##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936157_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3306742 Sequences flagged as poor quality 0 Sequence length 77 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.80976380981643 40.0 36.0 41.0 30.0 41.0 2 36.669144432798205 39.0 36.0 41.0 30.0 41.0 3 36.58263783506545 39.0 36.0 41.0 30.0 41.0 4 36.46885151608441 39.0 36.0 41.0 30.0 41.0 5 36.39658733581271 39.0 36.0 41.0 30.0 41.0 6 36.302228598421046 39.0 36.0 41.0 30.0 41.0 7 36.13722056332185 39.0 35.0 40.0 29.0 41.0 8 35.960021979337974 39.0 35.0 40.0 29.0 41.0 9 35.86418232810422 39.0 35.0 40.0 28.0 41.0 10-11 36.08329754785828 39.0 35.0 40.5 29.0 41.0 12-13 36.269458125248356 39.0 35.0 41.0 30.0 41.0 14-15 36.216789819102914 39.0 35.0 41.0 29.5 41.0 16-17 36.057710731590184 39.0 35.0 41.0 29.0 41.0 18-19 35.90861821091576 39.0 35.0 41.0 28.0 41.0 20-21 35.71318733061122 39.0 35.0 41.0 27.5 41.0 22-23 35.46411966824143 39.0 35.0 41.0 26.5 41.0 24-25 35.245740822840126 39.0 35.0 41.0 26.0 41.0 26-27 34.30177603816688 38.0 33.5 40.0 24.0 40.5 28-29 34.33796362099008 38.0 33.5 40.0 24.0 40.5 30-31 34.71800385394445 38.0 34.0 40.0 24.5 41.0 32-33 34.56472927128878 38.0 34.0 40.0 24.0 41.0 34-35 34.29492397653037 38.0 34.0 40.0 23.0 41.0 36-37 34.00841765096884 38.0 33.0 40.0 22.5 41.0 38-39 33.73195429217036 37.0 33.0 40.0 22.0 41.0 40-41 33.3546251869665 37.0 33.0 40.0 21.5 41.0 42-43 32.998061233685604 36.0 32.0 39.0 20.0 41.0 44-45 32.62631526741427 36.0 32.0 39.0 19.0 41.0 46-47 32.23577224954351 35.0 32.0 39.0 18.0 40.0 48-49 31.772402261803308 35.0 31.0 38.0 14.0 40.0 50-51 31.340731904696526 35.0 31.0 37.0 10.0 39.0 52-53 30.893243561184995 35.0 31.0 36.5 7.5 39.0 54-55 30.430468872382544 35.0 30.0 36.0 4.5 38.5 56-57 29.987667770875383 34.0 30.0 36.0 2.0 37.0 58-59 29.56335510904691 34.0 29.5 35.0 2.0 37.0 60-61 29.21653972399419 34.0 29.0 35.0 2.0 36.5 62-63 28.879128156959325 34.0 29.0 35.0 2.0 36.0 64-65 28.513598581322643 34.0 29.0 35.0 2.0 36.0 66-67 28.137767173852694 34.0 28.5 35.0 2.0 35.0 68-69 27.79280693806774 34.0 27.5 35.0 2.0 35.0 70-71 27.380353381062086 34.0 27.0 35.0 2.0 35.0 72-73 26.908128151515903 33.0 25.5 35.0 2.0 35.0 74-75 26.338054647142112 33.0 25.0 35.0 2.0 35.0 76-77 24.625801922254595 31.0 20.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7931847854893306 1101 2 0.8082760036055348 1101 3 0.7848930298859784 1101 4 0.9062612106521897 1101 5 0.9707092980049481 1101 6 0.9418826675812113 1101 7 0.8694558500800866 1101 8 0.9790070691978272 1101 9 0.9650967556751837 1101 10-11 0.9897600513250211 1101 12-13 0.8767264152099585 1101 14-15 0.982010902477974 1101 16-17 1.0219065765093447 1101 18-19 1.0236674344138095 1101 20-21 0.909977548144731 1101 22-23 1.0343161816225503 1101 24-25 1.0756956204818522 1101 26-27 0.9827127509668045 1101 28-29 1.0434951872413123 1101 30-31 1.1327028076748533 1101 32-33 1.1681627135605268 1101 34-35 1.086940987749891 1101 36-37 1.075022817617885 1101 38-39 1.2107799136934858 1101 40-41 1.0066832737782505 1101 42-43 0.9708955932755217 1101 44-45 1.0409489106138423 1101 46-47 1.011665824020298 1101 48-49 1.137238244727314 1101 50-51 1.0742259947559134 1101 52-53 1.1252988195180649 1101 54-55 1.1410306891377644 1101 56-57 1.1016799330272349 1101 58-59 1.1349457862075738 1101 60-61 1.090796472939271 1101 62-63 1.0836156943101507 1101 64-65 0.9667916394962326 1101 66-67 0.9838847721077215 1101 68-69 0.9428623293763216 1101 70-71 0.9007978004268402 1101 72-73 0.8629980476570793 1101 74-75 0.8581223511614446 1101 76-77 0.7600049757503164 1102 1 0.8413162142944657 1102 2 0.9153111358114217 1102 3 0.8153658218534261 1102 4 0.8025562761927745 