FastQCFastQC Report
Thu 26 May 2016
SRR936156_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936156_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3315751
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80500.24278059480340955No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56890.171575006687776No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39920.1203950477584113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA11250.043.91862100-101
CGTACAT20100.038.9451100-101
CGGTATC9950.038.887802100-101
GGTATCA81800.036.5163041
TCGAGTA5950.035.016617100-101
GTATCAA163500.033.117691
CGAGTAC13600.033.046932100-101
CCTGTCG9300.031.9964796-97
CCGTACA10600.029.202538100-101
TACGCAG11750.028.877535100-101
TAAATCG2550.028.0063296-97
TCGTATC5950.027.513058100-101
TCGTACA10250.027.29588100-101
ACCGTAC3600.027.27987198-99
GCGTACA7450.026.767736100-101
AGTATCA38050.026.75251100-101
CCGGTAT4300.026.303179100-101
TATCAAC208350.025.785272
CGACGCA1850.025.741945100-101
ATCAACG207050.025.7169063
Produced by FastQC (version 0.11.5)