##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936159_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3152193 Sequences flagged as poor quality 0 Sequence length 77 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.693244036770594 40.0 36.0 41.0 30.0 41.0 2 36.54572070936012 39.0 36.0 41.0 30.0 41.0 3 36.454873162905955 39.0 36.0 41.0 30.0 41.0 4 36.33983610775102 39.0 36.0 41.0 30.0 41.0 5 36.257237421693404 39.0 36.0 41.0 29.0 41.0 6 36.157062400684225 39.0 35.0 41.0 29.0 41.0 7 35.9746478086843 39.0 35.0 40.0 29.0 41.0 8 35.79945993154607 39.0 35.0 40.0 28.0 41.0 9 35.69244046922254 38.0 35.0 40.0 27.0 41.0 10-11 35.899559449564165 39.0 35.0 40.0 28.5 41.0 12-13 36.0783887598253 39.0 35.0 41.0 29.0 41.0 14-15 36.013430173850395 39.0 35.0 41.0 28.5 41.0 16-17 35.83042551645791 39.0 35.0 41.0 28.0 41.0 18-19 35.65622758504952 39.0 35.0 41.0 27.0 41.0 20-21 35.431343512278595 39.0 35.0 41.0 26.5 41.0 22-23 35.14822553695158 39.0 35.0 41.0 25.5 41.0 24-25 34.890099210295816 39.0 34.0 41.0 24.5 41.0 26-27 33.916406133761484 38.0 33.0 39.5 23.0 40.5 28-29 33.92341553959418 38.0 33.0 39.5 23.0 40.5 30-31 34.26485561004672 38.0 34.0 40.0 23.0 41.0 32-33 34.069113629780915 38.0 33.0 40.0 22.5 41.0 34-35 33.764740927982515 37.5 33.0 40.0 21.5 41.0 36-37 33.447050830961174 37.0 33.0 40.0 20.5 41.0 38-39 33.140159406483036 36.5 33.0 40.0 20.0 41.0 40-41 32.737643443786595 36.0 32.0 39.0 18.0 41.0 42-43 32.361596831158494 35.5 32.0 39.0 17.0 41.0 44-45 31.976161040900735 35.0 31.5 38.5 13.5 40.0 46-47 31.574042420625894 35.0 31.0 37.5 9.5 40.0 48-49 31.116581694077745 35.0 31.0 37.0 7.0 39.5 50-51 30.695977213324184 35.0 30.5 36.5 4.0 39.0 52-53 30.265127484262543 34.5 30.0 36.0 2.0 39.0 54-55 29.826631015296336 34.0 30.0 35.5 2.0 37.5 56-57 29.40232879141601 34.0 29.0 35.0 2.0 37.0 58-59 29.000961076939134 34.0 29.0 35.0 2.0 36.5 60-61 28.67175439448029 34.0 29.0 35.0 2.0 36.0 62-63 28.352689540266095 34.0 29.0 35.0 2.0 36.0 64-65 27.994243848647592 34.0 27.5 35.0 2.0 35.5 66-67 27.626564109494566 34.0 27.0 35.0 2.0 35.0 68-69 27.276468794899298 33.0 26.5 35.0 2.0 35.0 70-71 26.85809767993267 33.0 25.5 35.0 2.0 35.0 72-73 26.3781216759253 33.0 25.0 35.0 2.0 35.0 74-75 25.797872465296383 33.0 23.5 35.0 2.0 35.0 76-77 24.08887495150202 31.0 10.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.8414017420431747 1101 2 0.6848280333621233 1101 3 0.9480800271992891 1101 4 0.9610869162174183 1101 5 0.9059799842351666 1101 6 0.8344080643269223 1101 7 0.8467289479258397 1101 8 1.0050346761072433 1101 9 0.9977451398908457 1101 10-11 0.9032556161336913 1101 12-13 0.93983082239901 1101 14-15 1.0598551450263756 1101 16-17 1.1167410222132688 1101 18-19 1.073678233335265 1101 20-21 0.8574433942249513 1101 22-23 0.8572937065208492 1101 24-25 0.8366021879344459 1101 26-27 0.6566809810366507 1101 28-29 0.7644009631178932 1101 30-31 0.8442450801684487 1101 32-33 0.8145398856268145 1101 34-35 0.7986466177769884 1101 36-37 0.8235468336210516 1101 38-39 0.7915809758959327 1101 40-41 0.7541359028355785 1101 42-43 0.7998011856925444 1101 44-45 0.6879951711207539 1101 46-47 0.662290006295887 1101 48-49 0.8412229516248289 1101 50-51 0.6870839243239786 1101 52-53 0.7938163381618892 1101 54-55 0.6513037980571177 1101 56-57 0.6936073919634715 1101 58-59 0.7400401646765467 1101 60-61 0.6693167749706888 1101 62-63 0.534347246043712 1101 64-65 0.5178747601774738 1101 66-67 0.5481597871055222 1101 68-69 0.5576521701645767 1101 70-71 0.7270086189040086 1101 72-73 0.8073514073029209 1101 74-75 0.8602074023049759 1101 76-77 1.0203045706600022 1102 1 0.9382306932353401 1102 2 0.95650508059434 1102 3 0.9344632428845898 1102 4 0.9288291855175217 1102 5 1.0620298396815429 1102 6 0.8635162585151406 1102 7 0.8474362848849779 1102 8 0.8046830042414683 1102 9 