FastQCFastQC Report
Thu 26 May 2016
SRR936154_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936154_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences411223
Sequences flagged as poor quality0
Sequence length77
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5394013.11697059746172No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4234810.298062122011657No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA14590.3547953300277465No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC14380.34968861177511956No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9170.22299336369804218No Hit
AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG8480.20621414658226803No Hit
AGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGC7270.17678972236475102No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG7060.1716830041121241No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6280.1527151934595098No Hit
CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG5680.13812456988057575No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT5480.13326102868759773No Hit
TACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT5350.130099726912162No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC5180.1259657168981307No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG4450.10821379154376093No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4370.10626837506656972No Hit
GACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTAC4190.10189118799288951No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAG400.002256843735.4944652
TTTAGTC500.00672010928.40248114
ATTTGCG1102.6179576E-522.5873893
AGGTCTC1003.5580387E-421.30963962
TAGCCGT2850.021.17213624
GTAGCCG2800.020.28255323
GAGGTGT1054.9658574E-420.2825554
GCGCAAG900.00485708719.72154638
GGTAGCC2950.019.25123822
GTGGTAG3001.8189894E-1218.93498820
AGCCGTT3001.8189894E-1218.93038225
CTCGGTG950.00661953718.69266570
TGAGGTG1159.1526884E-418.52110553
TGCGCGC1351.2648193E-418.4067766
TTGCGCG1351.2648193E-418.4067765
CGCGCCT1551.752591E-518.3219538
GATGTGG3203.6379788E-1217.7515517
TGTGGTA3203.6379788E-1217.7515519
TTGAGGC1000.00892262917.74939360
TTGAGGT1200.001217894717.74939252
Produced by FastQC (version 0.11.5)