##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936152_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2153872 Sequences flagged as poor quality 0 Sequence length 77 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.71038761820572 40.0 36.0 41.0 30.0 41.0 2 36.58234194046814 39.0 36.0 41.0 30.0 41.0 3 36.4481422294361 39.0 36.0 41.0 30.0 41.0 4 36.33735013036986 39.0 36.0 41.0 30.0 41.0 5 36.26367908585097 39.0 36.0 41.0 29.0 41.0 6 36.15398872356389 39.0 35.0 41.0 29.0 41.0 7 35.99181195539939 39.0 35.0 40.0 29.0 41.0 8 35.841042550346536 39.0 35.0 40.0 28.0 41.0 9 35.99003144105128 39.0 35.0 41.0 29.0 41.0 10-11 36.16931252182117 39.0 35.0 41.0 30.0 41.0 12-13 36.05821237288009 39.0 35.0 41.0 29.0 41.0 14-15 35.89906340766768 39.0 35.0 41.0 28.0 41.0 16-17 35.73483196773067 39.0 35.0 41.0 27.0 41.0 18-19 35.547772105306166 39.0 35.0 41.0 27.0 41.0 20-21 35.28841500330567 39.0 35.0 41.0 25.5 41.0 22-23 34.937676658594384 39.0 34.5 41.0 24.0 41.0 24-25 34.66011884643099 39.0 34.0 41.0 23.0 41.0 26-27 33.70277829880327 38.0 33.0 40.0 21.5 40.5 28-29 33.71452621139974 38.0 33.0 39.5 20.5 40.5 30-31 34.12487464436141 38.0 34.0 40.0 21.5 41.0 32-33 34.01441473773743 38.0 34.0 40.0 20.0 41.0 34-35 33.77786168351694 38.0 33.0 40.0 19.0 41.0 36-37 33.48281559906995 38.0 33.0 40.0 17.5 41.0 38-39 33.18716200405595 37.0 33.0 40.0 14.5 41.0 40-41 32.84741409888795 37.0 32.0 40.0 12.5 41.0 42-43 32.488873990654966 36.0 32.0 39.0 8.0 41.0 44-45 32.132465392558146 36.0 32.0 39.0 7.5 41.0 46-47 31.722093977729408 35.0 31.0 39.0 4.5 40.0 48-49 31.3099246380472 35.0 31.0 38.0 2.0 40.0 50-51 30.86114773765572 35.0 31.0 37.0 2.0 39.5 52-53 30.386881857417713 35.0 30.0 37.0 2.0 39.0 54-55 29.92802357800278 35.0 30.0 36.0 2.0 39.0 56-57 29.490051405097425 34.0 29.0 36.0 2.0 37.5 58-59 29.067314817222194 34.0 29.0 35.0 2.0 37.0 60-61 28.702371357258 34.0 29.0 35.0 2.0 36.5 62-63 28.337807678450716 34.0 29.0 35.0 2.0 36.0 64-65 27.96329261906 34.0 27.5 35.0 2.0 36.0 66-67 27.622053678213007 34.0 27.0 35.0 2.0 35.5 68-69 27.266230073096267 34.0 26.5 35.0 2.0 35.0 70-71 26.82953072420274 33.0 25.5 35.0 2.0 35.0 72-73 26.377871804824057 33.0 25.0 35.0 2.0 35.0 74-75 25.841436259907738 33.0 23.5 35.0 2.0 35.0 76-77 24.127134760097164 31.0 10.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.0962621801945573 1101 2 0.8757678283998516 1101 3 1.0411319371416639 1101 4 0.8405024173173388 1101 5 0.9197624677378826 1101 6 1.025687025065885 1101 7 0.915500860117568 1101 8 1.0331887420085195 1101 9 0.9602458156478733 1101 10-11 1.1145177036074045 1101 12-13 1.2520799741801625 1101 14-15 1.2163611775694037 1101 16-17 1.1369133009956087 1101 18-19 1.1722136296740615 1101 20-21 1.0618843637656212 1101 22-23 0.988825701232507 1101 24-25 0.9706342062016589 1101 26-27 0.9370843511198643 1101 28-29 1.1477576184210818 1101 30-31 1.048761549751788 1101 32-33 1.0863778650399851 1101 34-35 1.0383455704554407 1101 36-37 1.0699458665577737 1101 38-39 1.017160008056429 1101 40-41 0.9073245217801897 1101 42-43 0.9984784181463695 1101 44-45 0.9633526483670352 1101 46-47 0.9123730264754109 1101 48-49 0.9470908140972156 1101 50-51 0.9374993553833058 1101 52-53 1.045915370263831 1101 54-55 1.0933338025423147 1101 56-57 1.1059124920601882 1101 58-59 0.9771074572545011 1101 60-61 0.8996201070789382 1101 62-63 0.7813256316864319 1101 64-65 0.9452212438287937 1101 66-67 0.9490790397245554 1101 68-69 0.8866020759719113 1101 70-71 0.729751299458492 1101 72-73 0.8404611458138085 1101 74-75 0.8950251271724774 1101 76-77 1.0652451222285713 1102 1 0.808294534969086 1102 2 0.8567089083995967 1102 3 0.7840046126782809 1102 4 0.6601848234155625 1102 5 0.8434684263385606 1102 6 0.8988169468567051 