1102 5 0.9869456735391964 1102 6 1.0146347585404953 1102 7 0.8568640830405556 1102 8 0.9440379479119656 1102 9 0.8436248021364037 1102 10-11 0.7873442009334113 1102 12-13 1.0221976223427376 1102 14-15 0.9202478505976472 1102 16-17 0.9096785545874937 1102 18-19 0.9307766959831056 1102 20-21 0.9772895663038668 1102 22-23 1.122214435236998 1102 24-25 1.0492695249009856 1102 26-27 0.86163138248763 1102 28-29 0.9335828132612249 1102 30-31 1.0342666816242598 1102 32-33 0.9847416178774111 1102 34-35 1.027070790821405 1102 36-37 1.0696663320654451 1102 38-39 1.019434133443589 1102 40-41 1.0998743639400104 1102 42-43 1.0506419174815704 1102 44-45 1.0473145779826112 1102 46-47 0.8628304218812843 1102 48-49 0.9732924665223663 1102 50-51 1.0760978889912067 1102 52-53 1.1422713763164936 1102 54-55 1.0414575720235923 1102 56-57 1.0542803399718785 1102 58-59 0.9736441257098356 1102 60-61 0.8281750541543786 1102 62-63 0.9021702739710982 1102 64-65 0.9495376467353474 1102 66-67 1.011581547224793 1102 68-69 0.9305003633633611 1102 70-71 1.0891847966027868 1102 72-73 1.0219083971386844 1102 74-75 1.0511748960991731 1102 76-77 1.197440304271204 1103 1 0.7616710640664479 1103 2 0.4708838748528166 1103 3 0.6689885532542448 1103 4 0.7748518064819052 1103 5 0.7086488420524617 1103 6 0.6672073325045815 1103 7 0.7356324325587948 1103 8 0.7177130747480618 1103 9 0.7310420782177189 1103 10-11 0.7039059012603488 1103 12-13 0.6762653469692594 1103 14-15 0.7332880379782765 1103 16-17 0.8553129753015796 1103 18-19 0.8919223529422382 1103 20-21 0.9306582933022867 1103 22-23 0.8277333325001379 1103 24-25 0.8081113057120533 1103 26-27 0.5868582104632765 1103 28-29 0.6724450268610411 1103 30-31 0.8820331549852227 1103 32-33 0.9194278355576486 1103 34-35 1.011681446024987 1103 36-37 0.9411707178577515 1103 38-39 0.9093062573037045 1103 40-41 0.9187757156829548 1103 42-43 0.9574513819820325 1103 44-45 0.8876128108376946 1103 46-47 0.7510601031900848 1103 48-49 0.8841752519234021 1103 50-51 1.0470398875714437 1103 52-53 0.9416420893533584 1103 54-55 0.9913661662139965 1103 56-57 1.0491318189470071 1103 58-59 0.8881214056037763 1103 60-61 0.9397917436235197 1103 62-63 0.9388906733866342 1103 64-65 0.8994098523675014 1103 66-67 0.8816594708691312 1103 68-69 0.8151338125233814 1103 70-71 0.8385411739787116 1103 72-73 0.8918553833023459 1103 74-75 0.8488799126605677 1103 76-77 0.932769863484129 1104 1 0.4973066581916754 1104 2 0.43514791108322726 1104 3 0.4277968710719193 1104 4 0.33463541330306157 1104 5 0.427749319466308 1104 6 0.2413431528463974 1104 7 0.5710584969811094 1104 8 0.5712337846162256 1104 9 0.46833980557284605 1104 10-11 0.4396425603169334 1104 12-13 0.5966837408860926 1104 14-15 0.5892873963277054 1104 16-17 0.6121548228380576 1104 18-19 0.5548589373051271 1104 20-21 0.5406826929095487 1104 22-23 0.6658045169761664 1104 24-25 0.6942669000160677 1104 26-27 0.5127268598235872 1104 28-29 0.6059005486068898 1104 30-31 0.7238584422753291 1104 32-33 0.7114048692348689 1104 34-35 0.839096314340118 1104 36-37 0.7299169515129265 1104 38-39 0.8037294596267586 1104 40-41 0.8330899367355471 1104 42-43 0.8596149661269052 1104 44-45 0.8288970158539897 1104 46-47 0.7228535513020589 1104 48-49 0.8274099852833245 1104 50-51 0.7325047639072011 1104 52-53 0.8034862288537532 1104 54-55 0.8882084209900007 1104 56-57 0.7732294729990166 1104 58-59 0.7039264213048462 1104 60-61 0.5610794449401446 1104 62-63 0.608138526812187 1104 64-65 0.6323031849128391 1104 66-67 0.6278865208110957 1104 68-69 0.5159404944300796 1104 70-71 0.4620756952264351 1104 72-73 0.4640815482432892 1104 74-75 0.5361808433022155 1104 76-77 0.48692656230119624 1105 1 0.24043688022445053 1105 2 