##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936154_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 411223 Sequences flagged as poor quality 0 Sequence length 77 %GC 30 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.86407861427984 40.0 35.0 41.0 31.0 41.0 2 36.65013873251253 39.0 35.0 41.0 31.0 41.0 3 36.45305102097888 39.0 35.0 41.0 31.0 41.0 4 36.29596836752808 39.0 35.0 41.0 30.0 41.0 5 36.11686603132607 38.0 35.0 40.0 30.0 41.0 6 35.80755453853505 38.0 35.0 40.0 30.0 41.0 7 35.41646260058411 37.0 35.0 40.0 30.0 41.0 8 35.00435773290891 37.0 35.0 40.0 30.0 41.0 9 34.86252957640988 36.0 35.0 40.0 30.0 41.0 10-11 34.712948935249244 36.0 35.0 40.0 28.0 41.0 12-13 34.34163823521544 35.0 34.0 40.0 25.0 41.0 14-15 34.01816046281458 35.0 34.0 40.0 23.5 41.0 16-17 33.65768694844403 35.0 33.0 40.0 22.0 41.0 18-19 33.237952400522346 35.0 33.0 40.0 20.0 41.0 20-21 32.62320930492701 35.0 33.0 40.0 12.0 41.0 22-23 31.801539553964638 35.0 32.5 40.0 2.0 41.0 24-25 31.233367297062664 35.0 31.5 40.0 2.0 41.0 26-27 30.23265600416319 34.5 29.0 39.0 2.0 40.0 28-29 30.289482835347243 35.0 30.5 38.5 2.0 40.0 30-31 30.653743832421824 35.0 31.0 39.0 2.0 41.0 32-33 30.52816963058973 35.0 31.0 39.0 2.0 41.0 34-35 30.299382573445552 35.0 31.0 39.0 2.0 41.0 36-37 30.0205679157051 35.0 30.0 38.5 2.0 41.0 38-39 29.708371127101355 35.0 30.0 38.0 2.0 40.0 40-41 29.36994647672917 35.0 29.0 37.5 2.0 40.0 42-43 29.057928423264265 35.0 29.0 37.0 2.0 40.0 44-45 28.740573849225356 35.0 28.5 36.0 2.0 40.0 46-47 28.374847710366396 35.0 27.5 36.0 2.0 39.0 48-49 28.005827738234487 35.0 26.0 35.0 2.0 39.0 50-51 27.609822407793338 34.5 26.0 35.0 2.0 38.5 52-53 27.168218946897426 34.0 25.5 35.0 2.0 37.0 54-55 26.74659977676346 34.0 24.0 35.0 2.0 37.0 56-57 26.33957609374962 34.0 22.0 35.0 2.0 36.0 58-59 25.91007920276833 33.0 20.0 35.0 2.0 36.0 60-61 25.53752829973032 33.0 16.5 35.0 2.0 35.5 62-63 25.111675416987865 33.0 6.5 35.0 2.0 35.0 64-65 24.6827135155378 33.0 2.0 35.0 2.0 35.0 66-67 24.26474564895446 33.0 2.0 35.0 2.0 35.0 68-69 23.82705977048949 33.0 2.0 35.0 2.0 35.0 70-71 23.350196851829786 32.5 2.0 35.0 2.0 35.0 72-73 22.849325062070946 32.0 2.0 35.0 2.0 35.0 74-75 22.31041551664182 31.5 2.0 35.0 2.0 35.0 76-77 21.2713661444034 30.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1969193427863587 1101 2 0.5606633339076055 1101 3 0.47615714756324934 1101 4 0.3712443026193526 1101 5 0.316398729861433 1101 6 0.6611234660813352 1101 7 0.696869477023462 1101 8 0.7913938299719518 1101 9 0.630486332831687 1101 10-11 0.888362805885059 1101 12-13 0.9905313688881137 1101 14-15 1.132273775247718 1101 16-17 1.427779263914836 1101 18-19 1.4458859358840144 1101 20-21 1.2796471989507907 1101 22-23 1.2145872971628968 1101 24-25 1.6384346616626395 1101 26-27 1.7933411037226854 1101 28-29 1.5112829957123033 1101 30-31 1.3430966810477543 1101 32-33 1.2600852461769065 1101 34-35 1.4233437698961673 1101 36-37 1.7070503595371562 1101 38-39 1.4734662219313641 1101 40-41 1.7490184226504404 1101 42-43 1.5979827230138284 1101 44-45 1.4531055473818277 1101 46-47 1.519909023119972 1101 48-49 1.7022637978405903 1101 50-51 1.7421591956323645 1101 52-53 1.6900711683484033 1101 54-55 1.6738123002722425 1101 56-57 1.5518134132800903 1101 58-59 1.893613503248389 1101 60-61 1.8854710420377998 1101 62-63 1.6349697801129146 1101 64-65 1.3834239564132282 1101 66-67 1.4118242804316772 1101 68-69 1.2673760139133776 1101 70-71 1.1815588559630754 1101 72-73 1.3528310670739039 1101 74-75 1.4609943693525658 1101 76-77 1.3977428876325533 1102 1 0.814077704266083 1102 2 1.0259592312039487 1102 3 1.2523332932948747 1102 4 0.9083815342948469 1102 5 1.233888445089299 1102 6 1.1609713955205905 1102 7 1.1136148468750733 1102 8 1.2106923845012503 1102 9 