##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936149_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3301608 Sequences flagged as poor quality 0 Sequence length 77 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5318793145643 39.0 36.0 41.0 30.0 41.0 2 36.3890374023809 39.0 36.0 41.0 29.0 41.0 3 36.293250743274186 39.0 36.0 41.0 29.0 41.0 4 36.18161423161078 39.0 36.0 41.0 29.0 41.0 5 36.10665984574789 39.0 36.0 41.0 29.0 41.0 6 36.018752377629326 39.0 35.0 40.0 29.0 41.0 7 35.8635349805307 39.0 35.0 40.0 28.0 41.0 8 35.69241896675802 39.0 35.0 40.0 27.0 41.0 9 35.604504835219686 39.0 35.0 40.0 27.0 41.0 10-11 35.82455412635298 39.0 35.0 40.0 28.0 41.0 12-13 36.033965722157205 39.0 35.0 41.0 29.0 41.0 14-15 35.989989271894174 39.0 35.0 41.0 28.0 41.0 16-17 35.8367071439129 39.0 35.0 41.0 27.5 41.0 18-19 35.70087136328722 39.0 35.0 41.0 27.0 41.0 20-21 35.53471232805349 39.0 35.0 41.0 27.0 41.0 22-23 35.32116532307894 39.0 35.0 41.0 26.0 41.0 24-25 35.12050991516861 39.0 34.5 41.0 25.5 41.0 26-27 34.18641567987478 38.0 33.5 40.0 23.5 40.5 28-29 34.21396119708942 38.0 33.5 39.5 24.0 40.5 30-31 34.590561175039554 38.0 34.0 40.0 24.0 41.0 32-33 34.424310820666776 38.0 34.0 40.0 23.5 41.0 34-35 34.14627811660257 38.0 33.5 40.0 23.0 41.0 36-37 33.84538246212149 37.5 33.0 40.0 22.0 41.0 38-39 33.559116042849425 37.0 33.0 40.0 22.0 41.0 40-41 33.17372080513495 36.5 32.5 40.0 20.5 41.0 42-43 32.79805870351659 36.0 32.0 39.0 19.5 41.0 44-45 32.42053644769457 35.5 32.0 39.0 18.0 40.5 46-47 32.0285984889787 35.0 31.5 38.5 17.0 40.0 48-49 31.56684803283733 35.0 31.0 37.5 13.0 40.0 50-51 31.129386650383694 35.0 31.0 37.0 8.0 39.0 52-53 30.684195701003873 35.0 30.5 36.5 7.0 39.0 54-55 30.22564141472882 34.5 30.0 36.0 3.5 38.5 56-57 29.780423660228593 34.0 29.5 35.5 2.0 37.0 58-59 29.363062180610175 34.0 29.0 35.0 2.0 37.0 60-61 29.019557591331257 34.0 29.0 35.0 2.0 36.0 62-63 28.691825770957664 34.0 29.0 35.0 2.0 36.0 64-65 28.32556499741944 34.0 29.0 35.0 2.0 36.0 66-67 27.954606815830346 34.0 27.5 35.0 2.0 35.0 68-69 27.6024114613243 34.0 27.0 35.0 2.0 35.0 70-71 27.193235841444533 33.0 26.5 35.0 2.0 35.0 72-73 26.71905204978907 33.0 25.5 35.0 2.0 35.0 74-75 26.141258138458596 33.0 24.5 35.0 2.0 35.0 76-77 24.426828230365324 31.0 19.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.8578420472688748 1101 2 0.7623330774395356 1101 3 0.8418043007822646 1101 4 0.6302529199639935 1101 5 0.8163085359310429 1101 6 0.6913181644939996 1101 7 0.879135299096589 1101 8 0.9254535772750856 1101 9 0.9560043695586771 1101 10-11 0.7719240308047617 1101 12-13 0.861002534732549 1101 14-15 0.8467688870056946 1101 16-17 0.971813557638967 1101 18-19 0.9354695704181637 1101 20-21 0.9231190353390133 1101 22-23 0.9225686671269315 1101 24-25 0.9498064610279755 1101 26-27 0.8130492613553528 1101 28-29 0.8408850691292642 1101 30-31 0.9438777039959234 1101 32-33 0.9477851923978307 1101 34-35 0.8954181723111674 1101 36-37 0.8909652856118058 1101 38-39 0.8794782042433482 1101 40-41 0.8490861181622833 1101 42-43 0.8197303413706862 1101 44-45 0.8064077390836601 1101 46-47 0.797596882730943 1101 48-49 0.7846614506807477 1101 50-51 0.7779945726067616 1101 52-53 0.8229769845919783 1101 54-55 0.7997375344704061 1101 56-57 0.7844658971315752 1101 58-59 0.870601063709298 1101 60-61 0.817502339373668 1101 62-63 0.8207540372574513 1101 64-65 0.6320768395625862 1101 66-67 0.7843810166897036 1101 68-69 0.8526387968273355 1101 70-71 0.9315304873500168 1101 72-73 0.8741021314601696 1101 74-75 0.7438960921682813 1101 76-77 0.9429709350139461 1102 1 0.9696798610575783 1102 2 1.0264928978103 1102 3 1.0272195731076081 1102 4 1.0275870842253667 1102 5 1.1073296617307378 1102 6 0.9231125223362753 1102 7 0.7970837490809117 1102 8 0.9830795015831484 1102 9 1.0788406937815154 1102 10-11 0.765279908081034 1102 12-13 0.9745243220337017 1102 14-15 0.9516946421836536 1102 16-17 0.9685075663661422 1102 18-19 