##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936155_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 409411 Sequences flagged as poor quality 0 Sequence length 77 %GC 30 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.852842253872026 40.0 35.0 41.0 31.0 41.0 2 36.64039315015962 39.0 35.0 41.0 31.0 41.0 3 36.50109059111748 39.0 35.0 41.0 31.0 41.0 4 36.320528759608315 39.0 35.0 41.0 31.0 41.0 5 36.14221405873315 39.0 35.0 40.0 30.0 41.0 6 35.81853198863733 38.0 35.0 40.0 30.0 41.0 7 35.40739012874593 37.0 35.0 40.0 30.0 41.0 8 34.95141312763946 37.0 35.0 40.0 29.0 41.0 9 34.69716250906791 35.0 35.0 40.0 29.0 41.0 10-11 34.59673653126076 35.0 34.5 40.0 28.0 41.0 12-13 34.366561963405964 35.0 34.0 40.0 25.5 41.0 14-15 34.08250755353423 35.0 34.0 40.0 24.5 41.0 16-17 33.688343742596075 35.0 33.0 40.0 22.5 41.0 18-19 33.25957534116084 35.0 33.0 40.0 20.0 41.0 20-21 32.62911963772346 35.0 33.0 40.0 12.5 41.0 22-23 31.82976642054073 35.0 33.0 40.0 2.0 41.0 24-25 31.263857102031942 35.0 31.5 40.0 2.0 41.0 26-27 30.293053923807616 34.5 29.0 39.0 2.0 40.5 28-29 30.356559789551333 35.0 30.5 38.5 2.0 40.0 30-31 30.67277381408902 35.0 31.0 39.0 2.0 41.0 32-33 30.523461753592358 35.0 31.0 39.0 2.0 41.0 34-35 30.28411547320419 35.0 31.0 39.0 2.0 41.0 36-37 30.025095808368608 35.0 30.0 38.5 2.0 41.0 38-39 29.740037517311453 35.0 30.0 38.0 2.0 40.5 40-41 29.388891602814773 35.0 29.0 37.5 2.0 40.0 42-43 29.070507387441957 35.0 29.0 37.0 2.0 40.0 44-45 28.747859730197774 35.0 28.5 36.0 2.0 40.0 46-47 28.41573015869139 35.0 28.0 36.0 2.0 39.0 48-49 28.00224468810071 35.0 26.5 35.0 2.0 39.0 50-51 27.619210280134144 34.5 26.0 35.0 2.0 38.5 52-53 27.210723453937483 34.0 25.5 35.0 2.0 37.0 54-55 26.798090427467756 34.0 24.0 35.0 2.0 37.0 56-57 26.369401408364702 34.0 22.5 35.0 2.0 36.0 58-59 25.955929371707157 33.0 20.0 35.0 2.0 36.0 60-61 25.595148884617167 33.0 17.5 35.0 2.0 35.5 62-63 25.194995982032726 33.0 9.0 35.0 2.0 35.0 64-65 24.779038667744636 33.0 2.0 35.0 2.0 35.0 66-67 24.334629504336718 33.0 2.0 35.0 2.0 35.0 68-69 23.901856569559683 33.0 2.0 35.0 2.0 35.0 70-71 23.450483743719637 32.5 2.0 35.0 2.0 35.0 72-73 22.946984814770488 32.0 2.0 35.0 2.0 35.0 74-75 22.391701737373936 31.5 2.0 35.0 2.0 35.0 76-77 21.362050604404864 30.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.753823395266906 1101 2 0.4275437598444185 1101 3 0.8760111255070768 1101 4 0.7460268052889987 1101 5 0.32225376525557436 1101 6 0.26498653256160765 1101 7 0.3726767436808913 1101 8 0.7029948562560264 1101 9 0.36649581517094276 1101 10-11 0.7673945963059694 1101 12-13 0.6818962816857308 1101 14-15 0.7676944808891122 1101 16-17 1.0494772019935326 1101 18-19 1.1332742616581655 1101 20-21 1.2103831631008504 1101 22-23 1.0393773877723476 1101 24-25 1.3519354480097796 1101 26-27 0.9462002622667995 1101 28-29 1.0609159420385268 1101 30-31 1.3195202045860803 1101 32-33 1.3983107267581474 1101 34-35 1.4704016237576951 1101 36-37 1.6058485802303721 1101 38-39 1.0987504872219453 1101 40-41 1.081626751561732 1101 42-43 0.6412840740555517 1101 44-45 0.5155836917365626 1101 46-47 0.8014841630419482 1101 48-49 0.5710220108156641 1101 50-51 0.6201008467815221 1101 52-53 0.5499464583499787 1101 54-55 0.6020552873534406 1101 56-57 0.782583724500352 1101 58-59 0.740877878907952 1101 60-61 0.40427003483330637 1101 62-63 0.5678652569602143 1101 64-65 0.42463041246639577 1101 66-67 0.6463314861035698 1101 68-69 0.9880175976896588 1101 70-71 1.357734441161135 1101 72-73 1.0503009877965717 1101 74-75 0.8381309743443737 1101 76-77 0.580971119171334 1102 1 0.358697344846739 1102 2 0.6360031435979181 1102 3 0.6793724700448962 1102 4 0.7630576176139243 1102 5 0.6814134291211218 1102 6 0.577031350488781 1102 7 0.07351707981534616 1102 8 