1.2979320276340687 1102 10-11 1.4549096836363304 1102 12-13 1.4131834794674276 1102 14-15 1.4991199918922433 1102 16-17 1.4761617133713756 1102 18-19 1.3398367290520454 1102 20-21 1.4573376473188375 1102 22-23 1.6660767882867766 1102 24-25 2.1418042251400955 1102 26-27 1.8551657903772956 1102 28-29 1.9426751616773181 1102 30-31 2.200406472791123 1102 32-33 2.0600564328074995 1102 34-35 1.85056199915455 1102 36-37 1.9319347058738572 1102 38-39 1.7138297506086708 1102 40-41 2.251815720596685 1102 42-43 2.277930218651804 1102 44-45 2.2054266243615466 1102 46-47 2.107185519414255 1102 48-49 2.0190107684349456 1102 50-51 1.5288041989138854 1102 52-53 1.5425747300010109 1102 54-55 1.935525735306097 1102 56-57 1.5500285851198257 1102 58-59 1.7494225986686622 1102 60-61 1.6943543638991407 1102 62-63 1.9887499341043657 1102 64-65 1.7309291988457183 1102 66-67 2.0053756882849214 1102 68-69 2.017756190315229 1102 70-71 2.0424983318799015 1102 72-73 1.9559068942577191 1102 74-75 1.9768217132401311 1102 76-77 2.0415736250947347 1103 1 -0.01767202813468316 1103 2 -0.028887614057708788 1103 3 -0.0709778989513481 1103 4 0.07245285498869691 1103 5 -0.12228246650764873 1103 6 -2.1865125428632837E-4 1103 7 0.1550890620395151 1103 8 -0.11951773588174319 1103 9 0.011138966152230978 1103 10-11 0.048573080460329265 1103 12-13 -0.003956068033268423 1103 14-15 0.17167017591253142 1103 16-17 -0.04006286470161058 1103 18-19 -0.20777393972608849 1103 20-21 0.18811082581118654 1103 22-23 0.48398467549920454 1103 24-25 0.7925908935663166 1103 26-27 0.7845232589618796 1103 28-29 0.6466077706598519 1103 30-31 0.6046426052857328 1103 32-33 0.47048767080219633 1103 34-35 0.47094133022978113 1103 36-37 0.20275612554276634 1103 38-39 0.24456700482060967 1103 40-41 0.4009711334326518 1103 42-43 0.2143369108320421 1103 44-45 0.04762005828286675 1103 46-47 -0.3754425249852247 1103 48-49 -0.05826309188558554 1103 50-51 0.10489035853499473 1103 52-53 0.4892198420994269 1103 54-55 0.162493872668815 1103 56-57 0.25072067737924186 1103 58-59 0.21619997077089437 1103 60-61 0.19797245214090609 1103 62-63 -0.3226255992612259 1103 64-65 -0.08930276936495929 1103 66-67 -0.03401271916973414 1103 68-69 -0.12913745915177444 1103 70-71 -0.1570463828519877 1103 72-73 -0.17652539947092905 1103 74-75 0.1137555392493077 1103 76-77 -0.05334345580087785 1104 1 0.35289927779791697 1104 2 0.4977566760494554 1104 3 0.6789886769424811 1104 4 0.28309509039291925 1104 5 0.5761758026162198 1104 6 0.5472400611787762 1104 7 0.5954921215766475 1104 8 0.6813590311979709 1104 9 0.6747798716288571 1104 10-11 1.2061378352681231 1104 12-13 1.3104381112465902 1104 14-15 1.4590365674460344 1104 16-17 1.4296267484935257 1104 18-19 1.1539065539655482 1104 20-21 1.342565379581778 1104 22-23 1.6431537874031115 1104 24-25 1.6600168151920691 1104 26-27 1.4806207306921202 1104 28-29 1.5010123641875346 1104 30-31 1.6610906431147896 1104 32-33 1.6538452805915895 1104 34-35 1.4819255470150168 1104 36-37 1.3451815194194694 1104 38-39 1.622171446356571 1104 40-41 1.799073347867644 1104 42-43 1.918688377622555 1104 44-45 1.6712892511082948 1104 46-47 1.7093280449133736 1104 48-49 1.8096483588613523 1104 50-51 1.646047747373455 1104 52-53 1.5100624110410443 1104 54-55 1.3789483842194805 1104 56-57 1.4136438441549757 1104 58-59 1.2520024659683777 1104 60-61 1.4847935029948012 1104 62-63 1.7227502636084608 1104 64-65 1.6352157475898572 1104 66-67 1.5820041125064677 1104 68-69 1.3255429712099804 1104 70-71 1.2492359417770054 1104 72-73 1.1818830501431101 1104 74-75 1.1319769546018783 1104 76-77 0.8909790199754397 1105 1 -0.05632940222923821 1105 2 0.16860695499583045 1105 3 0.2529532154034797 1105 4 0.3312194647760549 1105 5 0.2402991690124523 1105 6 0.3188400303791781 1105 7 0.26130946595116455 