FastQCFastQC Report
Thu 26 May 2016
SRR936157_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936157_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3306742
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79760.2412041822434287No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55470.1677481944463765No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39560.11963437123307472No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA12250.046.4051100-101
GGTATCA80450.039.2012981
CGTACAT17500.037.755775100-101
TCGAGTA5450.035.49648100-101
CGGTATC10050.033.168087100-101
CCTGTCG10050.032.8654896-97
GCGTATC3300.031.56622100-101
GTATCAA159250.030.9012971
CGAGTAC14750.030.468662100-101
TACGCAG11500.030.28004100-101
TCGTATC5650.030.02582100-101
GCGTACA7150.029.138046100-101
TCGTACA10300.027.161833100-101
TAAATCG2850.027.14635596-97
AGGTATC24650.026.321342100-101
CCGTACA8850.026.231297100-101
ACGTATC5900.026.231297100-101
CGTCCTA4200.025.510658100-101
AGTATCA40150.025.500069100-101
CTACGCG851.397674E-524.5054296-97
Produced by FastQC (version 0.11.5)