0.10943743328684263 1102 9 0.1296330700729058 1102 10-11 0.2505038400034536 1102 12-13 0.47828283630757795 1102 14-15 0.32242837444653105 1102 16-17 0.016956193590175417 1102 18-19 0.4541706202015803 1102 20-21 0.5563215384509874 1102 22-23 0.37601604323453586 1102 24-25 -0.1660477452675302 1102 26-27 -0.18119469571639257 1102 28-29 -0.21905604675699308 1102 30-31 -0.04123609793492733 1102 32-33 -0.15661924523065096 1102 34-35 -0.015676807614855903 1102 36-37 -0.005832092038538406 1102 38-39 -0.08878452678365889 1102 40-41 0.25126260590347016 1102 42-43 0.3626846342796419 1102 44-45 0.25390301946765703 1102 46-47 0.24190433110917553 1102 48-49 0.2727867166980147 1102 50-51 0.41696359187956133 1102 52-53 0.22112292893821106 1102 54-55 0.09351186998649297 1102 56-57 0.10350809424825158 1102 58-59 0.1568722766670554 1102 60-61 0.16412997881089808 1102 62-63 0.22960195163808805 1102 64-65 0.34992453011345503 1102 66-67 0.30156958134166345 1102 68-69 0.09098678536472704 1102 70-71 0.6437848613292019 1102 72-73 0.6777799793932111 1102 74-75 1.0963102460530578 1102 76-77 1.3237722396195117 1103 1 0.18623768329617008 1103 2 0.06439354107922668 1103 3 0.23214696827393766 1103 4 0.27268374905823833 1103 5 0.2887097691447309 1103 6 0.21252918598661807 1103 7 0.4487236913855952 1103 8 0.48948465878861214 1103 9 0.5677597918359893 1103 10-11 0.23530903480864396 1103 12-13 0.34131707479636475 1103 14-15 0.19392778412775868 1103 16-17 0.3020526123229601 1103 18-19 0.4863311869075986 1103 20-21 0.13562102684138466 1103 22-23 0.10040014398227015 1103 24-25 -0.08479626947059771 1103 26-27 0.21028110121395116 1103 28-29 0.008354421562568604 1103 30-31 -0.12731638128793676 1103 32-33 0.112341793730387 1103 34-35 0.2703877339042329 1103 36-37 0.04722182374265316 1103 38-39 0.27085734649458004 1103 40-41 0.15906268461263906 1103 42-43 0.41875704671569025 1103 44-45 0.44357637641374126 1103 46-47 0.6047575385078403 1103 48-49 0.9648803806098627 1103 50-51 1.0467392707461514 1103 52-53 0.8553496113467141 1103 54-55 0.6748381194945594 1103 56-57 0.7257394992069308 1103 58-59 0.70542402794608 1103 60-61 0.6979709862882686 1103 62-63 0.5835492164944398 1103 64-65 0.5690154392043603 1103 66-67 0.6359025211291502 1103 68-69 0.5703728538417074 1103 70-71 0.5802035941115697 1103 72-73 0.44140453665413304 1103 74-75 0.36147750303849335 1103 76-77 0.48066322741959056 1104 1 0.08695989310928809 1104 2 0.06866723626200866 1104 3 0.1573647480371747 1104 4 0.10336649792280639 1104 5 0.12527443298212404 1104 6 0.06158733504476288 1104 7 0.13567924197750614 1104 8 0.2955378193872278 1104 9 0.33125469169452515 1104 10-11 0.3910829905825963 1104 12-13 0.4579353459600881 1104 14-15 0.7976021196202723 1104 16-17 0.8037129503469274 1104 18-19 0.8274524734834401 1104 20-21 0.7827694148988584 1104 22-23 1.1362090934275884 1104 24-25 1.2377746485548649 1104 26-27 1.378496423974731 1104 28-29 1.341754764053821 1104 30-31 1.3277986510998225 1104 32-33 1.4061992875878815 1104 34-35 1.4569486750106257 1104 36-37 1.2201910739846298 1104 38-39 1.4382425002433727 1104 40-41 1.4836178182586828 1104 42-43 1.1892097308047376 1104 44-45 1.2235555291201656 1104 46-47 0.6932170724219162 1104 48-49 0.7463002302115278 1104 50-51 1.0348786497946207 1104 52-53 0.8822426200579052 1104 54-55 0.9410022174768429 1104 56-57 0.974589175329335 1104 58-59 1.0477216975164758 1104 60-61 1.0898442645251833 1104 62-63 0.9613394033755362 1104 64-65 0.7923569625458846 1104 66-67 0.8595232492953322 1104 68-69 0.7092493336272732 1104 70-71 0.2810821586265 1104 72-73 0.6188224504356832 1104 74-75 0.3897851495279596 1104 76-77 0.46369501954229264 1105 1 -0.11181567804197101 1105 2 -0.0771144570334954 1105 3 0.273058342183802 1105 4 0.2192691408972749 1105 5 -0.14638451878732894 