1105 8 0.3428014324467554 1105 9 0.047994019844935565 1105 10-11 -0.06942408242694142 1105 12-13 0.1189101011107212 1105 14-15 -0.07551498338380469 1105 16-17 -0.07152123658267584 1105 18-19 -0.23610737585335784 1105 20-21 -0.34688365758074013 1105 22-23 0.17630185647820795 1105 24-25 0.187466958340206 1105 26-27 -0.29063624296722423 1105 28-29 0.0012468611390765716 1105 30-31 0.3546066274069908 1105 32-33 0.10567540679994991 1105 34-35 0.0824967395895122 1105 36-37 0.43080790627644205 1105 38-39 0.2495627902690991 1105 40-41 0.3128561686909741 1105 42-43 0.14173697799074958 1105 44-45 -0.4217850389259361 1105 46-47 -0.26488079037176604 1105 48-49 -0.37303526625152017 1105 50-51 -0.43972563757254335 1105 52-53 -0.1020166319313951 1105 54-55 -0.2916428980938477 1105 56-57 -0.31146622941406577 1105 58-59 -0.3199320988822798 1105 60-61 -0.1597196183346199 1105 62-63 -0.37903891313224136 1105 64-65 -0.1720347581535684 1105 66-67 -0.15527530286113134 1105 68-69 0.08522559533586715 1105 70-71 0.0612673853682395 1105 72-73 -0.08330500241220662 1105 74-75 -0.4850020246713882 1105 76-77 -0.6863279750336275 1106 1 -0.17913436513147474 1106 2 -0.286514051913926 1106 3 -0.44845973746823375 1106 4 -0.12572026255366353 1106 5 -0.10874359158469105 1106 6 -0.42554708150657206 1106 7 -0.5538355150004932 1106 8 -0.32814106429814416 1106 9 -0.21607867385912272 1106 10-11 -0.8150340082314145 1106 12-13 -0.1254804553212736 1106 14-15 -0.13922186627612376 1106 16-17 -0.2140772276819618 1106 18-19 0.0029165775212547373 1106 20-21 0.036139551771896095 1106 22-23 0.23027100712199555 1106 24-25 -0.10645272861943411 1106 26-27 -0.1791018353947358 1106 28-29 0.09957143384311351 1106 30-31 0.21747579665132477 1106 32-33 0.08717130434536813 1106 34-35 0.2843299271037196 1106 36-37 0.26385419408894606 1106 38-39 0.614188678100895 1106 40-41 0.6113881030792534 1106 42-43 0.6373072915133484 1106 44-45 0.6103698003170912 1106 46-47 0.5748341389805915 1106 48-49 0.514448630494762 1106 50-51 0.37952868705527365 1106 52-53 0.1750867802361178 1106 54-55 0.2678616383735637 1106 56-57 0.5497116676215583 1106 58-59 0.09176351345470835 1106 60-61 0.04492776346577543 1106 62-63 -0.09938998128603416 1106 64-65 0.05498839608632622 1106 66-67 -0.2742671530647023 1106 68-69 0.11729776546889781 1106 70-71 0.4653436614003965 1106 72-73 0.14747615178634987 1106 74-75 -0.09383246343768903 1106 76-77 -0.31605303135195584 1107 1 0.29673055238387036 1107 2 0.24225693913482615 1107 3 0.3496809672884993 1107 4 0.22906236758467458 1107 5 0.18359606269489603 1107 6 -0.29066803433374133 1107 7 0.22994127093396344 1107 8 0.5174067373441531 1107 9 0.5875733370895091 1107 10-11 0.5327238641824579 1107 12-13 0.44781251930452726 1107 14-15 0.4466934790744048 1107 16-17 0.4368271443743552 1107 18-19 0.6514197708731686 1107 20-21 0.5555924899022457 1107 22-23 0.7718369825122231 1107 24-25 0.9744414099153254 1107 26-27 0.8260580787916432 1107 28-29 0.7737786441604761 1107 30-31 0.9096687293566745 1107 32-33 0.7502942545586677 1107 34-35 0.8069330637397272 1107 36-37 0.743831014546128 1107 38-39 0.8180823481129771 1107 40-41 0.7080301517562937 1107 42-43 0.39789836985527316 1107 44-45 0.7488336624234719 1107 46-47 0.7352137656419977 1107 48-49 0.2902019656364274 1107 50-51 0.6684037394558366 1107 52-53 0.7253789904301868 1107 54-55 0.6719377856376347 1107 56-57 0.9365541946849056 1107 58-59 0.888030127515318 1107 60-61 0.42657647009660593 1107 62-63 0.4968883458414304 1107 64-65 0.5406522187381633 1107 66-67 0.8598567095348564 1107 68-69 0.6071351387828336 1107 70-71 1.029713128517507 1107 72-73 0.6214423195174668 1107 74-75 0.9481773755384637 1107 76-77 0.9088594288852647 1108 1 0.503611448871446 1108 2 0.6017488999799312 1108 3 0.6395556184821984 1108 4 