SRR936151_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR936151_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	188236
Sequences flagged as poor quality	0
Sequence length	125
%GC	34

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6099	3.240081599694001	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4675	2.483584436558363	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3469	1.8428993391274782	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2920	1.5512441828343144	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1352	0.7182473065726004	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1252	0.6651225057906033	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	958	0.5089355914915319	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	901	0.47865445504579357	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	716	0.380373573599099	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT	650	0.34531120508298097	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	632	0.33574874094222146	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	427	0.2268428993391275	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	406	0.2156866911749081	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	384	0.20399923500286876	No Hit
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	372	0.1976242589090291	No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT	367	0.19496801886992923	RNA PCR Primer, Index 39 (95% over 23bp)
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG	298	0.15831190633035125	RNA PCR Primer, Index 39 (96% over 30bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	291	0.15459317027561148	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	265	0.14078072207229222	No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT	250	0.13281200195499268	RNA PCR Primer, Index 39 (96% over 25bp)
GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	234	0.12431203382987313	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	226	0.12006204976731337	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	220	0.11687456172039354	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	215	0.11421832168129369	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT	201	0.1067808495718141	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	199	0.10571835355617416	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	199	0.10571835355617416	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG	194	0.10306211351707431	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGTA	35	1.3389581E-8	59.497345	98-99
TTCTACG	15	0.00423509	59.497345	98-99
CCTTACG	15	0.00423509	59.497345	98-99
CGTATAG	15	0.00423509	59.497345	100-101
GACGTAT	25	7.4442614E-6	59.497345	98-99
GGCGTAT	15	0.00423509	59.497345	98-99
CGCGATG	15	0.00423509	59.497345	78-79
TAACCCG	15	0.00423509	59.497345	96-97
CCGAGCT	15	0.00423509	59.497345	96-97
GTACTCG	15	0.00423509	59.497345	96-97
CGTACGC	15	0.00423509	59.497345	98-99
TCCGTAT	25	7.4442614E-6	59.497345	98-99
CGAGCCG	25	7.4442614E-6	59.497345	96-97
ACGTCTA	15	0.00423509	59.497345	100-101
CACGTAT	35	1.3389581E-8	59.497345	98-99
ATGCCGG	15	0.00423509	59.497345	96-97
GTACGGT	15	0.00423509	59.497345	98-99
CTTACGC	25	7.4442614E-6	59.497345	98-99
AGCGTAT	20	1.7708351E-4	59.49734	98-99
ACACGTA	20	1.7708351E-4	59.49734	98-99

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content