FastQCFastQC Report
Thu 26 May 2016
SRR936156_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936156_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3315751
Sequences flagged as poor quality0
Sequence length77
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82750.2495663878258651No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAG5300.020.0940438
GACGTAT5550.019.82883539
TTGCGTA5400.019.71806336
TTAGTCG2900.019.58532515
TGCGTAA5850.018.2026637
CGCCGTA1803.185387E-617.7580467
CGTAAGT5850.017.59829339
CGGTTAC2702.0081643E-917.0895024
ACGTATT7100.016.49900840
CGCGGAA2905.164111E-915.91451759
CGTATCC2502.639863E-715.629270
CCGTATC2754.6027708E-815.50026769
TAAGGCG14200.015.49789917
GACGCGA6450.015.42016169
CGTATTA7150.015.39161141
CGATCGT3101.2507371E-814.8841811
TGAGACG7900.014.82598736
CGATTGC2402.9641687E-614.79926168
AATTCGC3151.5417754E-814.64991355
TAGTCGG3654.656613E-1014.58750316
Produced by FastQC (version 0.11.5)