1102 7 0.8034889073108502 1102 8 0.7896382895605569 1102 9 0.96266290935813 1102 10-11 1.0274589268132672 1102 12-13 1.138157176011049 1102 14-15 1.0157742776925502 1102 16-17 1.040756889505566 1102 18-19 1.117342635338062 1102 20-21 1.0156810179409845 1102 22-23 0.9698703849750316 1102 24-25 1.0612963089089007 1102 26-27 1.0173288216225913 1102 28-29 1.149309099795218 1102 30-31 1.223212597044629 1102 32-33 1.2464609736328853 1102 34-35 1.1963036375455474 1102 36-37 1.1509559012852506 1102 38-39 1.1767459412919692 1102 40-41 1.175183722699849 1102 42-43 1.1095407721509218 1102 44-45 1.1531262600891452 1102 46-47 1.0677186853437242 1102 48-49 0.7954713343375914 1102 50-51 0.8723717131200388 1102 52-53 0.9045410132813814 1102 54-55 1.0460540812645824 1102 56-57 1.008708763208226 1102 58-59 1.0702256430095147 1102 60-61 0.8635007771462604 1102 62-63 0.8539464606242682 1102 64-65 0.788567480425904 1102 66-67 0.9350212992130658 1102 68-69 1.005152589723867 1102 70-71 0.9995007475999778 1102 72-73 1.063173146045294 1102 74-75 0.9731646638810609 1102 76-77 0.9612145810712782 1103 1 0.7678299309289116 1103 2 0.7626932905583175 1103 3 0.8008352960660332 1103 4 0.7539742528281579 1103 5 0.48959169156484705 1103 6 0.6573365905629558 1103 7 0.8583939202149082 1103 8 0.6952273839541192 1103 9 0.7255516752364883 1103 10-11 0.8304774704103011 1103 12-13 0.8653256341189746 1103 14-15 0.8014736287076047 1103 16-17 0.9726839282004036 1103 18-19 0.900957873163506 1103 20-21 1.0308325111941201 1103 22-23 0.9391671726175801 1103 24-25 1.1048763634735437 1103 26-27 1.1104895483739625 1103 28-29 0.9781829801143189 1103 30-31 1.0042157077979539 1103 32-33 1.076267309125008 1103 34-35 1.187970316256326 1103 36-37 1.1618476329058183 1103 38-39 0.960206894039068 1103 40-41 0.9776292074344184 1103 42-43 0.973142043623767 1103 44-45 1.092667225246494 1103 46-47 1.0742751387476979 1103 48-49 1.0380696251335308 1103 50-51 1.0575913712310516 1103 52-53 1.0635240180754195 1103 54-55 1.1049855613462718 1103 56-57 1.206343130938098 1103 58-59 1.097082447667983 1103 60-61 0.8034266721322005 1103 62-63 0.8702752658531487 1103 64-65 0.9549019704757811 1103 66-67 1.018784246031565 1103 68-69 1.0075379261616888 1103 70-71 1.0112460454580976 1103 72-73 1.0455114189099568 1103 74-75 0.9706522620586426 1103 76-77 1.0211751101065012 1104 1 0.4336383147543188 1104 2 0.38665667927642744 1104 3 0.431829685103736 1104 4 0.44764635254052365 1104 5 0.42767342117775087 1104 6 0.5488568878813638 1104 7 0.37185298127654676 1104 8 0.3788620223386374 1104 9 0.43235995033508345 1104 10-11 0.43448619491304186 1104 12-13 0.49308375321107434 1104 14-15 0.4824378707664252 1104 16-17 0.5910454151877857 1104 18-19 0.4568615741581965 1104 20-21 0.4080815911346747 1104 22-23 0.5782295851007362 1104 24-25 0.5442381041568112 1104 26-27 0.4596187454759004 1104 28-29 0.4208946596799308 1104 30-31 0.4761559541794895 1104 32-33 0.606650686443281 1104 34-35 0.6923721046475961 1104 36-37 0.6430700187673821 1104 38-39 0.6757017822316627 1104 40-41 0.592293159225612 1104 42-43 0.7301170331629336 1104 44-45 0.6492636478049931 1104 46-47 0.524303638350684 1104 48-49 0.42812211052953586 1104 50-51 0.49291951203556295 1104 52-53 0.5508869975853585 1104 54-55 0.45732714144333286 1104 56-57 0.5226598949646792 1104 58-59 0.38712356617032384 1104 60-61 0.44394454121853144 1104 62-63 0.46896790834292545 1104 64-65 0.21281431689916275 1104 66-67 0.3053444592679 1104 68-69 0.22124623519622943 1104 70-71 0.33175949957703565 1104 72-73 0.3001784348203138 1104 74-75 0.14294059896678135 1104 76-77 0.3055574924097293 1105 1 0.09572309113850253 1105 2 0.19110942873670922 1105 3 -0.061321937961601236 1105 4 0.23502381551037388 1105 5 