FastQCFastQC Report
Thu 26 May 2016
SRR936155_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936155_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409411
Sequences flagged as poor quality0
Sequence length77
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5330313.019435237450875No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4233110.339487703066112No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA15390.3759058745368346No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC14450.3529460615371839No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9780.23887975652828086No Hit
AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG9180.2242245567412698No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG7350.17952619739088593No Hit
AGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGC7250.17708366409305074No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6180.1509485578062143No Hit
CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG5580.1362933580192032No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT5560.13580485135963616No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC5260.12847725146613062No Hit
TACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT4860.11870711827478989No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG4660.11382205167911952No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4200.10258639850907766No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGAT200.00656812653.2533918
TCATCGG350.001169910240.57401321
ATCGGAG350.001169910240.57401323
CGGTTAC601.1044351E-535.489254
GAGGTGC500.00672085328.40180813
CGAGGTG500.0067288828.39486912
GCGGTTA755.075808E-528.39143
GTGGGTA755.075808E-528.39141
GCCTAGT807.857598E-526.62669617
GGCGGTT807.8772246E-526.6169382
CACGGGG750.001685347423.66817340
CTGCCTA1051.8256447E-523.66528115
CTAGTGG952.5219622E-422.42248219
GAGTACT1604.2229658E-822.1807826
GGTCACT800.002457923322.18078234
GTTACAA1153.6938312E-521.602156
ATTTGCG1505.5694363E-721.2935493
GGTTACA1205.1277988E-520.7020635
CCTAGTG1054.9584545E-420.28700618
TGCCTAG1159.146572E-418.52291716
Produced by FastQC (version 0.11.5)