FastQCFastQC Report
Thu 26 May 2016
SRR936152_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936152_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2153872
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117280.5445077516212662No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80560.37402408313957375No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59420.2758752609254403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA13400.050.635036100-101
GGTATCA89100.040.2777751
TACGCAG10400.038.34357100-101
CGGTATC9700.037.735878100-101
TCGTATC5400.037.474533100-101
ACGTATC4800.037.198986100-101
CGTACAT17950.037.136814100-101
GCGTATC3150.036.844707100-101
CGAGTAC10650.036.6052100-101
CCGTACA7000.036.56129100-101
TATTACG1156.660139E-736.218062
CCGTATC4950.035.470547100-101
TCGAGTA4000.034.22307100-101
GTATCAA181950.033.3309751
AGTATCA32750.033.16673100-101
AGGTATC20900.032.891735100-101
GGTACAT20100.032.276134100-101
GCGTACA5550.030.027288100-101
GACGCAG7700.029.759188100-101
CCGAGTA5700.028.715006100-101
Produced by FastQC (version 0.11.5)