##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936153_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2145545 Sequences flagged as poor quality 0 Sequence length 77 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.72261313559026 40.0 36.0 41.0 30.0 41.0 2 36.582548956092744 39.0 36.0 41.0 30.0 41.0 3 36.49237466471223 39.0 36.0 41.0 30.0 41.0 4 36.372458279831 39.0 36.0 41.0 30.0 41.0 5 36.29703734948463 39.0 36.0 41.0 30.0 41.0 6 36.17944484967689 39.0 35.0 41.0 29.0 41.0 7 35.9993232488715 39.0 35.0 40.0 29.0 41.0 8 35.795878436481175 39.0 35.0 40.0 28.0 41.0 9 35.688805408416044 38.0 35.0 40.0 28.0 41.0 10-11 35.87965738308914 39.0 35.0 40.5 29.0 41.0 12-13 36.01789941483399 39.0 35.0 41.0 29.0 41.0 14-15 35.94904814394478 39.0 35.0 41.0 28.5 41.0 16-17 35.76637544306924 39.0 35.0 41.0 27.5 41.0 18-19 35.58016914117392 39.0 35.0 41.0 27.0 41.0 20-21 35.3262648884083 39.0 35.0 41.0 26.0 41.0 22-23 34.988891633594264 39.0 35.0 41.0 24.0 41.0 24-25 34.70744752498783 39.0 34.0 41.0 23.5 41.0 26-27 33.768397073936924 38.0 33.0 40.0 21.5 40.5 28-29 33.79904476484995 38.0 33.0 40.0 22.0 40.5 30-31 34.172339661950694 38.0 34.0 40.0 21.5 41.0 32-33 34.02614254187165 38.0 34.0 40.0 20.5 41.0 34-35 33.772054885821554 38.0 33.0 40.0 18.5 41.0 36-37 33.49668499145905 38.0 33.0 40.0 18.0 41.0 38-39 33.231086274116834 37.0 33.0 40.0 16.0 41.0 40-41 32.870215959115285 37.0 32.0 40.0 13.0 41.0 42-43 32.51683837905986 36.0 32.0 39.5 9.0 41.0 44-45 32.149881731681226 36.0 32.0 39.0 7.5 41.0 46-47 31.772165347266082 35.0 31.0 39.0 4.5 40.0 48-49 31.319731816391638 35.0 31.0 38.0 2.0 40.0 50-51 30.887590099485212 35.0 31.0 37.0 2.0 39.5 52-53 30.440838807855346 35.0 30.0 37.0 2.0 39.0 54-55 29.986578701448813 35.0 30.0 36.0 2.0 39.0 56-57 29.532821497568214 34.0 29.0 36.0 2.0 37.5 58-59 29.108334013036313 34.0 29.0 35.0 2.0 37.0 60-61 28.75699530888422 34.0 29.0 35.0 2.0 36.5 62-63 28.417594830217965 34.0 29.0 35.0 2.0 36.0 64-65 28.049823238384654 34.0 28.5 35.0 2.0 36.0 66-67 27.67774574758395 34.0 27.0 35.0 2.0 35.5 68-69 27.327448503760117 34.0 26.5 35.0 2.0 35.0 70-71 26.917396978390105 33.5 25.5 35.0 2.0 35.0 72-73 26.460825571125284 33.0 25.0 35.0 2.0 35.0 74-75 25.910986952033166 33.0 24.0 35.0 2.0 35.0 76-77 24.254663733457 31.0 11.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7512451625267147 1101 2 0.7505338486233413 1101 3 0.7383278164317062 1101 4 0.8221492882159822 1101 5 0.7898730058769488 1101 6 0.6565675571382315 1101 7 0.7492248256543448 1101 8 0.8748588480031572 1101 9 0.7950745893893583 1101 10-11 0.8258489518817527 1101 12-13 0.9135792041894533 1101 14-15 0.883260618898575 1101 16-17 0.9311416391188061 1101 18-19 0.9758929369454705 1101 20-21 0.8531374077841747 1101 22-23 0.8070075150712199 1101 24-25 0.9144895817572092 1101 26-27 0.7402472000425604 1101 28-29 0.7456518524456186 1101 30-31 0.8961530566908564 1101 32-33 0.9995721491100795 1101 34-35 0.9840906068871007 1101 36-37 0.8674466729838457 1101 38-39 0.8299829741308642 1101 40-41 0.7338782972750479 1101 42-43 0.692282229488022 1101 44-45 0.6242184008000677 1101 46-47 0.5491224244822099 1101 48-49 0.7648222031985661 1101 50-51 0.8153163476079079 1101 52-53 0.8089336584479625 1101 54-55 0.5378127838395912 1101 56-57 0.6548496247116056 1101 58-59 0.6438119103918005 1101 60-61 0.6385884047320509 1101 62-63 0.7524986628962864 1101 64-65 0.7041426006924532 1101 66-67 0.7278322414276346 1101 68-69 0.7194809587281341 1101 70-71 0.6646522938063306 1101 72-73 0.7026096785902034 1101 74-75 0.690325772338344 1101 76-77 0.605078370012766 1102 1 0.7219765619049099 1102 2 0.7852178843625097 1102 3 0.8371786710198279 1102 4 0.7645184170794366 1102 5 0.740250305238682 