0.26565551248033614 1105 40-41 0.4006183112512076 1105 42-43 0.43616088932218133 1105 44-45 0.2827670477747759 1105 46-47 0.2385060817427913 1105 48-49 0.2601300745458026 1105 50-51 0.3232930450682865 1105 52-53 0.27405285029096405 1105 54-55 -0.014725106276220146 1105 56-57 0.024872983071404065 1105 58-59 0.18856867741835615 1105 60-61 0.15980430498512987 1105 62-63 0.09655140556266417 1105 64-65 0.2514743167027085 1105 66-67 0.1735177277383002 1105 68-69 0.057356556381531476 1105 70-71 -0.043238054509863844 1105 72-73 -0.4375807493254307 1105 74-75 -0.8606271066587148 1105 76-77 -0.8863787479054537 1106 1 0.025857040091949557 1106 2 0.14978742798061262 1106 3 0.11666076769108713 1106 4 0.14456173816032702 1106 5 0.25457587489067635 1106 6 0.19560524434916005 1106 7 -0.20319080688687308 1106 8 0.0924005897132858 1106 9 0.03718944838632865 1106 10-11 -0.0803606675574926 1106 12-13 0.2602993856703151 1106 14-15 0.06272413884553174 1106 16-17 0.205439411749623 1106 18-19 0.2840362610649052 1106 20-21 0.23855288618711512 1106 22-23 0.26459268541970005 1106 24-25 0.03619810079951691 1106 26-27 -0.006568469440765057 1106 28-29 0.07457343336371736 1106 30-31 0.2928017738905737 1106 32-33 0.20165626441595919 1106 34-35 0.19655841560754794 1106 36-37 0.2333613948337856 1106 38-39 0.38274895151917576 1106 40-41 0.30409004251946925 1106 42-43 0.3643400574361664 1106 44-45 0.22651087537671089 1106 46-47 0.24004698473482478 1106 48-49 0.03628510475223479 1106 50-51 0.13075236683667768 1106 52-53 0.2861799012298505 1106 54-55 0.2845015135313034 1106 56-57 0.19629183434129516 1106 58-59 0.11600995024345906 1106 60-61 0.05753279439074177 1106 62-63 -0.02003121432658972 1106 64-65 -0.018918809714811857 1106 66-67 -0.05660813810015952 1106 68-69 0.07198791522857562 1106 70-71 -0.11819415811760692 1106 72-73 -0.17139200505512164 1106 74-75 0.03595251642486019 1106 76-77 -0.11955325053787647 1107 1 -0.16385484068688072 1107 2 -0.20419733192355238 1107 3 -0.05619415612952139 1107 4 -0.1941605171038958 1107 5 -0.1752431779709056 1107 6 -0.32508229994117954 1107 7 -0.2874516010759791 1107 8 -0.21868317386908132 1107 9 -0.2768185323115162 1107 10-11 -0.156222378345781 1107 12-13 -0.11836972645595978 1107 14-15 -0.2850504823665929 1107 16-17 -0.21030256220765153 1107 18-19 -0.1479730546703948 1107 20-21 -0.3305316113495067 1107 22-23 -0.24357980542696112 1107 24-25 -0.24044978992566968 1107 26-27 -0.45528223045008076 1107 28-29 -0.32519514959842155 1107 30-31 -0.1627179363662279 1107 32-33 -0.024021878721995904 1107 34-35 -0.1545833261417897 1107 36-37 -0.030605221848063024 1107 38-39 -0.16330944529698144 1107 40-41 -0.09386263641351178 1107 42-43 -0.10355840800893645 1107 44-45 -0.05838345524858113 1107 46-47 -0.12734983985854598 1107 48-49 -0.05093813333577302 1107 50-51 0.06486903287062162 1107 52-53 -0.044787589658994875 1107 54-55 -0.03432053736462848 1107 56-57 0.06198216259600642 1107 58-59 -0.07694105725637357 1107 60-61 -0.019971745369328886 1107 62-63 0.041001272639395125 1107 64-65 0.024813948687697263 1107 66-67 0.08827071199601377 