0.6641733212100434 1108 5 0.5419585554973025 1108 6 0.3293767014593243 1108 7 0.2925049419265662 1108 8 0.14920015542449505 1108 9 0.08783909427602765 1108 10-11 0.09074197996400812 1108 12-13 -0.018923539516400467 1108 14-15 -0.043434748768085285 1108 16-17 -0.1330614590716337 1108 18-19 -0.4748591855869435 1108 20-21 -0.4149915115151259 1108 22-23 -0.9123936504328078 1108 24-25 -0.9239507591062441 1108 26-27 -1.0646824979014546 1108 28-29 -0.9826604310045148 1108 30-31 -1.0106594807686733 1108 32-33 -1.0142836344434158 1108 34-35 -0.9718849811732397 1108 36-37 -0.7438112762808231 1108 38-39 -0.6802566694712873 1108 40-41 -0.8012436667315477 1108 42-43 -0.5131947780386028 1108 44-45 -0.3387167207098081 1108 46-47 -0.5369935692563494 1108 48-49 -0.49590225547354905 1108 50-51 -0.6440491993757185 1108 52-53 -0.07261232816838614 1108 54-55 -0.12697260989764203 1108 56-57 -0.31683974605124376 1108 58-59 -0.4293946853473294 1108 60-61 -0.2550353472396232 1108 62-63 -0.006106959114941191 1108 64-65 0.28316118804320567 1108 66-67 0.283302029993731 1108 68-69 -0.42339925462638917 1108 70-71 -0.29664223313373483 1108 72-73 -0.054677182630737065 1108 74-75 -0.08623264350746851 1108 76-77 0.11400736705242664 1109 1 0.6414846283781444 1109 2 0.4616386613987302 1109 3 0.4885944902664221 1109 4 0.767377850678109 1109 5 0.28168711028795457 1109 6 0.732122287880145 1109 7 0.4549338807072587 1109 8 0.5666711255470744 1109 9 0.47277469234339264 1109 10-11 0.5463986259897666 1109 12-13 0.33026812109637405 1109 14-15 0.6605296889113674 1109 16-17 0.9776297229949975 1109 18-19 1.201531192752789 1109 20-21 0.7342341662330512 1109 22-23 0.921605611568328 1109 24-25 0.6540580276928019 1109 26-27 0.7375856955003748 1109 28-29 0.8142515049740844 1109 30-31 0.9830934741096158 1109 32-33 0.7214695279970513 1109 34-35 0.891588110696091 1109 36-37 1.0717524280122568 1109 38-39 1.0796054169898888 1109 40-41 1.0646682807782852 1109 42-43 0.9537855381477875 1109 44-45 1.0750354269822182 1109 46-47 0.921825517239423 1109 48-49 0.9007000669166416 1109 50-51 0.8316083691486291 1109 52-53 0.8246126807683147 1109 54-55 0.6296088409620104 1109 56-57 0.6734365422756952 1109 58-59 0.7792687295118164 1109 60-61 0.8209871847883754 1109 62-63 0.8194802688921143 1109 64-65 0.9282269737237243 1109 66-67 1.0203582216889373 1109 68-69 1.0412171589297294 1109 70-71 1.0612324454331628 1109 72-73 1.2031611725542817 1109 74-75 1.223579858654638 1109 76-77 1.3031138118581822 1110 1 -0.29067410977103236 1110 2 -0.336263847200982 1110 3 -0.38644536207018376 1110 4 -0.16454596843423275 1110 5 -0.1901981149826213 1110 6 -0.25450410695163583 1110 7 -0.009450715315900027 1110 8 -0.2624084952264809 1110 9 -0.4920377919254193 1110 10-11 -0.2760445319932856 1110 12-13 -0.4612915735088805 1110 14-15 -0.14704645002645833 1110 16-17 -0.17870889601666562 1110 18-19 -0.3985694868344254 1110 20-21 -0.4311488356966393 1110 22-23 -0.03612574173312666 1110 24-25 -0.13519442637213785 1110 26-27 -0.12748687770207212 1110 28-29 -0.12046617268343951 1110 30-31 0.07562640472459847 1110 32-33 0.30606555115335965 1110 34-35 -0.04838168542774568 1110 36-37 -0.04716694441831493 1110 38-39 -0.011383759954483708 1110 40-41 0.27408182218276167 1110 42-43 0.0942083924140853 1110 44-45 0.06344049452144063 1110 46-47 0.14942850406717767 1110 48-49 0.17953218370496415 1110 50-51 0.2959845752061874 1110 52-53 7.683985298747587E-4 1110 54-55 0.41254365092929035 1110 56-57 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-0.9768971954113113 2314 2 -0.5859412217933624 2314 3 -0.6186523973855245 2314 4 -0.39788623994837735 2314 5 -0.5963499686332625 2314 6 -0.5564167321279783 2314 7 -0.8014903092010499 2314 8 -0.9928416689816544 2314 9 -0.9170337446532812 2314 10-11 -1.0544228478655313 