SRR936150_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR936150_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	187212
Sequences flagged as poor quality	0
Sequence length	125
%GC	34

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5948	3.1771467640963182	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4627	2.471529602803239	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3484	1.8609918167638826	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3013	1.6094053799970087	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1370	0.7317906971775314	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1243	0.6639531653953806	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	937	0.5005021045659466	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	923	0.4930239514561032	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	671	0.3584171954789223	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT	647	0.3455975044334765	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	593	0.31675319958122344	No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT	429	0.22915197743734375	RNA PCR Primer, Index 39 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	404	0.21579813259833772	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	384	0.20511505672713287	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	381	0.20351259534645214	No Hit
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	368	0.19656859603016902	No Hit
GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	279	0.1490289084033075	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	261	0.13941414011922312	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	252	0.13460675597718094	No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG	246	0.1314018332158195	RNA PCR Primer, Index 39 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT	231	0.12338952631241588	RNA PCR Primer, Index 39 (96% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	210	0.11217229664765078	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	202	0.10789906629916886	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT	200	0.10683075871204836	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGTA	25	7.4317213E-6	59.51416	98-99
TCGAGTA	25	7.4317213E-6	59.51416	100-101
AACCGTA	35	1.3358658E-8	59.514156	98-99
ACCGGTA	30	3.1422314E-7	59.514156	98-99
ACACGGT	15	0.0042303354	59.514156	98-99
CAACCGT	20	1.7683498E-4	59.514156	96-97
TTCAACG	35	1.3358658E-8	59.514156	100-101
AGACGTG	15	0.0042303354	59.514156	98-99
TGCGCAG	15	0.0042303354	59.514156	98-99
TCCGTAT	30	3.1422314E-7	59.514156	98-99
CGAGCCG	20	1.7683498E-4	59.514156	96-97
CATCGTA	15	0.0042303354	59.514156	98-99
CTCGAGT	15	0.0042303354	59.514156	98-99
CGGTCTA	20	1.7683498E-4	59.514156	100-101
CCGCGTA	20	1.7683498E-4	59.514156	98-99
GAACCCG	15	0.0042303354	59.514156	96-97
CACCGTA	35	1.3358658E-8	59.514156	98-99
CCCGGTA	85	0.0	59.514156	98-99
CCGCCGT	15	0.0042303354	59.514156	96-97
AATCGGT	15	0.0042303354	59.514156	98-99

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content