0.2865283817636026 1105 3 0.18536147612942955 1105 4 0.262541124747095 1105 5 0.1627294449857004 1105 6 -0.04636585841985408 1105 7 0.05041641437119182 1105 8 0.3039980874845938 1105 9 0.11384630159967912 1105 10-11 0.24916255782179775 1105 12-13 0.3247769343529825 1105 14-15 0.04513208774476851 1105 16-17 0.13602637859229816 1105 18-19 0.2280483149410628 1105 20-21 0.16544591455073032 1105 22-23 0.1867626506116835 1105 24-25 0.314997352156162 1105 26-27 0.19092523035876496 1105 28-29 0.33195777740773025 1105 30-31 0.2793333678543277 1105 32-33 0.3692789409216246 1105 34-35 0.3739641585740898 1105 36-37 0.47153137619262964 1105 38-39 0.32808029012706896 1105 40-41 0.44420023923657936 1105 42-43 0.30203130097464737 1105 44-45 0.09151670458531669 1105 46-47 0.07930331409051661 1105 48-49 0.06241852767124101 1105 50-51 0.22266871537673083 1105 52-53 0.13903954340423041 1105 54-55 0.063930365396903 1105 56-57 0.19844736453667977 1105 58-59 0.19964760330795528 1105 60-61 -0.04499206160439684 1105 62-63 -0.034086513993742074 1105 64-65 0.045337170794507387 1105 66-67 0.10688194985383248 1105 68-69 -0.1705871375090986 1105 70-71 -0.3887717858954076 1105 72-73 -0.31000781241182196 1105 74-75 -0.2875316472976941 1105 76-77 -0.09454945143593108 1106 1 0.1404684160175762 1106 2 0.22345679551320785 1106 3 0.020744636015898266 1106 4 0.19883882263419395 1106 5 -0.10343264899096738 1106 6 0.13528030998128315 1106 7 0.38122688425450946 1106 8 0.36170858890370283 1106 9 0.13371385126855273 1106 10-11 0.10015593530523859 1106 12-13 0.13587753353305487 1106 14-15 0.1320131978046888 1106 16-17 0.19657510139267487 1106 18-19 0.2952195542977307 1106 20-21 0.2348246594261596 1106 22-23 0.28215842481540676 1106 24-25 0.2806233376497005 1106 26-27 0.24642822625911265 1106 28-29 0.3106711170482299 1106 30-31 0.3523387289391593 1106 32-33 0.3309629522745041 1106 34-35 0.2706844360890699 1106 36-37 0.3758202440576568 1106 38-39 0.2544442131797311 1106 40-41 0.39752726541128425 1106 42-43 0.33340941513421996 1106 44-45 0.13992414703249523 1106 46-47 0.3524032825547181 1106 48-49 0.28443051127263175 1106 50-51 0.018159096959827536 1106 52-53 0.15922245100435362 1106 54-55 0.20347624997589975 1106 56-57 0.18756751590848708 1106 58-59 0.1504517660326492 1106 60-61 -0.03726579228872495 1106 62-63 -0.12459424026305754 1106 64-65 0.04975218183203722 1106 66-67 0.03088068842210845 1106 68-69 3.368612294707418E-4 1106 70-71 -0.21169830749742502 1106 72-73 -0.20120932612988796 1106 74-75 -0.35170698630746955 1106 76-77 -0.1785923401145837 1107 1 0.18690823605150797 1107 2 0.14903058089378618 1107 3 0.14433948017999398 1107 4 0.08392246994570485 1107 5 0.28198015376644747 1107 6 -0.0019697165728800314 1107 7 -0.021098282596170748 1107 8 0.14597645937205783 1107 9 0.03167856777997713 1107 10-11 0.10758567908684569 1107 12-13 0.23817211347231648 1107 14-15 0.19412065246145715 1107 16-17 0.20630012225112893 1107 18-19 0.2624510863727849 1107 20-21 0.11033617202397039 1107 22-23 0.0653835904751503 1107 24-25 -0.16191562967846806 1107 26-27 -0.1731766090484328 1107 28-29 0.06367110823718036 1107 30-31 0.11671358485195071 1107 32-33 0.2779747833770472 1107 34-35 0.21164012650847752 1107 36-37 0.15628493854146797 1107 38-39 0.22891067781205265 1107 40-41 0.2655252324806412 1107 42-43 0.2701821348238198 1107 44-45 0.367797266443354 1107 46-47 0.3039150399252861 1107 48-49 0.35755888809330827 1107 50-51 0.276644592673545 1107 52-53 0.37129954628766626 1107 54-55 0.38521643512595816 1107 56-57 0.4016046812812384 1107 58-59 0.3460139140568437 1107 60-61 0.44630243283868865 1107 62-63 0.39320987898735993 1107 64-65 0.44179887804378026 1107 66-67 0.532851554461601 1107 