1102 6 0.7760971673035542 1102 7 0.7154155562871765 1102 8 0.8992418333463092 1102 9 0.899742189440758 1102 10-11 0.7910657158323602 1102 12-13 0.9904447900236519 1102 14-15 0.813476582483041 1102 16-17 0.740540643057507 1102 18-19 0.7937498946072239 1102 20-21 0.8989217327824264 1102 22-23 0.8379985938069581 1102 24-25 0.8588449713776996 1102 26-27 0.7468755844076895 1102 28-29 0.8401649480138431 1102 30-31 0.9807314666451603 1102 32-33 0.9050691990281194 1102 34-35 1.0614724852449768 1102 36-37 1.043898097522849 1102 38-39 1.0961035881807888 1102 40-41 1.1446352181876023 1102 42-43 1.0353214113560014 1102 44-45 1.1062845628978906 1102 46-47 1.0123247457694653 1102 48-49 1.0295799402591221 1102 50-51 1.0559109181945665 1102 52-53 0.9017862760961464 1102 54-55 0.9730552722342836 1102 56-57 0.9252189429574749 1102 58-59 0.893873346947565 1102 60-61 0.9531480793550351 1102 62-63 0.943964279843307 1102 64-65 0.8208641657126172 1102 66-67 0.9227292872874884 1102 68-69 0.9925129034825773 1102 70-71 1.136211113953916 1102 72-73 1.2571360309271604 1102 74-75 1.226778504517796 1102 76-77 1.2656938177556185 1103 1 0.5364282423299542 1103 2 0.24131025453424115 1103 3 0.338701732265271 1103 4 0.3768208360001495 1103 5 0.4069139673459361 1103 6 0.45865845405620576 1103 7 0.5181847585516124 1103 8 0.5745220711071113 1103 9 0.5621619113687828 1103 10-11 0.4646873116039387 1103 12-13 0.606314246924498 1103 14-15 0.6398614965901999 1103 16-17 0.6302751902328438 1103 18-19 0.6137890540316562 1103 20-21 0.6922325819961017 1103 22-23 0.7050867751220977 1103 24-25 0.6568771101482866 1103 26-27 0.4839463392782051 1103 28-29 0.598379037895242 1103 30-31 0.7832900993703191 1103 32-33 0.7322028743804694 1103 34-35 0.7733928243595756 1103 36-37 0.6765376280103652 1103 38-39 0.7309350699543771 1103 40-41 0.8621973687510831 1103 42-43 0.7976836710851458 1103 44-45 0.8027082608595464 1103 46-47 0.47758828318749025 1103 48-49 0.4454159714364323 1103 50-51 0.618216216484722 1103 52-53 0.6891920393418722 1103 54-55 0.7151726854087741 1103 56-57 0.6316260407283458 1103 58-59 0.6916587362758726 1103 60-61 0.527821270923237 1103 62-63 0.4729975878882655 1103 64-65 0.5477149117196838 1103 66-67 0.6163067911495972 1103 68-69 0.5387851215735324 1103 70-71 0.4485137832545796 1103 72-73 0.3592940557297979 1103 74-75 0.40734589052606296 1103 76-77 0.47020553929305464 1104 1 0.5120217906694009 1104 2 0.5719657367468756 1104 3 0.5216167975475869 1104 4 0.4882011082580604 1104 5 0.5739977941231302 1104 6 0.5205391461004538 1104 7 0.5948759065995404 1104 8 0.6662112864144163 1104 9 0.7010991256124655 1104 10-11 0.648481712318933 1104 12-13 0.8568531984188681 1104 14-15 0.7216039915244536 1104 16-17 0.7928956172084938 1104 18-19 0.7367896523195157 1104 20-21 0.7541999396072825 1104 22-23 0.8433827380331707 1104 24-25 0.7940885224277139 1104 26-27 0.5589571109218241 1104 28-29 0.6678513381520332 1104 30-31 0.8753011098366628 1104 32-33 0.7860138203400595 1104 34-35 1.0360227303816103 1104 36-37 0.8362031777088816 1104 38-39 0.9042225017237087 1104 40-41 0.9641367570201993 1104 42-43 0.9732431194785036 1104 44-45 0.8026263963680549 1104 46-47 0.70647032199496 1104 48-49 0.8125160480074278 1104 50-51 0.7256928450956828 1104 52-53 0.8259043859844439 1104 54-55 0.8213515463967163 1104 56-57 0.8027298449035563 1104 58-59 0.8232286387032453 1104 60-61 0.5052307337198307 1104 62-63 0.47358085700208363 1104 64-65 0.5472894625729232 1104 66-67 0.6023282753466717 1104 68-69 0.6918448838833662 1104 70-71 0.6945331992960782 1104 72-73 0.6821292015716978 1104 74-75 0.6326294617383219 1104 76-77 0.7414622392553838 1105 1 -0.03063225470776132 1105 2 0.2871185762987096 1105 3 