FastQCFastQC Report
Thu 26 May 2016
SRR936149_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936149_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3301608
Sequences flagged as poor quality0
Sequence length77
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38700.11721561130212915No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATTCG2408.647476E-917.75118354
CGGTTAC1905.370337E-616.8107974
TCGCGCT2201.2053624E-616.13548337
CGCGACC7900.015.28876571
GACGCGA8000.014.6529469
CGCGGAA2252.655289E-514.2007359
ACGCGAC8700.013.47478570
GGACGCG8700.013.47417368
CGATCGT2658.333498E-613.3928561
ATTCGCG2404.8751932E-513.31257956
AATTCGC3253.5745143E-713.10677755
CTCGCGC2801.4675186E-512.67691936
TAAGGCG11800.012.63413717
ACCCCGG16900.012.39168714
ATCGTAC2305.0830306E-412.34565633
TTCCCCG10900.012.05071344
GCCTCGA10900.012.04980121
CCGGGGT9200.011.96438548
GCGTATA2407.2607194E-411.83304643
CGCGCTT3306.053813E-611.8319738
Produced by FastQC (version 0.11.5)