68-69 0.4308160745716769 1107 70-71 0.3192994793812254 1107 72-73 0.3946681991904839 1107 74-75 0.42984824436906166 1107 76-77 0.5339231772481625 1108 1 -0.03373609192187388 1108 2 0.06959090734985551 1108 3 0.044246717023263216 1108 4 -0.055683138587042436 1108 5 0.08650368171061018 1108 6 0.08621207418104859 1108 7 -0.08126197580092764 1108 8 -0.003253408402848379 1108 9 0.06928047905034873 1108 10-11 -0.030559687346304543 1108 12-13 -0.04173858692733745 1108 14-15 0.04710132389259769 1108 16-17 0.1163691794753774 1108 18-19 0.010078227988806532 1108 20-21 -0.15171302037798995 1108 22-23 -0.04967074616740064 1108 24-25 0.038603922461653895 1108 26-27 -0.10326769366933775 1108 28-29 0.012776071276235257 1108 30-31 0.0928244799318847 1108 32-33 0.06065721626199405 1108 34-35 0.029527769972141016 1108 36-37 0.02709951600393623 1108 38-39 0.022402993489997414 1108 40-41 -0.03369563546529264 1108 42-43 0.0534516349125056 1108 44-45 -0.07703625727038599 1108 46-47 -0.08869462931909311 1108 48-49 -0.09287409848895933 1108 50-51 -0.17228485735424215 1108 52-53 -0.11395988277289604 1108 54-55 -0.1085506111899548 1108 56-57 -0.21662939254762392 1108 58-59 -0.2545909099448913 1108 60-61 -0.24367129999053105 1108 62-63 -0.25724998098146656 1108 64-65 -0.3256380220396231 1108 66-67 -0.4292702343812067 1108 68-69 -0.46031074791704185 1108 70-71 -0.44137923569166304 1108 72-73 -0.3550759338504328 1108 74-75 -0.4840891513821397 1108 76-77 -0.26282864576597476 1109 1 0.0349634602100366 1109 2 -0.1738769624995129 1109 3 -0.2684205636750079 1109 4 0.1320028357441032 1109 5 -0.051985971565493116 1109 6 -0.10510260270081062 1109 7 -0.0020821127437784526 1109 8 0.03774745297695148 1109 9 0.07953551625994493 1109 10-11 -0.11097969598480972 1109 12-13 0.014886208535337175 1109 14-15 0.14348968551706065 1109 16-17 0.2031059258084369 1109 18-19 -0.06338456631358724 1109 20-21 0.09005783815334922 1109 22-23 -0.008579575775911508 1109 24-25 -0.09202105580433084 1109 26-27 -0.10330864388925676 1109 28-29 -0.02719856429055767 1109 30-31 0.09915988750628912 1109 32-33 0.13691977995099336 1109 34-35 0.11392185018555523 1109 36-37 0.23133997303498433 1109 38-39 0.11326412055786506 1109 40-41 0.13698207832288034 1109 42-43 0.13785987193048044 1109 44-45 0.0027829897890399025 1109 46-47 -0.08453552165764222 1109 48-49 0.03210112710304713 1109 50-51 0.0742940890640611 1109 52-53 0.18194004059033375 1109 54-55 0.2824231337333103 1109 56-57 0.3496834553510979 1109 58-59 0.36280386936762454 1109 60-61 0.3988258691236162 1109 62-63 0.3821843302101833 1109 64-65 0.4386089965278366 1109 66-67 0.4860286062620105 1109 68-69 0.434276022558592 1109 70-71 0.5254654329982564 1109 72-73 0.4798492640645442 1109 74-75 0.5256599463353453 1109 76-77 0.69429979939693 1110 1 -0.44267555256512736 1110 2 -0.3874106412867704 1110 3 -0.5846986256175626 1110 4 -0.436356453671479 1110 5 -0.32123857899500763 1110 6 -0.49591215172672776 1110 7 -0.6746071249797438 1110 8 -0.6657208014805391 1110 9 -0.4441643710726595 1110 10-11 -0.484179765649948 1110 12-13 -0.4318696277768339 1110 14-15 -0.5191623541935613 1110 16-17 -0.5149198733168134 1110 18-19 -0.41807611982390824 1110 20-21 -0.6102797555473813 1110 22-23 -0.7563231642435824 1110 24-25 -0.6160819875495704 1110 26-27 -0.5783530387049183 1110 28-29 -0.5089630827507108 1110 30-31 -0.573397524961095 1110 32-33 -0.5436962849192852 1110 34-35 -0.427673734024971 1110 36-37 -0.45254062403535755 1110 38-39 -0.36501630539469687 1110 40-41 -0.3616762364491706 1110 42-43 -0.412701014138797 1110 44-45 -0.4196230049779004 1110 46-47 -0.4557941768827867 1110 48-49 -0.3428903318617387 1110 50-51 -0.4529137499723319 1110 52-53 -0.5307477043609836 