1107 68-69 -0.07674393100379007 1107 70-71 -0.0777552302813369 1107 72-73 -0.10149129978414706 1107 74-75 -0.14307511020333763 1107 76-77 -0.07118490147092515 1108 1 0.056937564295139964 1108 2 0.24282452863114656 1108 3 0.25443397368557896 1108 4 0.12245438723611812 1108 5 0.11943092000033317 1108 6 0.12489884091655057 1108 7 0.31888587324348805 1108 8 0.21733217025007434 1108 9 0.09306141119130729 1108 10-11 0.19454362383707036 1108 12-13 0.2464794405317221 1108 14-15 0.3462693365856424 1108 16-17 0.2142478278154556 1108 18-19 0.20490757899678158 1108 20-21 0.2232049063700643 1108 22-23 0.13283763968090057 1108 24-25 0.25183501522328555 1108 26-27 0.18575706879234133 1108 28-29 0.3158491653733293 1108 30-31 0.385325728567004 1108 32-33 0.3054804405512499 1108 34-35 0.2943966751422309 1108 36-37 0.2764707684575285 1108 38-39 0.4157133702525755 1108 40-41 0.3264093364935192 1108 42-43 0.3971758947645583 1108 44-45 0.4822931064488998 1108 46-47 0.31905809542650587 1108 48-49 0.35041852113264227 1108 50-51 0.28673380078279465 1108 52-53 0.4632817156825553 1108 54-55 0.4497765912306484 1108 56-57 0.5498541290829095 1108 58-59 0.47426251715297596 1108 60-61 0.5265812448078648 1108 62-63 0.5320281328730871 1108 64-65 0.45626497911913333 1108 66-67 0.6076304240530241 1108 68-69 0.7200979651826529 1108 70-71 0.5866222575204105 1108 72-73 0.4821951408938183 1108 74-75 0.6070151729245552 1108 76-77 0.518998914977459 1109 1 0.1326253667813333 1109 2 0.0589126749441391 1109 3 -0.14357631729671283 1109 4 0.08332547799289358 1109 5 0.10386590854605515 1109 6 -0.09299345315461949 1109 7 -0.10336797892686889 1109 8 0.22759809010587162 1109 9 0.003816569585886498 1109 10-11 -0.03583236724566774 1109 12-13 0.030184938584291388 1109 14-15 0.02237511097014533 1109 16-17 0.08576650702028843 1109 18-19 -0.07243417035109445 1109 20-21 -0.05458610569564115 1109 22-23 0.013852251327335807 1109 24-25 0.06751571089686337 1109 26-27 0.10466372140419367 1109 28-29 0.19321061726735422 1109 30-31 0.3183912908985178 1109 32-33 0.2674072552698945 1109 34-35 0.32849250197423174 1109 36-37 0.4318679013152291 1109 38-39 0.3757317179384998 1109 40-41 0.3909692947658172 1109 42-43 0.3473460711643881 1109 44-45 0.3820127297878386 1109 46-47 0.35804233735988333 1109 48-49 0.3162715768635529 1109 50-51 0.3420262421515936 1109 52-53 0.30666111426357645 1109 54-55 0.43758942498284625 1109 56-57 0.42030529753818513 1109 58-59 0.5257964621089251 1109 60-61 0.5021677057342906 1109 62-63 0.4260733484794912 1109 64-65 0.5779384994926531 1109 66-67 0.5178385514277544 1109 68-69 0.24323778675798025 1109 70-71 0.5120091619278213 1109 72-73 0.31837430105914066 1109 74-75 0.37896614187294375 1109 76-77 0.4086116780585556 1110 1 -0.3986000066136981 1110 2 -0.30523866141891176 1110 3 -0.5428644497960988 1110 4 -0.36924309999023563 1110 5 -0.36450718599365217 1110 6 -0.46116451868250863 1110 7 -0.24551688322092957 1110 8 -0.6377914424655273 1110 9 -0.477808274597038 1110 10-11 -0.45124570578342826 1110 12-13 -0.4271793263010508 1110 14-15 -0.28569905545762 