1.046230031288097 1102 20-21 0.9747432362058248 1102 22-23 0.9973608323622116 1102 24-25 1.0486389741129756 1102 26-27 0.9528663129933008 1102 28-29 1.0485399297045603 1102 30-31 1.1028145230764324 1102 32-33 1.0719848969103367 1102 34-35 1.1427798477564153 1102 36-37 1.0253238636737336 1102 38-39 1.18739616957199 1102 40-41 1.1558603782862917 1102 42-43 1.1626393084601574 1102 44-45 1.0963751622842963 1102 46-47 1.0025593464393268 1102 48-49 1.0163966321888687 1102 50-51 1.0332720302742082 1102 52-53 1.0886631602119756 1102 54-55 1.1336784822498949 1102 56-57 1.0873332052946374 1102 58-59 1.1079827590491185 1102 60-61 0.9770263231224625 1102 62-63 0.9972062426901935 1102 64-65 0.9776049279557562 1102 66-67 0.925701177536137 1102 68-69 0.9074287041645732 1102 70-71 0.8503560205586034 1102 72-73 0.9201689114731835 1102 74-75 0.9173683573170663 1102 76-77 1.0630957601885598 1103 1 0.5321028253586206 1103 2 0.4570023146883031 1103 3 0.44665724779260074 1103 4 0.530686796310647 1103 5 0.5873161274581946 1103 6 0.5408001649486991 1103 7 0.6356668538040537 1103 8 0.5789455408162638 1103 9 0.33393269760028943 1103 10-11 0.5120368112682812 1103 12-13 0.5281341863805551 1103 14-15 0.4864314195700814 1103 16-17 0.6385193576188684 1103 18-19 0.7012719446356073 1103 20-21 0.6349745766262345 1103 22-23 0.547411168965958 1103 24-25 0.6274061397186443 1103 26-27 0.4121993502309351 1103 28-29 0.45568589902399737 1103 30-31 0.7602716490587866 1103 32-33 0.6824077133309601 1103 34-35 0.6535596466892812 1103 36-37 0.598915214839586 1103 38-39 0.6236613191517151 1103 40-41 0.7131260131990942 1103 42-43 0.6708211539494329 1103 44-45 0.7506858110101504 1103 46-47 0.5342357929373023 1103 48-49 0.6869052092025605 1103 50-51 0.7074475291621418 1103 52-53 0.6170416826314771 1103 54-55 0.5182949912695456 1103 56-57 0.5387865874512769 1103 58-59 0.5506428323122066 1103 60-61 0.7188888568262861 1103 62-63 0.5849250456746056 1103 64-65 0.4951532193995156 1103 66-67 0.6363639580555862 1103 68-69 0.6427655625838469 1103 70-71 0.6414211402809578 1103 72-73 0.7141717981550961 1103 74-75 0.5996614058627294 1103 76-77 0.5689115693079003 1104 1 0.550859165242727 1104 2 0.5642162037202212 1104 3 0.41191934108083217 1104 4 0.35563446941414867 1104 5 0.4689281144297155 1104 6 0.4096175820966934 1104 7 0.6766977121975657 1104 8 0.6241971116249161 1104 9 0.562852526603983 1104 10-11 0.5336060411498025 1104 12-13 0.8257262175056184 1104 14-15 0.690525299241564 1104 16-17 0.6972695847677883 1104 18-19 0.6372498590489002 1104 20-21 0.6194925871563299 1104 22-23 0.6374120785633934 1104 24-25 0.5708404490526959 1104 26-27 0.4968700489119584 1104 28-29 0.4649003814981185 1104 30-31 0.6751439978777825 1104 32-33 0.6945814428347177 1104 34-35 0.7871928332915914 1104 36-37 0.648452963032959 1104 38-39 0.6449327760843815 1104 40-41 0.6679368519941775 1104 42-43 0.693955682809019 1104 44-45 0.6737839559723326 1104 46-47 0.7376280404022992 1104 48-49 0.7277196875895093 1104 50-51 0.6178751221008589 1104 52-53 0.5169891203215506 1104 54-55 0.6662796819833794 1104 56-57 0.5867895402369605 1104 58-59 0.5278090315913815 1104 60-61 0.316686108359427 1104 62-63 0.40694637954113944 1104 64-65 0.23076439975445595 1104 66-67 0.2712296091119697 1104 68-69 0.18056038785598716 1104 70-71 0.11643981808291315 1104 72-73 0.22237991324681872 1104 74-75 0.06807172807059203 1104 76-77 0.031085889849634896 1105 1 0.1542533481920998 1105 2 0.3198266739304003 1105 3 0.13858795518751066 1105 4 0.3667063951938161 1105 5 0.3221647577606106 1105 6 8.353372305549556E-4 1105 7 0.09402109781808576 1105 8 0.34686420956676045 1105 9 0.22320558486338626 1105 10-11 0.2771044890125225 1105 12-13 0.3612732066904272 1105 14-15 0.09863387375293087 1105 16-17 0.16588450945133815 1105 18-19 0.20987769281557433 1105 20-21 0.3327534315686833 1105 22-23 0.266926744186172 1105 24-25 0.3040314596065201 1105 26-27 0.2199300842694214 1105 28-29 0.3144196417389864 