0.12979386608612486 1105 4 0.09712860050360206 1105 5 0.20625014238454753 1105 6 0.024650867485213723 1105 7 0.1842578281799092 1105 8 0.3287000622300411 1105 9 0.07267801327911627 1105 10-11 0.3133579507920956 1105 12-13 0.46263720412294873 1105 14-15 0.08904613573925246 1105 16-17 0.3353130253226979 1105 18-19 0.5336211913720561 1105 20-21 0.46260218291900657 1105 22-23 0.38770194239816647 1105 24-25 0.25424111890804113 1105 26-27 0.0412716330622942 1105 28-29 0.16764389330066365 1105 30-31 0.2003757709187184 1105 32-33 0.24940415314785014 1105 34-35 0.27332438879655285 1105 36-37 0.16734795395917956 1105 38-39 0.15534432439780232 1105 40-41 0.31961847232341256 1105 42-43 0.2534078171070817 1105 44-45 0.07779478296423292 1105 46-47 0.02035439976258857 1105 48-49 0.09389181368268495 1105 50-51 0.28036475098602054 1105 52-53 0.19358829102831976 1105 54-55 0.26587488316799934 1105 56-57 0.32124045521321776 1105 58-59 0.30743899945949593 1105 60-61 0.196914304182922 1105 62-63 0.2742018093848557 1105 64-65 0.2527788989711155 1105 66-67 0.2666511686792674 1105 68-69 0.1576249432500063 1105 70-71 -0.19709265359895056 1105 72-73 -0.11221133112666237 1105 74-75 -9.230807896578597E-4 1105 76-77 0.13518803934193357 1106 1 0.2645882637282213 1106 2 0.35812473601013295 1106 3 0.1093301330171812 1106 4 0.20206806772308283 1106 5 0.06560515616335749 1106 6 0.23194232040619056 1106 7 0.28752480526125623 1106 8 0.24557967554130755 1106 9 0.19731748222454115 1106 10-11 0.007728416586715525 1106 12-13 0.046752886648498304 1106 14-15 0.1188100837844317 1106 16-17 0.32396109139960316 1106 18-19 0.256834740819464 1106 20-21 0.0704739613918548 1106 22-23 0.15526236970417528 1106 24-25 0.31027433283144035 1106 26-27 0.31760130706935286 1106 28-29 0.3952743077387879 1106 30-31 0.32843920616600286 1106 32-33 0.2910486062433364 1106 34-35 0.37071283991295445 1106 36-37 0.42542598304329005 1106 38-39 0.37965612718731023 1106 40-41 0.3783820377837728 1106 42-43 0.41207264404258837 1106 44-45 0.10711286148713128 1106 46-47 0.29154842396036074 1106 48-49 0.1572637762564888 1106 50-51 0.10798739866471863 1106 52-53 0.17737482116622516 1106 54-55 0.2356186807873435 1106 56-57 0.16638373754163638 1106 58-59 0.06525466069837549 1106 60-61 0.021011346485874327 1106 62-63 0.0093810824928795 1106 64-65 0.16784686770463608 1106 66-67 0.0819841181493075 1106 68-69 -0.1461615437392325 1106 70-71 -0.11190041192040923 1106 72-73 -0.055361528747543076 1106 74-75 -0.18557299171164132 1106 76-77 -0.10136683712823213 1107 1 0.26077320285027383 1107 2 0.23283557195186688 1107 3 0.15020717112543736 1107 4 0.16149625341366658 1107 5 0.27027735171859035 1107 6 -0.018841383103669784 1107 7 -0.026749707344841056 1107 8 0.06497047249957433 1107 9 0.08185060929914556 1107 10-11 0.1449097150276799 1107 12-13 0.08617502464893789 1107 14-15 0.12366397031262721 1107 16-17 0.27771968587476437 1107 18-19 0.17289133451713923 1107 20-21 0.14989366706620189 1107 22-23 0.2979851363473358 1107 24-25 0.17061587116411658 1107 26-27 0.02294570480482605 1107 28-29 0.22435915451288935 1107 30-31 0.26958330326787205 1107 32-33 0.35790314722814287 1107 34-35 0.4269004006471704 1107 36-37 0.44980579312783675 1107 38-39 0.3561947094866511 1107 40-41 0.33006913275593774 1107 42-43 0.4140266678741753 1107 44-45 0.40569378376934395 1107 46-47 0.283315189442245 1107 48-49 0.4104632433215407 1107 50-51 0.2149560365644767 1107 52-53 0.3161985312348463 1107 54-55 0.38296073752726656 1107 56-57 0.3016553760752636 1107 58-59 0.3021063174365928 1107 60-61 0.43546788131557435 1107 62-63 0.3190031799387718 1107 64-65 0.47665172251393173 1107 66-67 0.6236792270207445 1107 68-69 0.45329598602540955 1107 70-71 0.5730201364318894 