FastQCFastQC Report
Thu 26 May 2016
SRR936152_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936152_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2153872
Sequences flagged as poor quality0
Sequence length77
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121530.5642396576955362No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47210.21918665547442No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTATA958.426232E-626.1564510
ATACGTA3050.025.6003861
TACGTAG3200.024.40152
CGGTTAC1751.6916601E-1024.3386864
AATTGCG2153.6379788E-1223.11553819
CGTAGGC3600.021.6907274
CGATTCG850.003494700420.8802315
TGCGATA2251.7462298E-1020.51188322
TTGCGAT2251.7462298E-1020.51188321
ACCGGTA2554.5474735E-1119.48911910
ATTGCGA2455.493348E-1018.8361320
TCGCCCG1351.2670574E-418.4085358
ACGTAGG4750.017.9333523
GCGGTAT1803.2005228E-617.7486089
ACGATCG1250.001602206117.04222559
CGGTAGG2953.5652192E-1016.8473112
GCGATAA2752.5538611E-916.78205923
CCGGTAG3004.5474735E-1016.56536511
GTTCGCA1754.990051E-516.22541236
CGATAAT2956.4774213E-915.64356624
Produced by FastQC (version 0.11.5)