1105 6 -0.3258100070487089 1105 7 -0.16460446714598476 1105 8 0.1788139131605604 1105 9 0.1792463297414102 1105 10-11 0.23505790472325572 1105 12-13 0.017461999685643548 1105 14-15 0.029026638061367294 1105 16-17 -0.10387253569160038 1105 18-19 -0.15641343662557006 1105 20-21 0.06342493229471557 1105 22-23 -0.2710478873100577 1105 24-25 -0.18359312806152772 1105 26-27 -0.19431229634780323 1105 28-29 0.010778207386653804 1105 30-31 0.027619466390248704 1105 32-33 0.005109247271320783 1105 34-35 0.03162390272452953 1105 36-37 0.2546020042519146 1105 38-39 0.17972376050915884 1105 40-41 0.3056351394472756 1105 42-43 0.08033262165138666 1105 44-45 0.2024191994203015 1105 46-47 0.11453258683135203 1105 48-49 0.009029810620667433 1105 50-51 0.16607172131918446 1105 52-53 0.4218648389618913 1105 54-55 0.5381053980054347 1105 56-57 0.24406058043610201 1105 58-59 -0.147634432093799 1105 60-61 -0.1767303211101776 1105 62-63 -0.24154248403673861 1105 64-65 0.12263171243389692 1105 66-67 -0.04493397035525604 1105 68-69 -0.4130069005074155 1105 70-71 -0.5203587850796403 1105 72-73 -0.6912492234481427 1105 74-75 -0.7673598407662041 1105 76-77 -0.6755016356764649 1106 1 -0.23674789956402265 1106 2 -0.102284086413448 1106 3 -0.6546934640210438 1106 4 -0.45488857412536987 1106 5 0.01565367163722442 1106 6 -0.15801980525203163 1106 7 -0.18696037376555807 1106 8 -0.14054362014248767 1106 9 -0.1723297946485829 1106 10-11 -0.575638638207991 1106 12-13 -0.14728366615547372 1106 14-15 -0.23652577485244564 1106 16-17 0.34509942966443674 1106 18-19 0.247418062414539 1106 20-21 0.3130475710390854 1106 22-23 0.7502638643789084 1106 24-25 0.5072527473433084 1106 26-27 0.43834300795924364 1106 28-29 0.25428612830932096 1106 30-31 0.15991206424771676 1106 32-33 0.38543077371592105 1106 34-35 0.11270894456400171 1106 36-37 0.2846150470292912 1106 38-39 0.5941067199006937 1106 40-41 0.6223804535730402 1106 42-43 0.43900899956952344 1106 44-45 0.20401290957754625 1106 46-47 0.42162771117738274 1106 48-49 0.12071775118077355 1106 50-51 -0.03489696136030318 1106 52-53 -0.052075252190999066 1106 54-55 0.0978127415287453 1106 56-57 0.31631976533578765 1106 58-59 0.07889955972882134 1106 60-61 0.03613073667372646 1106 62-63 0.25746098915229254 1106 64-65 0.38804124098878745 1106 66-67 0.46078140400176437 1106 68-69 0.07241156747916122 1106 70-71 0.4068617844061251 1106 72-73 0.18487736476646077 1106 74-75 0.3746012653785584 1106 76-77 0.18608372124133865 1107 1 0.15712123833360891 1107 2 0.16182538181310235 1107 3 0.1602648773856643 1107 4 -0.07888552740227084 1107 5 0.22105358745145054 1107 6 0.06109036452260597 1107 7 0.14858005210753333 1107 8 0.350279462793857 1107 9 0.5194567476547647 1107 10-11 0.5957395362970814 1107 12-13 0.7997761792366944 1107 14-15 0.5644650782968412 1107 16-17 0.6172332716758149 1107 18-19 0.8613962409284639 1107 20-21 0.8386173283034495 1107 22-23 0.5393985549293916 1107 24-25 0.33709727092534436 1107 26-27 0.13656708909829263 1107 28-29 0.13346284493066563 1107 30-31 0.29976066349004427 1107 32-33 0.42926416606837847 1107 34-35 0.643700666296624 1107 36-37 0.5977627262414202 1107 38-39 0.7146159769946792 1107 40-41 0.7295569563892563 1107 42-43 0.9812956454347344 1107 44-45 0.9868069417418575 1107 46-47 0.6732429421203321 1107 48-49 0.8820310491917169 1107 50-51 0.41136445549956946 1107 52-53 0.4745702121336066 1107 54-55 0.6456349358375206 1107 56-57 0.510384668555556 1107 58-59 0.4130903407188704 1107 60-61 0.25923973052014304 1107 62-63 0.18110249140058698 1107 64-65 0.04279606663738278 1107 66-67 0.26873762740139995 1107 68-69 0.2452796964838342 1107 70-71 0.2810140804727759 1107 72-73 0.5225190941827051 1107 74-75 0.3469435673916692 1107 76-77 0.4644847261254519 1108 1 -0.2301961118165039 1108 2 -0.3380085042266785 1108 3 -0.25882554297326266 1108 4 -0.3912280966717887 1108 5 -0.14480787954623509 1108 6 -0.10292753954205125 1108 7 -0.06125510243884946 1108 8 0.055412082139184804 1108 9 0.4806162835021581 1108 10-11 0.2027169266769704 1108 12-13 0.1787727301068287 1108 14-15 0.10277095752292809 1108 16-17 -0.013235652932607422 1108 18-19 0.07835013373361477 1108 20-21 0.34974394338418335 1108 22-23 0.4278968243239518 1108 24-25 0.31395910639399816 1108 26-27 -0.004367015003818864 1108 28-29 0.09257464868665366 1108 30-31 0.46935999661799954 1108 32-33 0.32740610591700303 1108 34-35 0.5913838295896667 1108 36-37 0.7094094290303232 1108 38-39 0.478241852113225 1108 40-41 0.19096113279624305 1108 42-43 0.2461447233512395 1108 44-45 0.264057181852035 1108 46-47 0.27961930198961227 1108 48-49 0.16144379103854334 1108 50-51 -0.08805867602040252 1108 52-53 -0.2411483968582928 1108 54-55 0.055776344121468924 1108 56-57 0.12228507266551958 1108 58-59 0.04975682617030941 1108 60-61 0.047278317282977156 1108 62-63 -0.25124080272984983 1108 64-65 -0.37125052983858353 1108 66-67 -0.25980075096391886 1108 68-69 -0.03608412933208527 1108 70-71 -0.18647892763962304 1108 72-73 0.07237744427844817 1108 74-75 0.10491250710136057 1108 76-77 0.15982568257943086 1109 1 0.5828203104886924 1109 2 0.5940863262380631 1109 3 0.08298910657292424 1109 4 0.34801240965732205 1109 5 0.2941078233344996 1109 6 0.3951145331289254 1109 7 0.16971961146091274 1109 8 0.47381356348575565 1109 9 0.7007723105792323 1109 10-11 0.26545863204685105 1109 12-13 0.11229730285369754 1109 14-15 0.07928190066714791 1109 16-17 0.05581695308384127 1109 18-19 -0.29960876497020905 1109 20-21 0.08579712615442503 1109 22-23 0.6000305097806518 1109 24-25 0.3037171733581481 1109 26-27 0.4376479805946403 1109 28-29 0.5005642064075637 1109 30-31 0.5770459997142581 1109 32-33 0.4330552574818256 1109 34-35 0.6041604437413852 1109 36-37 0.6667357198963657 1109 38-39 0.3624812960011532 1109 40-41 0.4428720091584424 1109 42-43 0.5119432237913948 1109 44-45 0.4108288784188332 1109 46-47 0.32694953906577595 1109 48-49 0.3359512736600436 1109 50-51 0.3091516569790187 1109 52-53 0.22240683490566582 1109 54-55 0.33537443779842846 1109 56-57 0.47198910690648077 1109 58-59 0.18980175225475548 1109 60-61 0.35503413794290495 1109 62-63 0.24016253029992995 1109 64-65 0.29536756808813536 1109 66-67 0.4290921853199663 1109 68-69 0.6665021560699707 1109 70-71 0.7293544815823658 1109 72-73 0.6823188582358881 1109 74-75 0.7208491248957358 1109 76-77 0.7882930352795476 1110 1 -0.024192638732898786 1110 2 0.22821989236638984 1110 3 -0.1524830307761249 1110 4 0.2961282251672941 1110 5 -0.03934190749628641 1110 6 -0.1638553490186112 1110 7 -0.28740246041454043 1110 8 -0.1350617456950971 1110 9 -0.6501370448696235 1110 10-11 -0.27608072485697477 1110 12-13 -0.13349056270720183 1110 14-15 -0.09691481110355227 1110 16-17 -0.143848404110976 1110 18-19 -0.14011509408413048 1110 20-21 -0.07651918404490488 1110 22-23 0.10711620743815331 1110 24-25 0.30840217263066805 1110 26-27 0.17883814500610384 1110 28-29 0.3309439532430076 1110 30-31 0.40084928794356145 1110 32-33 0.1661557958202522 1110 34-35 0.4385268048646189 1110 36-37 0.5066309621486553 1110 38-39 0.2865028295381826 1110 40-41 0.27454667815306166 1110 42-43 0.25598512689047936 1110 44-45 0.2804054825218465 1110 46-47 0.24894866608454436 1110 48-49 -0.0585925936468108 1110 50-51 -0.011377951634393924 1110 52-53 0.26649238706628964 1110 54-55 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2313 76-77 1.0818733205426803 2314 1 -0.31964311708653526 2314 2 -0.5041223197610805 2314 3 -0.3063133012696184 2314 4 -0.31937180924663977 2314 5 -0.55446338867187 2314 6 -0.6332338334291023 2314 7 -0.41869848904693185 2314 8 -0.5087832681673916 2314 9 -0.2274926784300888 2314 10-11 -0.6099096187932389 2314 12-13 -0.18562078502119306 2314 14-15 -0.36834436578728713 2314 16-17 -0.4646206527172154 2314 18-19 0.09301863845079339 2314 20-21 -0.24735681911958096 2314 22-23 -0.312658037176071 2314 24-25 0.037949973580492014 2314 26-27 -0.06543193552248994 2314 28-29 -0.21536343198653185 2314 30-31 -0.400719987299059 2314 32-33 -0.4706739928782113 2314 34-35 -0.5306383131751637 2314 36-37 -0.480491344961262 2314 38-39 -0.0930759439493265 2314 40-41 0.19564241770843083 2314 42-43 0.05913744294801759 2314 44-45 0.009939517899365313 2314 46-47 0.050221981522632575 2314 48-49 0.20933362288564084 2314 50-51 0.18671551660154506 2314 52-53 0.19799489928323766 2314 54-55 -0.06045163158179889 2314 56-57 0.17035440163348525 2314 58-59 0.48065043463682855 2314 60-61 0.19912570165093157 2314 62-63 0.2388006970044927 2314 64-65 0.4533377037661488 2314 66-67 0.48993000335478243 2314 68-69 0.29136887832167346 2314 70-71 0.22829583937311426 2314 72-73 0.14170641224179903 2314 74-75 0.2530909703188087 2314 76-77 0.1720243069134959 2315 1 -1.1566344886740723 2315 2 -0.914435551565191 2315 3 -0.9458806233184376 2315 4 -0.8361099864355666 2315 5 -0.9197564246471615 2315 6 -0.9775805730207807 2315 7 -1.0107348886885887 2315 8 -1.2229022742497193 2315 9 -1.3100519692971773 2315 10-11 -1.5802779372743885 2315 12-13 -1.3404685697666565 2315 14-15 -1.6098888359021757 2315 16-17 -1.6870989245200647 2315 18-19 -1.2603906846353183 2315 20-21 -1.216597471672749 2315 22-23 -1.3113967801625392 2315 24-25 -1.4128531445926136 2315 26-27 -1.4873420522968175 2315 28-29 -1.2814576215601399 2315 30-31 -1.1678333982723856 2315 32-33 -1.5212480416311003 2315 34-35 -1.1425778199883077 2315 36-37 -1.5765688749406763 2315 38-39 -1.320477440169487 2315 40-41 -1.1708352568636862 2315 42-43 -1.0952231272501685 2315 44-45 -0.8293759456616989 2315 46-47 -0.9219989309830652 2315 48-49 -0.8292211573177326 2315 50-51 -0.8386157107076144 2315 52-53 -0.8993438877097049 2315 54-55 -0.8505544967176704 2315 56-57 -1.0068856065391465 2315 58-59 -0.7059173746265337 2315 60-61 -1.2261006173645796 2315 62-63 -1.2509717266521285 2315 64-65 -1.1343668084692204 2315 66-67 -1.3629017347438257 2315 68-69 -1.4067859930379747 2315 70-71 -1.967986792214102 2315 72-73 -2.935572101596666 2315 74-75 -3.4078686065903625 2315 76-77 -3.239624835746067 2316 1 -2.39367073752134 2316 2 -2.205206637611866 2316 3 -1.7759685852961624 2316 4 -2.184020889464584 2316 5 -2.5292300715223845 2316 6 -2.320845097387668 2316 7 -2.1413477850495184 2316 8 -1.8067144328650215 2316 9 -2.2498263893865555 2316 10-11 -2.1625206130210017 2316 12-13 -2.4850826720579278 2316 14-15 -2.3213167992986428 2316 16-17 -3.7778185811846043 2316 18-19 -3.5799799589489965 2316 20-21 -3.0199332878038376 2316 22-23 -2.724826942608452 2316 24-25 -2.4340721982919824 2316 26-27 -2.3550621345838287 2316 28-29 -2.3487857764867215 2316 30-31 -2.3616157633145924 2316 32-33 -2.482764676376082 2316 34-35 -2.613546820484867 2316 36-37 -2.868998115204562 2316 38-39 -2.7072529952521265 2316 40-41 -2.593859659218797 2316 42-43 -2.6729524313574267 2316 44-45 -2.516534560969923 2316 46-47 -2.4979991437942957 2316 48-49 -2.2705916616803634 2316 50-51 -2.7473285574125867 2316 52-53 -3.5677573799420976 2316 54-55 -2.637601386126761 2316 56-57 -2.5877982120580505 2316 58-59 -2.7452963205015415 2316 60-61 -2.3347052850243664 2316 62-63 -2.303075011038878 2316 64-65 -1.9707061005171767 2316 66-67 -1.8731344083623256 2316 68-69 -2.147732648354708 2316 70-71 -1.9171160395716988 2316 72-73 -1.99288926127603 2316 74-75 -1.9870166522738408 2316 76-77 -2.194863539016268 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9283.0 3 1574.0 4 2855.0 5 3591.0 6 3608.0 7 3344.0 8 4368.0 9 4933.0 10 7635.0 11 8778.0 12 5582.0 13 3270.0 14 2829.0 15 2815.0 16 2895.0 17 3044.0 18 3158.0 19 3301.0 20 3529.0 21 3572.0 22 3934.0 23 4398.0 24 5173.0 25 5870.0 26 6777.0 27 7608.0 28 8602.0 29 9886.0 30 11614.0 31 13648.0 32 17118.0 33 22727.0 34 45571.0 35 49205.0 36 32888.0 37 37685.0 38 32827.0 39 9808.0 40 108.