##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936148_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3322505 Sequences flagged as poor quality 0 Sequence length 77 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.51156702548228 39.0 36.0 41.0 30.0 41.0 2 36.3814083048784 39.0 36.0 41.0 29.0 41.0 3 36.243432891748846 39.0 36.0 41.0 29.0 41.0 4 36.14100204514365 39.0 36.0 41.0 29.0 41.0 5 36.06186597160877 39.0 35.0 41.0 28.0 41.0 6 35.981460975980475 39.0 35.0 40.0 28.0 41.0 7 35.84812543547715 39.0 35.0 40.0 27.0 41.0 8 35.729832159771014 39.0 35.0 40.0 27.0 41.0 9 35.906778770837064 39.0 35.0 40.0 28.0 41.0 10-11 36.143674576862935 39.0 35.0 41.0 29.0 41.0 12-13 36.08029649315802 39.0 35.0 41.0 28.5 41.0 14-15 35.935126056996154 39.0 35.0 41.0 27.5 41.0 16-17 35.796811291480374 39.0 35.0 41.0 27.0 41.0 18-19 35.657024443906025 39.0 35.0 41.0 27.0 41.0 20-21 35.48682605443784 39.0 35.0 41.0 26.5 41.0 22-23 35.260717741583534 39.0 35.0 41.0 26.0 41.0 24-25 35.0631124708616 39.0 34.0 41.0 25.0 41.0 26-27 34.113137376768435 38.0 33.0 40.0 23.5 40.5 28-29 34.119919608849344 38.0 33.5 39.5 23.5 40.5 30-31 34.5306268914569 38.0 34.0 40.0 24.0 41.0 32-33 34.397590221835635 38.0 34.0 40.0 23.5 41.0 34-35 34.137081659771766 38.0 33.5 40.0 23.0 41.0 36-37 33.81525851729343 37.5 33.0 40.0 22.0 41.0 38-39 33.49916719463176 37.0 33.0 40.0 21.5 41.0 40-41 33.13476383030274 36.5 32.5 40.0 20.0 41.0 42-43 32.75331805369744 36.0 32.0 39.0 19.0 41.0 44-45 32.38020469495155 35.5 32.0 39.0 18.0 40.5 46-47 31.9608543854712 35.0 31.0 38.5 15.5 40.0 48-49 31.543358098783898 35.0 31.0 37.5 12.0 40.0 50-51 31.093004224222387 35.0 31.0 37.0 7.5 39.0 52-53 30.612461531284378 35.0 30.0 36.5 7.0 39.0 54-55 30.153050033032308 34.0 30.0 36.0 2.0 38.5 56-57 29.72619258661763 34.0 29.5 35.5 2.0 37.0 58-59 29.30845100308352 34.0 29.0 35.0 2.0 37.0 60-61 28.959974176111096 34.0 29.0 35.0 2.0 36.0 62-63 28.601527913426768 34.0 29.0 35.0 2.0 36.0 64-65 28.228547586835838 34.0 28.5 35.0 2.0 36.0 66-67 27.882840356899386 34.0 27.5 35.0 2.0 35.0 68-69 27.531166845497598 33.5 27.0 35.0 2.0 35.0 70-71 27.09216269050009 33.0 26.0 35.0 2.0 35.0 72-73 26.623361289147795 33.0 25.0 35.0 2.0 35.0 74-75 26.05447696843195 33.0 24.5 35.0 2.0 35.0 76-77 24.27949634387307 31.0 19.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.0905103527483604 1101 2 0.9085767873468242 1101 3 1.1129342670137703 1101 4 0.8926308651335191 1101 5 0.9313725426445316 1101 6 1.005679303999372 1101 7 1.0480554764902834 1101 8 0.9837811655329816 1101 9 0.9841430491419345 1101 10-11 0.9452588792498275 1101 12-13 1.1436282035370553 1101 14-15 1.1800889579101437 1101 16-17 1.2252022242758258 1101 18-19 1.2611033554159476 1101 20-21 1.2149877010794938 1101 22-23 1.2240109660791703 1101 24-25 1.3222220341820687 1101 26-27 1.2153115919960271 1101 28-29 1.2960074605464342 1101 30-31 1.3505760255443633 1101 32-33 1.4354999130579316 1101 34-35 1.4720170712556353 1101 36-37 1.4128546211893962 1101 38-39 1.3808070332887539 1101 40-41 1.3898194938619497 1101 42-43 1.3883915069045045 