1105 30-31 0.18970648320545536 1105 32-33 0.30839369069494893 1105 34-35 0.29655089359403775 1105 36-37 0.3675228652884215 1105 38-39 0.28403961859621063 1105 40-41 0.5239241557891106 1105 42-43 0.31818501003354527 1105 44-45 0.1974142088616091 1105 46-47 0.06445040962864823 1105 48-49 0.05573077380570979 1105 50-51 0.24616756215055702 1105 52-53 0.18335889108538694 1105 54-55 0.16115690949108696 1105 56-57 0.3380548834722781 1105 58-59 0.3279200829783697 1105 60-61 0.1681187087023588 1105 62-63 0.09337453605460766 1105 64-65 0.2854192643038935 1105 66-67 0.2694070378485769 1105 68-69 0.10966928424685563 1105 70-71 -0.26652233870295916 1105 72-73 -0.24665441077763006 1105 74-75 -0.22459105514839095 1105 76-77 0.01730681548679769 1106 1 0.17984244406603977 1106 2 0.27941866099583734 1106 3 0.07681259238400173 1106 4 0.21663554227448856 1106 5 0.023548017208923966 1106 6 0.2588844339784515 1106 7 0.3613921022529709 1106 8 0.3389040691272669 1106 9 0.28538938866189056 1106 10-11 0.12058107098564363 1106 12-13 0.19224462741407677 1106 14-15 0.1867465463928184 1106 16-17 0.31910561960823003 1106 18-19 0.19579227514007158 1106 20-21 0.14358728437117918 1106 22-23 0.13883188956173598 1106 24-25 0.248791152722859 1106 26-27 0.17313549887716562 1106 28-29 0.2608420820936672 1106 30-31 0.28423827873169216 1106 32-33 0.1604288059190111 1106 34-35 0.1299230199039627 1106 36-37 0.06233495121798427 1106 38-39 0.18256031308858667 1106 40-41 0.10268905875761902 1106 42-43 0.13576616538648523 1106 44-45 -0.034798765471705906 1106 46-47 0.06848755457258449 1106 48-49 0.11621036201157509 1106 50-51 -0.10581716679355324 1106 52-53 -0.04822626128182961 1106 54-55 0.25454810232606917 1106 56-57 0.15030802414274191 1106 58-59 -0.04987535229220441 1106 60-61 -0.10246587024223786 1106 62-63 -0.119694773171787 1106 64-65 -0.04334809109417037 1106 66-67 -0.03978859201113849 1106 68-69 -0.2301389090081578 1106 70-71 -0.3708700949974535 1106 72-73 -0.33152229616801776 1106 74-75 -0.5687779498999213 1106 76-77 -0.5518111214046115 1107 1 0.1585811043765446 1107 2 0.13469583898478277 1107 3 0.0071978606985823035 1107 4 0.06042224816449249 1107 5 0.10952863005799429 1107 6 -0.2655481590067339 1107 7 -0.12008883037047724 1107 8 -0.019164390968541056 1107 9 -0.13062782559237007 1107 10-11 0.04372029938358679 1107 12-13 0.02313977192855532 1107 14-15 0.05908252267130365 1107 16-17 0.12288348048956976 1107 18-19 0.08737883355514242 1107 20-21 0.035026038546092764 1107 22-23 -0.03529666351514038 1107 24-25 -0.3345205572181982 1107 26-27 -0.37921616247251677 1107 28-29 -0.10140935422745656 1107 30-31 0.03331428430176686 1107 32-33 0.1064494576388455 1107 34-35 0.2049526073877601 1107 36-37 0.2142213391545269 1107 38-39 0.14226157418031704 1107 40-41 0.00159865864301878 1107 42-43 -0.03193861782411744 1107 44-45 0.14191016294077485 1107 46-47 0.19847852650363507 1107 48-49 0.3191019979672127 1107 50-51 0.22601332866931045 1107 52-53 0.2270421859900722 1107 54-55 0.11653124392553238 1107 56-57 0.1465080216825676 1107 58-59 0.22149284521377766 1107 60-61 0.2487226705318264 1107 62-63 0.2832452042623963 1107 64-65 0.18087743066883988 1107 66-67 0.2861843129339796 1107 68-69 0.14277942178799918 1107 70-71 0.062450240086185715 1107 72-73 0.08223034597806134 1107 74-75 0.1585079235500082 1107 76-77 0.1262729385279755 1108 1 0.12357998789003233 1108 2 0.013987920317973135 1108 3 0.0024048778990959363 1108 4 -0.021324337722752773 1108 5 -0.14557446744714042 1108 6 -0.06033186779468025 1108 7 -0.1545982881332577 1108 8 0.08489518710624111 1108 9 -0.0014700169371906213 1108 10-11 -0.10470012051099076 1108 12-13 -0.039878534403705146 1108 14-15 0.06793228773089766 1108 16-17 -0.09141644935755266 1108 18-19 0.07473191073544427 1108 20-21 -0.01641175512940407 1108 22-23 0.05091154595317704 1108 24-25 0.10627195139640833 1108 26-27 0.08160284016474861 1108 28-29 0.131663091584322 1108 30-31 0.13990375276221556 