0.13109630519233662 1105 6 0.04163021964934899 1105 7 0.2637268928737839 1105 8 0.23536243650238475 1105 9 0.2653410814260653 1105 10-11 0.32095967212751475 1105 12-13 0.18172229587646171 1105 14-15 0.15188139122350464 1105 16-17 0.13273572506053455 1105 18-19 0.12439570190325355 1105 20-21 0.21161307084065584 1105 22-23 0.08617207177901776 1105 24-25 0.22045499551546044 1105 26-27 0.05723746698175347 1105 28-29 0.12874453021299814 1105 30-31 0.3040839614322479 1105 32-33 0.30899250524174704 1105 34-35 0.2582567518909613 1105 36-37 0.3454509711483311 1105 38-39 0.2852497241313401 1105 40-41 0.48998982178325434 1105 42-43 0.3519529199625211 1105 44-45 0.26299073020075525 1105 46-47 0.11382659998020728 1105 48-49 0.026543070476314767 1105 50-51 0.13556468132091837 1105 52-53 0.10110679787364418 1105 54-55 0.19577784291280054 1105 56-57 0.1957232718015156 1105 58-59 0.09372116206390757 1105 60-61 0.2383399599804754 1105 62-63 0.18084853787182098 1105 64-65 0.19115877069218712 1105 66-67 0.05431068046395282 1105 68-69 -0.045711650546756744 1105 70-71 0.09813853441244547 1105 72-73 -0.4018225956973609 1105 74-75 -0.45377641957601256 1105 76-77 -0.38829560012162645 1106 1 0.12449480143830982 1106 2 0.18720877298314065 1106 3 0.24809008204214678 1106 4 0.30517220905269227 1106 5 0.2969503841711614 1106 6 0.21141243205789095 1106 7 -0.048655534377310516 1106 8 0.15781879044491376 1106 9 0.17090993742941407 1106 10-11 0.005606837629080985 1106 12-13 0.3406672658412475 1106 14-15 0.1986853852094228 1106 16-17 0.3322519535639543 1106 18-19 0.40083638934304133 1106 20-21 0.3707939495720396 1106 22-23 0.33928350761205905 1106 24-25 0.3075748465629715 1106 26-27 0.291702716176097 1106 28-29 0.42243673608795973 1106 30-31 0.7004946488861208 1106 32-33 0.47469651383544686 1106 34-35 0.23169476342783213 1106 36-37 0.33907568057777837 1106 38-39 0.7259442094791595 1106 40-41 0.6589802309000135 1106 42-43 0.6117981478417036 1106 44-45 0.5822256671500554 1106 46-47 0.6764570308724451 1106 48-49 0.42458961906882564 1106 50-51 0.30298275674690345 1106 52-53 0.4048794358385841 1106 54-55 0.4571965787424226 1106 56-57 0.5229584398451159 1106 58-59 0.3829725527396235 1106 60-61 0.16533717604566434 1106 62-63 0.058337439943731084 1106 64-65 0.2552064155735181 1106 66-67 0.10786866376222193 1106 68-69 0.21316281686777572 1106 70-71 0.023579856422156098 1106 72-73 0.04881117572877258 1106 74-75 0.22335830229588893 1106 76-77 0.10677885028962208 1107 1 -0.3078116055816551 1107 2 -0.2916128322752982 1107 3 -0.137666003924366 1107 4 -0.11648800885897259 1107 5 -0.2145588542336725 1107 6 -0.379828909836867 1107 7 -0.22175181153563983 1107 8 -0.21298867202668958 1107 9 -0.30592137497984595 1107 10-11 -0.2619907971696307 1107 12-13 -0.2678402530919328 1107 14-15 -0.48625346574878847 1107 16-17 -0.18721690995011642 1107 18-19 -0.10986756464651393 1107 20-21 -0.10202408420417441 1107 22-23 -0.2795050556925034 1107 24-25 -0.28028763635055043 1107 26-27 -0.3536766897491006 1107 28-29 -0.3024341047301391 1107 30-31 -0.0649503328809331 1107 32-33 -0.034995063669335025 1107 34-35 -0.14727665499490428 1107 36-37 -0.008993183113005898 1107 38-39 0.042836383762541175 1107 40-41 -0.012530107577894967 1107 42-43 0.022992098235029346 1107 44-45 -0.16599709632186688 1107 46-47 -0.16369097534451882 1107 48-49 -0.04406602978431451 1107 50-51 -0.025876246578054207 1107 52-53 0.01502679261481532 1107 54-55 0.020225132928310074 1107 56-57 0.026907547548898236 1107 58-59 0.01853612591834164 1107 60-61 -0.02194584669006261 1107 62-63 -0.03191571381997349 1107 64-65 -0.1447064842632848 1107 66-67 0.020593328284117973 1107 68-69 -0.19518541236801568 1107 70-71 -0.1637879912252309 1107 72-73 -0.1911501052334259 