1101 44-45 1.2812562483599166 1101 46-47 1.3429335095751043 1101 48-49 1.2975635317598915 1101 50-51 1.2760145337630213 1101 52-53 1.2519513360462362 1101 54-55 1.330945363243707 1101 56-57 1.3368479256068007 1101 58-59 1.430659279962299 1101 60-61 1.3122958906643696 1101 62-63 1.1967813153092877 1101 64-65 1.2416077400008483 1101 66-67 1.2524306006803059 1101 68-69 1.136984270394958 1101 70-71 1.209087943975323 1101 72-73 1.0975128602128557 1101 74-75 1.2178345038726484 1101 76-77 1.3630861186329604 1102 1 0.937367786333148 1102 2 0.8636350735724321 1102 3 0.9423814560408985 1102 4 0.5435520517830668 1102 5 0.778257889476528 1102 6 0.9108331584525757 1102 7 0.8984337405832576 1102 8 0.8928625722418602 1102 9 0.9602380254503231 1102 10-11 1.025755388609177 1102 12-13 1.1590334949780825 1102 14-15 1.1469339592117649 1102 16-17 1.2223620018804908 1102 18-19 1.2020894057211677 1102 20-21 1.2338450297421417 1102 22-23 0.975811024083292 1102 24-25 1.122700469218941 1102 26-27 1.0091476640280348 1102 28-29 1.215671204900687 1102 30-31 1.3764508633357337 1102 32-33 1.2007729135241405 1102 34-35 1.1211178093729615 1102 36-37 1.1030225263001867 1102 38-39 1.2223569905775236 1102 40-41 1.2247987519436805 1102 42-43 1.2175833194145795 1102 44-45 1.1750347616924515 1102 46-47 1.222823499706486 1102 48-49 1.0710048689034153 1102 50-51 1.0152657559574187 1102 52-53 1.0719508807237652 1102 54-55 1.1697280487158501 1102 56-57 1.195455846436122 1102 58-59 1.3547236170276271 1102 60-61 1.2546210344621365 1102 62-63 1.2574909947721657 1102 64-65 1.0634613874802952 1102 66-67 1.095534692346618 1102 68-69 1.1336474990470116 1102 70-71 1.2564348987093403 1102 72-73 1.0468589775494586 1102 74-75 1.034412201188129 1102 76-77 1.0593872669364366 1103 1 0.40404600207836694 1103 2 0.4086421246741878 1103 3 0.40126725544313047 1103 4 0.45218816724401023 1103 5 0.30081586763514423 1103 6 0.3505060924408312 1103 7 0.4181290395567814 1103 8 0.3370924936335129 1103 9 0.43713659177495856 1103 10-11 0.47742994011725415 1103 12-13 0.41349734360469625 1103 14-15 0.3304570575310848 1103 16-17 0.3160158156554971 1103 18-19 0.3031475089407323 1103 20-21 0.409321940181961 1103 22-23 0.17368333344807496 1103 24-25 0.38548101693723424 1103 26-27 0.3003270715019113 1103 28-29 0.17846060907528738 1103 30-31 0.37705577876631224 1103 32-33 0.38196437940391803 1103 34-35 0.4812579652271296 1103 36-37 0.6238858069457223 1103 38-39 0.5261966050199689 1103 40-41 0.49372358788025394 1103 42-43 0.5510412595196357 1103 44-45 0.5525369295777693 1103 46-47 0.4984867363616985 1103 48-49 0.552770593249619 1103 50-51 0.4510198053530807 1103 52-53 0.46318902304798826 1103 54-55 0.5735206558132759 1103 56-57 0.5157391751790179 1103 58-59 0.46725097715157915 1103 60-61 0.3321982875786844 1103 62-63 0.2525410629474152 1103 64-65 0.31497827460135497 1103 66-67 0.30260555080973717 1103 68-69 0.3664459251282075 1103 70-71 0.3998062791194421 1103 72-73 0.47339345592851245 1103 74-75 0.3531310515227766 1103 76-77 0.40527981768344645 1104 1 0.5415919458404801 1104 2 