FastQCFastQC Report
Thu 26 May 2016
SRR936157_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936157_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3306742
Sequences flagged as poor quality0
Sequence length77
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81690.24704074282178654No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGT3250.018.5664021
CGGTTAC2559.367795E-1018.0952824
CTCGCGC3550.017.99671436
ATTCGCG4000.017.74944556
GCGTAAG6400.017.19321438
CGCGGAA3155.0931703E-1116.90559
GACGTAT4050.016.65279239
TTGCGTA6750.016.30072836
TAAGGCG12550.015.83822417
TGCGTAA6750.015.77632937
AATTCGC3602.1827873E-1115.77609155
CGTAAGT7000.015.71998539
CCTCGCG3853.6379788E-1215.67317635
CGATTCG2956.486516E-915.64286710
TCGCGCT3553.0559022E-1014.99862337
TACGGCG1500.00540158114.201488548
CGATACC1800.001126387913.81494471
GCGTTCG1550.006720595513.74088210
GCGTCAA1550.00672530813.7394273
TCGCGGA3901.2551027E-913.653625558
Produced by FastQC (version 0.11.5)