1108 32-33 0.0834696084730524 1108 34-35 0.2919615326582061 1108 36-37 0.3212337665507121 1108 38-39 0.2610289767470988 1108 40-41 0.29071403713194144 1108 42-43 0.2869386676164183 1108 44-45 0.24084507711110348 1108 46-47 0.16231222666502276 1108 48-49 0.2807038746945949 1108 50-51 0.2900090205396353 1108 52-53 0.17261411218498068 1108 54-55 0.30624934119707703 1108 56-57 0.3028213758545313 1108 58-59 0.23666096748211274 1108 60-61 0.24961972238204666 1108 62-63 0.15589685789480612 1108 64-65 0.11608874827375715 1108 66-67 0.05780271991557129 1108 68-69 0.04801137508023601 1108 70-71 0.012690716934450563 1108 72-73 0.09321044823866487 1108 74-75 -0.02737912590537661 1108 76-77 -0.08232141414712402 1109 1 0.26383888353664986 1109 2 0.3601627697693388 1109 3 0.06351472214463882 1109 4 0.27824269430127657 1109 5 0.13826409520719096 1109 6 0.21039276097204151 1109 7 0.10493345835094203 1109 8 0.292967354436513 1109 9 0.2717412522320686 1109 10-11 0.19924891198711236 1109 12-13 0.2250298687155876 1109 14-15 0.30293330789106676 1109 16-17 0.39652259457234607 1109 18-19 0.3051232696787949 1109 20-21 0.29773344066633456 1109 22-23 0.41956367433733277 1109 24-25 0.30353004591724186 1109 26-27 0.2921027636527356 1109 28-29 0.3435314174114765 1109 30-31 0.4559898782839156 1109 32-33 0.5265958667956028 1109 34-35 0.5117216599981944 1109 36-37 0.4910540533357377 1109 38-39 0.6040643958078888 1109 40-41 0.5330251424754806 1109 42-43 0.610264179721824 1109 44-45 0.4595807311087796 1109 46-47 0.15735394821530235 1109 48-49 0.4773101168746159 1109 50-51 0.4412449520808863 1109 52-53 0.4514975843901148 1109 54-55 0.6010962571628831 1109 56-57 0.5428313074137847 1109 58-59 0.6290522604151008 1109 60-61 0.619255442690136 1109 62-63 0.5486934019522209 1109 64-65 0.6847430895053286 1109 66-67 0.6047199609224094 1109 68-69 0.7575368955869557 1109 70-71 0.8147186978274519 1109 72-73 0.8631030088706808 1109 74-75 0.9965175633748409 1109 76-77 1.004867962084944 1110 1 -0.6626557546303147 1110 2 -0.5990989079982825 1110 3 -0.7224559945561282 1110 4 -0.516862002925663 1110 5 -0.45506590058460006 1110 6 -0.7966149879287485 1110 7 -0.7865594384900021 1110 8 -0.6234185234094696 1110 9 -0.6107616862555858 1110 10-11 -0.6477046744189394 1110 12-13 -0.6144164258877538 1110 14-15 -0.6518604175308411 1110 16-17 -0.6076408071080408 1110 18-19 -0.6204559026108996 1110 20-21 -0.719697958344014 1110 22-23 -0.6426691409515044 1110 24-25 -0.5615487490851478 1110 26-27 -0.4961853677593382 1110 28-29 -0.4223090263364355 1110 30-31 -0.39608520188963325 1110 32-33 -0.2965066320072296 1110 34-35 -0.22582858451299614 1110 36-37 -0.2560081845584179 1110 38-39 -0.28607480144081165 1110 40-41 -0.21029415141681085 1110 42-43 -0.2100502021799997 1110 44-45 -0.33954624783568477 1110 46-47 -0.4454419683751851 1110 48-49 -0.3003813201546812 1110 50-51 -0.3202091639784932 1110 52-53 -0.36549322285960173 1110 54-55 -0.29285843933850586 1110 56-57 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-0.6298719321403183 2314 28-29 -0.7650022181939349 2314 30-31 -0.7683895797536167 2314 32-33 -0.936270115467579 2314 34-35 -0.9488141281769984 2314 36-37 -1.0705166416219711 2314 38-39 -1.0188328924341121 2314 40-41 -0.8451679603510698 2314 42-43 -0.6547259816268678 2314 44-45 -0.8447769255929813 2314 46-47 -0.92219887632724 2314 48-49 -0.890526540759776 2314 50-51 -0.7265719082673385 2314 52-53 -0.6567965705300516 2314 54-55 -0.7718870463150864 2314 56-57 -0.6106785682054259 2314 58-59 -0.7545863121567855 2314 60-61 -0.7799414295656426 2314 62-63 -0.6580478253044113 2314 64-65 -0.6322619686121094 2314 66-67 -0.6835356598206062 2314 68-69 -0.5423519412730329 2314 70-71 -0.6078796692288293 2314 72-73 -0.6533239555194967 2314 74-75 -0.6560857067930961 2314 76-77 -0.5086651287302857 2315 1 -1.3136126924293592 2315 2 -1.1605289983757388 2315 3 -1.1932428100797878 2315 4 -1.0787439805971104 2315 5 -0.9073253370545658 2315 