1107 74-75 -0.2269430249489055 1107 76-77 -0.1489040874444747 1108 1 0.2154973523708108 1108 2 0.32081861196601835 1108 3 0.3481658059476658 1108 4 0.2450293585998935 1108 5 0.3471413285816851 1108 6 0.24859887809731873 1108 7 0.32806537310787576 1108 8 0.36516489287276244 1108 9 0.31519379869474307 1108 10-11 0.3547825117883221 1108 12-13 0.3907520686630903 1108 14-15 0.27860028196601405 1108 16-17 0.20309387615592556 1108 18-19 0.17920921780320498 1108 20-21 0.11049876771538436 1108 22-23 0.23283498087057097 1108 24-25 0.32140340569697656 1108 26-27 0.1515792517499861 1108 28-29 0.25606680934664894 1108 30-31 0.3550679486547068 1108 32-33 0.2938440588169513 1108 34-35 0.3730615534146011 1108 36-37 0.36554977849576886 1108 38-39 0.4409168225193909 1108 40-41 0.23724137322650307 1108 42-43 0.3545734510686671 1108 44-45 0.3461144210455913 1108 46-47 0.3804132294086777 1108 48-49 0.3167657800877137 1108 50-51 0.2195533695096934 1108 52-53 0.3200999187574105 1108 54-55 0.3504353705882721 1108 56-57 0.29724575595840363 1108 58-59 0.33709188460207073 1108 60-61 0.2934106432550898 1108 62-63 0.29943711981805166 1108 64-65 0.5234836437074684 1108 66-67 0.5263063081744228 1108 68-69 0.4190741854381983 1108 70-71 0.34169646946946486 1108 72-73 0.3954586699036824 1108 74-75 0.45495825475859064 1108 76-77 0.4957485633781964 1109 1 0.0034951932145119713 1109 2 -0.1441553576394412 1109 3 -0.18115594398741308 1109 4 0.05401404590416092 1109 5 0.0989194073739128 1109 6 -0.052958192715195196 1109 7 0.055292384967955854 1109 8 0.03965765240012331 1109 9 0.0989153388250017 1109 10-11 -0.08064585667374047 1109 12-13 -0.039693011193442373 1109 14-15 0.05548131109595289 1109 16-17 -0.03951415380067402 1109 18-19 -0.1071289413802532 1109 20-21 -0.010631258646014885 1109 22-23 0.03831210009436603 1109 24-25 0.05404549498624789 1109 26-27 -0.17284501567306165 1109 28-29 -0.055085945869194575 1109 30-31 0.13162158213113884 1109 32-33 0.027287981012641183 1109 34-35 0.04616304055047493 1109 36-37 0.020441022879332138 1109 38-39 0.13865149072734795 1109 40-41 0.17079724224446835 1109 42-43 0.11972106980105224 1109 44-45 0.17401124664450407 1109 46-47 -0.05634700069763099 1109 48-49 0.008778981155749932 1109 50-51 0.07946388843711105 1109 52-53 0.13670806583805017 1109 54-55 0.16534967482901308 1109 56-57 0.19676817842313454 1109 58-59 0.30766849985297995 1109 60-61 0.2059011587536581 1109 62-63 0.1060977859992498 1109 64-65 0.08429202877058728 1109 66-67 0.03900726502694951 1109 68-69 -0.024390547493517545 1109 70-71 -0.009468575283801073 1109 72-73 -0.1411793013720697 1109 74-75 -0.06459742923734169 1109 76-77 -0.01218064956519882 1110 1 -0.36098604754342034 1110 2 -0.19696961240355648 1110 3 -0.4489510636859677 1110 4 -0.11815978597255139 1110 5 -0.2959544092733495 1110 6 -0.29873079248037016 1110 7 -0.2205620608029264 1110 8 -0.5271812052929619 1110 9 -0.44342866986069396 1110 10-11 -0.4186247859943606 1110 12-13 -0.26095848013764567 1110 14-15 -0.17955329905471729 1110 16-17 -0.4070547943218159 1110 18-19 -0.46955113609121213 1110 20-21 -0.49583842827750857 1110 22-23 -0.4424920393680907 1110 24-25 -0.6424917704925264 1110 26-27 -0.6946341811483947 1110 28-29 -0.3324817592445797 1110 30-31 -0.2290658064113984 1110 32-33 -0.23350650494433722 1110 34-35 -0.25111948537642803 1110 36-37 -0.3492139318499028 1110 38-39 -0.32382327014574486 1110 40-41 -0.16178014546290598 1110 42-43 -0.20277479122777464 1110 44-45 -0.3192448095177767 1110 46-47 -0.3653783094968155 1110 48-49 -0.2919295566697784 1110 50-51 -0.3312270618313704 1110 52-53 -0.19075333242185977 1110 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-0.9206918037719163 2314 22-23 -0.9539582322445384 2314 24-25 -0.9252787840487713 2314 26-27 -0.9189216695384417 2314 28-29 -1.0746746565656835 2314 30-31 -1.1710510291658522 2314 32-33 -1.2725947196735774 2314 34-35 -1.3172810541021036 2314 36-37 -1.830373204675844 2314 38-39 -1.2336092818548288 2314 40-41 -1.2076493522295486 2314 42-43 -1.200687087858018 2314 44-45 -1.1251530987594016 2314 46-47 -1.0508932693707465 2314 48-49 -0.865609936119391 2314 50-51 -0.8630698866229132 2314 52-53 -0.6568575036542406 2314 54-55 -0.7750828766920357 2314 56-57 -0.7608612216746593 2314 58-59 -0.7806751898834676 2314 60-61 -0.8840877431379646 2314 62-63 -0.8453051357700758 2314 64-65 -0.9000747472289312 2314 66-67 -0.7226457487687732 2314 68-69 -0.6596948938863569 2314 70-71 -0.6365191156298202 2314 72-73 -0.6709340441651648 2314 74-75 -0.7278104964358398 2314 76-77 -0.844458014368815 2315 1 -1.1469437617446516 2315 2 -1.0035374291510166 2315 3 -0.7807361707205729 2315 4 -0.6818968620848764 2315 5 -0.8225968915046522 2315 6 -0.883940795754242 2315 7 -1.1913157914368924 2315 8 -1.27099569272022 2315 9 -1.0090186539137562 2315 10-11 -1.1817934222667859 2315 12-13 -1.0267464808557847 2315 14-15 -0.9288536401714254 2315 16-17 -0.8994113510496291 2315 18-19 -0.9092920246843903 2315 20-21 -0.8445175251103052 2315 22-23 -1.0659295558694808 2315 24-25 -1.3334305278983507 2315 26-27 -1.230313087885186 2315 28-29 -1.1442302199950376 2315 30-31 -1.1745351851143084 2315 32-33 -1.29020210844633 2315 34-35 -1.2381021338361293 2315 36-37 -1.2622479167900806 2315 38-39 -1.1557696947568346 2315 40-41 -1.15696728441252 2315 42-43 -1.2214987190335407 2315 44-45 -1.212179046863195 2315 46-47 -1.1151222079433047 2315 48-49 -0.9768478153904354 2315 50-51 -1.0899321384506173 2315 52-53 -1.1196880555681616 2315 54-55 -1.1953748371498172 2315 56-57 -1.4281869252970019 2315 58-59 -1.2239117935991146 2315 60-61 -1.3435912213885643 2315 62-63 -1.0926601299237824 2315 64-65 -0.8759065156447612 2315 66-67 -0.9883049628849179 2315 68-69 -1.1136579736440027 2315 70-71 -1.3318586141475812 2315 72-73 -1.1864355854612718 2315 74-75 -1.1138632430136859 2315 76-77 -1.3103191914229875 2316 1 -1.8155051857921976 2316 2 -1.5467316220070444 2316 3 -1.74492351597722 2316 4 -2.5562100586771592 2316 5 -1.9269720249893325 2316 6 -1.8842529851139673 2316 7 -1.6034120744323346 2316 8 -1.4127402089717336 2316 9 -1.5173146115361575 2316 10-11 -1.4987252125831176 2316 12-13 -2.2286066970152802 2316 14-15 -3.1493123264975864 2316 16-17 -2.3881920459346304 2316 18-19 -2.036663437736749 2316 20-21 -1.6328072600393853 2316 22-23 -2.0852201688311354 2316 24-25 -1.9756957992051483 2316 26-27 -1.9155365643140954 2316 28-29 -2.474402899734635 2316 30-31 -2.6211191637884035 2316 32-33 -2.665037378461122 2316 34-35 -2.434621380679296 2316 36-37 -2.515906517686041 2316 38-39 -2.7089963985487273 2316 40-41 -2.383151112265402 2316 42-43 -2.6561127142926892 2316 44-45 -2.324360496381594 2316 46-47 -2.393677382930189 2316 48-49 -2.378788188277422 2316 50-51 -2.3760383935757723 2316 52-53 -2.4614531383484426 2316 54-55 -2.398735882562292 2316 56-57 -2.243568315752551 2316 58-59 -2.483628819257177 2316 60-61 -2.3194883043692336 2316 62-63 -2.3226250349881568 2316 64-65 -2.321226443820116 2316 66-67 -2.1262528451916403 2316 68-69 -2.230484030908599 2316 70-71 -2.106648532329711 2316 72-73 -2.108281457785317 2316 74-75 -2.2432868264940176 2316 76-77 -2.623341231886318 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 50336.0 3 4971.0 4 6892.0 5 8631.0 6 9353.0 7 9853.0 8 11868.0 9 12884.0 10 17008.0 11 19735.0 12 14492.0 13 11021.0 14 10353.0 15 10521.0 16 10813.0 17 10866.0 18 11527.0 19 12094.0 20 12814.0 21 13916.0 22 15490.0 23 17700.0 24 20280.0 25 23427.0 26 27712.0 27 33233.0 28 38625.0 29 45587.0 30 54148.0 31 65325.0 32 79816.0 33 101026.0 34 135283.0 35 178269.0 36 252397.0 37 356234.0 38 342867.0 39 95597.0 40 908.