0.9806593608577998 1102 10-11 0.8826879983118658 1102 12-13 1.0137281772144462 1102 14-15 0.9805097738163013 1102 16-17 0.9773678225897484 1102 18-19 1.011958944329436 1102 20-21 1.032896135533079 1102 22-23 0.993165953430875 1102 24-25 0.8784647266751406 1102 26-27 0.8143385366919631 1102 28-29 0.8689437221240581 1102 30-31 0.9006447934839272 1102 32-33 0.937981689874178 1102 34-35 0.9948279234577129 1102 36-37 1.1547601039928779 1102 38-39 1.055875543633313 1102 40-41 1.129564307369499 1102 42-43 1.1498445329084248 1102 44-45 1.201047120698469 1102 46-47 1.0771990136659504 1102 48-49 1.093018777898493 1102 50-51 1.2170378792445788 1102 52-53 1.1307665252044252 1102 54-55 1.0760247621488688 1102 56-57 1.3338949554822683 1102 58-59 1.2482092978308401 1102 60-61 1.229287931206084 1102 62-63 1.2487449741894032 1102 64-65 1.294392987130859 1102 66-67 1.228139424079739 1102 68-69 1.1153855971439661 1102 70-71 1.1688843304259429 1102 72-73 1.3971438775609855 1102 74-75 1.484566005342483 1102 76-77 1.4500954119733969 1103 1 0.7134080952670274 1103 2 0.6418443683563098 1103 3 0.7882039500775946 1103 4 0.7604102254527731 1103 5 0.6838146385387986 1103 6 0.8383361418615749 1103 7 0.7392277575357298 1103 8 0.8613596555459608 1103 9 0.6580842430939171 1103 10-11 0.8406848627147738 1103 12-13 0.889480947829135 1103 14-15 0.9515090804243087 1103 16-17 0.9128790861227358 1103 18-19 0.9707218690618618 1103 20-21 0.9768026208065308 1103 22-23 0.89649582199187 1103 24-25 0.8210216570690179 1103 26-27 0.7067595629717687 1103 28-29 0.7409027042470484 1103 30-31 1.0334343114875963 1103 32-33 1.0098629085210433 1103 34-35 1.0526129160972104 1103 36-37 1.1050589570144211 1103 38-39 0.9445870241928631 1103 40-41 1.0204598354744263 1103 42-43 1.0076498344993396 1103 44-45 0.9746129919232445 1103 46-47 0.7512744701311114 1103 48-49 0.831838204949797 1103 50-51 0.8327410898129628 1103 52-53 0.8539896440937689 1103 54-55 0.9289362269337822 1103 56-57 0.9452034020372118 1103 58-59 0.8822424109675708 1103 60-61 0.8713891988927394 1103 62-63 0.7364372365385776 1103 64-65 0.6893409982437468 1103 66-67 0.6317761940686921 1103 68-69 0.6285261569240568 1103 70-71 0.7588298959926512 1103 72-73 0.7991110059361084 1103 74-75 0.7836701443234837 1103 76-77 0.8455841399684054 1104 1 0.6343618264628788 1104 2 0.550737531171599 1104 3 0.5118811666281502 1104 4 0.5240031901309052 1104 5 0.6153538911956389 1104 6 0.4559609363160604 1104 7 0.7356134103134266 1104 8 0.7065711858504855 1104 9 0.6870439939407973 1104 10-11 0.6055132503663572 1104 12-13 0.6989820605348953 1104 14-15 0.6339279807340077 1104 16-17 0.6987834573422944 1104 18-19 0.8154318392541313 1104 20-21 0.8105763493295584 1104 22-23 0.8370721047992546 1104 24-25 0.8553350624134879 1104 26-27 0.6854694634383449 1104 28-29 0.7083107099180666 1104 30-31 0.7044947229853662 1104 32-33 0.7574090291580973 1104 34-35 0.8903712933480392 1104 36-37 0.702611992115493 1104 38-39 0.8002484013914 1104 40-41 0.7958097131639548 1104 42-43 0.8106942323308743 1104 44-45 0.672906571930568 1104 46-47 0.6104995853237547 1104 48-49 0.8293284288273597 1104 50-51 0.6539907484476188 1104 52-53 0.49775744593336313 1104 54-55 0.6032365587118456 1104 56-57 0.7321836987200108 1104 58-59 0.6453976145375293 1104 60-61 0.5044629977225092 1104 62-63 0.4076592373171799 1104 64-65 0.4932441260218816 1104 66-67 0.4573224076733382 1104 68-69 0.31380229969902373 1104 70-71 0.30016386460447464 1104 72-73 0.19627665673824524 1104 74-75 0.1201513793978286 1104 76-77 0.022721203238656784 1105 1 0.28217163637520315 1105 2 0.5529855839250573 1105 3 0.5649091990130373 1105 4 0.6089271493710058 1105 5 0.5001810143913445 1105 6 0.33451215074929763 1105 7 0.43834313988938334 1105 8 0.7373215798514323 1105 9 0.48265155193147535 1105 10-11 0.4501339568939642 1105 12-13 