1107 72-73 0.31080919262406326 1107 74-75 0.39545458088134566 1107 76-77 0.43522156999866723 1108 1 0.04803998128728182 1108 2 0.09084649645646437 1108 3 0.0032829600167829653 1108 4 0.06062970688999769 1108 5 0.04362730430734274 1108 6 -0.05924706038896943 1108 7 -0.023321648306875886 1108 8 0.14419462515724746 1108 9 -0.03495682813275636 1108 10-11 -0.032556879346117285 1108 12-13 0.162362066896371 1108 14-15 0.12918068983726272 1108 16-17 0.09368754030953852 1108 18-19 0.06614671670036643 1108 20-21 -0.08023665589517748 1108 22-23 -0.037299676592638775 1108 24-25 0.009224760260075016 1108 26-27 -0.15899080306736835 1108 28-29 -0.1451112687717142 1108 30-31 -0.12049441155750884 1108 32-33 -0.13818657874544726 1108 34-35 -0.1272814330031764 1108 36-37 0.09932856346588892 1108 38-39 0.08846050773870928 1108 40-41 0.0597634928549553 1108 42-43 0.011240084186745491 1108 44-45 -0.20979271094745044 1108 46-47 -0.24685672667192549 1108 48-49 -0.21762025988426004 1108 50-51 -0.19805147923720767 1108 52-53 -0.11097181320732474 1108 54-55 -0.1088100977051063 1108 56-57 -0.20002624319478812 1108 58-59 -0.1850739661485825 1108 60-61 -0.19705616360053213 1108 62-63 -0.22515162041296932 1108 64-65 -0.18470810349704792 1108 66-67 -0.25797790000424925 1108 68-69 -0.12683835445641023 1108 70-71 -0.27283086780192534 1108 72-73 -0.27067906349973114 1108 74-75 -0.31047822772105604 1108 76-77 -0.10971692817791734 1109 1 0.1666255962121781 1109 2 0.10303859028565654 1109 3 -0.023793823909485923 1109 4 0.13143568846515308 1109 5 0.04268652035956677 1109 6 0.028255483178874385 1109 7 -0.04536530534338823 1109 8 0.06652750938838636 1109 9 0.06127362673337444 1109 10-11 -0.15445764837761544 1109 12-13 -0.1368979901691958 1109 14-15 -0.11029487801706495 1109 16-17 0.04796954153044197 1109 18-19 -0.06685700609690315 1109 20-21 -0.1737087402941242 1109 22-23 -0.21077167583248269 1109 24-25 -0.3559828396965372 1109 26-27 -0.46624774779194667 1109 28-29 -0.2567424106476679 1109 30-31 -0.17412436299888867 1109 32-33 -0.1243189796314681 1109 34-35 -0.11568209132784091 1109 36-37 -0.042269841459358304 1109 38-39 -0.06597731222419867 1109 40-41 -0.16714460882321447 1109 42-43 -0.19221419299626064 1109 44-45 -0.22684918218742922 1109 46-47 -0.3846026908029181 1109 48-49 -0.2496115082180559 1109 50-51 -0.08077059135947096 1109 52-53 0.002796149760122546 1109 54-55 -0.1111073764684889 1109 56-57 -0.034747893954630626 1109 58-59 -0.07838507743897694 1109 60-61 -0.04971915587615072 1109 62-63 -0.1264857273494897 1109 64-65 -0.2963274799710831 1109 66-67 -0.12440080995343195 1109 68-69 -0.11236691009532862 1109 70-71 -0.1983546410850927 1109 72-73 -0.17138803541425673 1109 74-75 -0.09833884289246342 1109 76-77 0.10489475977404439 1110 1 -0.13986820589757798 1110 2 -0.1422868694074566 1110 3 -0.2749492892823753 1110 4 -0.10029148296428758 1110 5 -0.15031435111713165 1110 6 -0.35533669174080984 1110 7 -0.45633637318416476 1110 8 -0.16584871707415516 1110 9 -0.25353562392673723 1110 10-11 -0.18808277465501533 1110 12-13 -0.1613728279054314 1110 14-15 -0.17350978156930097 1110 16-17 -0.35522316321237923 1110 18-19 -0.26208122707911485 1110 20-21 -0.3161733934594011 1110 22-23 -0.5302309700664267 1110 24-25 -0.4649411649004236 1110 26-27 -0.39211651467410036 1110 28-29 -0.3714667898831152 1110 30-31 -0.2979574080078393 1110 32-33 -0.1253752657533056 1110 34-35 -0.06798868636866473 1110 36-37 -0.017516543071806723 1110 38-39 -0.0830560876476909 1110 40-41 0.03100740532852342 1110 42-43 0.11167254971866925 1110 44-45 -0.0859487429805128 1110 46-47 -0.1283085479067516 1110 48-49 -0.1778420419866933 1110 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-1.1340824549647763 