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 21.721632674286013 26.84054719623218 30.35389281164222 21.083927317839592 2 21.36288417392855 26.982484194729846 30.26621015251807 21.38842147882354 3 21.665828547230237 27.283736164644733 29.61863011815859 21.43180516996644 4 21.15312816931233 27.186755850109407 30.16628093155957 21.4938350490187 5 21.57139348034133 27.207685438983198 30.028663912491716 21.192257168183755 6 21.610235207695442 27.080088745004844 30.119391563652435 21.190284483647275 7 21.550721360240836 26.78549951915509 30.133768222071872 21.530010898532204 8 21.35966918431458 27.35558739055429 29.725396047764878 21.559347377366244 9 21.407154416425392 26.938163311268426 30.03995830034432 21.61472397196186 10-11 21.81161257606491 27.09036864082503 29.76812741011524 21.329891372994823 12-13 21.870639234525306 26.798556724489014 29.715849329095146 21.614954711890537 14-15 21.64337594644427 27.116165107890115 29.7595925930913 21.480866352574317 16-17 21.766781728820273 27.016290321569663 29.810143964274626 21.406783985335437 18-19 21.684153731392836 27.04946672829877 29.536210679642533 21.730168860665863 20-21 21.698199064103957 27.261895807040858 29.42904583736136 21.610859291493824 22-23 21.86295248171476 27.372216932584166 28.9106077480699 21.854222837631173 24-25 21.891103014947618 27.56967345671688 28.66328243833473 21.87594109000077 26-27 21.96131775753878 27.640859621897867 28.473791301812128 21.924031318751226 28-29 22.131196065589904 27.694001366475753 28.443357537242036 21.73144503069231 30-31 21.81593159577133 27.64791629763575 28.542714221141818 21.9934378854511 32-33 21.8717990864018 27.968590534721464 28.274972813999465 21.884637564877266 34-35 22.02500406287001 27.591019896454856 28.493441366981635 21.890534673693494 36-37 21.939544125851185 27.779994906408152 28.599310995356102 21.68114997238456 38-39 21.901133074625232 27.630680174467763 28.728115490863566 21.740071260043436 40-41 21.768108108861085 27.593753177808207 28.646916545126384 21.99122216820432 42-43 21.75566821100337 27.959861949996924 28.265471131041863 22.01899870795784 44-45 21.89305630304429 27.643490362464572 28.63396775585127 21.829485578639865 46-47 21.773134907754766 27.679862069798144 28.591378990904797 21.955624031542293 48-49 21.846230711742308 27.788556839111266 28.368559852656666 21.996652596489756 50-51 21.622018246370114 27.96185398724287 28.32854611864088 22.087581647746138 52-53 21.6894347342487 27.930160897440366 28.348554616658188 22.031849751652747 54-55 21.75358615145775 27.81144104101836 28.326242840816157 22.108729966707735 56-57 21.672255897946922 28.132299008240363 28.125565927080267 22.069879166732452 58-59 21.758411170470875 28.073400246571335 28.211986338740726 21.956202244217067 60-61 21.742208414888704 28.01580722981084 28.073157196956505 22.168827158343955 62-63 21.659808615439914 27.92409912467257 28.170928596622357 22.245163663265156 64-65 21.69306776208923 27.83178681982274 28.159563787529386 22.315581630558647 66-67 21.533532765623097 27.962693283230696 28.35255678572855 22.151217165417663 68-69 21.762838672524452 27.71715909829747 28.33359650019041 22.186405728987673 70-71 21.483780262701607 27.63683527042341 28.38938890090239 22.48999556597259 72-73 21.67658920282184 27.45170734290905 28.413483684786012 22.458219769483094 74-75 21.43093615752885 27.503752617423068 28.300903578699494 22.764407646348594 76-77 21.266879392998153 27.35065113075235 28.733759482951886 22.64870999329761 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 13483.0 1 7913.5 2 2233.0 3 2173.5 4 2225.0 5 2326.0 6 