1110 16-17 -0.444969998106572 1110 18-19 -0.5212540831006791 1110 20-21 -0.6085033429071913 1110 22-23 -0.5698580098067225 1110 24-25 -0.5836058265443924 1110 26-27 -0.6683248233744905 1110 28-29 -0.35343168104976286 1110 30-31 -0.40706062331326365 1110 32-33 -0.37187230326883025 1110 34-35 -0.3561556522770033 1110 36-37 -0.41189942741466723 1110 38-39 -0.43627448400424385 1110 40-41 -0.3789071382091933 1110 42-43 -0.35895049970411463 1110 44-45 -0.38565390661499066 1110 46-47 -0.39976787833075633 1110 48-49 -0.43762870544804144 1110 50-51 -0.5549343745825972 1110 52-53 -0.5474081332176723 1110 54-55 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9 -0.315918105231475 2314 10-11 -0.46261444626875914 2314 12-13 -0.43882349674944265 2314 14-15 -0.41397099982366825 2314 16-17 -0.37977596721829343 2314 18-19 -0.5210157168385834 2314 20-21 -0.5313634827365732 2314 22-23 -0.3665650045692104 2314 24-25 -0.2677661846044188 2314 26-27 -0.26919521424045456 2314 28-29 -0.365086847241578 2314 30-31 -0.4756018657721981 2314 32-33 -0.6711480646060579 2314 34-35 -0.7341186698908899 2314 36-37 -1.2512528498102995 2314 38-39 -0.8259863729989476 2314 40-41 -0.545677164863946 2314 42-43 -0.4013588829018033 2314 44-45 -0.37553728629672634 2314 46-47 -0.27045310838691705 2314 48-49 -0.3715940633852348 2314 50-51 -0.23121502199909472 2314 52-53 -0.19543149010764438 2314 54-55 -0.26284279055334636 2314 56-57 -0.36555409696656227 2314 58-59 -0.32275052536557425 2314 60-61 -0.31565282883328294 2314 62-63 -0.3190828873826064 2314 64-65 -0.3068173631454414 2314 66-67 -0.16850694799722632 2314 68-69 -0.1163100048332737 2314 70-71 0.0010643814281330322 2314 72-73 0.1379206742746959 2314 74-75 0.08869905594805516 2314 76-77 -0.019773243318297773 2315 1 -0.9536024354086763 2315 2 -0.8089656284206299 2315 3 -0.6861717974675443 2315 4 -0.6097444672707013 2315 5 -0.8556571948599796 2315 6 -0.8169360955148761 2315 7 -1.3125404027559497 2315 8 -1.3638394797191964 2315 9 -0.9305292138318322 2315 10-11 -1.0640459552848895 2315 12-13 -1.0348656624293895 2315 14-15 -1.0608560271658334 2315 16-17 -0.8969608544601257 2315 18-19 -0.9743476190395128 2315 20-21 -0.8901050654183678 2315 22-23 -1.0638748522665935 2315 24-25 -1.1449072340010886 2315 26-27 -0.9974288202421633 2315 28-29 -0.9944028412760062 2315 30-31 -1.0861681422669776 2315 32-33 -1.0676094014343676 2315 34-35 -1.1826656938887226 2315 36-37 -1.1035317398541409 2315 38-39 -1.1091020235616043 2315 40-41 -1.2382021343582252 2315 42-43 -1.1412637502584246 2315 44-45 -1.0639891776511767 2315 46-47 -0.8941085971562934 2315 48-49 -0.8914623238814663 2315 50-51 -0.8358157360588407 2315 52-53 -0.8962551444665721 2315 54-55 -1.07013139723297 2315 56-57 -1.1399304245301707 2315 58-59 -1.0058808638660466 2315 60-61 -1.125071186495866 2315 62-63 -1.0318037713704946 2315 64-65 -0.9478441629827401 2315 66-67 -0.9106827965080981 2315 68-69 -1.0218439678648643 2315 70-71 -1.0257806495295227 2315 72-73 -0.9178821910030202 2315 74-75 -0.9254239611803392 2315 76-77 -0.94211924593953 2316 1 -1.5425171795481702 