2314 18-19 -0.8153885961047678 2314 20-21 -0.6654842884126637 2314 22-23 -0.4823045362088294 2314 24-25 -0.4760388680647907 2314 26-27 -0.5557569715227046 2314 28-29 -0.5220153832717216 2314 30-31 -0.6032494702315816 2314 32-33 -0.7916129687183684 2314 34-35 -0.8190791238753974 2314 36-37 -0.8951285333999621 2314 38-39 -0.7791417825462403 2314 40-41 -0.6472861254277689 2314 42-43 -0.5962041660228934 2314 44-45 -0.5850751427746843 2314 46-47 -0.6336229869064844 2314 48-49 -0.5221766862186037 2314 50-51 -0.6045159019580701 2314 52-53 -0.5955459735351383 2314 54-55 -0.6238536488192956 2314 56-57 -0.32915960945625855 2314 58-59 -0.4110804892163493 2314 60-61 -0.45640579729841235 2314 62-63 -0.28584315999819054 2314 64-65 -0.19003476354437154 2314 66-67 -0.15267997247274678 2314 68-69 -0.17945791141000456 2314 70-71 -0.06564610929646975 2314 72-73 -2.740605206490443E-4 2314 74-75 0.13407251204757387 2314 76-77 0.006187586966497349 2315 1 -1.2733458066281003 2315 2 -1.2219720026611824 2315 3 -1.0521309059057273 2315 4 -1.1940202638597555 2315 5 -1.075023519931598 2315 6 -1.0943823656100378 2315 7 -1.12654524571915 2315 8 -1.2873843633686803 2315 9 -1.1173580075267253 2315 10-11 -1.1459422859469583 2315 12-13 -1.2770058483892441 2315 14-15 -1.448824387157373 2315 16-17 -1.3782783000142587 2315 18-19 -1.1916521711570383 2315 20-21 -1.0517947428728789 2315 22-23 -1.1054158970952201 2315 24-25 -1.1408023115830872 2315 26-27 -0.9978302960828813 2315 28-29 -0.9003229709576033 2315 30-31 -1.1242853074493198 2315 32-33 -1.1824410361970408 2315 34-35 -1.1682774012721495 2315 36-37 -1.3846822920825588 2315 38-39 -1.229873739781354 2315 40-41 -1.2213362644666361 2315 42-43 -1.3380388254323918 2315 44-45 -1.3370363318418512 2315 46-47 -1.1967893635848874 2315 48-49 -1.1657315054236435 2315 50-51 -1.12164911493808 2315 52-53 -1.241781134709477 2315 54-55 -1.090248383398329 2315 56-57 -1.3327195154880052 2315 58-59 -1.108041911522296 2315 60-61 -1.3177934188880123 2315 62-63 -1.5940369493730984 2315 64-65 -1.6240911000708032 2315 66-67 -1.5991958611109105 2315 68-69 -1.567975248984581 2315 70-71 -1.5881957772564768 2315 72-73 -2.7907538406224965 2315 74-75 -3.3159297410072988 2315 76-77 -2.910996938544905 2316 1 -3.479068886169685 2316 2 -3.3249541029675527 2316 3 -2.8546332461984747 2316 4 -2.7185241927148525 2316 5 -3.448167242273591 2316 6 -3.0026368618088384 2316 7 -3.1347944230707867 2316 8 -3.046220032266902 2316 9 -2.81588681440293 2316 10-11 -2.8387169866731625 2316 12-13 -2.7206946816469255 2316 14-15 -2.832332060480873 2316 16-17 -4.122109501080864 2316 18-19 -3.8378429901176645 2316 20-21 -3.221796267609058 2316 22-23 -3.232675697957582 2316 24-25 -3.266694991431038 2316 26-27 -2.99824250673759 2316 28-29 -3.146891959951141 2316 30-31 -3.381489154039585 2316 32-33 -3.4989899199483148 2316 34-35 -3.354719837162314 2316 36-37 -3.364990324250943 2316 38-39 -3.4514624085093715 2316 40-41 -3.3015712511340602 2316 42-43 -3.4836257779457256 2316 44-45 -3.33162972370614 2316 46-47 -3.137970537702671 2316 48-49 -3.0884004320093688 2316 50-51 -3.524882087535378 2316 52-53 -4.3947455055253215 2316 54-55 -3.5961170220571255 2316 56-57 -3.574368216270937 2316 58-59 -3.615558151735616 2316 60-61 -3.4596596605196943 2316 62-63 -3.475176317352222 2316 64-65 -3.3856191694002042 2316 66-67 -3.4026798832940486 2316 68-69 -3.2813064075004093 2316 70-71 -3.2168319184683227 2316 72-73 -3.2907923136169366 2316 74-75 -3.286290819811338 2316 76-77 -3.335604762214487 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 50669.0 3 4807.0 4 6732.0 5 8133.0 6 9138.0 7 9744.0 8 11531.0 9 12743.0 10 16982.0 11 19440.0 12 14105.0 13 10968.0 14 10315.0 15 10455.0 16 10483.0 17 10907.0 18 11125.0 19 12052.0 20 12755.0 21 13959.0 22 14991.0 23 17301.0 24 19942.0 25 23113.0 26 27527.0 27 32424.0 28 38522.0 29 45435.0 30 54466.0 31 65194.0 32 79620.0 33 101064.0 34 135354.0 35 179608.0 36 252299.0 37 353451.0 38 338808.0 39 98339.0 40 1044.