2415.0 7 2532.5 8 2692.0 9 2722.0 10 2768.0 11 2967.5 12 3235.0 13 3349.0 14 3590.5 15 3922.5 16 4325.0 17 4637.0 18 5020.0 19 6030.5 20 7398.0 21 9430.0 22 10722.0 23 12206.0 24 15147.0 25 18983.5 26 21363.0 27 23710.0 28 29630.0 29 35859.5 30 38516.0 31 43286.0 32 53045.5 33 62189.5 34 69324.5 35 72305.0 36 76638.5 37 83605.0 38 87324.5 39 88411.0 40 90275.5 41 93290.0 42 97785.0 43 101130.0 44 99450.0 45 97295.0 46 97204.5 47 98284.5 48 98980.0 49 98455.0 50 95551.5 51 88702.0 52 84231.0 53 82140.0 54 75855.0 55 66485.5 56 56796.0 57 52282.0 58 49673.5 59 42417.0 60 34774.0 61 31779.0 62 28143.0 63 20619.5 64 14440.5 65 12149.0 66 10389.0 67 7564.5 68 5661.5 69 3996.0 70 3169.0 71 2614.0 72 1832.0 73 1451.5 74 1298.0 75 1185.0 76 871.5 77 539.5 78 408.0 79 347.5 80 247.0 81 174.5 82 131.5 83 121.0 84 101.5 85 76.0 86 65.5 87 61.0 88 54.0 89 39.0 90 30.0 91 29.0 92 25.0 93 23.5 94 22.0 95 20.5 96 23.0 97 20.0 98 19.0 99 40.5 100 60.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0025999688003743955 2 0.007428482286783987 3 0.01118915144446838 4 0.018942629831299167 5 0.013603408187673176 6 0.013278412087626376 7 0.01722479330248037 8 0.01889620181700677 9 0.01750336138823477 10-11 0.020799750402995164 12-13 0.020289042245778766 14-15 0.007103486186737187 16-17 0.005037439550725391 18-19 0.010794513322982981 20-21 0.009935595058573584 22-23 0.013487338151942177 24-25 0.020962248453018563 26-27 0.012721275916117578 28-29 0.0072659842367605865 30-31 0.006755276079544188 32-33 0.008821322715555984 34-35 0.010307019172912782 36-37 0.008612396651240185 38-39 0.016296233016632373 40-41 0.012303423787485978 42-43 0.014137330352035776 44-45 0.01694622521672597 46-47 0.014810536559275575 48-49 0.01218735375175498 50-51 0.02632468410379075 52-53 0.02957464510425875 54-55 0.02409613941775556 56-57 0.015135532659322373 58-59 0.015646240816538773 60-61 0.019824762102854766 62-63 0.026162186053767352 64-65 0.019778334088562367 66-67 0.019778334088562367 68-69 0.02841394474694875 70-71 0.05578325917231851 72-73 0.06725097870254128 74-75 0.07927583440427287 76-77 0.07681514964677567 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 2153872.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.16689013233398 #Duplication Level Percentage of deduplicated Percentage of total 1 77.14580953088404 43.33040208091128 2 12.719899824079459 14.288744318267307 3 4.4313449998496885 7.466846032350751 4 1.9792190128407192 4.446663073682049 5 1.0161759265132024 2.8537720809794864 6 0.6124605071992848 2.0640001210953467 7 0.3871174048150569 1.5220226521193092 8 0.27145218807494137 1.2197300179029515 9 0.19704017542273347 0.9960420496162036 >10 1.0861973707137462 11.463745562699296 >50 0.09874547345052143 3.8137237377494615 >100 0.05271270851214717 5.117836618746946 >500 0.001576030692803247 0.5781336300033886 >1k 1.6589796766349033E-4 0.27213139921425117 >5k 0.0 0.0 >10k+ 8.294898383174516E-5 0.566206624662009 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12153 0.5642396576955362 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4721 0.21918665547442 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 4.642801429239992E-5 0.0 2 0.0 0.0 0.0 2.321400714619996E-4 0.0 3 0.0 0.0 0.0 3.2499610004679943E-4 0.0 4 0.0 0.0 0.0 8.821322715555985E-4 0.0 5 0.0 0.0 0.0 0.0014856964573567975 0.0 6 0.0 0.0 0.0 0.002367828728912396 0.0 7 0.0 0.0 0.0 0.004782085472117192 0.0 8 0.0 0.0 0.0 0.008264186544047186 0.0 9 0.0 0.0 0.0 0.01165343158739238 0.0 10 0.0 0.0 0.0 0.014439112444936375 0.0 11 0.0 0.0 0.0 0.018478349688375167 0.0 12 0.0 0.0 0.0 0.02428185147492516 0.0 13 0.0 0.0 0.0 0.033288886247650744 0.0 14 0.0 0.0 0.0 0.043967329534902726 0.0 15 0.0 9.285602858479984E-5 0.0 