##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936151_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 188236 Sequences flagged as poor quality 0 Sequence length 77 %GC 31 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.92610340211224 40.0 36.0 41.0 31.0 41.0 2 36.704121422044665 40.0 35.0 41.0 31.0 41.0 3 36.56037102360866 39.0 35.0 41.0 31.0 41.0 4 36.39006353726174 39.0 35.0 41.0 31.0 41.0 5 36.24365158630655 39.0 35.0 41.0 31.0 41.0 6 35.950312373828595 38.0 35.0 40.0 30.0 41.0 7 35.483839435602114 38.0 35.0 40.0 30.0 41.0 8 35.028703329862516 37.0 35.0 40.0 29.0 41.0 9 34.76175651841306 37.0 35.0 40.0 29.0 41.0 10-11 34.65436207739221 37.0 35.0 40.0 28.0 41.0 12-13 34.3428329331265 37.0 35.0 40.0 24.5 41.0 14-15 33.96461888267919 37.0 34.0 40.0 22.0 41.0 16-17 33.42192513653074 36.5 33.5 40.0 16.5 41.0 18-19 32.77203882360441 36.0 33.0 40.0 2.0 41.0 20-21 31.81058086657175 36.0 33.0 40.0 2.0 41.0 22-23 30.543150619435178 35.0 30.5 40.0 2.0 41.0 24-25 29.54579889075416 35.0 27.0 40.0 2.0 41.0 26-27 28.39453664548758 35.0 23.0 39.0 2.0 40.5 28-29 28.289933912747827 35.0 22.0 39.5 2.0 40.5 30-31 28.460004993731275 35.0 21.0 40.0 2.0 41.0 32-33 28.211269363989885 35.0 18.5 40.0 2.0 41.0 34-35 27.892345247455324 35.0 10.5 40.0 2.0 41.0 36-37 27.570993327525024 35.0 4.0 40.0 2.0 41.0 38-39 27.235316305063854 35.0 2.0 39.0 2.0 41.0 40-41 26.824167534371746 35.0 2.0 39.0 2.0 41.0 42-43 26.407408784717056 35.0 2.0 39.0 2.0 41.0 44-45 26.003870141736968 35.0 2.0 38.0 2.0 40.0 46-47 25.594216834186874 34.5 2.0 37.5 2.0 40.0 48-49 25.114093478399457 34.0 2.0 37.0 2.0 40.0 50-51 24.650205061731018 34.0 2.0 36.0 2.0 39.0 52-53 24.169816081939693 33.5 2.0 36.0 2.0 39.0 54-55 23.67884995431267 33.0 2.0 35.0 2.0 38.5 56-57 23.173173569349114 33.0 2.0 35.0 2.0 37.0 58-59 22.672172166854374 32.5 2.0 35.0 2.0 37.0 60-61 22.22196604262734 32.0 2.0 35.0 2.0 36.5 62-63 21.79166578125332 31.5 2.0 35.0 2.0 36.0 64-65 21.334234684119934 31.0 2.0 35.0 2.0 36.0 66-67 20.887184173059353 31.0 2.0 35.0 2.0 35.0 68-69 20.452118617055184 30.0 2.0 35.0 2.0 35.0 70-71 19.99607142098217 29.5 2.0 35.0 2.0 35.0 72-73 19.52140132599503 29.0 2.0 34.5 2.0 35.0 74-75 19.021345544954208 28.0 2.0 34.0 2.0 35.0 76-77 17.973400412248452 23.5 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4645622263542748 1101 2 0.8636984250141069 1101 3 0.5136730203713498 1101 4 1.1469260506577257 1101 5 0.8894415285457953 1101 6 0.5034370729173503 1101 7 1.005828173202623 1101 8 1.5164782513977357 1101 9 1.4567020543530518 1101 10-11 1.5043670333844972 1101 12-13 1.624930432190368 1101 14-15 1.6763113054024714 1101 16-17 1.7507700916660909 1101 18-19 2.161609355415443 1101 20-21 2.323566408259129 1101 22-23 2.357916684603026 1101 24-25 2.3646224839168077 1101 26-27 2.3592063905416154 1101 28-29 2.1444916322478527 1101 30-31 2.076188255258902 1101 32-33 2.464751327178167 1101 34-35 2.4603175368177226 1101 36-37 2.299481748020284 1101 38-39 2.6079064546960105 1101 40-41 2.748261807220185 1101 42-43 2.6038545407503193 1101 44-45 1.846835295170603 1101 46-47 1.4720335511029923 1101 48-49 1.669056929518682 1101 50-51 1.1713205823702815 1101 52-53 1.0005080915545186 1101 54-55 1.4759049561284563 1101 56-57 1.6408789177867114 1101 58-59 1.710541498949695 1101 60-61 2.0180117181611266 1101 62-63 1.861566113851456 1101 64-65 2.065819185624264 1101 66-67 2.277110781478587 1101 68-69 1.8725185680883776 1101 70-71 1.911038302804183 1101 72-73 2.1322091287082543 1101 74-75 2.262819241881509 1101 76-77 2.2011616846035764 1102 1 1.440716873882927 