6 -0.977183834287068 2315 7 -1.1510464135032947 2315 8 -1.058001192202667 2315 9 -1.050102462438467 2315 10-11 -1.0397966467740645 2315 12-13 -1.0762585311509127 2315 14-15 -1.3708795562868232 2315 16-17 -1.2201075801011285 2315 18-19 -1.1162692997400896 2315 20-21 -1.181819170465218 2315 22-23 -1.225311352541091 2315 24-25 -1.4408815507863721 2315 26-27 -1.2369615506407854 2315 28-29 -1.2083403926309657 2315 30-31 -1.391446711724825 2315 32-33 -1.2571392742188223 2315 34-35 -1.1859030130084776 2315 36-37 -1.5078157337343825 2315 38-39 -1.3655139295641519 2315 40-41 -1.3182287606672176 2315 42-43 -1.4719986338652724 2315 44-45 -1.3287939883992195 2315 46-47 -1.1607795547654014 2315 48-49 -1.1543286885757347 2315 50-51 -1.0578221358019917 2315 52-53 -1.1631673323758172 2315 54-55 -1.1850301565102193 2315 56-57 -1.3128958742896266 2315 58-59 -1.2407274717372978 2315 60-61 -1.2930795930532177 2315 62-63 -1.447235753781687 2315 64-65 -1.388253181814374 2315 66-67 -1.3352682719350923 2315 68-69 -1.3109816449180016 2315 70-71 -1.434380710121701 2315 72-73 -2.9201235857947907 2315 74-75 -3.64334925486634 2315 76-77 -3.356876935631938 2316 1 -3.179094951141245 2316 2 -3.1593807381479238 2316 3 -2.758443295631686 2316 4 -2.6787276164234157 2316 5 -3.280329416700056 2316 6 -2.853629845133014 2316 7 -3.1482889281150364 2316 8 -2.951944086935434 2316 9 -2.5908858097342673 2316 10-11 -2.6509570946476586 2316 12-13 -2.7302880664490843 2316 14-15 -2.7429673643261054 2316 16-17 -3.9085408366612455 2316 18-19 -3.6583603561899736 2316 20-21 -3.095981955729002 2316 22-23 -3.129738268594501 2316 24-25 -3.3964853440181173 2316 26-27 -3.1161382617349602 2316 28-29 -3.14516462756513 2316 30-31 -3.2501270510249753 2316 32-33 -3.578821722950515 2316 34-35 -3.537294334134625 2316 36-37 -3.3626245212142436 2316 38-39 -3.299128588281576 2316 40-41 -3.2929417159637104 2316 42-43 -3.5893748217943724 2316 44-45 -3.356610763386943 2316 46-47 -3.073219298377065 2316 48-49 -3.123945302852622 2316 50-51 -3.6088419250542323 2316 52-53 -4.494243345997976 2316 54-55 -3.619367891887695 2316 56-57 -3.5048280924167337 2316 58-59 -3.689545953972395 2316 60-61 -3.4761337991750487 2316 62-63 -3.3889504749434955 2316 64-65 -3.247189933875976 2316 66-67 -3.272231805880171 2316 68-69 -3.3366147389761807 2316 70-71 -3.067137755875443 2316 72-73 -3.0301679318913664 2316 74-75 -3.2166161789634415 2316 76-77 -3.3836495786413856 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 84309.0 3 7030.0 4 8367.0 5 9925.0 6 11313.0 7 12708.0 8 13892.0 9 14226.0 10 15517.0 11 16643.0 12 15060.0 13 14214.0 14 14657.0 15 15220.0 16 16049.0 17 16813.0 18 17858.0 19 19081.0 20 20296.0 21 21993.0 22 24418.0 23 27984.0 24 32157.0 25 37772.0 26 44722.0 27 52673.0 28 62930.0 29 74108.0 30 88652.0 31 106175.0 32 129680.0 33 164513.0 34 217026.0 35 293632.0 36 417776.0 37 553721.0 38 483984.0 39 123675.0 40 839.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 23.20692334371815 26.303102267443535 27.568060412656248 22.921913976182072 2 22.871870741159103 26.504405491180382 27.601514712283727 23.022209055376784 3 22.927275817059193 26.626658980570394 27.17339972537251 23.272665476997904 4 22.967989043821383 26.78605128938361 27.351763066881823 22.894196599913183 5 23.07056415519395 26.793532242610873 27.23715383051868 22.898749771676496 6 23.175209953579245 26.51668648194286 27.18874997160231 23.119353592875587 7 23.109098471223703 26.413717455531234 27.331825368047436 23.145358705197633 8 23.043535422628096 26.53490582784675 27.03555985582909 23.385998893696065 9 22.9541757419519 26.432239048314344 27.295469072336715 23.31811613739704 10-11 23.3612510599032 26.503971967359067 27.10010787610697 23.034669096630758 12-13 23.409289704805403 26.237368898959524 27.140060479336224 23.21328091689885 14-15 23.14964822889195 26.562584482087793 27.138406819844697 23.14936046917556 16-17 23.446990727909533 26.365892422426395 