0.5870482982099219 1105 14-15 0.3330985589623978 1105 16-17 0.48840686640345865 1105 18-19 0.6795061219448257 1105 20-21 0.611413736463227 1105 22-23 0.6845923169054657 1105 24-25 0.5940838178256698 1105 26-27 0.4714562864536873 1105 28-29 0.6187183657689133 1105 30-31 0.5730698335047606 1105 32-33 0.7520499904635045 1105 34-35 0.7639865436026483 1105 36-37 0.6302194249362003 1105 38-39 0.6798751103882736 1105 40-41 0.7313501345316027 1105 42-43 0.5793519550338573 1105 44-45 0.4594858471964116 1105 46-47 0.4482927248712123 1105 48-49 0.5244131176946283 1105 50-51 0.6359144402791408 1105 52-53 0.4776495152775304 1105 54-55 0.5919310358711662 1105 56-57 0.7413592837474035 1105 58-59 0.6399919784749173 1105 60-61 0.3049005236810558 1105 62-63 0.23332966477264705 1105 64-65 0.46875530313170444 1105 66-67 0.5797087163538741 1105 68-69 0.3659813382798198 1105 70-71 0.08659885302624915 1105 72-73 0.03848966189866232 1105 74-75 0.10213594935362025 1105 76-77 0.47685016245031164 1106 1 0.16873235248698393 1106 2 0.2055721961141046 1106 3 -0.015702540017578315 1106 4 -0.022128523719374016 1106 5 -0.10991858836839441 1106 6 0.01559101067065427 1106 7 0.15298813898843378 1106 8 0.2656419843541542 1106 9 0.12224073342262187 1106 10-11 0.00466937191994532 1106 12-13 0.1357399000952384 1106 14-15 0.1485724872689005 1106 16-17 0.2836158131359454 1106 18-19 0.15490870158267 1106 20-21 0.08757503311471737 1106 22-23 0.10388270841076519 1106 24-25 0.23782524853088916 1106 26-27 0.09281036308273372 1106 28-29 0.17284183724453328 1106 30-31 0.05463338737366996 1106 32-33 -0.030379845362752178 1106 34-35 0.079286570676814 1106 36-37 0.10408719185686977 1106 38-39 0.035194944595581035 1106 40-41 -0.07238591792643234 1106 42-43 -0.08849583371078751 1106 44-45 -0.09044815951542162 1106 46-47 0.04024800355679403 1106 48-49 -0.14787727015199437 1106 50-51 -0.2641022409764773 1106 52-53 -0.09563584829838234 1106 54-55 0.02118742002436491 1106 56-57 -0.043874237824578444 1106 58-59 -0.10010474079430054 1106 60-61 -0.2031681619320409 1106 62-63 -0.14163855051821983 1106 64-65 -0.034099775516015285 1106 66-67 -0.019466836311330837 1106 68-69 -0.12106896525271438 1106 70-71 -0.2739113212820108 1106 72-73 -0.13121953316615276 1106 74-75 -0.28231559977364995 1106 76-77 -0.2847567443898491 1107 1 0.0422705063153046 1107 2 0.0937950362341553 1107 3 0.07583966755743887 1107 4 0.06338791647559816 1107 5 0.15005110887157969 1107 6 -0.08939233284535675 1107 7 -0.015644044817086922 1107 8 0.12171390825609762 1107 9 -0.028514828772735257 1107 10-11 0.04555652479837846 1107 12-13 0.28444601237822553 1107 14-15 0.3112693771666457 1107 16-17 0.29763502049245005 1107 18-19 0.3628850279694831 1107 20-21 0.2764483568748446 1107 22-23 0.36565819595296034 1107 24-25 0.2825252757342511 1107 26-27 0.13403031117337605 1107 28-29 0.3957982962588886 1107 30-31 0.572562353155412 1107 32-33 0.5256941410890832 1107 34-35 0.5095482469389836 1107 36-37 0.6235665262218504 1107 38-39 0.5552327282798046 1107 40-41 0.5916093192556602 1107 42-43 0.6102573270075595 1107 44-45 0.6509986133186842 1107 46-47 0.6721001845081993 1107 48-49 0.8224771985574506 1107 50-51 0.689312393985638 1107 52-53 0.7187952001013009 1107 54-55 0.7328509724265082 1107 56-57 0.7823623444522063 1107 58-59 0.7387566536232129 1107 60-61 0.6161776083913217 1107 62-63 0.7207647326152475 1107 64-65 0.6419716912802791 1107 66-67 0.6901044543320509 1107 68-69 0.7032017282545979 1107 70-71 0.7118285432363329 1107 72-73 0.5656599960819193 1107 74-75 0.346934646617445 1107 76-77 0.44306961789648014 1108 1 0.3024428256852971 1108 2 0.31717288024148615 1108 3 0.2894975187268116 1108 4 0.3248403706010379 1108 5 0.2681289954625612 1108 6 0.24029245891221507 1108 7 0.10898437217919366 1108 8 0.2078058584768172 1108 9 0.13476882714090976 1108 