FastQCFastQC Report
Thu 26 May 2016
SRR936153_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936153_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2145545
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114710.534642713156797No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81870.38158136976852036No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60050.27988226767557894No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA12700.048.735443100-101
CGGTATC9900.043.5831100-101
GGTATCA88600.039.485481
TCGTATC5000.038.683758100-101
CGTACAT17550.038.48877100-101
CGAGTAC10350.037.950623100-101
TACGCAG7750.036.476100-101
CCGTACA6100.035.610523100-101
GCGTATC3750.034.12106100-101
TCGAGTA3900.032.80871100-101
GACGCAG8200.031.934057100-101
GCGTACA4700.031.656103100-101
ACGTATC4050.031.593573100-101
TCGGTAT3400.031.507133100-101
AGGTATC21800.031.394722100-101
GTATCAA176550.031.3744241
AGTATCA34200.030.887842100-101
GGTACAT20250.030.711891100-101
CCGTATC5250.030.60693100-101
GTCGTAC2250.030.41799598-99
Produced by FastQC (version 0.11.5)