FastQCFastQC Report
Thu 26 May 2016
SRR936149_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936149_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3301608
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43630.13214772922769755No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA11600.035.15276100-101
GGTATCA57200.034.6423451
GTATCAA107000.031.0875911
CGGTATC10100.028.290895100-101
TCGGTAT4150.027.254131100-101
CCGTACA6950.024.411072100-101
ATCAACG138050.023.8313753
TCAACGC139450.023.5064414
TATCAAC141800.023.286812
TACGCAG7550.023.25958100-101
GCGTATC4750.023.184883100-101
CGTACAT20650.023.061989100-101
CAACGCA143750.022.7619085
AGTATCA27950.022.150234100-101
GCGTACA7450.021.57418100-101
AACGCAG153100.021.4887086
TCGTATC6700.020.87949100-101
GTCGTAT4200.020.5514498-99
CGAGTAC12500.020.477894100-101
TCGTATA3500.020.40986100-101
Produced by FastQC (version 0.11.5)