1110 54-55 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-0.5640391360075867 2314 12-13 -0.5720967395897532 2314 14-15 -0.541879456182734 2314 16-17 -1.1256039338143538 2314 18-19 -0.711981553517326 2314 20-21 -0.5941570036439856 2314 22-23 -0.49722043752392153 2314 24-25 -0.45374499127792234 2314 26-27 -0.4843382028205099 2314 28-29 -0.6355082310695721 2314 30-31 -0.7189374249274536 2314 32-33 -0.7360458361457773 2314 34-35 -0.7364321221926815 2314 36-37 -0.8382415546767703 2314 38-39 -0.7978876653958835 2314 40-41 -0.670970255529511 2314 42-43 -0.5519935015269439 2314 44-45 -0.5154298899650414 2314 46-47 -0.5033177720165583 2314 48-49 -0.5398380353384304 2314 50-51 -0.4307691102490949 2314 52-53 -0.4122025720356959 2314 54-55 -0.3069070281901922 2314 56-57 -0.15435720017556775 2314 58-59 -0.3361039200927287 2314 60-61 -0.40134534112295484 2314 62-63 -0.22805232025368838 2314 64-65 -0.1915485970351618 2314 66-67 -0.21845074623786687 2314 68-69 -0.11931777697112622 2314 70-71 0.020948726710507515 2314 72-73 0.14143436688278754 2314 74-75 0.0822465727098951 2314 76-77 0.05877009457263682 2315 1 -1.2008139340835413 2315 2 -1.0928579555634457 2315 3 -1.069168335518036 2315 4 -1.0857878293670993 2315 5 -0.9985819329372347 2315 6 -1.143180997157458 2315 7 -1.0806304174238974 2315 8 -1.1043922950038763 2315 9 -1.0966568521065554 2315 10-11 -1.1871052711761934 2315 12-13 -1.090745174251822 2315 14-15 -1.537510601565394 2315 16-17 -1.3422055050168495 2315 18-19 -1.251888464551321 2315 20-21 -1.3697834670947344 2315 22-23 -1.357488193226736 2315 24-25 -1.3315726290010517 2315 26-27 -1.3442115408075495 2315 28-29 -1.2658363924389562 2315 30-31 -1.4630739055332498 2315 32-33 -1.3586117138983482 2315 34-35 -1.4014406219881508 2315 36-37 -1.6566322193514722 2315 38-39 -1.5200532469916297 2315 40-41 -1.4290756679843888 2315 42-43 -1.6514240006558616 2315 44-45 -1.6596945719441578 2315 46-47 -1.6454969260790477 2315 48-49 -1.4987993682440575 2315 50-51 -1.3787674281959994 2315 52-53 -1.531414337634839 2315 54-55 -1.3676301097536658 2315 56-57 -1.6094286638352102 2315 58-59 -1.5093065988851286 2315 60-61 -1.5749704417747736 2315 62-63 -1.7678126866797506 2315 64-65 -1.8452558806526618 2315 66-67 -1.7263589845364038 2315 68-69 -1.773708715649196 2315 70-71 -1.933986811012776 2315 72-73 -3.288161723835927 2315 74-75 -3.8361632333348616 2315 76-77 -3.3751540417511166 2316 1 -3.1674203395492597 2316 2 -3.182450300809407 2316 3 -2.5260478469278524 2316 4 -2.458743420406776 2316 5 -3.265196845866612 2316 6 -2.6419124852437363 2316 7 -3.0097815688763845 2316 8 -3.008199630400817 2316 9 -2.4759460044359614 2316 10-11 -2.6695985470078014 2316 12-13 -2.6770517570654278 2316 14-15 -2.779439390822425 2316 16-17 -3.7864119631633955 2316 18-19 -3.6429619265000497 2316 20-21 -3.0233699356465635 2316 22-23 -3.2428198943268143 2316 24-25 -3.29521361076236 2316 26-27 -3.0595263660446186 2316 28-29 -3.146519091857172 2316 30-31 -3.3924534905653445 2316 32-33 -3.6204390925524734 2316 34-35 -3.6546594290454166 2316 36-37 -3.4678974509835356 2316 38-39 -3.482508910017536 2316 40-41 -3.335113963023229 2316 42-43 -3.548664842044019 2316 44-45 -3.3684868720351204 2316 46-47 -3.045487232478621 2316 48-49 -3.1206442035591913 2316 50-51 -3.502064912931644 2316 52-53 -4.485424011602653 2316 54-55 -3.682977492399747 2316 56-57 -3.6797379428603847 2316 58-59 -3.8459761804487975 2316 60-61 -3.6993297234176303 2316 62-63 -3.551321786393771 2316 64-65 -3.4607264988180617 2316 66-67 -3.4935851260089805 2316 68-69 -3.5062172476536233 2316 70-71 -3.4608435240560276 2316 72-73 -3.4532627581471793 2316 74-75 -3.363018433031211 2316 76-77 -3.250154409862528 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 70754.0 3 6549.0 4 8290.0 5 10064.0 6 11508.0 7 12681.0 8 14479.0 9 15118.0 10 18130.0 11 20086.0 12 16072.0 13 14373.0 14 14185.0 15 14918.0 16 15098.0 17 15967.0 18 17139.0 19 18362.0 20 19525.0 21 20815.0 22 23311.0 23 26542.0 24 31022.0 25 36745.0 26 43158.0 27 50962.0 28 60636.0 29 73020.0 30 86708.0 31 103752.0 32 127110.0 33 161128.0 34 216103.0 35 290846.0 36 412132.0 37 558327.0 38 511859.0 39 138295.0 40 973.