FastQCFastQC Report
Thu 26 May 2016
SRR936158_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936158_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3154856
Sequences flagged as poor quality0
Sequence length77
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87530.27744530970668707No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGCT3850.018.44030237
CGCGGAA3700.018.23022359
AATTCGC3750.017.98715455
CGGTTAC2852.2009772E-1017.4351984
CTCGCGC4200.016.90200236
CAATTCG4150.016.2534554
TTACGCG1553.643234E-416.03192956
CGCGCTT4900.015.938738
CCCCGTA1806.353546E-515.7739521
TAACGAT1400.00341801915.21397423
ATTCGCG4301.8189894E-1214.8601656
CCGATAA2202.1488138E-514.52288922
CGCTCCG4900.014.4883525
CGATCGT4700.014.3475861
CCTCGCG4950.014.34041135
TCGCGGA4553.6379788E-1214.04455958
TAAGGCG15050.013.91621717
ACGAATA2303.264841E-513.8925648
ATACGTA2102.3574404E-413.5213879
GACGCGA9750.013.1157469
Produced by FastQC (version 0.11.5)