2316 2 -1.1922031983798433 2316 3 -1.3891769218405443 2316 4 -2.0174288004730343 2316 5 -1.7277690482878114 2316 6 -1.5836086989944889 2316 7 -1.4695960468259486 2316 8 -1.1092368364177645 2316 9 -1.3834931999629205 2316 10-11 -1.3193936847443268 2316 12-13 -2.0106635507308326 2316 14-15 -3.224141936979187 2316 16-17 -2.3911390672600206 2316 18-19 -1.7676337611629478 2316 20-21 -1.4903275023123044 2316 22-23 -1.7407533211883433 2316 24-25 -1.8667021293744384 2316 26-27 -1.6890275200801312 2316 28-29 -2.2028089328332783 2316 30-31 -2.3861864366851506 2316 32-33 -2.4548809389979453 2316 34-35 -2.378069923093584 2316 36-37 -2.415691865379017 2316 38-39 -2.61330949775099 2316 40-41 -2.1362533432417656 2316 42-43 -2.4902965659920113 2316 44-45 -2.4432886079107234 2316 46-47 -2.372561837978594 2316 48-49 -2.0971286085271252 2316 50-51 -2.275988390840265 2316 52-53 -2.3045744585323646 2316 54-55 -2.2291140049522475 2316 56-57 -2.1034666968440376 2316 58-59 -2.170481503493111 2316 60-61 -1.9284030453055472 2316 62-63 -2.116160640295341 2316 64-65 -2.12750986878914 2316 66-67 -2.233952425257243 2316 68-69 -2.2037028259447062 2316 70-71 -2.1117249706130146 2316 72-73 -2.135233914259775 2316 74-75 -2.3248424116334405 2316 76-77 -2.623328119797094 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 44693.0 3 4888.0 4 6384.0 5 7888.0 6 8991.0 7 9778.0 8 11105.0 9 11849.0 10 14539.0 11 15761.0 12 12583.0 13 11373.0 14 11184.0 15 10989.0 16 11470.0 17 12335.0 18 12901.0 19 14074.0 20 15150.0 21 16461.0 22 18274.0 23 20873.0 24 24647.0 25 29196.0 26 34584.0 27 41206.0 28 49079.0 29 59431.0 30 72391.0 31 88845.0 32 111208.0 33 143616.0 34 194975.0 35 264835.0 36 374118.0 37 476645.0 38 394500.0 39 99101.0 40 613.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.870086806686793 26.172976260289744 28.42832113406167 22.528615798961795 2 22.30479368617912 26.370070676072764 28.553488171722258 22.77164746602585 3 22.460330964474355 26.385167057051728 27.906256901126987 23.248245077346926 4 22.549547419653003 26.67785641901616 28.203570462674293 22.569025698656542 5 22.67930014799883 26.810198017221154 27.854299351527633 22.656202483252383 6 22.77823534629288 26.476068764402093 27.729750179658495 23.01594570964653 7 22.510645877668527 26.355507508412074 27.99726440225596 23.136582211663438 8 22.556288623812886 26.411890549767403 27.525733519812572 23.50608730660714 9 22.216099637701493 26.468355269041126 28.21334013012624 23.10220496313114 10-11 23.166881066541926 26.478915615757288 27.687164211960074 22.667039105740717 12-13 23.206461409457933 26.002480812802908 27.785610271984268 23.00544750575489 14-15 22.73065672145907 26.38843109390586 27.75890184229975 23.122010342335315 16-17 22.992845862501188 26.292220045160207 27.969826825526834 22.74510726681177 18-19 22.947991715398373 26.332698943210076 27.545666857212986 23.17364248417856 20-21 22.738724166505868 26.451376537375054 27.83010415033793 22.97979514578115 22-23 23.166503177494572 26.647238638015423 27.205207662624964 22.98105052186504 24-25 22.845202054991052 