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.59069695559219 26.73647624638469 28.55657004563542 22.1162567523877 2 22.197330574987976 26.963823937719468 28.60818490592288 22.230660581369676 3 22.3222268329597 27.09219270409268 28.0904711380604 22.495109324887217 4 22.261407255528997 27.095674224370747 28.444845838988027 22.198072681112233 5 22.29899366401208 27.165083198970706 28.337705222464283 22.19821791455293 6 22.44110193673968 26.934131131645493 28.231027212709286 22.39373971890554 7 22.260812807866873 26.78543883071231 28.402664313121516 22.551084048299295 8 22.28634807868435 27.052464300102958 28.091309224081506 22.569878397131184 9 22.160546347695657 26.88591915789041 28.446908253137387 22.506626241276546 10-11 22.696631592479026 26.989772654232535 28.077977367507668 22.235618385780775 12-13 22.815459907117393 26.643128179899893 28.05490807997601 22.4865038330067 14-15 22.35883337643072 26.98191270737782 28.20011704342856 22.459136872762905 16-17 22.606982173556762 26.821215837010538 28.28492973202495 22.28687225740775 18-19 22.53227159742517 26.736970828375412 28.026715601613716 22.704041972585703 20-21 22.4753077970721 26.955998309110612 28.11100582963609 22.4576880641812 22-23 22.68754240346227 27.080730142626873 27.64807069729062 22.583656756620233 24-25 22.612564885221424 27.27613417090967 27.486168481454055 22.625132462414854 26-27 22.70641428276505 27.270317012470723 27.47578035613142 22.5474883486328 28-29 22.931971596852165 27.088087593258596 27.476795834342088 22.503144975547144 30-31 22.547471094081146 27.15099035690593 27.628661412607652 22.67287713640527 32-33 22.606847985560137 27.43954684289846 27.405612685446997 22.547992486094408 34-35 22.554879534278754 27.026268046494096 27.614468559112897 22.804383860114257 36-37 22.47108535072764 27.240592323482343 27.83172416629844 22.456598159491577 38-39 22.488913152460505 27.191687340691544 27.855332991055665 22.464066515792283 40-41 22.322086798302607 27.158695202119908 27.7460166620972 22.77320133748028 42-43 22.305337333021196 27.37080711789582 27.583933256267358 22.739922292815624 44-45 22.567733324340384 27.018170044492418 27.772080730279995 22.642015900887202 46-47 22.455662052300653 27.035428766402088 27.727939089098165 22.780970092199095 48-49 22.471773314758792 27.06618972984688 27.680459388706787 22.781577566687545 50-51 22.375218687027743 27.363733363827752 27.51103229352814 22.750015655616373 52-53 22.459698907893312 27.062938364049984 27.690593015884158 22.78676971217255 54-55 22.390210155324304 27.111876030955358 27.66765795680492 22.83025585691542 56-57 22.41299806431308 27.347540268080312 27.52407963372732 22.715382033879287 58-59 22.49336776158967 27.291143092112723 27.473830220141014 22.741658926156592 60-61 22.40811886348131 27.237465777052783 27.45601775638419 22.89839760308172 62-63 22.439347687894102 27.1662858039098 27.52891332890586 22.865453179290242 64-65 22.321178881087807 27.115922671736016 27.513108837418653 23.04978960975752 66-67 22.25266024302084 27.228026318535836 27.638304353520944 22.88100908492238 68-69 22.332831416883817 26.97032460591693 27.755253558569475 22.941590418629783 70-71 22.16400030984446 26.824405939815133 27.8799951828869 