10-11 -0.010957171540852073 1108 12-13 0.029734848689521698 1108 14-15 0.2097133675955618 1108 16-17 0.12074775373767466 1108 18-19 0.11943112148443902 1108 20-21 0.0967654816438781 1108 22-23 0.15717291881534123 1108 24-25 0.14869112512045746 1108 26-27 -0.01176989644159221 1108 28-29 0.13494148810004702 1108 30-31 0.2679900982647254 1108 32-33 0.2626186777228341 1108 34-35 0.2863695641755939 1108 36-37 0.2661092161955736 1108 38-39 0.2761384629999668 1108 40-41 0.33778542862264516 1108 42-43 0.19663491845344083 1108 44-45 0.1641523917377512 1108 46-47 0.15760746284614768 1108 48-49 0.20080926383277387 1108 50-51 0.179381688236532 1108 52-53 0.2570578923643616 1108 54-55 0.41354356838542117 1108 56-57 0.2994764019385521 1108 58-59 0.17145863583679954 1108 60-61 0.11978792445018627 1108 62-63 0.13693869442228745 1108 64-65 0.11351872730625345 1108 66-67 0.09587109910305713 1108 68-69 0.15820625178245606 1108 70-71 0.042062039185637445 1108 72-73 0.053495407927730554 1108 74-75 0.024440977718683143 1108 76-77 -0.008005973588257831 1109 1 0.07505812498128961 1109 2 0.20183430966600469 1109 3 0.11460069731828071 1109 4 0.2498006780342834 1109 5 0.10145680827201176 1109 6 0.22539335358408863 1109 7 0.13577184683604315 1109 8 0.11902100780383051 1109 9 0.1821626824508158 1109 10-11 0.1582673904371319 1109 12-13 0.164182633839566 1109 14-15 0.283548166391391 1109 16-17 0.41307274287802187 1109 18-19 0.33330394564880805 1109 20-21 0.2847866388150848 1109 22-23 0.2941131416363021 1109 24-25 0.18808053625090793 1109 26-27 0.12595171710344033 1109 28-29 0.29957581332956806 1109 30-31 0.4706201389367948 1109 32-33 0.5522482316424231 1109 34-35 0.6317165038652917 1109 36-37 0.5385006976802131 1109 38-39 0.45885897086292715 1109 40-41 0.6074934623098258 1109 42-43 0.5838886281666333 1109 44-45 0.4463803426864743 1109 46-47 0.24410034972997963 1109 48-49 0.3383575329277093 1109 50-51 0.4163549080174178 1109 52-53 0.48136876536739237 1109 54-55 0.5222332199898894 1109 56-57 0.48046567351016023 1109 58-59 0.5686964495957731 1109 60-61 0.47221627672522715 1109 62-63 0.45690699715141037 1109 64-65 0.3091815513728342 1109 66-67 0.3355982716579149 1109 68-69 0.5039386817688083 1109 70-71 0.48579291648521306 1109 72-73 0.42059210763236976 1109 74-75 0.286372012810606 1109 76-77 0.30748218741166866 1110 1 -0.40955188694236 1110 2 -0.4617740250687703 1110 3 -0.5088584327555168 1110 4 -0.2912818637982184 1110 5 -0.2874196064931098 1110 6 -0.3191392177671446 1110 7 -0.5461739548637681 1110 8 -0.34168767393379795 1110 9 -0.4300284251655242 1110 10-11 -0.28459460787709645 1110 12-13 -0.26667279308605174 1110 14-15 -0.3930673967698439 1110 16-17 -0.3702458184666426 1110 18-19 -0.2838901946441723 1110 20-21 -0.44486278780448885 1110 22-23 -0.5727892203163094 1110 24-25 -0.6181934429794254 1110 26-27 -0.3826815381024886 1110 28-29 -0.3386230343038079 1110 30-31 -0.24701529242295805 1110 32-33 -0.13533365188966684 1110 34-35 -0.03426661112037266 1110 36-37 -0.07922159802318163 1110 38-39 -0.05625390985667167 1110 40-41 -0.10237210501787075 1110 42-43 -0.06288049802351736 1110 44-45 -0.14151561605094187 1110 46-47 -0.24228932068549014 1110 48-49 -0.03381486855322535 1110 50-51 -0.19946071534282694 1110 52-53 -0.10302837841178203 1110 54-55 0.05634903945144387 1110 56-57 -0.12407207940017528 1110 58-59 -0.1601493808399752 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-0.7039303388248399 2314 26-27 -0.7120822804772615 2314 28-29 -0.7345502781276565 2314 30-31 -0.8405114867361192 2314 32-33 -0.8901423986262245 2314 34-35 -0.931983568525176 2314 36-37 -1.149413867422865 2314 38-39 -1.0030299250999448 2314 40-41 -0.8784705688251471 2314 42-43 -0.7216286099602698 2314 44-45 -0.7814114651651778 2314 46-47 -0.7988835522339919 2314 48-49 -0.7439848527507635 2314 50-51 -0.6225237235905681 2314 52-53 -0.588168525154174 2314 54-55 -0.7181785803633396 2314 56-57 -0.5790454196801846 2314 58-59 -0.6036447335049431 2314 60-61 -0.505807204195051 2314 62-63 -0.4989324610755155 2314 64-65 -0.36713992831657194 2314 66-67 -0.5120233360706514 2314 68-69 -0.3897782031303514 2314 70-71 -0.3134166833865102 2314 72-73 -0.32793945060187113 2314 74-75 -0.27356469858724 2314 76-77 -0.23061005266057677 2315 1 -1.1778999898628157 2315 2 -1.0030639736980334 2315 3 -1.051114809144451 2315 4 -0.96719172740206 2315 5 -0.906587490896321 2315 6 -0.9741052834167832 2315 7 -1.0282821147274745 2315 8 -0.9745854946860177 2315 9 -0.8542734733936896 2315 10-11 -0.916724001309646 2315 12-13 -0.9309849435349022 2315 14-15 -1.232465299763298 2315 16-17 -1.2457318807520537 2315 18-19 -1.0575741173316118 2315 20-21 -1.0591408609648028 2315 22-23 -1.1230114788139787 2315 24-25 -1.1576335230910857 2315 26-27 -1.1251702200924 2315 28-29 -1.0805620050909823 2315 30-31 -1.232018053822685 2315 32-33 -1.1490151964173947 2315 34-35 -1.1421238187879865 2315 36-37 -1.3733547111626763 2315 38-39 -1.170088211786009 2315 40-41 -1.2456529544932025 2315 42-43 -1.4958600628368686 2315 44-45 -1.386996263258407 2315 46-47 -1.1882482100103253 2315 48-49 -1.0732876380030731 2315 50-51 -1.1214738151082848 2315 52-53 -1.2505737985692171 2315 54-55 -1.3101093379890223 2315 56-57 -1.3275060757318435 2315 58-59 -1.168688548591799 2315 60-61 -1.3078557867147147 2315 62-63 -1.4618187980577382 2315 64-65 -1.4348255664870422 2315 66-67 -1.422875294460919 2315 68-69 -1.496728870972884 2315 70-71 -1.5413686231701682 2315 72-73 -2.824914569261086 2315 74-75 -3.387425425010136 2315 76-77 -2.9571929344236487 2316 1 -3.1467146387978246 2316 2 -3.185450850249026 2316 3 -2.7633342558068392 2316 4 -2.61050321431987 2316 5 -3.213195727124969 2316 6 -2.7088158782783225 2316 7 -3.1403291437039087 2316 8 -3.2099251267384616 2316 9 -2.537137659571947 2316 10-11 -2.6092363938308267 2316 12-13 -2.747219259170109 2316 14-15 -2.8104839742407606 2316 16-17 -4.041151851235789 2316 18-19 -3.747999393764374 2316 20-21 -3.067889170142557 2316 22-23 -3.208046280502849 2316 24-25 -3.217230599379757 2316 26-27 -3.0750907642754584 2316 28-29 -3.2321059028390806 2316 30-31 -3.411280927163425 2316 32-33 -3.4512246880110027 2316 34-35 -3.419442395356839 2316 36-37 -3.2388849673876763 2316 38-39 -3.216523496654915 2316 40-41 -3.2721887342419222 2316 42-43 -3.520338242762932 2316 44-45 -3.2905375993723176 2316 46-47 -2.990796830771469 2316 48-49 -3.1795989873972488 2316 50-51 -3.5944644203969887 2316 52-53 -4.541405447067024 2316 54-55 -3.8098324023439183 2316 56-57 -3.6395396807598708 2316 58-59 -3.663395243529493 2316 60-61 -3.5143777319967313 2316 62-63 -3.607434565244599 2316 64-65 -3.443417796607182 2316 66-67 -3.5714497491386687 2316 68-69 -3.669978266997834 2316 70-71 -3.470031503489718 2316 72-73 -3.379495111925227 2316 74-75 -3.2974278675940205 2316 76-77 -3.4370070927892904 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 72948.0 3 6923.0 4 8986.0 5 10455.0 6 11987.0 7 13221.0 8 15282.0 9 15999.0 10 19540.0 11 21404.0 12 17329.0 13 15443.0 14 15082.0 15 15601.0 16 15905.0 17 16387.0 18 17588.0 19 18658.0 20 19969.0 21 21237.0 22 23824.0 23 26975.0 24 31394.0 25 37234.0 26 43720.0 27 51637.0 28 60467.0 29 72004.0 30 86359.0 31 104090.0 32 127295.0 33 161485.0 34 217396.0 35 294780.0 36 409634.0 37 518073.0 38 413241.0 39 102001.0 40 640.