26.75909142009194 27.149484686319507 23.246221838597503 26-27 23.031509033405353 26.846468312123122 27.217224581057366 22.90479807341416 28-29 23.42410439867156 26.541413891296273 27.164401553179303 22.870080156852865 30-31 22.850643187039385 26.715973932226976 27.30406271788608 23.12932016284756 32-33 22.789787410583912 26.967923247920705 27.10033119181811 23.14195814967727 34-35 22.73505786574452 26.473777231364508 27.35135700673652 23.439807896154445 36-37 22.44189104726351 26.737096166060798 27.61864750237077 23.20236528430492 38-39 22.624484927563568 26.637338861953978 27.792234310598975 22.945941899883476 40-41 22.4184410989488 26.790483674100717 27.401495499184264 23.38957972776622 42-43 22.297858213199724 26.90120280232704 27.401468357968277 23.39947062650495 44-45 22.882784021369428 26.36858537408359 27.63055259331159 23.118078011235387 46-47 22.70373667638509 26.507209894475487 27.277636239291237 23.511417189848185 48-49 22.688665378800604 26.54149313299003 27.278914717007364 23.490926771202002 50-51 22.610312720697404 26.967079754576794 27.175545736422603 23.247061788303203 52-53 22.728392748353155 26.608731704093557 27.344759194233298 23.31811635331999 54-55 22.764972970781 26.81637781853447 27.220607704390986 23.198041506293542 56-57 23.063508174185927 26.68497493157933 27.055991539894514 23.19552535434023 58-59 22.912046874157124 26.709023036299307 27.21875849664696 23.160171592896614 60-61 22.734501421143936 26.848351514670977 27.225356082497854 23.191790981687237 62-63 22.913756893164795 26.757509229658226 27.0472932521255 23.281440625051484 64-65 22.738199393665877 26.820337446463327 26.87212946665443 23.569333693216368 66-67 22.557024838869214 26.85766545186496 27.061246715292526 23.5240629939733 68-69 22.858745346515438 26.487300103610924 27.3162779944561 23.33767655541754 70-71 22.580781854043963 26.306156508436196 27.49321098498485 23.61985065253499 72-73 22.815837558156403 25.924974096867665 27.456650173058822 23.802538171917114 74-75 22.621421493147963 26.232418442384436 27.247721527218417 23.898438537249184 76-77 22.670001054146546 25.886633240681945 27.58850031171584 23.854865393455672 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5290.0 1 3226.0 2 1084.5 3 1052.5 4 1098.0 5 1169.5 6 1260.0 7 1352.0 8 1499.0 9 1573.0 10 1673.5 11 1901.5 12 2199.5 13 2370.0 14 2542.5 15 2956.5 16 3548.5 17 3899.0 18 4321.0 19 5334.5 20 6768.0 21 8813.5 22 10017.0 23 12149.0 24 16129.5 25 20735.0 26 23492.0 27 24936.5 28 32089.5 29 38939.5 30 40081.0 31 45169.5 32 54293.5 33 62648.0 34 74681.5 35 82396.0 36 86359.5 37 93464.5 38 95454.5 39 94303.0 40 98248.0 41 105397.5 42 112932.5 43 117263.0 44 116442.5 45 118294.0 46 121558.5 47 124754.0 48 127357.0 49 128896.5 50 130060.5 51 126725.0 52 123765.0 53 124119.5 54 121156.5 55 110085.0 56 98495.5 57 94660.0 58 89514.5 59 76273.0 60 62870.5 61 57564.0 62 50416.5 63 37705.0 64 27875.5 65 23610.0 66 19746.0 67 13737.5 68 10051.5 69 7222.5 70 5935.0 71 5035.5 72 3624.0 73 2953.0 74 2794.0 75 2392.0 76 1671.0 77 1143.5 78 935.0 79 769.0 80 510.5 81 376.5 82 262.5 83 190.0 84 160.5 85 114.0 86 89.0 87 81.0 88 79.5 89 64.5 90 46.5 91 42.0 92 38.0 93 32.5 94 30.5 95 29.0 96 28.0 97 28.0 98 24.5 99 47.0 100 73.