2314 12-13 -0.8432939324903614 2314 14-15 -1.0968834027774506 2314 16-17 -1.0749044602064046 2314 18-19 -1.2357396341551308 2314 20-21 -0.854882039517264 2314 22-23 -0.9101121891532813 2314 24-25 -0.8939746941859177 2314 26-27 -1.178440877563311 2314 28-29 -1.1060023784236286 2314 30-31 -1.1092391102040544 2314 32-33 -1.2215437830125566 2314 34-35 -1.4333003930759318 2314 36-37 -2.0619475822684024 2314 38-39 -1.5140318322965847 2314 40-41 -1.3563444133916 2314 42-43 -1.1835200471890523 2314 44-45 -1.3052266809451503 2314 46-47 -0.980033324374741 2314 48-49 -0.7641968572071747 2314 50-51 -0.7403807431406761 2314 52-53 -0.666166544461408 2314 54-55 -0.5260256961878973 2314 56-57 -0.760184649595729 2314 58-59 -0.6482623939687251 2314 60-61 -0.685873933258101 2314 62-63 -0.69720377698901 2314 64-65 -0.6995812380774957 2314 66-67 -0.9248115805796608 2314 68-69 -1.0036628265794327 2314 70-71 -1.174909466867895 2314 72-73 -1.2735115156430261 2314 74-75 -1.1675332433148427 2314 76-77 -0.9761163134190269 2315 1 -0.7089086818054042 2315 2 -0.26595919370248566 2315 3 -0.29156755825364655 2315 4 -0.3679161280146417 2315 5 -0.4150837304273267 2315 6 -0.34125781747501804 2315 7 -0.827975433539315 2315 8 -0.6543290398085588 2315 9 -0.6223803876178664 2315 10-11 -0.6464273017734428 2315 12-13 -0.5760555507435683 2315 14-15 -0.7846761788513703 2315 16-17 -0.5599721478858797 2315 18-19 -0.8447920066424857 2315 20-21 -0.7196434233542632 2315 22-23 -0.5731974971175724 2315 24-25 -0.8920407604866334 2315 26-27 -0.661433981819922 2315 28-29 -0.6875792181024778 2315 30-31 -0.7510335662607837 2315 32-33 -0.6575657399971817 2315 34-35 -0.9909550589351674 2315 36-37 -0.8476579610459254 2315 38-39 -0.5793226060654071 2315 40-41 -0.7147921874143783 2315 42-43 -0.6866084807027555 2315 44-45 -0.6502521737075533 2315 46-47 -0.788792676604622 2315 48-49 -0.9832509878160707 2315 50-51 -0.8356748378057546 2315 52-53 -1.160720274609286 2315 54-55 -0.9095520973130924 2315 56-57 -0.856615662859781 2315 58-59 -0.635615639656443 2315 60-61 -0.8753017623686894 2315 62-63 -0.7597557880847141 2315 64-65 -0.3339349330629453 2315 66-67 -0.29269069995611474 2315 68-69 -0.7956028160307049 2315 70-71 -0.9130665647952618 2315 72-73 -0.7403533433080476 2315 74-75 -0.766279116612882 2315 76-77 -0.43104393741543134 2316 1 -1.8883011027261674 2316 2 -1.6312582854657194 2316 3 -1.7231157365603593 2316 4 -2.7952467470771722 2316 5 -2.5922003396767295 2316 6 -2.330588645327609 2316 7 -2.090800363072667 2316 8 -1.9391604418338204 2316 9 -2.3762795420181178 2316 10-11 -2.030141676969187 2316 12-13 -2.805192203838878 2316 14-15 -3.930954496003473 2316 16-17 -2.7455238756236326 2316 18-19 -2.3578205064858935 2316 20-21 -2.3450531738501432 2316 22-23 -2.6254472100312363 2316 24-25 -2.653696465675065 2316 26-27 -2.337015984116608 2316 28-29 -2.536780596116884 2316 30-31 -2.748214900429275 2316 32-33 -2.8023512082088935 2316 34-35 -2.5624343365215516 2316 36-37 -2.8747902295499443 2316 38-39 -2.854654060288187 2316 40-41 -2.4343349371794893 2316 42-43 -2.7982276476304087 2316 44-45 -2.491843153976891 2316 46-47 -2.4496445400559246 2316 48-49 -2.143048461456324 2316 50-51 -2.4353094551979666 2316 52-53 -2.45514557996475 2316 54-55 -2.743407405800408 2316 56-57 -2.983340790743693 2316 58-59 -2.8487902925429687 2316 60-61 -2.3844676460726255 2316 62-63 -2.3171730550228062 2316 64-65 -2.544844213781179 2316 66-67 -2.3988082487607905 2316 68-69 -2.9737738150911497 2316 70-71 -2.9316801987862853 2316 72-73 -2.7785828035857385 2316 74-75 -2.9639093494138002 2316 76-77 -3.0236423297319597 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9094.0 3 1608.0 4 2930.0 5 3709.0 6 3620.0 7 3428.0 8 4323.0 9 4974.0 10 7538.0 11 9085.0 12 5698.0 13 3271.0 14 2943.0 15 2789.0 16 2910.0 17 3167.0 18 3228.0 19 3432.0 20 3534.0 21 3714.0 22 4082.0 23 4585.0 24 5176.0 25 5881.0 26 6744.0 27 7607.0 28 8725.0 29 10031.0 30 11539.0 31 13834.0 32 16980.0 33 22508.0 34 45712.0 35 48447.0 36 33050.0 37 38228.0 38 33215.0 39 9772.0 40 112.