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 21.742052889375323 26.890451161325085 30.318676851995146 21.048819097304445 2 21.381950329517473 27.01227544658964 30.238973587326097 21.36680063656679 3 21.614158216182602 27.313188908290996 29.609899489195158 21.46275338633124 4 21.167460608623053 27.14293239381075 30.137707247286155 21.551899750280043 5 21.57257322069436 27.22023101151795 30.02730203923905 21.17989372854865 6 21.661817337695087 27.025833980480247 30.074711673908812 21.237637007915858 7 21.572580645161292 26.803002051090807 30.07332649636398 21.551090807383925 8 21.39614955921641 27.34214791737854 29.722999295622653 21.538703227782396 9 21.405356420301665 26.94269529043001 30.070801113574536 21.581147175693793 10-11 21.81642304886264 27.105885753311625 29.722786170309472 21.354905027516264 12-13 21.90424513052662 26.78687594558114 29.705914504430993 21.60296441946125 14-15 21.623729876948563 27.15209500676575 29.724736559733717 21.49943855655197 16-17 21.842701154193588 26.974812085798693 29.80816111166527 21.37432564834245 18-19 21.691089921241122 26.97970542921141 29.58578750719645 21.743417142351017 20-21 21.692210759217936 27.258764676169157 29.426323760213503 21.622700804399404 22-23 21.901638702305718 27.346163770467463 28.920579474486168 21.83161805274065 24-25 21.90666123107712 27.616990459514764 28.635184533836426 21.841163775571687 26-27 21.953484013626216 27.667678485097554 28.46211368574949 21.916723815526744 28-29 22.148092575514237 27.748112712177857 28.359973626564173 21.743821085743733 30-31 21.860438016085915 27.607412293070375 28.51723840576501 22.014911285078693 32-33 21.833285903969873 27.985725043473032 28.274566370926525 21.906422681630573 34-35 22.049175819309536 27.60422639074956 28.463390826940234 21.883206963000667 36-37 21.9321350251448 27.726661553101945 28.6777752683864 21.663428153366855 38-39 21.939641338571942 27.623936213728477 28.71990440170304 21.71651804599654 40-41 21.78048833923546 27.542674095720308 28.664187415016563 22.012650150027664 42-43 21.72767828008316 27.954708607974936 28.295326353474238 22.02228675846766 44-45 21.902045367460392 27.619779250600633 28.657076743932375 21.821098638006596 46-47 21.714246420154826 27.65554232061147 28.615800969233028 22.014410290000676 48-49 21.85236267871807 27.757212183399467 28.37743559715967 22.012989540722792 50-51 21.64154121034884 27.989969711438036 28.329527058279368 22.038962019933752 52-53 21.71199338639357 27.91099048128395 28.329469878692265 22.04754625363022 54-55 21.737490538543646 27.86187054653008 28.31268694170997 22.0879519732163 56-57 21.67647128751089 28.134258117540767 28.177706441307237 22.011564153641107 58-59 21.79903737457317 28.070693533161627 28.179309380353583 21.950959711911615 60-61 21.73055169467209 28.010496526836516 28.021638247896767 22.23731353059463 62-63 21.675280706105696 27.86773851860278 28.19107969050998 22.265901084781543 64-65 21.71471928930993 27.810877371696176 28.175663154165058 22.298740184828837 66-67 21.563872939694445 27.988359464473827 28.3324751033664 22.115292492465333 68-69 21.759283113242454 27.740869945139117 28.33820563352764 22.161641308090786 70-71 21.519050209469412 27.56357239839737 28.41655954389834 22.500817848234885 72-73 21.681910908072254 27.423447193596346 28.36919349278404 22.52544840554736 74-75 21.43942737134375 27.51378718060437 28.261201180412176 22.78558426763971 76-77 21.31477430548266 