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 23.343511392255664 25.672567275763846 28.118315493175817 22.865605838804676 2 22.887591063939013 26.041597591701677 28.18274435721653 22.88806698714278 3 22.895982587617155 25.988298675668986 27.647964007640123 23.467754729073732 4 22.94474237454414 26.147348168285134 28.000194178084147 22.907715279086577 5 23.098444260987485 26.357837105869823 27.69281436023826 22.85090427290443 6 23.221403346128866 26.019986160645026 27.49767519776286 23.26093529546325 7 23.00089379738879 25.873705191544556 27.78789746935087 23.33750354171578 8 23.056208823495815 25.977321272871954 27.44425505667461 23.522214846957617 9 22.765189061312142 25.984396873282662 27.84382024040542 23.406593824999778 10-11 23.4662223617638 26.024867769105082 27.49869549952492 23.010214369606192 12-13 23.605380758993963 25.721342197723363 27.503024943748816 23.170252099533855 14-15 23.176054445596126 25.975049469894035 27.52832641214854 23.320569672361298 16-17 23.4425709668664 25.865174018311972 27.672862293132116 23.019392721689517 18-19 23.325749346402688 25.95340171243368 27.28026890498197 23.440580036181665 20-21 23.24175372605067 26.030044491436012 27.447433563506678 23.280768219006642 22-23 23.630050323759193 26.22992876234712 26.960042912987127 23.179978000906555 24-25 23.459505219715755 26.39530480217657 26.689317879190327 23.455872098917347 26-27 23.46942207766451 26.444044266391654 26.816580064257668 23.269953591686168 28-29 23.826933452160358 26.17454977052879 26.789238497608046 23.2092782797028 30-31 23.42710945511031 26.346757388549907 26.75992035290415 23.46621280343564 32-33 23.309477623731464 26.61329828108175 26.64894383200301 23.428280263183776 34-35 23.342793460931365 26.171900406907113 26.882599763629006 23.602706368532516 36-37 23.19161627250287 26.381328882994588 27.078005723677112 23.349049120825427 38-39 23.202852756191067 26.287289097929122 27.266773887216633 23.243084258663178 40-41 23.07983224404915 26.386820652885195 26.96643437382063 23.566912729245022 42-43 23.010072389848606 26.563917396567234 26.861996043386316 23.564014170197847 44-45 23.35405043143567 26.107525530702127 27.19428875024609 23.344135287616112 46-47 23.238903768535632 26.156372262265315 26.92990234646767 23.674821622731383 48-49 23.138657005436425 26.3001925317971 26.80174301963158 23.759407443134897 50-51 23.049337088559408 26.648733658490762 26.662459518630115 23.63946973431971 52-53 23.212970897203974 26.344711034942396 26.828477577834903 23.613840490018724 54-55 23.123596692943448 26.505877090809506 26.819830724933492 23.550695491313558 56-57 23.350123099212666 26.525524325857692 26.624555810000434 23.499796764929208 58-59 23.318919714727116 26.51640458693211 26.728372988235684 23.436302710105092 60-61 23.126383853409425 26.5413009443679 26.632891494469664 23.699423707753006 62-63 23.18392622832473 26.412246199638567 26.756860959368144 23.64696661266856 64-65 23.09709761930148 26.45626754806838 26.560953040988228 23.885681791641915 66-67 22.948438369692358 26.56104324050903 26.709571225205934 23.780947164592682 68-69 23.099819679943405 26.253825830471218 26.839584338704004 23.806770150881377 70-71 22.91924670314785 26.029510935386917 27.07920587012757 23.972036491337665 72-73 23.05036009142669 25.64867339374997 27.13404755210383 24.166918962719507 74-75 22.730696176607495 26.009572610927407 26.957593854325363 24.302137358139735 76-77 22.673431471265616 25.685950995811375 27.34644633421196 24.294171198711055 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 8537.0 1 5171.5 2 1696.5 3 1639.0 4 1691.0 5 1767.0 6 1894.0 7 2041.5 8 2161.5 9 2185.0 10 2297.5 11 2535.5 12 2839.0 13 3017.0 14 3173.0 15 3607.0 16 4221.5 17 4558.0 18 4956.5 19 5964.0 20 7406.5 21 9314.0 22 10388.0 23 12431.0 24 16129.0 25 20646.5 26 23509.0 27 24984.5 28 31950.0 29 39199.0 30 40958.0 31 46378.0 32 55642.0 33 63939.5 34 75712.5 35 83032.0 36 87684.5 37 95325.5 38 98888.0 39 99462.0 40 103652.5 41 111239.5 42 119357.5 43 124079.0 44 125668.5 45 130966.0 46 137207.0 47 143434.5 48 147129.0 49 148854.0 50 151627.0 51 150779.0 52 148883.0 53 149953.0 54 147119.5 55 137614.5 56 125818.0 57 119623.0 58 113668.0 59 98497.0 60 82236.5 61 75192.0 62 66124.0 63 49296.0 64 35870.0 65 30204.0 66 25425.5 67 18188.0 68 13455.5 69 9621.5 70 8061.0 71 6559.5 72 4189.0 73 2860.0 74 2400.0 75 2118.0 76 1511.0 77 939.5 78 693.0 79 581.5 80 388.0 81 271.0 82 187.5 83 139.0 84 134.0 85 105.5 86 86.5 87 91.0 88 86.0 89 76.0 90 59.5 91 48.0 92 51.0 93 54.5 94 52.5 95 53.5 96 57.0 97 46.5 98 51.0 99 114.0 100 162.