1102 2 1.8108373685646768 1102 3 1.9021439082055878 1102 4 1.8919196841801238 1102 5 1.8028960178771243 1102 6 1.6119372851332727 1102 7 1.426440126746627 1102 8 1.311477672627042 1102 9 1.069080030199025 1102 10-11 1.2690581627590447 1102 12-13 1.4442382224438717 1102 14-15 1.6198232859557677 1102 16-17 1.0742643232515334 1102 18-19 0.5805428739553946 1102 20-21 0.33664662587690586 1102 22-23 -0.20657766273655653 1102 24-25 -0.4540872432892762 1102 26-27 0.11498445127394774 1102 28-29 0.2026966714113385 1102 30-31 0.3812389941561527 1102 32-33 0.16786125502475358 1102 34-35 0.01761583523189003 1102 36-37 0.09826247109779729 1102 38-39 0.18007915987022116 1102 40-41 0.36978435998478076 1102 42-43 0.2237758279363362 1102 44-45 0.6245294727374535 1102 46-47 0.28688480703538843 1102 48-49 0.41455216430665587 1102 50-51 -0.028895492021046465 1102 52-53 0.32531420337301853 1102 54-55 0.3600736484925413 1102 56-57 0.7837581090511314 1102 58-59 0.7814023238908518 1102 60-61 0.5044684839905038 1102 62-63 0.8870706089704896 1102 64-65 0.46221923192591774 1102 66-67 0.7613489263255993 1102 68-69 0.15557602072564336 1102 70-71 0.6618003508807355 1102 72-73 0.563759848313147 1102 74-75 0.23085180737906086 1102 76-77 0.6045648562669612 1103 1 0.2501740546671982 1103 2 0.8236454624189449 1103 3 0.536844155756981 1103 4 0.5931359149410795 1103 5 0.770606705639473 1103 6 0.5739435277336327 1103 7 0.34898165438986695 1103 8 0.7197222103517262 1103 9 0.9330343946377297 1103 10-11 0.9981328945783616 1103 12-13 0.7280971540264076 1103 14-15 0.8393367936513911 1103 16-17 1.0062594439776902 1103 18-19 0.6695537446905178 1103 20-21 0.8638952177041226 1103 22-23 0.5799182293453882 1103 24-25 0.27193794237427227 1103 26-27 -0.29532301473258116 1103 28-29 -0.3147933727173893 1103 30-31 0.1473567425147806 1103 32-33 0.042650477569871725 1103 34-35 0.18265009777987729 1103 36-37 0.42874358185014216 1103 38-39 1.016049453702962 1103 40-41 1.1103935516756671 1103 42-43 1.0783662918261214 1103 44-45 1.3690133818968526 1103 46-47 0.9016374350266396 1103 48-49 1.2629000278304972 1103 50-51 1.2186559018009326 1103 52-53 0.9673513002050775 1103 54-55 0.9955452518362797 1103 56-57 1.1254866635662566 1103 58-59 1.711424208869147 1103 60-61 1.318464106994849 1103 62-63 0.9959035297181664 1103 64-65 0.9186824759254861 1103 66-67 1.1911017337019096 1103 68-69 0.8135425115949424 1103 70-71 1.277252580637132 1103 72-73 0.8545968590403739 1103 74-75 0.9345614905850326 1103 76-77 0.8518292339801619 1104 1 1.3461575263203827 1104 2 1.347334925597849 1104 3 1.42170601099388 1104 4 1.3557160178930658 1104 5 1.2474425077195264 1104 6 1.3140422776023186 1104 7 0.9646275555699617 1104 8 1.1402495766717138 1104 9 1.364697384447581 1104 10-11 1.443225166175516 1104 12-13 1.8470522263379223 1104 14-15 1.4672388842071271 1104 16-17 1.2864534419466622 1104 18-19 1.9464735892119975 1104 20-21 1.816166114507002 1104 22-23 1.8248319107544972 1104 24-25 1.5388777469755972 1104 26-27 1.0980306288574369 1104 28-29 1.3149996727705826 1104 30-31 1.4740717335852729 1104 32-33 1.7714210066870741 1104 34-35 2.1021094247399894 1104 36-37 1.900694417021903 1104 38-39 1.2098723342576427 1104 40-41 1.4735094251917928 1104 42-43 1.5714853169337992 1104 44-45 1.6523939891034907 1104 46-47 1.4100066614859976 1104 48-49 1.2860116992245523 1104 50-51 1.3223957904446983 1104 52-53 1.080668525403805 1104 54-55 1.0042821639769102 1104 56-57 0.5455412911531887 1104 58-59 1.0015632290743497 1104 60-61 0.6203165424747645 1104 62-63 0.7556647091137592 1104 64-65 