0.48898118135226554 1104 3 0.544505234942342 1104 4 0.5298518702544897 1104 5 0.48018489254803143 1104 6 0.46285888507034656 1104 7 0.5101443957259448 1104 8 0.49295041174489285 1104 9 0.4872723586132821 1104 10-11 0.5330394026826681 1104 12-13 0.49481356442499447 1104 14-15 0.6085144373616913 1104 16-17 0.6208271120490636 1104 18-19 0.4438881592824586 1104 20-21 0.4936036533086394 1104 22-23 0.5439426414137571 1104 24-25 0.5465982320925562 1104 26-27 0.4085814540436843 1104 28-29 0.5069107967346795 1104 30-31 0.692591995333764 1104 32-33 0.6718975468469566 1104 34-35 0.5877338596447714 1104 36-37 0.582170233794642 1104 38-39 0.6723537781066184 1104 40-41 0.5838297748231795 1104 42-43 0.6306066764754519 1104 44-45 0.630208230326744 1104 46-47 0.587525061159134 1104 48-49 0.4715675787329019 1104 50-51 0.5199139136772679 1104 52-53 0.44625955218963753 1104 54-55 0.3671166454989674 1104 56-57 0.39434250950354155 1104 58-59 0.33027695315469074 1104 60-61 0.3560502004375614 1104 62-63 0.25834402566654546 1104 64-65 0.31096467426955954 1104 66-67 0.36202679877817445 1104 68-69 0.24646837454602277 1104 70-71 0.29556236284234316 1104 72-73 0.20351176506320812 1104 74-75 0.05538023854101226 1104 76-77 0.09830587251309808 1105 1 0.37489151055638104 1105 2 0.55098499786817 1105 3 0.3335890989726735 1105 4 0.522640615108088 1105 5 0.4238681084857632 1105 6 0.42910880727009726 1105 7 0.44136765396051914 1105 8 0.5206982833287981 1105 9 0.4306728451462263 1105 10-11 0.6068506480295568 1105 12-13 0.7274061732029793 1105 14-15 0.7083791642530315 1105 16-17 0.6342656975280292 1105 18-19 0.545001993953953 1105 20-21 0.5360852361695407 1105 22-23 0.5722635862994068 1105 24-25 0.6535153029894403 1105 26-27 0.5825722689797388 1105 28-29 0.7411451562366977 1105 30-31 0.8604538062056761 1105 32-33 0.7188587685558119 1105 34-35 0.7134034383580996 1105 36-37 0.6153644536667429 1105 38-39 0.6290925374608562 1105 40-41 0.777845048832333 1105 42-43 0.8236737806774599 1105 44-45 0.566302115028769 1105 46-47 0.6171179573014314 1105 48-49 0.4554460434923726 1105 50-51 0.3999916212251513 1105 52-53 0.41697785703152945 1105 54-55 0.37800007225880705 1105 56-57 0.35077966875501687 1105 58-59 0.41963202553209555 1105 60-61 0.5883190277660368 1105 62-63 0.4678614125317466 1105 64-65 0.6598712994074489 1105 66-67 0.5718040715716626 1105 68-69 0.43938716168322856 1105 70-71 0.356522331801699 1105 72-73 0.061264112644249025 1105 74-75 -0.06532336702034414 1105 76-77 -0.014008549070215537 1106 1 0.07792766695296649 1106 2 0.13680701612799595 1106 3 0.09932207720179065 1106 4 0.17611970491345375 1106 5 0.23194464450185137 1106 6 0.14746567170976732 1106 7 -0.14065551044963343 1106 8 0.08372870878245209 1106 9 0.08528798535634508 1106 10-11 -0.10664156211547748 1106 12-13 0.15652415506939832 1106 14-15 0.18585216847032626 1106 16-17 0.20508387379890536 1106 18-19 0.21411110204056882 1106 20-21 0.19276741808260311 1106 22-23 0.1905312825464236 1106 24-25 0.13487725698848152 1106 26-27 0.10398339508132892 1106 28-29 0.28580701051810564 1106 30-31 0.3783694109932654 