27.21230273219376 22.974814117470306 18-19 23.23657781275016 26.332032471806045 27.05147730964771 23.37991240579609 20-21 23.24864981297179 26.478631644863626 27.129072335851586 23.143646206313 22-23 23.48386379475633 26.57415798738259 26.728342485592254 23.21363573226882 24-25 23.2953460202083 26.572261097153095 26.859737159117298 23.27265572352131 26-27 23.36668269302139 26.684744031243213 26.880423095764588 23.068150179970814 28-29 23.571644067817147 26.562891885375308 26.7571307235711 23.108333323236447 30-31 23.369183292178477 26.579852739120213 26.794295185729965 23.25666878297134 32-33 23.25669380446909 26.838132504810986 26.74959160015497 23.155582090564955 34-35 23.242180992048468 26.54383945475199 26.91235138205225 23.301628171147293 36-37 23.178030496942217 26.693230350088708 27.012518595234713 23.11622055773436 38-39 23.189804282698056 26.573947576479995 27.19011932060863 23.046128820213315 40-41 23.092449737896285 26.6514593855485 26.8962591512144 23.35983172534081 42-43 23.063952027459617 26.785698328016228 26.822564397101186 23.327785247422973 44-45 23.34594400232887 26.49832347471641 27.014321878920004 23.14141064403471 46-47 23.198131977911533 26.351593203804814 27.000417320860663 23.44985749742299 48-49 23.209714369848662 26.4599456744673 26.90252971817494 23.427810237509096 50-51 23.13953918201232 26.818617303641506 26.68867819337824 23.353165320967932 52-53 23.234101619585438 26.598731202025032 26.83719516684751 23.32997201154202 54-55 23.226302925366532 26.669999295603432 26.797169386167617 23.30652839286242 56-57 23.275110327836696 26.72786608128096 26.725230482367618 23.27179310851473 58-59 23.325008853090797 26.692182349739042 26.80319056607374 23.179618231096416 60-61 23.253912822989946 26.708696692051603 26.754970919942256 23.2824195650162 62-63 23.246665684073463 26.634688458624822 26.82782407057463 23.290821786727083 64-65 23.298953549421846 26.69350277222605 26.595328918009304 23.412214760342792 66-67 23.086550440393033 26.79380783642683 26.77928056211311 23.340361161067026 68-69 23.205875334729697 26.600949174112472 26.959877887576273 23.23329760358156 70-71 23.03780405519561 26.541298139594577 27.007160906984577 23.41373689822524 72-73 23.17925985252344 26.317952965074927 27.04004214915204 23.4627450332496 74-75 22.980763182445486 26.506035236047566 26.892590456543797 23.620611124963148 76-77 23.015643120814804 26.291541594912882 27.15717985761563 23.535635426656683 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3718.0 1 2253.5 2 770.0 3 761.5 4 772.0 5 774.0 6 813.0 7 898.0 8 966.5 9 987.0 10 1063.0 11 1205.5 12 1394.0 13 1516.0 14 1600.5 15 1838.5 16 2199.5 17 2407.0 18 2609.5 19 3196.5 20 4135.0 21 5542.0 22 6395.0 23 7908.0 24 11206.0 25 15881.5 26 18772.0 27 21810.5 28 30167.0 29 38418.0 30 41351.0 31 48362.5 32 61262.0 33 74088.0 34 88768.0 35 96510.0 36 101742.5 37 112279.5 38 120417.0 39 123250.0 40 127802.0 41 135938.5 42 143555.0 43 147587.0 44 149370.5 45 153091.0 46 157212.0 47 161325.0 48 163254.0 49 162845.5 50 161257.5 51 156535.0 52 152992.0 53 151652.0 54 145869.0 55 133391.5 56 119059.5 57 112762.0 58 106838.5 59 91271.5 60 73443.5 61 65259.0 62 57289.0 63 42745.0 64 31105.0 65 26039.0 66 22014.5 67 15476.0 68 11049.0 69 7659.5 70 6183.0 71 5233.0 72 3598.5 73 2538.0 74 2162.0 75 1875.0 76 1300.5 77 828.0 78 643.0 79 539.5 80 346.0 81 218.0 82 156.0 83 132.0 84 125.0 85 107.5 86 87.0 87 77.0 88 78.5 89 65.5 90 50.0 91 49.0 92 52.0 93 50.0 94 44.5 95 41.0 96 38.0 97 40.0 98 49.5 99 114.0 100 171.