FastQCFastQC Report
Thu 26 May 2016
SRR936150_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936150_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187212
Sequences flagged as poor quality0
Sequence length77
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102525.4761446915795995No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA93144.975108433220092No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2210.11804798837681345No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTG150.002096895671.030269
GGAACGC458.429237E-539.450681
GAACGCA604.568365E-429.5880092
GACCAGG1106.7602587E-419.36669711
GTAGACC950.00661762318.6871648
ACCAGGC1159.1155234E-418.52466612
TGTAGAC1300.002072781316.3872057
AGACCAG1400.003397976515.21669110
CAGGCTG1450.00429893214.68805214
AACGCAA1951.2430752E-414.56640311
ACGCAAA2051.9012766E-413.85584912
CATTTGA1550.00667906413.74410811
AGTACAT3054.490731E-410.4770655
TACATGG3206.987101E-49.9859527
GTACATG3400.00121777359.3985446
CGCAAAA3050.00529452659.3104613
TCAACGC4750.00294121037.4748659
CAACGCA4950.004399467.172850610
ATCAACG5150.0064636346.89429338
TTTTGGG7400.00531134245.756128320
Produced by FastQC (version 0.11.5)