1106 32-33 0.31803703053068233 1106 34-35 0.12892032981506674 1106 36-37 0.3010921007416556 1106 38-39 0.3632896918449191 1106 40-41 0.31618392851716237 1106 42-43 0.4667619019592095 1106 44-45 0.3237948542722009 1106 46-47 0.29887076696507364 1106 48-49 0.23600190670035204 1106 50-51 0.12378618643193917 1106 52-53 0.16413687023048595 1106 54-55 0.09670077414629219 1106 56-57 0.032221876996747056 1106 58-59 -0.05647065016405861 1106 60-61 -0.10373800200253669 1106 62-63 0.007023732691372686 1106 64-65 0.017721820883085826 1106 66-67 -0.09306038952363593 1106 68-69 -0.08674821443668534 1106 70-71 -0.3071047448760602 1106 72-73 -0.2849380664530763 1106 74-75 -0.19058588380334385 1106 76-77 -0.26847504055672644 1107 1 4.5404679605098863E-4 1107 2 0.07250100397343573 1107 3 0.06627018707724375 1107 4 0.13202410644869644 1107 5 0.19474514767101425 1107 6 0.0895384363687981 1107 7 0.013788652617982677 1107 8 0.08077707102380316 1107 9 0.045362881636300756 1107 10-11 0.13251522968013063 1107 12-13 0.028198139320778637 1107 14-15 -0.09453292047820128 1107 16-17 -0.09251779987742026 1107 18-19 -0.03868204294595756 1107 20-21 -0.0848680269403701 1107 22-23 -0.13430090630838976 1107 24-25 -0.09488257027576452 1107 26-27 -0.230831701280529 1107 28-29 -0.14899526302704658 1107 30-31 0.09992759923497374 1107 32-33 0.20778148879885094 1107 34-35 0.22604521531742705 1107 36-37 0.22266600727481745 1107 38-39 0.2735557124377195 1107 40-41 0.1206690188904389 1107 42-43 0.12497827113499937 1107 44-45 -0.010249861968979701 1107 46-47 0.11177019885454342 1107 48-49 0.21061369477549974 1107 50-51 0.10945799861766048 1107 52-53 0.09319576280791253 1107 54-55 0.031706650121616775 1107 56-57 0.08719917509598574 1107 58-59 -0.004161876737718728 1107 60-61 0.025635348323710616 1107 62-63 0.08936414032825724 1107 64-65 0.11917994519454922 1107 66-67 0.099409170074658 1107 68-69 0.023616849776214366 1107 70-71 -0.07577175562930094 1107 72-73 -0.09725431296184439 1107 74-75 -0.2309825362668647 1107 76-77 -0.12413875000916264 1108 1 0.36043948084775934 1108 2 0.41158325305727317 1108 3 0.45820558281246093 1108 4 0.33283388924033375 1108 5 0.4334627671788951 1108 6 0.42561438213905944 1108 7 0.49707431438285 1108 8 0.45869024975350214 1108 9 0.4300334013722491 1108 10-11 0.45970502896992826 1108 12-13 0.46914143747638803 1108 14-15 0.561839966049476 1108 16-17 0.47977848340142515 1108 18-19 0.382775408703786 1108 20-21 0.38712185558341616 1108 22-23 0.4827339527337102 1108 24-25 0.4766610661490702 1108 26-27 0.3050911971158641 1108 28-29 0.5403087757886738 1108 30-31 0.6277413037289463 1108 32-33 0.6212865534385159 1108 34-35 0.6116358116251419 1108 36-37 0.6703851380216861 1108 38-39 0.7839024755578876 1108 40-41 0.5902358480469942 1108 42-43 0.6243084211179237 1108 44-45 0.5381425723061213 1108 46-47 0.38995480607448485 1108 48-49 0.4749745963080301 1108 50-51 0.518675988175815 1108 52-53 0.6300582348137063 1108 54-55 0.5675276043660915 1108 56-57 0.615434339096236 1108 58-59 0.5348399798523928 1108 60-61 0.5603291027580859 1108 62-63 