FastQCFastQC Report
Thu 26 May 2016
SRR936158_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936158_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3154856
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88730.28124896984204667No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63970.20276678238246057No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43810.13886529210841952No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA32820.10403010470208467No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86250.043.950081
CGTATCA15600.043.876476100-101
CGTACAT20350.033.781277100-101
CGGTATC14750.032.88699100-101
TACGCAG9550.031.785227100-101
CCGTACA7450.030.758347100-101
TTATACG6200.030.71481796-97
GTATCAA175200.030.5694581
AGGTATC22950.030.472893100-101
AGTATCA35700.030.343224100-101
TGTATCA25900.030.104403100-101
GCGTATC5000.029.164503100-101
ACGTATC5800.028.733503100-101
AACGTAT3850.028.59705498-99
GCGTACA7400.028.553223100-101
GGGTATC13050.028.505457100-101
TCGGTAT4350.028.04937100-101
TCGAGTA5000.026.783728100-101
CCTGTCG11250.026.7133696-97
TCGTATC8650.026.49129100-101
Produced by FastQC (version 0.11.5)