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.002642502370107434 2 0.007674116472092822 3 0.010968194769076062 4 0.017447755375229905 5 0.012633333248869788 6 0.012597134586265577 7 0.015601623582415123 8 0.017158166074396214 9 0.016144603521478295 10-11 0.0202712510583584 12-13 0.019366284493253113 14-15 0.007149235864331756 16-17 0.005266905408912762 18-19 0.010859598781263427 20-21 0.009990830878762354 22-23 0.013484001820068757 24-25 0.020796131666119464 26-27 0.012343743948036097 28-29 0.007203533858238073 30-31 0.007058739207821229 32-33 0.008560983705896 34-35 0.010027029541366564 36-37 0.008814374344125481 38-39 0.015257736287675116 40-41 0.011275883401211858 42-43 0.01447946504168457 44-45 0.016524689478822515 46-47 0.015420630269394067 48-49 0.012343743948036097 50-51 0.025031375190812204 52-53 0.029520009353734417 54-55 0.02224407817028792 56-57 0.014207975072152984 58-59 0.01498624631814353 60-61 0.018008834645595183 62-63 0.02347483269883111 64-65 0.01898619853590889 66-67 0.01777354333866781 68-69 0.027456685585294367 70-71 0.05266905408912762 72-73 0.06394493749033948 74-75 0.07516652289764503 76-77 0.07261451718404811 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 2762533.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.79270058745566 #Duplication Level Percentage of deduplicated Percentage of total 1 72.24728654505161 35.25140220648817 2 13.695024041879453 13.36434415226862 3 5.526616516298135 8.089756348242663 4 2.6958911674499038 5.261592421989978 5 1.573119810744255 3.837838195691967 6 0.9594034432020088 2.808713096803779 7 0.6570621608180249 2.2441886096098376 8 0.4635686970603286 1.809501490990524 9 0.3454467878709981 1.5169753520539127 >10 1.6853287124439829 14.438486934107079 >50 0.09795837478742374 3.2565768686229832 >100 0.0471056722648816 4.265890157205214 >500 0.0033549777808265847 1.1908514854579602 >1k 0.0027585372864201542 2.4587867409831823 >5k 7.45550617951393E-5 0.20509593948400207 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5638 0.20408805976254402 No Hit AACAAAAATGAAGTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTAC 3511 0.12709350440338632 No Hit TCAACACCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGT 3049 0.11036972228024064 No Hit ATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTAAAT 3029 0.10964574902815642 No Hit TACATCAAGTTGGGGGGACAAAAAAAAGGGAGGCCTCAGACCTGGGCCAT 2888 0.10454173760096258 No Hit TTTTTTAAATCTTCCGCCTTAATACTTCATTTTTGTTTTTAATTTCTGAA 2871 0.10392636033669099 No Hit CCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTAA 2846 0.10302139377158571 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 3.619866260421142E-5 0.0 7.239732520842285E-5 0.0 2 0.0 3.619866260421142E-5 0.0 2.5339063822948E-4 0.0 3 0.0 3.619866260421142E-5 0.0 5.429799390631714E-4 0.0 4 7.239732520842285E-5 3.619866260421142E-5 0.0 0.001447946504168457 0.0 5 2.1719197562526855E-4 3.619866260421142E-5 