0.5943857204014051 1108 64-65 0.5196619941495975 1108 66-67 0.5864032033691302 1108 68-69 0.546708642791117 1108 70-71 0.5942528167582601 1108 72-73 0.6933092519709412 1108 74-75 0.6864163403365602 1108 76-77 0.8404714600561185 1109 1 -0.4078084650285305 1109 2 -0.3846619936431921 1109 3 -0.44664882134306794 1109 4 -0.3536464293279309 1109 5 -0.3515656480486058 1109 6 -0.34787666788102456 1109 7 -0.25367715670407875 1109 8 -0.13260255702039814 1109 9 -0.07805809410167086 1109 10-11 -0.3048303613446208 1109 12-13 -0.23644075437379541 1109 14-15 -0.23855702907004428 1109 16-17 -0.1648071954859418 1109 18-19 -0.361787251634631 1109 20-21 -0.21540350114464957 1109 22-23 -0.159691645794787 1109 24-25 -0.2168893988493963 1109 26-27 -0.3782994052048849 1109 28-29 -0.185200223416075 1109 30-31 -0.004874861791144269 1109 32-33 -0.0036301099919882063 1109 34-35 -0.13774106286978594 1109 36-37 -0.049691122589493375 1109 38-39 -0.003968166889457336 1109 40-41 -0.03870897339150048 1109 42-43 -0.10153330795375126 1109 44-45 -0.07608662463623972 1109 46-47 -0.12350796300764344 1109 48-49 -0.0674424076895761 1109 50-51 -0.17662548040584625 1109 52-53 -0.14646560149253318 1109 54-55 -0.07361695995855655 1109 56-57 -0.12123007062548297 1109 58-59 -0.06400320157172956 1109 60-61 -0.1527823296499342 1109 62-63 -0.28624232142190564 1109 64-65 -0.17448630148711786 1109 66-67 -0.14452999273349576 1109 68-69 -0.10355303689899387 1109 70-71 -0.156294710337189 1109 72-73 -0.28008098842847673 1109 74-75 -0.35335458723611524 1109 76-77 -0.24332675726557795 1110 1 -0.6937044358940554 1110 2 -0.5116858239635675 1110 3 -0.6611695500610679 1110 4 -0.5401409120919212 1110 5 -0.6040154966681541 1110 6 -0.49961762650147534 1110 7 -0.630386932398423 1110 8 -0.8847770167296076 1110 9 -0.8437071384743291 1110 10-11 -0.6795539988371146 1110 12-13 -0.5389674337176302 1110 14-15 -0.5582117859544837 1110 16-17 -0.5060568936708236 1110 18-19 -0.5394770780450315 1110 20-21 -0.7604743734543291 1110 22-23 -0.7055569733851996 1110 24-25 -0.8972313039175503 1110 26-27 -0.8870962727812071 1110 28-29 -0.6449105476628674 1110 30-31 -0.5189464005976205 1110 32-33 -0.4806532170687561 1110 34-35 -0.4964298333445356 1110 36-37 -0.35427181376559247 1110 38-39 -0.5215858247151246 1110 40-41 -0.42482703567723945 1110 42-43 -0.424202877979873 1110 44-45 -0.42345162565434435 1110 46-47 -0.4662670149576762 1110 48-49 -0.5053085497635479 1110 50-51 -0.5862306005254787 1110 52-53 -0.46538376258447656 1110 54-55 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-0.4456206661684732 2314 24-25 -0.39910660492498806 2314 26-27 -0.39994728495909015 2314 28-29 -0.505875765217958 2314 30-31 -0.661060960501942 2314 32-33 -0.7965007278261069 2314 34-35 -0.8624176547947968 2314 36-37 -1.313870687408695 2314 38-39 -0.9620961829445847 2314 40-41 -0.8204121755267195 2314 42-43 -0.6943071969494454 2314 44-45 -0.6714572617569061 2314 46-47 -0.5304602830074927 2314 48-49 -0.413474339505445 2314 50-51 -0.2686864296264382 2314 52-53 -0.23259502269904786 2314 54-55 -0.401624271857667 2314 