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 15.483066118375458 29.555198350262756 40.79306059817566 14.168674933186129 2 16.399430913092647 29.35807483249632 40.84998236803386 13.392511886377179 3 14.974268675882598 30.328913447413736 40.464088021557885 14.232729855145779 4 15.27454485752855 30.479526189694383 40.94786384034441 13.298065112432656 5 14.885554666465609 30.31907519512011 40.84108989996522 13.95428023844906 6 14.263373204461546 31.825235067784163 41.071558865448324 12.839832862305975 7 15.538070510403873 31.25004560434883 39.84482360237872 13.367060282868573 8 14.02243550764471 31.830741315483518 40.15308877397637 13.993734402895406 9 14.3269235446634 32.038808975974234 39.943620452301154 13.690647027061209 10-11 13.988896253549733 32.27237623822583 39.95974435843331 13.778983149791122 12-13 14.689428378927296 32.40977892583593 39.13759428305957 13.7631984121772 14-15 15.065164997920654 32.52072657939088 38.82236062910549 13.591747793582968 16-17 14.179422983622384 33.36259716951422 38.738428726548904 13.719551120314494 18-19 14.250466653290287 33.56717679319507 38.19163489776189 13.990721655752756 20-21 14.541196327187253 33.70186303681933 37.43717829144409 14.31976234454933 22-23 14.32242200781685 34.22620712466832 36.490759139319444 14.960611728195387 24-25 14.53193313591117 34.76142119632965 35.385186040996516 15.321459626762662 26-27 14.847702155809792 35.29725075881392 34.53563993306872 15.319407152307573 28-29 14.429877040409348 35.648796669195896 34.11009941827662 15.811226872118134 30-31 14.2634271379637 35.800472529223 33.83897770260329 16.097122630210013 32-33 14.903151041571666 35.78006306217571 33.20504448765326 16.111741408599364 34-35 14.687194852793645 35.15709266990049 34.02168665615207 16.13402582115379 36-37 15.2164766426056 35.41573438138413 33.77507442068603 15.59271455532424 38-39 15.600335648007393 35.33291174646414 33.995670627151 15.071081978377457 40-41 15.301006293132277 35.50530508010823 34.32718207521357 14.866506551545921 42-43 15.429142638943274 35.51429259382669 33.92937491031407 15.12718985691597 44-45 15.585625354945648 35.629550448797225 33.8337534916923 14.951070704564827 46-47 14.921002451648052 35.741769467252986 33.87786025606102 15.459367825037942 48-49 15.023129344352403 35.56775122456624 33.55384443266223 15.85527499841913 50-51 14.80610774292021 35.684931923355244 33.745826922959985 15.763133410764564 52-53 14.171578273952143 35.62020038871226 34.65219009440502 15.556031242930569 54-55 13.993770546433268 35.50837181640504 34.8000481621166 15.69780947504509 56-57 13.604579328326263 36.03801027357279 34.447965715842315 15.90944468225863 58-59 13.582041088343711 35.82101895103475 33.70299789486998 16.893942065751567 60-61 13.918106892286811 35.21532543565532 33.749256628321454 17.117311043736404 62-63 13.988501734956113 35.00038311333709 33.965733856637776 17.045381295069028 64-65 14.20626397829389 34.359573709922806 34.47705522725863 16.957107084524665 66-67 14.003407693701606 34.80724396387018 34.2452542210039 16.944094121424325 68-69 13.524262669532188 34.85694894969405 34.39647088443168 17.222317496342082 70-71 13.421255278175511 34.69563281537973 34.520453956548344 17.362657949896413 72-73 13.929231469281037 34.13803549984852 34.099588888929446 17.83314414194099 74-75 14.062539925508888 33.702909193118394 34.109921237998016 18.124629643374707 76-77 14.002871535297984 33.56021706860049 34.867982381427495 17.56892901467403 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 