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009480229027794941 2 0.011721561130212915 3 0.012387903106607446 4 0.0138417401460137 5 0.011236948783744163 6 0.008571580878166032 7 0.014265775949173857 8 0.01593163089016019 9 0.015689324716925815 10-11 0.018142674720923867 12-13 0.014553514529889677 14-15 0.0073449058761670075 16-17 0.005906212972587903 18-19 0.01788522441186234 20-21 0.023291680902154346 22-23 0.02097462812060063 24-25 0.019308773179614297 26-27 0.024079175965166063 28-29 0.007874950630117204 30-31 0.007753797543500016 32-33 0.009359075941177753 34-35 0.010843201252238303 36-37 0.010873489523892601 38-39 0.012660497551496121 40-41 0.010252579954979513 42-43 0.014220343541692413 44-45 0.013175398169619167 46-47 0.02394287874272173 48-49 0.02320081608719145 50-51 0.03683053833162507 52-53 0.03995023031201766 54-55 0.02757747134123736 56-57 0.019702520711120157 58-59 0.015174424098802765 60-61 0.019217908364651405 62-63 0.02479095034904204 64-65 0.025169553744720755 66-67 0.027865209921953186 68-69 0.04151007630221395 70-71 0.05501864546003038 72-73 0.08621556526395623 74-75 0.08648815970884491 76-77 0.09091024737037226 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 3301608.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.48820274873215 #Duplication Level Percentage of deduplicated Percentage of total 1 72.9126492348612 38.99966565221446 2 14.066423044465273 15.047753755035934 3 5.357768167588454 8.597321700860231 4 2.591361514964399 5.544290804307099 5 1.4409803925595277 3.8537725697085836 6 0.8705897373783805 2.7939768230316178 7 0.5644867010113691 2.1135365378861337 8 0.41072275956854754 1.7575057789856994 9 0.29591673103981936 1.4245248695939907 >10 1.3833404202572457 12.904301717606662 >50 0.07394253394675847 2.7201255679014524 >100 0.028801829287129658 2.687949183313948 >500 0.002163084090779827 0.8089524982271692 >1k 8.5384898116376E-4 0.7463225413270347 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3870 0.11721561130212915 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.057654330859387E-5 2 0.0 0.0 0.0 1.2115308661718774E-4 6.057654330859387E-5 3 0.0 0.0 0.0 1.5144135827148468E-4 9.086481496289081E-5 4 0.0 0.0 0.0 4.84612346468751E-4 1.2115308661718774E-4 5 0.0 0.0 0.0 9.389364212832051E-4 1.2115308661718774E-4 6 6.057654330859387E-5 0.0 0.0 0.0021807555591093794 1.2115308661718774E-4 7 6.057654330859387E-5 0.0 0.0 0.004755258649724619 2.1201790158007855E-4 8 6.057654330859387E-5 0.0 0.0 0.006905725937179702 3.0288271654296936E-4 9 6.057654330859387E-5 0.0 0.0 0.009177346311251972 3.0288271654296936E-4 10 6.057654330859387E-5 0.0 0.0 0.012145596933373071 3.9374753150586017E-4 11 9.086481496289081E-5 0.0 0.0 0.015022982740531281 3.9374753150586017E-4 12 9.086481496289081E-5 0.0 0.0 0.01899074632724418 3.9374753150586017E-4 13 9.086481496289081E-5 0.0 0.0 0.024684941398252003 3.9374753150586017E-4 14 9.086481496289081E-5 0.0 0.0 0.03210556795355475 5.451888897773448E-4 15 9.086481496289081E-5 0.0 0.0 0.04131320253646102 5.451888897773448E-4 16 9.086481496289081E-5 0.0 0.0 0.04930930625319541 5.451888897773448E-4 17 9.086481496289081E-5 3.0288271654296936E-5 0.0 0.05748713959985559 5.451888897773448E-4 18 9.086481496289081E-5 3.0288271654296936E-5 0.0 0.06584670257644154 5.451888897773448E-4 19 9.086481496289081E-5 3.0288271654296936E-5 0.0 0.07541779641919938 6.057654330859387E-4 20 9.086481496289081E-5 3.0288271654296936E-5 0.0 0.08486773717534002 6.057654330859387E-4 21 9.086481496289081E-5 3.0288271654296936E-5 0.0 0.09734650509691035 6.966302480488296E-4 22 9.086481496289081E-5 3.0288271654296936E-5 0.0 0.11055219153818382 7.269185197031265E-4 23 9.086481496289081E-5 3.0288271654296936E-5 0.0 0.12306124773140845 8.480716063203142E-4 24 9.086481496289081E-5 3.0288271654296936E-5 0.0 0.1353279977513987 9.086481496289081E-4 25 9.086481496289081E-5 3.0288271654296936E-5 0.0 0.1472615767831917 9.086481496289081E-4 26 9.086481496289081E-5 3.0288271654296936E-5 0.0 0.1612244700158226 9.389364212832051E-4 27 9.086481496289081E-5 3.0288271654296936E-5 0.0 0.17376381448070152 9.69224692937502E-4 28 9.086481496289081E-5 3.0288271654296936E-5 0.0 0.18748440154009804 0.0012115308661718774 29 9.086481496289081E-5 3.0288271654296936E-5 0.0 0.20184104230423477 0.0013932604960976592 30 9.086481496289081E-5 3.0288271654296936E-5 0.0 