0.7528037071472546 1104 66-67 0.6565217708206568 1104 68-69 0.7184870085659121 1104 70-71 0.768813318809908 1104 72-73 1.1872123298531356 1104 74-75 0.8950265254276246 1104 76-77 1.3269666783895886 1105 1 -0.10088588685326982 1105 2 -0.18413962530035377 1105 3 -0.14363830038336545 1105 4 -0.3008333833045356 1105 5 0.11896196879737175 1105 6 0.19982072071609025 1105 7 0.23951528012086243 1105 8 -0.031045333507925932 1105 9 -0.4951454299236744 1105 10-11 0.21240555539707628 1105 12-13 0.4648478850205606 1105 14-15 -0.041200487050360834 1105 16-17 -0.2199649712269931 1105 18-19 0.011275235918589033 1105 20-21 -0.07778972381634475 1105 22-23 -0.4108529694850205 1105 24-25 -1.13217389973099 1105 26-27 -1.6584626346156206 1105 28-29 -1.1430280218401982 1105 30-31 -1.5148691346782037 1105 32-33 -1.3147691130734032 1105 34-35 -0.8797768028049191 1105 36-37 -0.5783287865709141 1105 38-39 -0.23230580945493173 1105 40-41 -0.23094416762257808 1105 42-43 -0.3812280064194944 1105 44-45 -0.4246269689803377 1105 46-47 -0.6227777696517229 1105 48-49 -0.33326225287125766 1105 50-51 -0.20310944907626194 1105 52-53 -0.21989285184170626 1105 54-55 0.024529169964935704 1105 56-57 0.10383096181186957 1105 58-59 0.10124511530189295 1105 60-61 -0.018250074291703555 1105 62-63 -0.39287571004791744 1105 64-65 0.09099607241671492 1105 66-67 0.07856518399431067 1105 68-69 -0.7085152369430681 1105 70-71 -0.9110332380763175 1105 72-73 -0.7445990473923736 1105 74-75 -0.5398537140933328 1105 76-77 -0.332034070113405 1106 1 -0.3634577259337277 1106 2 -0.3478608896681692 1106 3 -0.05269002452129712 1106 4 0.08012926037362433 1106 5 -0.18039147947849443 1106 6 0.33861382416436925 1106 7 -0.2536600072354602 1106 8 0.18281960902081096 1106 9 0.23581721375448694 1106 10-11 0.35662250942007034 1106 12-13 -0.028507000036910313 1106 14-15 -0.15143755601587827 1106 16-17 -0.48350663789366166 1106 18-19 -0.1993210284492335 1106 20-21 -0.005483689333587449 1106 22-23 0.5358065132735987 1106 24-25 -0.1630287847884624 1106 26-27 -0.7523203932363103 1106 28-29 -0.8212967000011169 1106 30-31 -0.8148332151379698 1106 32-33 -0.2700133659469657 1106 34-35 -0.5179951936095186 1106 36-37 -0.22491643024907404 1106 38-39 -0.6134121312940124 1106 40-41 -1.3241194549789022 1106 42-43 -0.9547553052700657 1106 44-45 -0.6560924862217199 1106 46-47 -0.21501914896206742 1106 48-49 -0.12442604597470464 1106 50-51 -0.44725456401878816 1106 52-53 -0.232033656439409 1106 54-55 -0.5488185311844909 1106 56-57 -0.31552967037204027 1106 58-59 -1.33147758584753 1106 60-61 -1.2953693271652647 1106 62-63 -1.3462521468295208 1106 64-65 -1.1185226057442037 1106 66-67 -1.211449183821781 1106 68-69 -0.7940396622304213 1106 70-71 -0.6865001920993059 1106 72-73 -1.1341105416452493 1106 74-75 -1.5872675071967812 1106 76-77 -1.2796992999984624 1107 1 -0.18360647508856687 1107 2 -0.12568374294741602 1107 3 0.061508758440162126 1107 4 0.0995342637542862 1107 5 -0.07552332532027606 1107 6 -0.2516498675192054 1107 7 0.10752998600321462 1107 8 0.6009399606097219 1107 9 0.16698692301750384 1107 10-11 0.5552364377500183 1107 12-13 0.6970170026676215 1107 14-15 0.9656009835378754 1107 16-17 1.5478659111259532 1107 18-19 1.9728561182715225 1107 20-21 1.2301146879483618 1107 22-23 1.1841837952208607 1107 24-25 1.3419069826219499 1107 26-27 1.570397659502028 1107 28-29 2.0295822722774517 1107 30-31 2.2805352770865035 1107 32-33 2.2561867692795374 1107 34-35 2.145223197195083 1107 36-37 1.8645020957820293 1107 38-39 1.697473602309774 1107 40-41 1.3253058323774205 1107 42-43 1.9671175818158027 