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00999304293867793 2 0.013450953034918863 3 0.013101989630711063 4 0.014180603789171539 5 0.012023375472250589 6 0.009897871101166711 7 0.01497370243509836 8 0.016401279997766634 9 0.016401279997766634 10-11 0.01914540131267343 12-13 0.015275079920550551 14-15 0.008755809051032092 16-17 0.006709614544540895 18-19 0.018923333691813923 20-21 0.025379156669658235 22-23 0.02219090011303242 24-25 0.020065395741948544 26-27 0.025950187694725547 28-29 0.008644775240602335 30-31 0.009168220346914037 32-33 0.010373730288722804 34-35 0.011674412068042789 36-37 0.011833031797228152 38-39 0.013768192493289592 40-41 0.01118269090756816 42-43 0.016115764485232976 44-45 0.014228189707927148 46-47 0.02526812285922848 48-49 0.024284680538279223 50-51 0.03827494065242833 52-53 0.041019061967335124 54-55 0.029550855547233305 56-57 0.02125504371083877 58-59 0.017257826535367598 60-61 0.021159871873327553 62-63 0.026901906069837726 64-65 0.0269336300156748 66-67 0.029661889357663063 68-69 0.044619729819842885 70-71 0.05715068842548663 72-73 0.08833532718332919 74-75 0.08854153283127017 76-77 0.09364908811103889 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 3152193.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.47483725279279 #Duplication Level Percentage of deduplicated Percentage of total 1 71.32569781969114 33.14850198112024 2 13.488558810232757 12.537571509605835 3 5.616030052290499 7.83012248060983 4 2.906020172827469 5.402272583419576 5 1.736342943297398 4.03481278523909 6 1.0663969030329448 2.973637350920302 7 0.733424260760131 2.3860041209253864 8 0.5476325866364133 2.036090827060261 9 0.39509820811682317 1.6525912428989473 >10 2.0010668167275014 16.478576530406787 >50 0.12095561981991745 3.836022322873746 >100 0.0567231621837863 4.607188885681154 >500 0.004058022939250749 1.3020792640158623 >1k 0.001925841394303355 1.4924652826271236 >5k 6.878004979654838E-5 0.2820628325958822 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8824 0.27993209806633035 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.1723945837072795E-5 0.0 2 0.0 0.0 0.0 1.2689578334829118E-4 6.344789167414559E-5 3 0.0 0.0 0.0 3.1723945837072796E-4 6.344789167414559E-5 4 0.0 0.0 0.0 0.0011737859959716935 6.344789167414559E-5 5 0.0 0.0 0.0 0.0025061917211287505 6.344789167414559E-5 6 0.0 0.0 0.0 0.005012383442257501 6.344789167414559E-5 7 0.0 0.0 0.0 0.009390287967773548 6.344789167414559E-5 8 0.0 0.0 0.0 0.01475163481423885 6.344789167414559E-5 9 0.0 0.0 0.0 0.0212233197650017 6.344789167414559E-5 10 0.0 0.0 0.0 0.027885348390786987 1.5861972918536398E-4 11 0.0 0.0 0.0 0.03578461090421811 1.5861972918536398E-4 12 0.0 0.0 0.0 0.04701488773054188 1.5861972918536398E-4 13 0.0 0.0 0.0 0.06125893941138756 1.5861972918536398E-4 14 0.0 0.0 0.0 0.07877055751345174 1.9034367502243675E-4 15 0.0 0.0 0.0 0.09786837290736956 1.9034367502243675E-4 16 0.0 0.0 0.0 0.11480895998436644 1.9034367502243675E-4 17 0.0 0.0 0.0 0.13289160911149794 1.9034367502243675E-4 18 0.0 0.0 0.0 0.15116460191365186 1.9034367502243675E-4 19 0.0 0.0 0.0 0.1710555159534965 1.9034367502243675E-4 20 0.0 3.1723945837072795E-5 0.0 0.19386503301035185 1.9034367502243675E-4 21 0.0 3.1723945837072795E-5 0.0 0.21867315865494277 2.5379156669658236E-4 22 0.0 3.1723945837072795E-5 0.0 0.24706609017912293 2.5379156669658236E-4 23 0.0 3.1723945837072795E-5 0.0 