23.1315985674535 72-73 22.269510372419155 26.587855773462422 27.874745222100948 23.267888632017478 74-75 22.114977166075032 26.72350126639902 27.715221244509447 23.4463003230165 76-77 21.99372160579421 26.505935295187744 28.117743955206738 23.38259914381131 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 8184.0 1 4895.0 2 1528.0 3 1458.0 4 1466.0 5 1600.0 6 1767.0 7 1892.0 8 2018.0 9 2052.0 10 2110.0 11 2301.5 12 2545.0 13 2655.0 14 2772.0 15 3198.5 16 3713.0 17 3918.0 18 4318.5 19 5270.5 20 6628.5 21 8716.0 22 9997.0 23 11944.5 24 16107.5 25 21829.5 26 25336.0 27 28145.0 28 37766.5 29 47685.5 30 50792.0 31 58331.5 32 72471.0 33 85476.0 34 98199.5 35 104518.0 36 112238.5 37 125752.0 38 132149.0 39 132753.0 40 136809.0 41 144085.5 42 150290.5 43 153275.0 44 152929.0 45 153578.0 46 155755.0 47 156294.0 48 155651.0 49 154870.0 50 152446.5 51 146443.0 52 142082.0 53 139857.0 54 130981.0 55 115895.5 56 100564.5 57 93668.0 58 89464.5 59 76917.5 60 62627.5 61 56681.0 62 49725.5 63 36400.0 64 25907.0 65 21784.0 66 18476.5 67 13493.5 68 9939.5 69 6813.0 70 5565.0 71 4778.0 72 3401.5 73 2521.0 74 2230.0 75 1950.0 76 1384.5 77 909.5 78 720.0 79 583.5 80 368.0 81 239.5 82 158.0 83 126.0 84 121.5 85 108.5 86 82.5 87 65.0 88 67.5 89 56.5 90 51.5 91 60.0 92 50.0 93 36.5 94 41.0 95 46.0 96 43.0 97 48.0 98 51.5 99 115.0 100 180.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00964695763987635 2 0.012459393566235285 3 0.013608560933994852 4 0.01484845204131438 5 0.011824327389315525 6 0.009344545174676464 7 0.015846413176474006 8 0.017237510516393478 9 0.01720726926987349 10-11 0.01979289584733251 12-13 0.015891775046253987 14-15 0.00837682528603683 16-17 0.006698436104177465 18-19 0.017660887967673317 20-21 0.023875464127530967 22-23 0.021259596303551953 24-25 0.018613487233052957 26-27 0.02417787659273085 28-29 0.008391945909296823 30-31 0.008543152141896767 32-33 0.01025178257027612 34-35 0.011688241779975577 36-37 0.011279984951955733 38-39 0.01389585277593474 40-41 0.011355588068255703 42-43 0.014636763315674463 44-45 0.013563199064214868 46-47 0.024147635346210863 48-49 0.023694016648411035 50-51 0.03686407950786605 52-53 0.04056863220656465 54-55 0.02869894294746914 56-57 0.020579168256852215 58-59 0.016420996860353785 60-61 0.02006506706601241 62-63 0.02632500509565004 64-65 0.02605283387697014 66-67 0.028275565496189304 68-69 0.04267039883970385 70-71 0.056188236034138736 72-73 0.08721575496364699 74-75 0.08727623745668697 76-77 0.09166121820208532 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 3306742.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.345780188127776 #Duplication Level Percentage of deduplicated Percentage of total 1 72.87397019577556 37.41770855108466 2 13.784480743354315 14.155498365115015 3 5.358042020317715 8.253385434419563 4 2.6498957578166324 5.442438604092203 5 1.4983376654020246 3.8466658207662445 6 0.8855574312857089 2.728178232645545 7 0.5928933327263664 2.130979951602215 8 0.43252789620830073 1.77667858271558 9 0.32613046571669296 1.5070880884806952 >10 1.4538569404810893 13.095462201001457 >50 0.09363302777021003 3.346853664255467 >100 0.04695763964715111 4.1913016277071415 >500 0.002359926032832974 0.8079159320453536 >1k 0.0012979593147404856 1.0523809307360454 >5k 5.899815067002207E-5 0.247464013332837 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8169 0.24704074282178654 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 9.072373955996567E-5 0.0 3 0.0 0.0 0.0 2.4192997215990845E-4 0.0 4 0.0 0.0 0.0 8.769961490796681E-4 0.0 5 0.0 0.0 0.0 0.0020261635168392334 0.0 6 0.0 0.0 0.0 0.004566428224518272 0.0 7 0.0 0.0 0.0 0.010130817584196166 0.0 8 0.0 0.0 0.0 0.015634724450834084 