27.37617556760106 28.74696938100963 22.562080745906652 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 13188.0 1 7855.5 2 2322.0 3 2216.0 4 2311.0 5 2366.0 6 2421.0 7 2585.0 8 2799.0 9 2849.0 10 2918.5 11 3064.5 12 3246.5 13 3352.0 14 3469.5 15 3871.5 16 4366.0 17 4576.0 18 4991.5 19 6036.0 20 7372.5 21 9410.0 22 10740.0 23 12235.0 24 15149.0 25 18848.0 26 21128.0 27 23599.0 28 29764.5 29 35896.0 30 38333.0 31 43124.0 32 52779.0 33 61792.5 34 69037.0 35 72132.0 36 76029.5 37 83012.0 38 86847.0 39 87597.0 40 89529.5 41 93061.5 42 97525.5 43 100390.0 44 98661.0 45 96250.0 46 96365.0 47 98027.0 48 98892.0 49 97965.5 50 94901.5 51 88589.5 52 84415.0 53 82064.0 54 75800.5 55 66449.5 56 56334.0 57 51657.0 58 49096.0 59 42341.0 60 35078.5 61 32010.0 62 28213.0 63 20660.0 64 14507.0 65 12110.0 66 10371.5 67 7676.5 68 5811.0 69 4032.0 70 3162.0 71 2623.5 72 1883.0 73 1504.5 74 1328.0 75 1208.5 76 868.5 77 528.0 78 408.0 79 336.0 80 257.5 81 207.0 82 138.5 83 114.0 84 97.0 85 72.5 86 61.0 87 57.0 88 51.5 89 43.5 90 35.5 91 30.0 92 36.5 93 43.0 94 33.0 95 21.0 96 19.0 97 21.5 98 29.5 99 67.0 100 99.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011046144452808028 2 0.013376554674919425 3 0.014122285945995072 4 0.015940005919241964 5 0.013236730061592743 6 0.01076649522615466 7 0.01607983053256865 8 0.01808398332358445 9 0.017757725892488856 10-11 0.020973691999002585 12-13 0.017221731541403232 14-15 0.00848269320848549 16-17 0.007037838870776423 18-19 0.018177199732468906 20-21 0.024632436047717476 22-23 0.02160290275897266 24-25 0.019738574581283544 26-27 0.026193810896532117 28-29 0.009531377808435618 30-31 0.008855558844023314 32-33 0.011628747008335877 34-35 0.012257957768305955 36-37 0.012607519301622664 38-39 0.014844713114849608 40-41 0.012770648017170462 42-43 0.015147666443724088 44-45 0.014308718763763984 46-47 0.025634512443225385 48-49 0.023840096572199605 50-51 0.03919749993591372 52-53 0.04080548298917058 54-55 0.030691502625207114 56-57 0.02181263967896269 58-59 0.017245035643624347 60-61 0.02118342891899261 62-63 0.027825098052010094 64-65 0.02738232010980893 66-67 0.02920004008305582 68-69 0.04374179986903094 70-71 0.05585993302401022 72-73 0.08827593921357976 74-75 0.08794968178248418 76-77 0.09349605811110931 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 2145545.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.31427498765815 #Duplication Level Percentage of deduplicated Percentage of total 1 77.09926196512839 43.41789039649733 2 12.831064135643299 14.4514414823779 3 4.4183068103212815 7.464412340888262 4 1.929176194411204 4.345606348468656 5 1.0112458282965988 2.84737878274084 6 0.6377538832844528 2.154878853463651 7 0.3874111050823909 1.5271742853417605 8 0.27249152466057597 1.2276130121233526 9 0.20383436565708274 1.0330906067593038 >10 1.0580809899655734 11.285590926551373 >50 0.09877556953525944 3.8311251210915303 >100 0.05052501799199795 4.930271341368851 >500 0.0018238968191754796 0.6592673004582695 >1k 1.6580880174321789E-4 0.2673298323365138 >5k 0.0 0.0 >10k+ 8.290440087160894E-5 0.5569293695323565 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11929 0.5559892707913374 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4678 0.2180331803807424 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.398246133266839E-4 0.0 3 0.0 0.0 0.0 4.6608204442227964E-4 0.0 4 0.0 0.0 0.0 8.389476799601033E-4 0.0 5 0.0 0.0 0.0 0.0015846789510357508 0.0 6 0.0 0.0 0.0 0.0029829250843025896 0.0 7 0.0 0.0 0.0 0.0055463763286251275 0.0 8 0.0 0.0 0.0 0.00801661116406321 0.0 9 0.0 0.0 0.0 0.011465618292788079 0.0 10 0.0 4.660820444222796E-5 0.0 0.015613748488146369 0.0 11 0.0 4.660820444222796E-5 0.0 0.020041527910158025 