0.0 0.0034388729474000854 0.0 6 2.1719197562526855E-4 3.619866260421142E-5 0.0 0.006515759268758056 0.0 7 2.1719197562526855E-4 3.619866260421142E-5 0.0 0.01476905434251826 3.619866260421142E-5 8 2.1719197562526855E-4 3.619866260421142E-5 0.0 0.022732760115444776 7.239732520842285E-5 9 2.1719197562526855E-4 3.619866260421142E-5 0.0 0.03210821372993553 1.0859598781263427E-4 10 2.1719197562526855E-4 3.619866260421142E-5 0.0 0.04289541518599054 1.8099331302105713E-4 11 2.1719197562526855E-4 3.619866260421142E-5 0.0 0.05408080193069187 1.8099331302105713E-4 12 2.1719197562526855E-4 3.619866260421142E-5 0.0 0.06928424022446067 1.8099331302105713E-4 13 2.1719197562526855E-4 3.619866260421142E-5 0.0 0.09176360970167596 1.8099331302105713E-4 14 2.5339063822948E-4 3.619866260421142E-5 0.0 0.11873161334181348 1.8099331302105713E-4 15 2.5339063822948E-4 3.619866260421142E-5 0.0 0.14834211935205843 1.8099331302105713E-4 16 2.5339063822948E-4 3.619866260421142E-5 0.0 0.17845940663876234 1.8099331302105713E-4 17 2.5339063822948E-4 3.619866260421142E-5 0.0 0.20864909125067466 1.8099331302105713E-4 18 2.5339063822948E-4 3.619866260421142E-5 0.0 0.2385853852243575 1.8099331302105713E-4 19 3.9818528864632566E-4 3.619866260421142E-5 0.0 0.2737342866130468 1.8099331302105713E-4 20 3.9818528864632566E-4 3.619866260421142E-5 0.0 0.315036960644452 1.8099331302105713E-4 21 3.9818528864632566E-4 3.619866260421142E-5 0.0 0.3601766929119037 2.5339063822948E-4 22 4.343839512505371E-4 3.619866260421142E-5 0.0 0.40716255697217013 2.5339063822948E-4 23 4.7058261385474854E-4 3.619866260421142E-5 0.0 0.45581355951223024 3.6198662604211427E-4 24 5.0678127645896E-4 3.619866260421142E-5 0.0 0.5052971312921872 3.9818528864632566E-4 25 5.0678127645896E-4 3.619866260421142E-5 0.0 0.557459404104856 3.9818528864632566E-4 26 5.0678127645896E-4 3.619866260421142E-5 0.0 0.6138569204422173 4.343839512505371E-4 27 5.0678127645896E-4 3.619866260421142E-5 0.0 0.6687702916128061 4.343839512505371E-4 28 5.0678127645896E-4 3.619866260421142E-5 0.0 0.7242628413850621 5.0678127645896E-4 29 5.0678127645896E-4 3.619866260421142E-5 0.0 0.7812395363240909 5.429799390631714E-4 30 5.0678127645896E-4 3.619866260421142E-5 0.0 0.8452025731457325 5.791786016673828E-4 31 5.0678127645896E-4 3.619866260421142E-5 0.0 0.9148849986588395 6.153772642715943E-4 32 5.429799390631714E-4 3.619866260421142E-5 0.0 0.9915537660545594 6.153772642715943E-4 33 5.429799390631714E-4 3.619866260421142E-5 0.0 1.0674623615355907 6.87774589480017E-4 34 5.429799390631714E-4 3.619866260421142E-5 0.0 1.1482939751307948 7.239732520842285E-4 35 5.429799390631714E-4 3.619866260421142E-5 0.0 1.232890249636837 7.239732520842285E-4 36 5.429799390631714E-4 3.619866260421142E-5 0.0 1.322264747606635 7.601719146884399E-4 37 5.429799390631714E-4 3.619866260421142E-5 0.0 1.4092501338445549 7.601719146884399E-4 38 5.429799390631714E-4 3.619866260421142E-5 0.0 1.4981540492004983 8.687679025010742E-4 39 5.429799390631714E-4 3.619866260421142E-5 0.0 1.592451565284469 8.687679025010742E-4 40 5.429799390631714E-4 