0.0 9 0.0 0.0 0.0 0.020866460098792103 0.0 10 0.0 0.0 0.0 0.02724736311450969 0.0 11 0.0 0.0 0.0 0.035503223414466566 0.0 12 0.0 0.0 0.0 0.04533162853346285 0.0 13 0.0 0.0 0.0 0.05948453190481749 0.0 14 0.0 0.0 0.0 0.07660107743513102 0.0 15 0.0 0.0 0.0 0.09371762296544454 0.0 16 0.0 0.0 0.0 0.1120438183565576 0.0 17 0.0 0.0 0.0 0.13088411493851046 0.0 18 0.0 0.0 0.0 0.1485752441527038 0.0 19 0.0 0.0 0.0 0.16759698821377658 0.0 20 0.0 0.0 0.0 0.18982430440596818 0.0 21 0.0 0.0 0.0 0.21250523929595957 0.0 22 0.0 0.0 0.0 0.2386336762892297 0.0 23 0.0 0.0 0.0 0.26442945957077996 0.0 24 0.0 0.0 0.0 0.2899530716336503 0.0 25 0.0 0.0 0.0 0.31777501843203976 0.0 26 0.0 0.0 0.0 0.348772296115028 0.0 27 0.0 0.0 0.0 0.37983005629105626 0.0 28 0.0 0.0 0.0 0.4084080342524455 0.0 29 0.0 0.0 0.0 0.43964724190759363 0.0 30 0.0 0.0 0.0 0.47143079200010163 3.0241246519988556E-5 31 0.0 0.0 0.0 0.5076295640845279 3.0241246519988556E-5 32 0.0 0.0 0.0 0.546882702067473 3.0241246519988556E-5 33 0.0 0.0 0.0 0.586468493762138 6.048249303997711E-5 34 0.0 0.0 0.0 0.6306509549278414 6.048249303997711E-5 35 0.0 0.0 0.0 0.6766781321312639 6.048249303997711E-5 36 0.0 0.0 0.0 0.7270902900800849 6.048249303997711E-5 37 0.0 0.0 0.0 0.7777746192475857 6.048249303997711E-5 38 0.0 0.0 0.0 0.8261908549260873 6.048249303997711E-5 39 0.0 0.0 0.0 0.8765727716283883 6.048249303997711E-5 40 0.0 0.0 0.0 0.9300392954757281 6.048249303997711E-5 41 0.0 0.0 0.0 0.9902496172970253 6.048249303997711E-5 42 0.0 0.0 0.0 1.0552078148219608 6.048249303997711E-5 43 0.0 0.0 0.0 1.1246417168318545 9.072373955996567E-5 44 0.0 0.0 0.0 1.1987025295593063 9.072373955996567E-5 45 0.0 0.0 0.0 1.276755186827397 9.072373955996567E-5 46 0.0 0.0 0.0 1.3632753931210841 9.072373955996567E-5 47 0.0 0.0 0.0 1.4514588679733709 9.072373955996567E-5 48 0.0 0.0 0.0 1.5389165529091777 9.072373955996567E-5 49 0.0 0.0 0.0 1.6322712809163824 9.072373955996567E-5 50 0.0 0.0 0.0 1.7292851997525056 9.072373955996567E-5 51 0.0 0.0 0.0 1.8292627607475878 9.072373955996567E-5 52 0.0 0.0 0.0 1.9328995125715887 9.072373955996567E-5 53 0.0 0.0 0.0 2.048179144305785 9.072373955996567E-5 54 0.0 0.0 0.0 2.1690231653996594 9.072373955996567E-5 55 0.0 0.0 0.0 2.301328618924609 9.072373955996567E-5 56 0.0 0.0 0.0 2.4450350223875947 9.072373955996567E-5 57 0.0 0.0 0.0 2.586685021087221 9.072373955996567E-5 58 3.0241246519988556E-5 0.0 0.0 2.739887175957483 9.072373955996567E-5 59 3.0241246519988556E-5 0.0 0.0 2.893331260799905 9.072373955996567E-5 60 3.0241246519988556E-5 0.0 0.0 3.047591859298367 9.072373955996567E-5 61 3.0241246519988556E-5 0.0 0.0 3.2144025751026235 9.072373955996567E-5 62 3.0241246519988556E-5 0.0 0.0 3.387987330127358 9.072373955996567E-5 63 3.0241246519988556E-5 0.0 0.0 3.5745153386626476 1.5120623259994278E-4 64 3.0241246519988556E-5 0.0 0.0 3.7645513317942556 1.5120623259994278E-4 65 3.0241246519988556E-5 0.0 0.0 3.9706756680744975 1.8144747911993134E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGATCGT 325 0.0 18.566402 1 CGGTTAC 255 9.367795E-10 18.095282 4 CTCGCGC 355 0.0 17.996714 36 ATTCGCG 400 0.0 17.749445 56 GCGTAAG 640 0.0 17.193214 38 CGCGGAA 315 5.0931703E-11 16.905 59 GACGTAT 405 0.0 16.652792 39 TTGCGTA 675 0.0 16.300728 36 TAAGGCG 1255 0.0 15.838224 17 TGCGTAA 675 0.0 15.776329 37 AATTCGC 360 2.1827873E-11 15.776091 55 CGTAAGT 700 0.0 15.719985 39 CCTCGCG 385 3.6379788E-12 15.673176 35 CGATTCG 295 6.486516E-9 15.642867 10 TCGCGCT 355 3.0559022E-10 14.998623 37 TACGGCG 150 0.005401581 14.2014885 48 CGATACC 180 0.0011263879 13.814944 71 GCGTTCG 155 0.0067205955 13.740882 10 GCGTCAA 155 0.006725308 13.739427 3 TCGCGGA 390 1.2551027E-9 13.6536255 58 >>END_MODULE