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 15.514630082909504 29.589947283822145 40.76831752825128 14.127105105017076 2 16.318468972458508 29.64411524724805 40.69735627073429 13.340059509559152 3 14.948063242651658 30.46239837398374 40.44324577861163 14.146292604752972 4 15.097206682073807 30.702067260476774 40.771350535259714 13.429375522189705 5 14.851385981155444 30.52920612973282 40.70682962459905 13.912578264512677 6 14.37498320536842 31.95694711462122 40.85920113739352 12.808868542616835 7 15.486468395311453 31.345000952757868 39.632284322491444 13.536246329439242 8 14.12663744790216 31.923210350674037 39.97034148820256 13.979810713221246 9 14.25419898613571 32.26726928479815 39.81041959323276 13.668112135833384 10-11 14.026138377778835 32.425855959191004 39.821387344666725 13.726618318363437 12-13 14.631977416726622 32.4975356039572 39.08843608877445 13.782050890541727 14-15 15.104255724538202 32.6159441885006 38.65400681033177 13.625793276629421 16-17 14.207408004514127 33.45791150796995 38.61544793792587 13.719232549590052 18-19 14.185292899780505 33.67293967335741 38.06774944942732 14.074017977434766 20-21 14.510827143166019 33.84796924644256 37.29198655814482 14.3492170522466 22-23 14.358516448280536 34.4164894455187 36.331088599987545 14.893905506213226 24-25 14.665946801649502 34.857183340825145 35.26968065354623 15.207189203979127 26-27 14.916080328349457 35.49056972539822 34.38373399785009 15.209615948402227 28-29 14.401189157366707 35.93842177711605 33.88842000608258 15.771969059434662 30-31 14.264880828787721 35.92553417576807 33.74156323512528 16.068021760318928 32-33 14.902583006899988 35.84024370432529 33.01404548935687 16.243127799417856 34-35 14.767730461803044 35.19196193007485 33.85094002227911 16.18936758584299 36-37 15.153388021125968 35.501057764184544 33.79849714428099 15.547057070408496 38-39 15.586160564326503 35.39231074602254 33.89635691819098 15.12517177145998 40-41 15.224388140609484 35.637534460414344 34.22723780464717 14.910839594329008 42-43 15.384145589217857 35.58624969005248 33.938977899978866 15.090626820750789 44-45 15.607830970638744 35.61484210294883 33.747853298774395 15.02947362763803 46-47 14.979966284723071 35.88881288021304 33.68730302215924 15.443917812904646 48-49 15.018170491122419 35.66510105236155 33.37836764858576 15.938360807930273 50-51 14.67752162052212 35.8228137281611 33.68794369562801 15.811720955688772 52-53 14.209446739160978 35.642791434089915 34.631853760382725 15.515908066366379 54-55 14.03931619337547 35.556576275907226 34.72539500656003 15.678712524157273 56-57 13.651754979588146 36.1289794562214 34.294233621045585 15.925031943144866 58-59 13.57147481350918 35.94451020126473 33.605481065089485 16.87853392013661 60-61 13.792020912733314 35.456122349262195 33.60842372715724 17.143433010847257 62-63 13.981829753808677 35.08992891443957 33.84351892459116 17.084722407160598 64-65 14.14222608797631 34.574826774039074 34.38193270330229 16.90101443468233 66-67 13.964499932811297 34.86262964365555 34.161301750571106 17.011568672962042 68-69 13.4346903394409 35.07238302587805 34.26150254186658 17.231424092814475 70-71 13.39268259305599 34.85249843603378 34.32490420706913 17.4299147638411 72-73 13.855600600839171 34.272765827944404 34.08136750799632 17.790266063220113 74-75 14.096288501283935 33.81231918864514 34.05223989136958 18.03915241870135 76-77 13.961837034284578 33.74223680302351 