0.21710633121800044 0.0013932604960976592 31 9.086481496289081E-5 3.0288271654296936E-5 0.0 0.2334014213680122 0.001423548767751956 32 9.086481496289081E-5 3.0288271654296936E-5 0.0 0.251846978805479 0.001423548767751956 33 9.086481496289081E-5 3.0288271654296936E-5 0.0 0.2696867708098599 0.001423548767751956 34 9.086481496289081E-5 3.0288271654296936E-5 0.0 0.2904342368930533 0.001423548767751956 35 9.086481496289081E-5 3.0288271654296936E-5 0.0 0.310091325196692 0.00148412531106055 36 1.2115308661718774E-4 3.0288271654296936E-5 0.0 0.3310810974531198 0.001514413582714847 37 1.2115308661718774E-4 3.0288271654296936E-5 0.0 0.3548876789733972 0.001514413582714847 38 1.2115308661718774E-4 3.0288271654296936E-5 0.0 0.37633177530463946 0.0016052783976777377 39 1.2115308661718774E-4 6.057654330859387E-5 0.0 0.39862394324220196 0.0016052783976777377 40 1.2115308661718774E-4 6.057654330859387E-5 0.0 0.42452041550662584 0.0016052783976777377 41 1.2115308661718774E-4 6.057654330859387E-5 0.0 0.4509317883891728 0.0016052783976777377 42 1.2115308661718774E-4 6.057654330859387E-5 0.0 0.4805840063387295 0.0016052783976777377 43 1.2115308661718774E-4 6.057654330859387E-5 0.0 0.5119323675009269 0.0016355666693320346 44 1.2115308661718774E-4 6.057654330859387E-5 0.0 0.546430708915171 0.0016355666693320346 45 1.2115308661718774E-4 6.057654330859387E-5 0.0 0.5807170324278351 0.0016355666693320346 46 1.2115308661718774E-4 6.057654330859387E-5 0.0 0.616608634338177 0.0016355666693320346 47 1.2115308661718774E-4 6.057654330859387E-5 0.0 0.6545901269926654 0.0017264314842949254 48 1.2115308661718774E-4 6.057654330859387E-5 0.0 0.6943586276747573 0.001908161114220707 49 1.2115308661718774E-4 6.057654330859387E-5 0.0 0.7351569295930952 0.0022413321024179734 50 1.2115308661718774E-4 6.057654330859387E-5 0.0 0.7778028160823454 0.0022413321024179734 51 1.2115308661718774E-4 6.057654330859387E-5 0.0 0.8228111877606306 0.002332196917380864 52 1.5144135827148468E-4 6.057654330859387E-5 0.0 0.8725748180886405 0.002362485189035161 53 1.5144135827148468E-4 6.057654330859387E-5 0.0 0.9266090947199062 0.002423061732343755 54 1.5144135827148468E-4 6.057654330859387E-5 0.0 0.9853986300008966 0.002423061732343755 55 1.5144135827148468E-4 6.057654330859387E-5 0.0 1.0458843084945275 0.002423061732343755 56 1.5144135827148468E-4 6.057654330859387E-5 0.0 1.1081872832874162 0.002423061732343755 57 1.5144135827148468E-4 6.057654330859387E-5 0.0 1.1728830315409946 0.002544214818960943 58 1.5144135827148468E-4 6.057654330859387E-5 0.0 1.2433638396805435 0.0027865209921953183 59 1.5144135827148468E-4 6.057654330859387E-5 0.0 1.3085744885522448 0.0027865209921953183 60 1.5144135827148468E-4 6.057654330859387E-5 0.0 1.3821749886721864 0.002847097535503912 61 1.5144135827148468E-4 6.057654330859387E-5 0.0 1.4623783320127648 0.002847097535503912 62 1.5144135827148468E-4 6.057654330859387E-5 0.0 1.54573165560539 0.002847097535503912 63 1.5144135827148468E-4 6.057654330859387E-5 0.0 1.633870526119394 0.003028827165429694 64 1.5144135827148468E-4 6.057654330859387E-5 0.0 1.724462746637396 0.003028827165429694 65 1.5144135827148468E-4 6.057654330859387E-5 0.0 1.8233842418603299 0.003059115437083991 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAATTCG 240 8.647476E-9 17.751183 54 CGGTTAC 190 5.370337E-6 16.810797 4 TCGCGCT 220 1.2053624E-6 16.135483 37 CGCGACC 790 0.0 15.288765 71 GACGCGA 800 0.0 14.65294 69 CGCGGAA 225 2.655289E-5 14.20073 59 ACGCGAC 870 0.0 13.474785 70 GGACGCG 870 0.0 13.474173 68 CGATCGT 265 8.333498E-6 13.392856 1 ATTCGCG 240 4.8751932E-5 13.312579 56 AATTCGC 325 3.5745143E-7 13.106777 55 CTCGCGC 280 1.4675186E-5 12.676919 36 TAAGGCG 1180 0.0 12.634137 17 ACCCCGG 1690 0.0 12.391687 14 ATCGTAC 230 5.0830306E-4 12.345656 33 TTCCCCG 1090 0.0 12.050713 44 GCCTCGA 1090 0.0 12.049801 21 CCGGGGT 920 0.0 11.964385 48 GCGTATA 240 7.2607194E-4 11.833046 43 CGCGCTT 330 6.053813E-6 11.83197 38 >>END_MODULE