0.0582207299226695 0.0 16 0.0 9.285602858479984E-5 0.0 0.07038486966727828 0.0 17 0.0 1.857120571695997E-4 0.0 0.08357042572631986 0.0 18 0.0 2.785680857543995E-4 0.0 0.09522385731371223 0.0 19 0.0 2.785680857543995E-4 0.0 0.10734156904402861 0.0 20 0.0 2.785680857543995E-4 0.0 0.12219853361759658 0.0 21 0.0 2.785680857543995E-4 0.0 0.13821619854847456 0.0 22 0.0 3.2499610004679943E-4 0.0 0.15428029149364494 0.0 23 0.0 3.2499610004679943E-4 0.0 0.1719693649390493 0.0 24 0.0 3.2499610004679943E-4 0.0 0.19193341108478126 0.0 25 0.0 3.2499610004679943E-4 0.0 0.21282601751636124 0.0 26 0.0 3.2499610004679943E-4 0.0 0.2326972076335084 0.0 27 0.0 3.2499610004679943E-4 0.0 0.25456480236522877 0.0 28 0.0 3.2499610004679943E-4 0.0 0.2781038056114755 0.0 29 0.0 3.2499610004679943E-4 0.0 0.29932140814310226 0.0 30 0.0 3.2499610004679943E-4 0.0 0.32202470713208586 0.0 31 0.0 3.2499610004679943E-4 0.0 0.347002978821397 0.0 32 0.0 3.2499610004679943E-4 0.0 0.37174911043924613 0.0 33 0.0 3.2499610004679943E-4 0.0 0.4034594442009553 0.0 34 0.0 3.2499610004679943E-4 0.0 0.43530906200554165 0.0 35 0.0 3.2499610004679943E-4 0.0 0.46999078868196437 0.0 36 0.0 3.2499610004679943E-4 0.0 0.5065296359300832 0.0 37 0.0 3.714241143391994E-4 0.0 0.5440434714783423 0.0 38 0.0 3.714241143391994E-4 0.0 0.581046598869385 0.0 39 0.0 3.714241143391994E-4 0.0 0.6176783021460885 0.0 40 0.0 3.714241143391994E-4 0.0 0.6583028146519385 0.0 41 0.0 3.714241143391994E-4 0.0 0.7022237161725488 0.0 42 0.0 4.642801429239992E-4 0.0 0.7508338471366915 0.0 43 0.0 4.642801429239992E-4 0.0 0.8023689430012554 0.0 44 0.0 4.642801429239992E-4 0.0 0.8589646924236909 0.0 45 0.0 4.642801429239992E-4 0.0 0.921085375546922 0.0 46 0.0 4.642801429239992E-4 0.0 0.990866681028399 0.0 47 0.0 5.107081572163991E-4 0.0 1.061437262752847 0.0 48 0.0 5.107081572163991E-4 0.0 1.132843548734558 0.0 49 0.0 5.107081572163991E-4 0.0 1.2051319669878247 0.0 50 0.0 5.107081572163991E-4 0.0 1.278766797655571 0.0 51 0.0 5.107081572163991E-4 0.0 1.3565337215953408 0.0 52 0.0 5.57136171508799E-4 0.0 1.4374577505069939 0.0 53 0.0 5.57136171508799E-4 0.0 1.5239995691480275 0.0 54 0.0 5.57136171508799E-4 0.0 1.6222412473907455 0.0 55 0.0 6.03564185801199E-4 0.0 1.7226650423052066 0.0 56 4.642801429239992E-5 6.03564185801199E-4 0.0 1.8369243854788029 0.0 57 4.642801429239992E-5 6.03564185801199E-4 0.0 1.9490480399949486 0.0 58 4.642801429239992E-5 6.03564185801199E-4 0.0 2.0740786824843815 0.0 59 4.642801429239992E-5 6.03564185801199E-4 0.0 2.196602212202025 0.0 60 4.642801429239992E-5 6.03564185801199E-4 0.0 2.3166186291478787 0.0 61 4.642801429239992E-5 6.03564185801199E-4 0.0 2.4443885244805634 0.0 62 4.642801429239992E-5 6.03564185801199E-4 0.0 2.578054777628383 0.0 63 4.642801429239992E-5 6.03564185801199E-4 0.0 2.71863880490577 0.0 64 4.642801429239992E-5 6.03564185801199E-4 0.0 2.871108403842011 0.0 65 4.642801429239992E-5 6.03564185801199E-4 0.0 3.0348600102513057 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTATA 95 8.426232E-6 26.15645 10 ATACGTA 305 0.0 25.600386 1 TACGTAG 320 0.0 24.4015 2 CGGTTAC 175 1.6916601E-10 24.338686 4 AATTGCG 215 3.6379788E-12 23.115538 19 CGTAGGC 360 0.0 21.690727 4 CGATTCG 85 0.0034947004 20.88023 15 TGCGATA 225 1.7462298E-10 20.511883 22 TTGCGAT 225 1.7462298E-10 20.511883 21 ACCGGTA 255 4.5474735E-11 19.489119 10 ATTGCGA 245 5.493348E-10 18.83613 20 TCGCCCG 135 1.2670574E-4 18.40853 58 ACGTAGG 475 0.0 17.933352 3 GCGGTAT 180 3.2005228E-6 17.748608 9 ACGATCG 125 0.0016022061 17.042225 59 CGGTAGG 295 3.5652192E-10 16.84731 12 GCGATAA 275 2.5538611E-9 16.782059 23 CCGGTAG 300 4.5474735E-10 16.565365 11 GTTCGCA 175 4.990051E-5 16.225412 36 CGATAAT 295 6.4774213E-9 15.643566 24 >>END_MODULE