83681.0 1 46480.0 2 7819.5 3 5695.0 4 5030.0 5 4702.5 6 3945.0 7 3455.5 8 3095.0 9 2794.0 10 2652.0 11 2369.0 12 2266.0 13 2304.0 14 2361.0 15 2368.5 16 2351.5 17 2384.0 18 2528.5 19 2795.0 20 2989.5 21 3345.0 22 3628.0 23 3796.0 24 4185.5 25 4765.5 26 5124.0 27 5393.0 28 6001.5 29 6747.0 30 7153.0 31 7439.0 32 8282.0 33 9105.0 34 9346.5 35 9322.0 36 9608.5 37 10089.0 38 9997.0 39 9711.0 40 9685.5 41 9505.0 42 9315.0 43 9280.0 44 9394.0 45 9724.5 46 10318.0 47 12242.5 48 13790.0 49 13197.5 50 14012.5 51 12313.5 52 9207.0 53 8765.0 54 7725.0 55 6825.5 56 6372.0 57 6220.0 58 6475.5 59 6130.0 60 5301.0 61 5073.0 62 4659.5 63 3330.0 64 1926.5 65 1439.0 66 1295.5 67 1048.5 68 850.5 69 653.5 70 551.0 71 457.0 72 364.0 73 332.5 74 300.0 75 309.0 76 238.0 77 131.5 78 105.0 79 97.5 80 101.5 81 98.0 82 65.5 83 48.0 84 44.0 85 30.0 86 16.0 87 12.0 88 10.5 89 7.5 90 6.5 91 7.0 92 6.0 93 6.0 94 6.0 95 3.5 96 2.0 97 1.5 98 2.0 99 5.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0014590623578934058 2 0.009240728266658236 3 0.012402030042093951 4 0.021399581249103284 5 0.016049685936827464 6 0.015806508877178563 7 0.018967810652614277 8 0.021642758308752185 9 0.019697341831560976 10-11 0.024439294494714544 12-13 0.02285864360699669 14-15 0.008754374147360435 16-17 0.0052283067824513705 18-19 0.012280441512269499 20-21 0.009605493856131587 22-23 0.01459062357893406 24-25 0.022007523898225537 26-27 0.013374738280689554 28-29 0.007538488849115931 30-31 0.00766007737894038 32-33 0.01009184797542939 34-35 0.011794087392971697 36-37 0.011429321803498345 38-39 0.018967810652614277 40-41 0.014712212108758508 42-43 0.016049685936827464 44-45 0.016657628585949717 46-47 0.01726557123507197 48-49 0.013861092399987356 50-51 0.02565517979295905 52-53 0.029667601277165917 54-55 0.02711424215085246 56-57 0.01726557123507197 58-59 0.02054846154033213 60-61 0.021764346838576636 62-63 0.028573304508745866 64-65 0.02298023213682114 66-67 0.02298023213682114 68-69 0.031248252164883774 70-71 0.05216147929468926 72-73 0.0640771552174854 74-75 0.07331788348414364 76-77 0.07125087847712798 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 411223.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.04037271786025 #Duplication Level Percentage of deduplicated Percentage of total 1 82.39864673930255 38.760630540640925 2 9.413288832578424 8.856092303707213 3 3.4034809256485525 4.803030338419079 4 1.6189145981991282 3.046173843906878 5 0.8919467017916177 2.0978752648371923 6 0.5324168271179642 1.502705159333376 7 0.34962823293117623 1.1512649672838573 8 0.25479538002950824 0.9588535714701532 9 0.15700112630485916 0.6646852348653984 >10 0.8782325830758888 7.37101009742637 >50 0.0531459363507217 1.7174789256675798 >100 0.04179437713017904 3.5410260099723354 >500 0.0046438196811310044 1.4550997964071664 >1k 0.0010319599291402234 0.7031566489060153 >5k 0.0 0.0 >10k+ 0.0010319599291402234 23.370917297156506 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 53940 13.11697059746172 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 42348 10.298062122011657 No Hit CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA 1459 0.3547953300277465 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 1438 0.34968861177511956 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 917 0.22299336369804218 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 848 0.20621414658226803 No Hit AGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGC 727 0.17678972236475102 No Hit AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG 706 0.1716830041121241 No Hit TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 628 0.1527151934595098 No Hit CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG 568 0.13812456988057575 