34.820680997845066 17.47524516484685 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 83585.0 1 46523.5 2 7833.5 3 5628.0 4 5051.0 5 4693.0 6 3883.5 7 3349.0 8 3037.5 9 2809.0 10 2639.5 11 2375.0 12 2263.0 13 2246.0 14 2249.0 15 2299.0 16 2408.0 17 2470.0 18 2541.5 19 2764.0 20 3008.5 21 3406.5 22 3711.0 23 3853.5 24 4161.5 25 4729.0 26 5131.0 27 5449.5 28 6078.0 29 6661.0 30 6934.0 31 7360.5 32 8175.0 33 8936.5 34 9294.5 35 9279.0 36 9616.5 37 10019.0 38 9851.5 39 9619.0 40 9533.0 41 9374.5 42 9305.5 43 9309.0 44 9367.5 45 9604.0 46 10145.5 47 12158.5 48 13808.0 49 13076.0 50 13888.5 51 12308.5 52 9184.0 53 8707.5 54 7718.5 55 6866.0 56 6322.5 57 6119.0 58 6534.0 59 6256.0 60 5334.0 61 5105.0 62 4621.0 63 3211.0 64 1891.5 65 1498.0 66 1315.5 67 1027.0 68 841.5 69 646.0 70 530.0 71 462.5 72 383.0 73 357.0 74 343.0 75 338.0 76 264.5 77 155.0 78 114.0 79 101.0 80 98.5 81 97.5 82 58.0 83 30.0 84 27.0 85 22.0 86 18.0 87 16.0 88 14.5 89 14.5 90 13.5 91 11.0 92 7.5 93 5.0 94 5.0 95 5.5 96 7.0 97 6.5 98 5.0 99 10.0 100 16.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011968413159392396 2 0.015876466435928688 3 0.016364973095495724 4 0.018807506393330908 5 0.015143706446578132 6 0.013189679808309988 7 0.017830493074196836 8 0.020761533031599055 9 0.02100578636138257 10-11 0.021860673015624884 12-13 0.01990664637735674 14-15 0.008060359882856102 16-17 0.00720547322861379 18-19 0.015754339771036928 20-21 0.02259343300497544 22-23 0.02015089970714026 24-25 0.01831899973376387 26-27 0.022227053010300164 28-29 0.011113526505150082 30-31 0.010258639850907768 32-33 0.015265833111469894 34-35 0.01441094645722758 36-37 0.013922439797660542 38-39 0.017952619739088592 40-41 0.015754339771036928 42-43 0.016487099760387484 44-45 0.015143706446578132 46-47 0.024669586308135347 48-49 0.02283768633475896 50-51 0.03309632618566673 52-53 0.03358483284523376 54-55 0.027844879595321084 56-57 0.021982799680516644 58-59 0.01819687306887211 60-61 0.022227053010300164 62-63 0.02821125958999636 64-65 0.029066146244238675 66-67 0.02833338625488812 68-69 0.04017967274938876 70-71 0.04762939930778606 72-73 0.07730617887648354 74-75 0.07755043220626705 76-77 0.08536653875933964 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 409411.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.04151944197299 #Duplication Level Percentage of deduplicated Percentage of total 1 82.47008552020817 38.79518131380048 2 9.350863756352428 8.797576783873865 3 3.410535958085926 4.81310380739541 4 1.610860802002022 3.0310935894276128 5 0.8610832554521726 2.025333235125538 6 0.5391936738660894 1.5218693815296291 7 0.34387596958646377 1.132351367625026 8 0.2480549221690422 0.9335104355113674 9 0.18359015855275584 0.7772724011622929 >10 0.8815909823784114 7.339467037290882 >50 0.05091176234177457 1.6654793647392974 >100 0.043119145656809 3.6950914152396397 >500 0.00415606223198159 1.371973756954718 >1k 0.0010390155579953974 0.7292429089335373 >5k 0.0 0.0 >10k+ 0.0010390155579953974 23.371453201390718 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 53303 13.019435237450875 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 42331 10.339487703066112 No Hit CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA 1539 0.3759058745368346 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 1445 0.3529460615371839 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 978 0.23887975652828086 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 918 0.2242245567412698 No Hit AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG 735 0.17952619739088593 No Hit AGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGC 725 0.17708366409305074 No Hit TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 618 0.1509485578062143 No Hit CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG 558 0.1362933580192032 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 556 0.13580485135963616 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 526 0.12847725146613062 No Hit TACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT 486 0.11870711827478989 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 466 0.11382205167911952 No Hit TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 420 0.10258639850907766 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 4.885066595670365E-4 0.0 2 0.0 0.0 0.0 0.0029310399574022194 0.0 3 0.0 0.0 0.0 0.004885066595670366 0.0 4 0.0 0.0 0.0 0.00708334656372203 0.0 5 0.0 0.0 0.0 0.01147990649982536 0.0 6 0.0 0.0 0.0 0.018807506393330908 0.0 7 0.0 0.0 0.0 0.029798906233589228 0.0 8 0.0 0.0 0.0 0.04396559936103329 0.0 9 0.0 0.0 0.0 0.0534914792225905 0.0 10 0.0 0.0 0.0 0.06497138572241586 0.0 11 0.0 0.0 0.0 0.08109210548812806 0.0 12 0.0 0.0 0.0 0.09989961188145897 0.0 13 0.0 0.0 0.0 0.12114965157262507 0.0 14 0.0 0.0 0.0 0.147040504529678 0.0 15 0.0 0.0 0.0 0.1763509041037002 0.0 16 0.0 0.0 0.0 0.21640845018819718 0.0 17 0.0 0.0 0.0 0.24816138306005456 0.0 18 0.0 0.0 0.0 0.2789373026127779 0.0 19 0.0 0.0 0.0 0.3045839022400473 0.0 20 0.0 0.0 0.0 0.33609258178212115 0.0 21 0.0 0.0 0.0 0.36100642142004 0.0 22 0.0 0.0 0.0 0.38933980767492815 0.0 23 0.0 0.0 0.0 0.4293973537594251 0.0 24 0.0 0.0 0.0 0.46921064651413863 0.0 25 0.0 0.0 0.0 0.508535432609285 0.0 26 0.0 0.0 0.0 0.5485929786937821 0.0 27 0.0 0.0 0.0 0.5945126046930834 0.0 28 0.0 0.0 0.0 0.634325897447797 0.0 29 0.0 0.0 0.0 0.681955296755583 0.0 30 0.0 0.0 0.0 0.7149294962763579 0.0 31 0.0 0.0 0.0 0.7571853223289067 0.0 32 0.0 0.0 0.0 0.7969986150836201 0.0 33 0.0 0.0 0.0 0.8463377876998908 0.0 34 0.0 0.0 0.0 0.9025160535501 0.0 35 0.0 0.0 0.0 0.9601598393790103 0.0 36 0.0 0.0 0.0 1.0192691451866218 0.0 37 0.0 0.0 0.0 1.0869273175366563 0.0 38 0.0 0.0 0.0 1.1406630500890302 0.0 39 0.0 0.0 0.0 1.1995281025668583 0.0 40 0.0 0.0 0.0 1.2561948750766345 0.0 41 0.0 0.0 0.0 1.3211662607990504 0.0 42 0.0 0.0 0.0 1.384672126542765 0.0 43 0.0 0.0 0.0 1.4586808854671711 0.0 44 0.0 0.0 0.0 1.5431925375722684 0.0 45 0.0 0.0 0.0 1.6257501630390976 0.0 46 0.0 0.0 0.0 1.7339543881331962 0.0 47 0.0 0.0 0.0 1.8316557200466035 0.0 48 0.0 0.0 0.0 1.946454785044857 0.0 49 0.0 0.0 0.0 2.051972223511337 0.0 50 0.0 0.0 0.0 2.1562683953288992 0.0 51 0.0 0.0 0.0 2.2681364203697507 0.0 52 0.0 0.0 0.0 2.362662458995972 0.0 53 0.0 0.0 0.0 2.466714377483751 0.0 54 0.0 0.0 0.0 2.5861542557478914 0.0 55 0.0 0.0 0.0 2.7129217339055374 0.0 56 0.0 0.0 0.0 2.855077171839545 0.0 57 0.0 0.0 0.0 2.99845387642247 0.0 58 0.0 0.0 0.0 3.1647903940050464 0.0 59 0.0 0.0 0.0 3.3269746049813023 0.0 60 0.0 0.0 0.0 3.4815869627342693 0.0 61 0.0 0.0 0.0 3.6498775069551135 0.0 62 0.0 0.0 0.0 3.828182437697082 0.0 63 0.0 0.0 0.0 3.99256492864139 0.0 64 0.0 0.0 0.0 4.169892846064224 0.0 65 0.0 0.0 0.0 4.356013883359265 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCGAT 20 0.006568126 53.25339 18 TCATCGG 35 0.0011699102 40.574013 21 ATCGGAG 35 0.0011699102 40.574013 23 CGGTTAC 60 1.1044351E-5 35.48925 4 GAGGTGC 50 0.006720853 28.401808 13 CGAGGTG 50 0.00672888 28.394869 12 GCGGTTA 75 5.075808E-5 28.3914 3 GTGGGTA 75 5.075808E-5 28.3914 1 GCCTAGT 80 7.857598E-5 26.626696 17 GGCGGTT 80 7.8772246E-5 26.616938 2 CACGGGG 75 0.0016853474 23.668173 40 CTGCCTA 105 1.8256447E-5 23.665281 15 CTAGTGG 95 2.5219622E-4 22.422482 19 GAGTACT 160 4.2229658E-8 22.180782 6 GGTCACT 80 0.0024579233 22.180782 34 GTTACAA 115 3.6938312E-5 21.60215 6 ATTTGCG 150 5.5694363E-7 21.293549 3 GGTTACA 120 5.1277988E-5 20.702063 5 CCTAGTG 105 4.9584545E-4 20.287006 18 TGCCTAG 115 9.146572E-4 18.522917 16 >>END_MODULE