FastQCFastQC Report
Thu 26 May 2016
SRR936159_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936159_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3152193
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88710.2814231235206727No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65010.20623737188681024No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44460.14104466319162565No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA32680.1036738549955539No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA12500.046.19495100-101
GGTATCA87900.039.7377131
CGTACAT19550.034.86515100-101
GCGTATC3350.033.763046100-101
CCGTACA8450.033.46337100-101
CGGTATC14200.032.699345100-101
TACGCAG9150.032.529827100-101
GTATCAA170250.031.4214481
TCGAGTA5150.030.631723100-101
CCCGTAC3700.030.5677998-99
GGGTATC13300.030.21238100-101
AGGTATC23350.030.210941100-101
AGTATCA33750.028.662392100-101
GCGTACA7900.028.25771100-101
CCTGTCG10950.027.99348696-97
TTACGCA4200.027.638733100-101
TTATACG6150.027.09859896-97
ACCGTAC3650.026.90935198-99
CCGCGTA1006.098344E-826.78703598-99
TGTATCA26900.026.666595100-101
Produced by FastQC (version 0.11.5)