1107 44-45 1.9540495016078978 1107 46-47 1.8139869362306271 1107 48-49 1.7156348059522735 1107 50-51 1.738986139159035 1107 52-53 1.48929832462888 1107 54-55 1.255779169964935 1107 56-57 1.2308530206354007 1107 58-59 0.9701789388313102 1107 60-61 1.3425955139435892 1107 62-63 1.1508742899520854 1107 64-65 1.1000034253578939 1107 66-67 1.3156975369354846 1107 68-69 1.2306391748216399 1107 70-71 1.3060623501589745 1107 72-73 1.463489187901743 1107 74-75 1.579263932965489 1107 76-77 0.8339585769454203 1108 1 -0.4273486528107142 1108 2 -0.32230451891737744 1108 3 -0.09762766208549323 1108 4 -0.3350089152194329 1108 5 -0.13728803120262967 1108 6 -0.23475374736901244 1108 7 -0.5297666347727557 1108 8 -0.741550652656862 1108 9 -0.5303847916257993 1108 10-11 -0.6084056148156876 1108 12-13 -0.4005111575326339 1108 14-15 -0.1468520827950428 1108 16-17 0.0315642840921555 1108 18-19 -0.1046157215282193 1108 20-21 -0.35352110679506765 1108 22-23 0.02498479647242391 1108 24-25 -0.5882244316458838 1108 26-27 -0.7816674218496615 1108 28-29 -0.9091849367338156 1108 30-31 -0.5169303048909697 1108 32-33 -0.26649783647766156 1108 34-35 -0.08243637727300168 1108 36-37 0.08649834108866372 1108 38-39 -0.28616219243365393 1108 40-41 -0.15075799740981566 1108 42-43 -0.3125444957811929 1108 44-45 -0.544573777490978 1108 46-47 -0.3972458547581077 1108 48-49 -0.15148033797763816 1108 50-51 -0.311952534182641 1108 52-53 -0.42587689439490006 1108 54-55 -0.5367740215244261 1108 56-57 -0.22828339989025892 1108 58-59 -0.20507137405980558 1108 60-61 -0.27057720195128 1108 62-63 -0.08316826323940418 1108 64-65 -0.03353850205136766 1108 66-67 -0.2534826883461143 1108 68-69 -0.412039173113282 1108 70-71 -0.28530185509759676 1108 72-73 -0.7475245793072673 1108 74-75 -0.920172863029503 1108 76-77 -0.7032441764963835 1109 1 0.2319945479293395 1109 2 -0.4262591905177473 1109 3 -0.025703517774672946 1109 4 -0.07610512243497425 1109 5 0.15619022966693308 1109 6 0.2563424598465289 1109 7 -0.26184341553130963 1109 8 0.4290090143181615 1109 9 0.16110457007632561 1109 10-11 0.037813164092732165 1109 12-13 0.24813592849882582 1109 14-15 0.09181581729746568 1109 16-17 0.3516382896425725 1109 18-19 0.14347632287510237 1109 20-21 -0.7792842890337361 1109 22-23 -0.6560975347024112 1109 24-25 -0.27225542147012405 1109 26-27 0.3256406262539464 1109 28-29 -0.02169650010106494 1109 30-31 0.21624499575657907 1109 32-33 0.38278523475268145 1109 34-35 0.316962327629863 1109 36-37 0.189470126472564 1109 38-39 0.26090071228420086 1109 40-41 0.6513927888991589 1109 42-43 0.03140786314572708 1109 44-45 0.32319911797618417 1109 46-47 0.16635266513088354 1109 48-49 0.5778790514765717 1109 50-51 0.561428594401999 1109 52-53 0.4537484525061224 1109 54-55 0.6796922134431966 1109 56-57 0.4061407444205649 1109 58-59 -0.24155379774158092 1109 60-61 -0.525722900378657 1109 62-63 -0.2512996230913913 1109 64-65 -0.1531615362789367 1109 66-67 -0.05268481600569075 1109 68-69 0.3500445456656287 1109 70-71 0.3035048054019427 1109 72-73 0.28311834391197266 1109 74-75 0.44655932938492526 1109 76-77 0.5012539733648573 1110 1 -0.05352763943059102 1110 2 0.08589903449345826 1110 3 0.27169675116302017 1110 4 0.3353135239119567 1110 5 0.5096191852922161 1110 6 0.2650269062831043 1110 7 0.19988899146106576 1110 8 0.2219830170075383 1110 9 0.1250220958495234 1110 10-11 0.5637264316857298 1110 12-13 0.9885270087319 1110 14-15 0.8563832758362366 1110 16-17 0.5310981731029045 1110 18-19 0.7859852874649818 1110 20-21 0.25606981226613 1110 22-23 0.7516792470098252 1110 24-25 