FastQCFastQC Report
Thu 26 May 2016
SRR936153_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936153_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2145545
Sequences flagged as poor quality0
Sequence length77
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119290.5559892707913374No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46780.2180331803807424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAG3500.021.29593738
TGCGTAA3600.020.70486637
TATACGG3150.020.28231812
TTGCGTA3550.019.99618536
CGGTATA1309.504378E-519.11173810
TCGAACT5800.018.97538424
TATAGCG950.00664593418.6841343
CTCGAAC5750.018.52338823
CGTAAGT3850.018.438939
ATACGTA3300.018.2839641
TACGTAG3350.018.010652
AGCGACG1200.001218400817.75323546
CGAACTC5900.017.44785925
CGGTTAC2651.5661499E-917.4109614
CGATCGT1854.1613566E-617.2665731
CGCGGTA1452.1893128E-417.13865719
ATTCGCG1905.3548283E-616.81532556
ATATACG3701.8189894E-1216.307711
GCCTCGA6800.016.18488521
CTTGCGT4400.016.1329135
Produced by FastQC (version 0.11.5)