FastQCFastQC Report
Thu 26 May 2016
SRR936159_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936159_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3152193
Sequences flagged as poor quality0
Sequence length77
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88240.27993209806633035No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAC3800.024.282594
TTACGCG1200.001220174517.74975856
TCGCGCT4000.017.74834837
GCGGTTA5450.016.9307123
CGCGGAA3451.0913936E-1116.46432759
CTCGCGC4350.016.31902736
AGTGTAC13700.015.80359511
CGCTCCG4550.015.60343925
GTAACGT2754.6520654E-815.4867652
AATTCGC4600.015.43383655
CTACGGT1400.003422197415.21118135
TAAGGCG15200.015.18047317
CGCGCTT4800.014.78864938
TCGCGGA4351.8189894E-1214.68968858
ATTCGCG4401.8189894E-1214.52253156
GGGCGGT6950.014.2987871
GTTACGC1500.005407459614.19913155
GACGCAT2809.560699E-713.948894548
GTACATA17900.013.88491647
GACCGTA1550.006689515413.75047769
Produced by FastQC (version 0.11.5)