FastQCFastQC Report
Thu 26 May 2016
SRR936160_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936160_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2762533
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63370.2293909249228878No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44140.15978089673498924No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA37430.13549159412756334No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG33190.12014336118337772No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32310.11695787887420711No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA30490.11036972228024064No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA28560.10338338039762782No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC28120.10179063924304252No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA12150.041.393353100-101
GGTATCA58150.040.8340261
CCGTACA8100.038.94404100-101
CCTGTCG9050.035.8382696-97
GTATCAA119850.032.225951
CGTACAT18450.032.097916100-101
TTATACG7050.030.38872196-97
ACGTATC4700.029.759125100-101
TACGCAG7100.029.339983100-101
TAAATCG2750.029.2146196-97
TCGAGTA4500.029.097809100-101
GCGTACA5750.028.982798100-101
AGGTATC17850.028.175306100-101
AGTATCA26750.026.699774100-101
CCGTATC7150.026.221325100-101
CGAGTAC12300.026.129961100-101
CCCGTAC3450.025.87585898-99
ATACACG8700.025.3093896-97
TATACGT5450.024.56886196-97
TATCAAC157250.024.176822
Produced by FastQC (version 0.11.5)