3.619866260421142E-5 0.0 1.6938802179014694 8.687679025010742E-4 41 5.429799390631714E-4 3.619866260421142E-5 0.0 1.804756721458169 8.687679025010742E-4 42 5.429799390631714E-4 3.619866260421142E-5 0.0 1.9265652211213404 8.687679025010742E-4 43 5.429799390631714E-4 3.619866260421142E-5 0.0 2.0558306452809796 9.049665651052856E-4 44 5.791786016673828E-4 3.619866260421142E-5 0.0 2.1886435383758314 9.049665651052856E-4 45 5.791786016673828E-4 3.619866260421142E-5 0.0 2.3197188956656807 9.049665651052856E-4 46 6.153772642715943E-4 3.619866260421142E-5 0.0 2.464947930033777 9.049665651052856E-4 47 6.515759268758056E-4 3.619866260421142E-5 0.0 2.6097425804506225 9.049665651052856E-4 48 6.515759268758056E-4 3.619866260421142E-5 0.0 2.7665189881894623 9.411652277094971E-4 49 6.515759268758056E-4 3.619866260421142E-5 0.0 2.9204719002451736 9.773638903137084E-4 50 6.515759268758056E-4 3.619866260421142E-5 0.0 3.082822902025062 9.773638903137084E-4 51 6.515759268758056E-4 3.619866260421142E-5 0.0 3.2565402838626722 0.00101356255291792 52 6.515759268758056E-4 3.619866260421142E-5 0.0 3.4368458222942495 0.0010497612155221314 53 6.515759268758056E-4 3.619866260421142E-5 0.0 3.6387619623005407 0.0010497612155221314 54 6.515759268758056E-4 3.619866260421142E-5 0.0 3.8461802990226723 0.0010497612155221314 55 6.515759268758056E-4 3.619866260421142E-5 0.0 4.057906276594705 0.0010497612155221314 56 6.515759268758056E-4 3.619866260421142E-5 0.0 4.285921652338633 0.0010497612155221314 57 6.515759268758056E-4 3.619866260421142E-5 0.0 4.507421268813802 0.0010859598781263427 58 6.515759268758056E-4 3.619866260421142E-5 0.0 4.752558611969522 0.0011583572033347655 59 6.515759268758056E-4 3.619866260421142E-5 0.0 4.980537789050846 0.0011583572033347655 60 6.515759268758056E-4 3.619866260421142E-5 0.0 5.2296207864304245 0.001194555865938977 61 6.87774589480017E-4 3.619866260421142E-5 0.0 5.490432150493768 0.0012307545285431885 62 6.87774589480017E-4 3.619866260421142E-5 0.0 5.753849818264614 0.0012669531911474 63 6.87774589480017E-4 3.619866260421142E-5 0.0 6.026389549011722 0.0014117478415642455 64 6.87774589480017E-4 3.619866260421142E-5 0.0 6.308232336048112 0.0014117478415642455 65 6.87774589480017E-4 3.619866260421142E-5 0.0 6.6072332891588985 0.0014117478415642455 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCGCG 95 2.5318403E-4 22.416222 5 ACGCGTG 90 0.0048624715 19.722614 19 CGCGGAA 185 1.9571962E-7 19.191307 59 AATTCGC 190 2.5958434E-7 18.68289 55 CCGCGAC 95 0.006655209 18.679846 34 CATAACG 100 0.008940795 17.747461 26 GACGTAA 305 5.7661964E-10 16.291277 31 AGTGTAC 1380 0.0 15.690069 11 CAATTCG 215 1.7296712E-5 14.860223 54 ATACGTA 215 1.7338203E-5 14.856456 1 ACGACCG 170 7.343858E-4 14.618467 22 TCGCGGA 245 3.6875936E-6 14.490871 58 ATTCGCG 225 2.6580383E-5 14.198997 56 ACGAGAC 375 6.9849193E-10 14.196941 27 CAGTGTA 1680 0.0 14.155969 10 GCCGTAT 265 8.262548E-6 13.403774 68 CCCGATA 700 0.0 13.184783 38 ACGTAAT 405 2.2118911E-9 13.144839 32 GTGTACA 1715 0.0 13.040132 12 CGTAGTA 305 2.5075715E-6 12.802375 38 >>END_MODULE