0.27352386100724163 3.1723945837072796E-4 24 0.0 3.1723945837072795E-5 0.0 0.299854736052012 3.4896340420780073E-4 25 0.0 3.1723945837072795E-5 0.0 0.33040489589311317 3.4896340420780073E-4 26 0.0 3.1723945837072795E-5 0.0 0.3619702220010006 4.441352417190191E-4 27 0.0 3.1723945837072795E-5 0.0 0.39369416783807337 4.441352417190191E-4 28 0.0 3.1723945837072795E-5 0.0 0.4244346713541969 4.441352417190191E-4 29 0.0 3.1723945837072795E-5 0.0 0.4576813665914492 4.441352417190191E-4 30 0.0 3.1723945837072795E-5 0.0 0.49413218035824585 4.758591875560919E-4 31 0.0 3.1723945837072795E-5 0.0 0.5340091802754463 4.758591875560919E-4 32 0.0 3.1723945837072795E-5 0.0 0.575726169051197 4.758591875560919E-4 33 0.0 3.1723945837072795E-5 0.0 0.6204252087356327 5.075831333931647E-4 34 0.0 3.1723945837072795E-5 0.0 0.666773893603596 5.075831333931647E-4 35 0.0 3.1723945837072795E-5 0.0 0.7166439364594744 5.075831333931647E-4 36 0.0 3.1723945837072795E-5 0.0 0.770955331732543 5.075831333931647E-4 37 0.0 3.1723945837072795E-5 0.0 0.8256474143556565 5.710310250673103E-4 38 0.0 3.1723945837072795E-5 0.0 0.88164017875809 6.662028625785286E-4 39 0.0 3.1723945837072795E-5 0.0 0.9389336249398434 6.662028625785286E-4 40 0.0 3.1723945837072795E-5 0.0 0.9989870544094223 7.296507542526743E-4 41 0.0 3.1723945837072795E-5 0.0 1.0672252619049658 7.296507542526743E-4 42 0.0 3.1723945837072795E-5 0.0 1.1416813627845757 7.296507542526743E-4 43 0.0 3.1723945837072795E-5 0.0 1.2234022472608752 7.296507542526743E-4 44 0.0 3.1723945837072795E-5 0.0 1.3093106925876683 7.296507542526743E-4 45 0.0 3.1723945837072795E-5 0.0 1.392808118030844 7.296507542526743E-4 46 0.0 3.1723945837072795E-5 0.0 1.487440648462832 7.296507542526743E-4 47 0.0 3.1723945837072795E-5 0.0 1.582453866244865 7.296507542526743E-4 48 0.0 3.1723945837072795E-5 0.0 1.6829553266567117 7.296507542526743E-4 49 0.0 3.1723945837072795E-5 0.0 1.7840278180936255 8.248225917638927E-4 50 0.0 3.1723945837072795E-5 0.0 1.8888437351393141 8.248225917638927E-4 51 0.0 3.1723945837072795E-5 0.0 2.0021616696693383 8.248225917638927E-4 52 0.0 3.1723945837072795E-5 0.0 2.125821610542248 8.565465376009654E-4 53 0.0 3.1723945837072795E-5 0.0 2.2599187295955545 8.565465376009654E-4 54 0.0 3.1723945837072795E-5 0.0 2.401248908299714 8.565465376009654E-4 55 0.0 3.1723945837072795E-5 0.0 2.552096270754995 8.565465376009654E-4 56 0.0 3.1723945837072795E-5 0.0 2.70897118291932 8.565465376009654E-4 57 0.0 3.1723945837072795E-5 0.0 2.8681936670755883 9.19994429275111E-4 58 0.0 3.1723945837072795E-5 0.0 3.0402960732417084 9.19994429275111E-4 59 0.0 3.1723945837072795E-5 0.0 3.207481267803082 9.19994429275111E-4 60 0.0 3.1723945837072795E-5 0.0 3.383263651686302 9.19994429275111E-4 61 0.0 6.344789167414559E-5 0.0 3.5726873322794637 9.19994429275111E-4 62 0.0 6.344789167414559E-5 0.0 3.765442027185518 9.19994429275111E-4 63 0.0 6.344789167414559E-5 0.0 3.9700614778346375 0.0010151662667863294 64 0.0 6.344789167414559E-5 0.0 4.183341565697278 0.0010151662667863294 65 0.0 6.344789167414559E-5 0.0 4.4140063758786345 0.0010151662667863294 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTTAC 380 0.0 24.28259 4 TTACGCG 120 0.0012201745 17.749758 56 TCGCGCT 400 0.0 17.748348 37 GCGGTTA 545 0.0 16.930712 3 CGCGGAA 345 1.0913936E-11 16.464327 59 CTCGCGC 435 0.0 16.319027 36 AGTGTAC 1370 0.0 15.803595 11 CGCTCCG 455 0.0 15.603439 25 GTAACGT 275 4.6520654E-8 15.486765 2 AATTCGC 460 0.0 15.433836 55 CTACGGT 140 0.0034221974 15.211181 35 TAAGGCG 1520 0.0 15.180473 17 CGCGCTT 480 0.0 14.788649 38 TCGCGGA 435 1.8189894E-12 14.689688 58 ATTCGCG 440 1.8189894E-12 14.522531 56 GGGCGGT 695 0.0 14.298787 1 GTTACGC 150 0.0054074596 14.199131 55 GACGCAT 280 9.560699E-7 13.9488945 48 GTACATA 1790 0.0 13.884916 47 GACCGTA 155 0.0066895154 13.750477 69 >>END_MODULE