0.6916340899597309 1110 26-27 0.8620592726008738 1110 28-29 0.4454706895000058 1110 30-31 0.32143885501252 1110 32-33 0.6229241858956698 1110 34-35 1.1843984549270417 1110 36-37 0.6307240484806336 1110 38-39 0.8827070771430066 1110 40-41 0.7737594406248434 1110 42-43 0.980337715229993 1110 44-45 0.9592647835969821 1110 46-47 1.0522222303482742 1110 48-49 0.756750633726682 1110 50-51 0.8372578189649786 1110 52-53 0.8987282821789115 1110 54-55 0.861449273057719 1110 56-57 0.9297008071726971 1110 58-59 0.7952850637555038 1110 60-61 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-0.6085450647067248 2314 8 -1.2553987817837893 2314 9 -0.4897574988891904 2314 10-11 -0.692228065292575 2314 12-13 -1.3050874598070266 2314 14-15 -1.3841961767055366 2314 16-17 -1.9439951436407839 2314 18-19 -1.3509014882193462 2314 20-21 -1.3977250686439326 2314 22-23 -1.5374693487953657 2314 24-25 -1.4460747617999559 2314 26-27 -1.368699703581136 2314 28-29 -1.543997849426404 2314 30-31 -1.7667010312299247 2314 32-33 -1.6895535958320238 2314 34-35 -1.7974492165980216 2314 36-37 -1.643738269329532 2314 38-39 -1.657952361179067 2314 40-41 -1.9548665814156827 2314 42-43 -2.0309047305574204 2314 44-45 -1.6647131758768907 2314 46-47 -1.2236398386172418 2314 48-49 -1.2817536321815979 2314 50-51 -0.9831741042486719 2314 52-53 -0.7399359552899831 2314 54-55 -1.0028415196902394 2314 56-57 -1.051880245084682 2314 58-59 -1.1260178157325882 2314 60-61 -0.8614612811882552 2314 62-63 -0.6486946755651566 2314 64-65 -0.3067409965488004 2314 66-67 -0.5893227470401747 2314 68-69 -0.3234936852189314 2314 70-71 -0.27558065186942216 2314 72-73 -0.06442663359927181 2314 74-75 -0.12175026581747161 2314 76-77 0.3316512747141829 2315 1 -1.6366001725675545 2315 2 -1.3707467681575025 2315 3 -1.1338168718119377 2315 4 -1.543690526161683 2315 5 -1.2355023169169144 2315 6 -1.097500707855339 2315 7 -1.1086990055934223 2315 8 -1.1989024763650704 2315 9 -1.1156811442093897 2315 10-11 -1.4148265874600625 2315 12-13 -1.76059854355087 2315 14-15 -2.0452183441932164 2315 16-17 -2.448536399798421 2315 18-19 -2.257921192652841 2315 20-21 -2.24162900953063 2315 22-23 -1.6586208266278781 2315 24-25 -2.207620328302422 2315 26-27 -2.1151590631870505 2315 28-29 -2.0774030218401975 2315 30-31 -1.8479048489639176 2315 32-33 -1.939322684501974 2315 34-35 -1.7159375170906337 2315 36-37 -2.366721643713767 2315 38-39 -2.609537952312074 2315 40-41 -2.4559441676225795 2315 42-43 -2.7124780064194915 2315 44-45 -2.513466254694624 2315 46-47 -3.0482241982231564 2315 48-49 -3.699780110014114 2315 50-51 -3.2441808776476897 2315 52-53 -3.047124994698848 2315 54-55 -3.0165422586064956 2315 56-57 -2.6305440381881304 2315 58-59 -2.767951313269535 2315 60-61 -2.696821502863134 2315 62-63 -2.67278642433363 2315 64-65 -2.9996289275832844 2315 66-67 -3.200006244577118 2315 68-69 -3.572800951228782 2315 70-71 -3.263265380933465 2315 72-73 -3.6682597616780903 2315 74-75 -4.033157285521904 2315 76-77 -3.857926927256262 2316 1 -2.3803416284800676 2316 2 -2.173164744917578 2316 3 -2.503028252536481 2316 4 -2.537645584261476 2316 5 -3.3872880312026297 2316 6 -2.869437652489651 2316 7 -3.5276796959556904 2316 8 -3.618395811976825 2316 9 -3.3221490184404203 2316 10-11 -3.5587517412536407 2316 12-13 -3.6581335743095806 2316 14-15 -3.936580271739359 2316 16-17 -5.243873464049955 2316 18-19 -4.690892826282372 2316 20-21 -4.192457786017304 2316 22-23 -4.047141845083104 2316 24-25 -3.4266266509271652 2316 26-27 -3.2214261513620315 2316 28-29 -3.8586530218401975 2316 30-31 -3.8829462877882506 