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 548 0.13326102868759773 No Hit TACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT 535 0.130099726912162 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 518 0.1259657168981307 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 445 0.10821379154376093 No Hit TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 437 0.10626837506656972 No Hit GACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTAC 419 0.10189118799288951 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.4317705964890096E-4 0.0 2 0.0 0.0 0.0 0.0024317705964890094 0.0 3 0.0 0.0 0.0 0.0031613017754357126 0.0 4 0.0 0.0 0.0 0.005593072371924722 0.0 5 0.0 0.0 0.0 0.010456613564902741 0.0 6 0.0 0.0 0.0 0.019454164771912075 0.0 7 0.0 0.0 0.0 0.031126663635059323 0.0 8 0.0 0.0 0.0 0.04279916249820657 0.0 9 0.0 0.0 0.0 0.05228306782451371 0.0 10 0.0 0.0 0.0 0.06638733728414996 0.0 11 0.0 0.0 0.0 0.08657103323500874 0.0 12 0.0 0.0 0.0 0.10359342741043182 0.0 13 0.0 0.0 0.0 0.1254793627788329 0.0 14 0.0 0.0 0.0 0.14906753756477628 0.0 15 0.0 0.0 0.0 0.1789783159015911 0.0 16 0.0 0.0 0.0 0.21594122896822404 0.0 17 0.0 0.0 0.0 0.24560883024538996 0.0 18 0.0 0.0 0.0 0.2730878379857158 0.0 19 0.0 0.0 0.0 0.29740554395060587 0.0 20 0.0 0.0 0.0 0.3219664269751449 0.0 21 0.0 0.0 0.0 0.3509044970733641 0.0 22 0.0 0.0 0.0 0.3822743377680723 0.0 23 0.0 0.0 0.0 0.41923725083470526 0.0 24 0.0 0.0 0.0 0.4525525080066047 0.0 25 0.0 0.0 0.0 0.49024495225218434 0.0 26 0.0 0.0 0.0 0.5332872918100398 0.0 27 0.0 0.0 0.0 0.5702502048766728 0.0 28 0.0 0.0 0.0 0.6140220756134749 0.0 29 0.0 0.0 0.0 0.6551189986941391 0.0 30 0.0 0.0 0.0 0.6981613382519947 0.0 31 0.0 0.0 0.0 0.7429059172273924 0.0 32 0.0 0.0 0.0 0.7874073191431413 0.0 33 0.0 0.0 0.0 0.8333677834167836 0.0 34 0.0 0.0 0.0 0.8824895494658616 0.0 35 0.0 0.0 0.0 0.9359885025886198 0.0 36 0.0 0.0 0.0 1.0035917251710142 0.0 37 0.0 0.0 0.0 1.0741130724691956 0.0 38 0.0 0.0 0.0 1.1375822850375588 0.0 39 0.0 0.0 0.0 1.2000787893673261 0.0 40 0.0 0.0 0.0 1.2581981066234136 0.0 41 0.0 0.0 0.0 1.3228832044900212 0.0 42 0.0 0.0 0.0 1.3960794994443404 0.0 43 0.0 0.0 0.0 1.4738961585319887 0.0 44 0.0 0.0 0.0 1.5580354211705085 0.0 45 0.0 0.0 0.0 1.6438769232265704 0.0 46 0.0 0.0 0.0 1.7523338918299802 0.0 47 0.0 0.0 0.0 1.8435252891983183 0.0 48 0.0 0.0 0.0 1.947605070728048 0.0 49 0.0 0.0 0.0 2.045605425766555 0.0 50 0.0 0.0 0.0 2.1445784890436577 0.0 51 0.0 0.0 0.0 2.256439936482152 0.0 52 0.0 0.0 0.0 2.364410550966264 0.0 53 0.0 0.0 0.0 2.4740834048679186 0.0 54 0.0 0.0 0.0 2.5968878199906134 0.0 55 0.0 0.0 0.0 2.7138559856817346 0.0 56 0.0 0.0 0.0 2.84760336848863 0.0 57 0.0 0.0 0.0 2.9937527813376197 0.0 58 0.0 0.0 0.0 3.143063495962045 0.0 59 0.0 0.0 0.0 3.316935093611009 0.0 60 0.0 0.0 0.0 3.4728115888459548 0.0 61 0.0 0.0 0.0 3.631606208796687 0.0 62 0.0 0.0 0.0 3.8047482752667046 0.0 63 0.0 0.0 0.0 3.9715677381858505 0.0 64 0.0 0.0 0.0 4.14860063761025 0.0 65 0.0 0.0 0.0 4.321985881139917 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGTAG 40 0.0022568437 35.494465 2 TTTAGTC 50 0.006720109 28.402481 14 ATTTGCG 110 2.6179576E-5 22.587389 3 AGGTCTC 100 3.5580387E-4 21.309639 62 TAGCCGT 285 0.0 21.172136 24 GTAGCCG 280 0.0 20.282553 23 GAGGTGT 105 4.9658574E-4 20.28255 54 GCGCAAG 90 0.004857087 19.721546 38 GGTAGCC 295 0.0 19.251238 22 GTGGTAG 300 1.8189894E-12 18.934988 20 AGCCGTT 300 1.8189894E-12 18.930382 25 CTCGGTG 95 0.006619537 18.692665 70 TGAGGTG 115 9.1526884E-4 18.521105 53 TGCGCGC 135 1.2648193E-4 18.406776 6 TTGCGCG 135 1.2648193E-4 18.406776 5 CGCGCCT 155 1.752591E-5 18.321953 8 GATGTGG 320 3.6379788E-12 17.75155 17 TGTGGTA 320 3.6379788E-12 17.75155 19 TTGAGGC 100 0.008922629 17.749393 60 TTGAGGT 120 0.0012178947 17.749392 52 >>END_MODULE