0.0 12 0.0 4.660820444222796E-5 0.0 0.026520068327627714 0.0 13 0.0 1.398246133266839E-4 0.0 0.03472311230945983 0.0 14 0.0 1.398246133266839E-4 0.0 0.04572264855782563 0.0 15 0.0 1.398246133266839E-4 0.0 0.058073822735016045 0.0 16 0.0 1.398246133266839E-4 0.0 0.07093768716107096 0.0 17 0.0 2.3304102221113982E-4 0.0 0.08240330545385904 0.0 18 0.0 3.2625743109559573E-4 0.0 0.09363588272443599 0.0 19 0.0 3.2625743109559573E-4 0.0 0.10705904560379764 0.0 20 0.0 3.2625743109559573E-4 0.0 0.12080846591425488 0.0 21 0.0 3.2625743109559573E-4 0.0 0.1356764831313256 0.0 22 0.0 3.2625743109559573E-4 0.0 0.15427315670377456 0.0 23 0.0 3.2625743109559573E-4 0.0 0.173988427182837 0.0 24 0.0 3.2625743109559573E-4 0.0 0.19389013047966833 0.0 25 0.0 3.728656355378237E-4 0.0 0.21379183377649968 0.0 26 0.0 3.728656355378237E-4 0.0 0.2339731862999844 0.0 27 0.0 3.728656355378237E-4 0.0 0.25606547520560047 0.0 28 0.0 3.728656355378237E-4 0.0 0.2784840215423121 0.0 29 0.0 3.728656355378237E-4 0.0 0.3006229186523704 0.0 30 0.0 3.728656355378237E-4 0.0 0.32425327830457995 0.0 31 0.0 3.728656355378237E-4 0.0 0.3504004809966698 0.0 32 0.0 3.728656355378237E-4 0.0 0.37729341495983537 0.0 33 0.0 3.728656355378237E-4 0.0 0.40623710991845896 0.0 34 0.0 3.728656355378237E-4 0.0 0.43960858429909416 0.0 35 0.0 3.728656355378237E-4 0.0 0.47321309970194053 0.0 36 0.0 5.126902488645077E-4 0.0 0.5119911257978742 0.0 37 0.0 5.126902488645077E-4 0.0 0.5494641221694255 0.0 38 0.0 5.126902488645077E-4 0.0 0.5868439021320924 0.0 39 0.0 5.126902488645077E-4 0.0 0.6236177754370101 0.0 40 0.0 5.126902488645077E-4 0.0 0.6643999543239596 0.0 41 0.0 5.126902488645077E-4 0.0 0.7091438305884985 0.0 42 0.0 5.126902488645077E-4 0.0 0.7579892288439534 0.0 43 0.0 5.126902488645077E-4 0.0 0.8122877870191489 0.0 44 0.0 5.126902488645077E-4 0.0 0.8688701472120137 0.0 45 0.0 5.126902488645077E-4 0.0 0.930253152462428 0.0 46 0.0 5.126902488645077E-4 0.0 0.9998858098991166 0.0 47 0.0 6.059066577489635E-4 0.0 1.0634127925538732 0.0 48 0.0 6.059066577489635E-4 0.0 1.1334649238305419 0.0 49 0.0 6.059066577489635E-4 0.0 1.2069660622359355 0.0 50 0.0 6.059066577489635E-4 0.0 1.2752470817437993 0.0 51 0.0 6.059066577489635E-4 0.0 1.3503328991002286 0.0 52 0.0 6.525148621911915E-4 0.0 1.433015853780741 0.0 53 0.0 6.525148621911915E-4 0.0 1.5230162965586833 0.0 54 0.0 6.525148621911915E-4 0.0 1.6218256899762065 0.0 55 0.0 6.525148621911915E-4 0.0 1.7269271909934305 0.0 56 0.0 6.525148621911915E-4 0.0 1.8424223216012714 0.0 57 0.0 6.525148621911915E-4 0.0 1.9544684450803875 0.0 58 0.0 6.525148621911915E-4 0.0 2.079891123234423 0.0 59 0.0 6.525148621911915E-4 0.0 2.1963650261355507 0.0 60 0.0 6.525148621911915E-4 0.0 2.3183853053653034 0.0 61 0.0 6.525148621911915E-4 0.0 2.446511259376988 0.0 62 0.0 6.525148621911915E-4 0.0 2.582793649166063 0.0 63 0.0 6.525148621911915E-4 0.0 2.725274930145954 0.0 64 0.0 6.525148621911915E-4 0.0 2.871298434663454 0.0 65 0.0 6.525148621911915E-4 0.0 3.0389015378377056 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTAAG 350 0.0 21.295937 38 TGCGTAA 360 0.0 20.704866 37 TATACGG 315 0.0 20.282318 12 TTGCGTA 355 0.0 19.996185 36 CGGTATA 130 9.504378E-5 19.111738 10 TCGAACT 580 0.0 18.975384 24 TATAGCG 95 0.006645934 18.68413 43 CTCGAAC 575 0.0 18.523388 23 CGTAAGT 385 0.0 18.4389 39 ATACGTA 330 0.0 18.283964 1 TACGTAG 335 0.0 18.01065 2 AGCGACG 120 0.0012184008 17.753235 46 CGAACTC 590 0.0 17.447859 25 CGGTTAC 265 1.5661499E-9 17.410961 4 CGATCGT 185 4.1613566E-6 17.266573 1 CGCGGTA 145 2.1893128E-4 17.138657 19 ATTCGCG 190 5.3548283E-6 16.815325 56 ATATACG 370 1.8189894E-12 16.3077 11 GCCTCGA 680 0.0 16.184885 21 CTTGCGT 440 0.0 16.13291 35 >>END_MODULE