2316 32-33 -3.414021505417903 2316 34-35 -3.6270256066326674 2316 36-37 -3.9707779492503406 2316 38-39 -3.85954863242144 2316 40-41 -3.953402301593872 2316 42-43 -3.815573102113273 2316 44-45 -3.6254044809420627 2316 46-47 -3.212131836637372 2316 48-49 -3.5393328270339346 2316 50-51 -3.727915071085828 2316 52-53 -4.249288784856059 2316 54-55 -3.8542686769250203 2316 56-57 -3.884880162590047 2316 58-59 -3.492190889482792 2316 60-61 -3.2743056963012727 2316 62-63 -3.1768769062201656 2316 64-65 -3.4848262960043392 2316 66-67 -2.88970634710617 2316 68-69 -2.528296935507658 2316 70-71 -2.534283836162441 2316 72-73 -2.3939411526555325 2316 74-75 -2.5117436662464456 2316 76-77 -2.488149500735414 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5491.0 3 784.0 4 1619.0 5 2004.0 6 2319.0 7 2491.0 8 3392.0 9 3835.0 10 6048.0 11 7464.0 12 4734.0 13 2488.0 14 2115.0 15 1880.0 16 1892.0 17 1864.0 18 1823.0 19 1927.0 20 1919.0 21 1982.0 22 2070.0 23 2187.0 24 2266.0 25 2451.0 26 2648.0 27 3054.0 28 3453.0 29 3695.0 30 4128.0 31 4797.0 32 5723.0 33 7242.0 34 12149.0 35 13444.0 36 12789.0 37 18599.0 38 20989.0 39 8311.0 40 170.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 15.40235259114431 29.10720547450297 41.61610472962203 13.874337204730686 2 15.238990903681035 28.99876732126158 42.20436963359687 13.55787214146051 3 14.504542797938472 29.779501620530258 41.55995961957388 14.155995961957387 4 14.584473478669763 30.02704469015499 41.693985877252175 13.694495953923076 5 14.406550025768677 30.75079829767338 41.33614574976224 13.506505926795706 6 14.698084637249952 30.597986345402862 41.40530775974285 13.298621257604335 7 15.05674572813058 30.47957578848933 41.42597551644989 13.037702966930203 8 14.451564226812888 31.254781943381793 41.00410184476749 13.289551985037832 9 14.147720329211985 30.745933998204105 41.10792903558316 13.99841663700075 10-11 14.256034771334903 31.467420470667 40.80212113644455 13.474423621553552 12-13 14.35938035487993 31.710453256962506 40.370182434532644 13.559983953624913 14-15 14.44798639889491 31.76575284241845 40.01965784720009 13.766602911486558 16-17 14.687889324829396 32.00319827441938 39.711995919809375 13.596916480941845 18-19 14.415170731836913 32.527118108228024 39.03493274602892 14.02277841390615 20-21 14.624192991312201 32.667711682031936 38.393421716836265 14.314673609819604 22-23 14.992003060622647 33.17773397025457 36.77741940626046 15.05284356286232 24-25 15.412755805520058 33.61689013812103 35.381725729193505 15.588628327165397 26-27 15.659862535833938 34.16890504238966 34.47736251590774 15.693869905868663 28-29 15.236506410086124 34.511127994517025 34.412572588314674 15.839793007082175 30-31 15.058482250776354 34.47464263798384 34.07165569986106 16.395219411378736 32-33 14.876988101278602 34.45050434745648 34.040597512930795 16.631910038334123 34-35 14.710570358366763 33.94920702388226 34.26666312461813 17.073559493132855 36-37 14.620170442470354 33.8167318400136 34.42773196752667 17.135365749989372 38-39 14.692372473851858 33.48901339751815 34.81574759639013 17.002866532239853 40-41 14.42635928687747 33.35210624048625 34.80868902236544 17.412845450270833 42-43 14.430459107500546 33.66159617029642 34.637618017883995 17.270326704319043 44-45 14.304537943712708 33.83028802473793 34.36903933309601 17.496134698453346 46-47 14.259829486733281 33.61300555536367 34.314665788152276 17.812499169750765 48-49 14.310042960416371 33.296580967233716 34.385867962475395 18.00750810987452 50-51 14.33585851204885 33.15406424458539 34.24721597053752 18.262861272828246 52-53 