FastQCFastQC Report
Thu 26 May 2016
SRR936151_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936151_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188236
Sequences flagged as poor quality0
Sequence length77
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102885.465479504451858No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA92094.89226290401411No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2560.1359994900019125No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTGA400.002253923335.4938960
TTGATTT1500.005386477314.19755637
TGGGATT2200.005080171411.2935134
CTGGGAT2200.005080171411.2935133
TACATGG3852.7246697E-611.0630312
ACATGGG4004.307358E-610.6481673
ATGGGAA2700.001926833910.5195035
CATGGGA2700.001931103310.5167074
GTACATG3903.7986414E-510.0110981
TTTTTGG11608.649913E-76.121262619
TTTTGGG7750.0088304545.49728820
Produced by FastQC (version 0.11.5)