FastQCFastQC Report
Thu 26 May 2016
SRR936148_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936148_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3322505
Sequences flagged as poor quality0
Sequence length77
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39070.11759199760421729No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACGT3055.7661964E-1016.290872
TACCCGC3502.4738256E-1015.21310125
TAGAACG3654.638423E-1014.58834612
GACGCAT3453.272362E-914.40879748
AACGTAC3705.675247E-1014.3935951
CGATCGT2001.5599205E-414.1959041
ACCGTCG1800.001132982713.80427539
GGTAACG3404.219146E-813.5696131
GACGTAT2507.155235E-512.77881339
TAACGTT3901.841181E-812.7402963
TAAAACG5255.638867E-1112.17396448
TTGGACG4154.3562977E-811.97713545
GTACCCG4458.951247E-911.96644124
GACGCGA7150.011.92341469
GGACGCA4902.9885996E-911.59442647
GCCTCGA9500.011.585052521
AACGCTC4601.4646503E-811.57501215
ACGTATT3104.161723E-511.450376540
ACCCGCA4702.0132575E-811.32839426
GTAGACC19050.011.1790548
Produced by FastQC (version 0.11.5)