14.316273384353742 32.931281887755105 34.356664540816325 18.39578018707483 54-55 14.171500235146576 32.80573704501795 34.3335255246957 18.689237195139775 56-57 14.096998230502313 32.987847323701175 34.23420072374049 18.680953722056017 58-59 14.369173465673759 32.69660443726538 33.7707191494223 19.16350294763857 60-61 14.275011357628475 32.50646255702061 33.641428377864976 19.577097707485937 62-63 14.188736795119775 32.07510574531851 33.988883456968566 19.74727400259315 64-65 14.206618047529856 31.803823111359076 33.96644482525007 20.023114015860997 66-67 14.455270338850198 31.59811989021179 33.71523616952872 20.231373601409285 68-69 14.502632131401953 31.20105444698667 33.86108830196301 20.435225119648376 70-71 14.369545111463106 30.930347518258262 33.799579023462 20.90052834681663 72-73 14.411947662517877 30.45851122087119 33.69876705992439 21.430774056686534 74-75 14.621625788888828 30.107241029131064 33.72748975175589 21.543643430224215 76-77 14.470361174174956 29.68375327651384 34.40538916743318 21.44049638187802 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 14776.0 1 8371.5 2 1692.0 3 1357.5 4 1298.0 5 1289.5 6 1229.5 7 1242.0 8 1349.0 9 1392.0 10 1447.5 11 1481.5 12 1509.0 13 1558.0 14 1668.0 15 1890.5 16 2091.0 17 2179.0 18 2405.5 19 2840.0 20 3255.5 21 3637.5 22 3812.0 23 3933.0 24 4397.0 25 4889.0 26 5038.0 27 5212.0 28 5742.0 29 6030.0 30 5962.0 31 6196.5 32 6573.0 33 6777.5 34 6681.0 35 6522.0 36 6496.5 37 6329.0 38 6098.0 39 6009.0 40 5889.0 41 5664.0 42 5444.5 43 5330.0 44 5305.5 45 5216.5 46 5161.5 47 5331.5 48 5492.0 49 5160.5 50 4636.5 51 4023.0 52 3602.0 53 3272.0 54 2684.0 55 2087.0 56 1521.5 57 1295.0 58 1185.0 59 936.0 60 712.5 61 628.0 62 580.5 63 451.0 64 321.5 65 274.0 66 229.0 67 172.5 68 152.5 69 122.0 70 100.0 71 92.0 72 71.5 73 55.5 74 52.0 75 59.5 76 55.0 77 33.0 78 23.0 79 22.5 80 18.5 81 19.0 82 15.5 83 8.0 84 7.5 85 5.5 86 5.0 87 6.0 88 4.5 89 2.5 90 1.0 91 0.0 92 1.0 93 1.5 94 0.5 95 0.5 96 1.0 97 0.5 98 0.0 99 2.5 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009562464140759473 2 0.01487494421895918 3 0.013812448203319238 4 0.01540619222677915 5 0.012218704179859326 6 0.011156208164219385 7 0.01487494421895918 8 0.01487494421895918 9 0.01540619222677915 10-11 0.018593680273698975 12-13 0.016734312246329076 14-15 0.008499968125119531 16-17 0.005046856074289722 18-19 0.016734312246329076 20-21 0.0217811683206188 22-23 0.02071867230497886 24-25 0.016734312246329076 26-27 0.02204679232452878 28-29 0.009031216132939501 30-31 0.008765592129029517 32-33 0.011953080175949342 34-35 0.011156208164219385 36-37 0.010624960156399414 38-39 0.015671816230689133 40-41 0.01142183216812937 42-43 0.012218704179859326 44-45 0.012218704179859326 46-47 0.02098429630888884 48-49 0.02098429630888884 50-51 0.03426549650438811 52-53 0.04037484859431777 54-55 0.029484264434008374 56-57 0.02496865636753862 58-59 0.01832805626978899 60-61 0.01939055228542893 62-63 0.025499904375358595 64-65 0.02045304830106887 66-67 0.029484264434008374 68-69 0.04276546462950764 70-71 0.05418729679763701 72-73 0.07915595316517562 74-75 0.08234344121209546 76-77 0.08234344121209546 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 188236.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.6616449004158 #Duplication Level Percentage of deduplicated Percentage of total 1 82.42453913115679 52.47281741248131 2 10.264031409979056 13.068502457376017 3 3.489723532216805 6.66484620925833 4 1.5921322714411303 4.054310371959106 5 0.8126077583181426 2.586597327668625 6 0.4436378600520294 1.6945629546607592 7 0.3095769444176294 1.3795724253409618 8 0.19148868644840752 0.9752387807300468 9 0.10219169555025737 0.5855122290502698 >10 0.3551885157453212 3.473892531950488 >50 0.010913609427912956 0.48634482958713726 >100 0.0019842926232569013 0.24317241479356863 >500 0.0 0.0 >1k 0.0 0.0 >5k 9.921463116284506E-4 5.816557838529802 >10k+ 9.921463116284506E-4 6.498072216613596 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10288 5.465479504451858 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9209 4.89226290401411 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 256 0.1359994900019125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0010624960156399414 0.0 2 0.0 0.0 0.0 0.009031216132939501 0.0 3 0.0 0.0 0.0 0.012218704179859326 0.0 4 0.0 0.0 0.0 0.020187424297158887 0.0 5 0.0 0.0 0.0 0.03187488046919824 0.0 6 0.0 0.0 0.0 0.04834356871161733 0.0 7 0.0 0.0 0.0 0.0796872011729956 0.0 8 0.0 0.0 0.0 0.11209332965001381 0.0 9 0.0 0.0 0.0 0.1503431862130517 0.0 10 0.0 0.0 0.0 0.20399923500286873 0.0 11 0.0 0.0 0.0 0.2480928196519263 0.0 12 0.0 0.0 0.0 0.3203425487154423 0.0 13 0.0 0.0 0.0 0.40321723793535774 0.0 14 0.0 0.0 0.0 0.48874816719437303 0.0 15 0.0 0.0 0.0 0.5912790327036274 0.0 16 0.0 0.0 0.0 0.6938098982128817 0.0 17 0.0 0.0 0.0 0.7660596272763978 0.0 18 0.0 0.0 0.0 0.8420280923946535 0.0 19 0.0 0.0 0.0 0.9100278373956098 0.0 20 0.0 0.0 0.0 0.9934337746233451 0.0 21 0.0 0.0 0.0 1.0709959837650609 0.0 22 0.0 0.0 0.0 1.1538706729849764 0.0 23 0.0 0.0 0.0 1.2356828661892518 0.0 24 0.0 0.0 0.0 1.3376824836906862 0.0 25 0.0 0.0 0.0 1.4407445972077604 0.0 26 0.0 0.0 0.0 1.5533691748655942 0.0 27 0.0 0.0 0.0 1.6505875602966489 0.0 28 0.0 0.0 0.0 1.742493465649504 0.0 29 0.0 0.0 0.0 1.8211181708068596 0.0 30 0.0 0.0 0.0 1.899211627956395 0.0 31 0.0 0.0 0.0 1.99430502135617 0.0 32 0.0 0.0 0.0 2.0909921587794047 0.0 33 0.0 0.0 0.0 2.212647952570178 0.0 34 0.0 0.0 0.0 2.322085042181092 0.0 35 0.0 0.0 0.0 2.4378971078858456 0.0 36 0.0 0.0 0.0 2.5770840859346777 0.0 37 0.0 0.0 0.0 2.7104273358974904 0.0 38 0.0 0.0 0.0 2.8427080898446633 0.0 39 0.0 0.0 0.0 2.934613995197518 0.0 40 0.0 0.0 0.0 3.061582269066491 0.0 41 0.0 0.0 0.0 3.192269278990204 0.0 42 0.0 0.0 0.0 3.336768737117236 0.0 43 0.0 0.0 0.0 3.4770182111817083 0.0 44 0.0 0.0 0.0 3.6358613655198795 0.0 45 0.0 0.0 0.0 3.7978920079049705 0.0 46 0.0 0.0 0.0 3.9763913385324807 0.0 47 0.0 0.0 0.0 4.17189060541023 0.0 48 0.0 0.0 0.0 4.365264880256699 0.0 49 0.0 0.0 0.0 4.571389107290847 0.0 50 0.0 0.0 0.0 4.747232197879258 0.0 51 0.0 0.0 0.0 4.932106504600608 0.0 52 0.0 0.0 0.0 5.120168299368878 0.0 53 0.0 0.0 0.0 5.3416987186298055 0.0 54 0.0 0.0 0.0 5.572260354023673 0.0 55 0.0 0.0 0.0 5.819821925667779 0.0 56 0.0 0.0 0.0 6.079602201491745 0.0 57 0.0 0.0 0.0 6.33247625321405 0.0 58 0.0 0.0 0.0 6.578975328842517 0.0 59 0.0 0.0 0.0 6.839818100682122 0.0 60 0.0 0.0 0.0 7.102785864553008 0.0 61 0.0 0.0 0.0 7.385409804713232 0.0 62 0.0 0.0 0.0 7.650502560615398 0.0 63 0.0 0.0 0.0 7.941626468900742 0.0 64 0.0 0.0 0.0 8.231156633162625 0.0 65 0.0 0.0 0.0 8.539280477698208 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCTTGA 40 0.0022539233 35.49389 60 TTGATTT 150 0.0053864773 14.197556 37 TGGGATT 220 0.0050801714 11.29351 34 CTGGGAT 220 0.0050801714 11.29351 33 TACATGG 385 2.7246697E-6 11.063031 2 ACATGGG 400 4.307358E-6 10.648167 3 ATGGGAA 270 0.0019268339 10.519503 5 CATGGGA 270 0.0019311033 10.516707 4 GTACATG 390 3.7986414E-5 10.011098 1 TTTTTGG 1160 8.649913E-7 6.1212626 19 TTTTGGG 775 0.008830454 5.497288 20 >>END_MODULE