56-57 -0.31983063117428756 2314 58-59 -0.34992018246620304 2314 60-61 -0.32507097854061584 2314 62-63 -0.44097772133249435 2314 64-65 -0.33673289480153556 2314 66-67 -0.2870531917189716 2314 68-69 -0.24744639875095586 2314 70-71 -0.1660374932928086 2314 72-73 -0.21642697609864925 2314 74-75 -0.24208825299414727 2314 76-77 -0.376432044420401 2315 1 -1.3009808317038605 2315 2 -1.1667889664275037 2315 3 -1.0775637892957022 2315 4 -0.9964382051567782 2315 5 -1.10460316681133 2315 6 -1.1719069437494554 2315 7 -1.4711425530832543 2315 8 -1.470876894325599 2315 9 -1.0340083972283196 2315 10-11 -1.2497365608537052 2315 12-13 -1.2460744377747872 2315 14-15 -1.2254145186853336 2315 16-17 -1.0869971750311223 2315 18-19 -1.0292095911106642 2315 20-21 -0.9733695061028129 2315 22-23 -1.1146331860531618 2315 24-25 -1.249341369216431 2315 26-27 -1.1020784600525033 2315 28-29 -1.2019868403811955 2315 30-31 -1.2709146732582965 2315 32-33 -1.1570327124257602 2315 34-35 -1.4285182439536115 2315 36-37 -1.3873553676305903 2315 38-39 -1.2225641056502141 2315 40-41 -1.4029929934821723 2315 42-43 -1.4257784701052856 2315 44-45 -1.3921570681510147 2315 46-47 -1.296394769865973 2315 48-49 -1.2582163869837117 2315 50-51 -1.2192426949794388 2315 52-53 -1.2216881856107946 2315 54-55 -1.2662547074023394 2315 56-57 -1.547249454355839 2315 58-59 -1.3724823769563699 2315 60-61 -1.4889088395287295 2315 62-63 -1.3831426544876777 2315 64-65 -1.3347283886462051 2315 66-67 -1.2678540907885463 2315 68-69 -1.4651547218368357 2315 70-71 -1.4359679334514333 2315 72-73 -1.301870003550004 2315 74-75 -1.3816545054072904 2315 76-77 -1.5186260330080081 2316 1 -1.9598777386150985 2316 2 -1.5723923460784377 2316 3 -1.755350313881678 2316 4 -2.7324952924975463 2316 5 -2.1912722198167316 2316 6 -2.2790475585784193 2316 7 -1.9572640917229478 2316 8 -1.6829707329364467 2316 9 -1.919393834598786 2316 10-11 -1.6738534695415268 2316 12-13 -2.208465845281083 2316 14-15 -3.420043295481065 2316 16-17 -2.645250065030467 2316 18-19 -2.15343073029959 2316 20-21 -1.913418210046487 2316 22-23 -2.3220075850825808 2316 24-25 -2.4000519147511383 2316 26-27 -2.180816011417818 2316 28-29 -2.7493771867470436 2316 30-31 -2.8680078615427504 2316 32-33 -2.77969085917071 2316 34-35 -2.8858846204822086 2316 36-37 -2.9531001882342878 2316 38-39 -3.1210445509788443 2316 40-41 -2.714281373009868 2316 42-43 -2.976552063970175 2316 44-45 -2.7715455892622813 2316 46-47 -2.622981050815966 2316 48-49 -2.427421432338356 2316 50-51 -2.610505912534002 2316 52-53 -2.613011320007292 2316 54-55 -2.649181538939281 2316 56-57 -2.443403025639906 2316 58-59 -2.647874483466655 2316 60-61 -2.517082932292407 2316 62-63 -2.4907009044322166 2316 64-65 -2.5282197143462533 2316 66-67 -2.4216844977564485 2316 68-69 -2.449445192485566 2316 70-71 -2.314388987470071 2316 72-73 -2.3055994587780155 2316 74-75 -2.389305413298594 2316 76-77 -2.8111704400692332 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 84459.0 3 7069.0 4 8691.0 5 10743.0 6 11970.0 7 12957.0 8 14447.0 9 14246.0 10 15753.0 11 16849.0 12 15391.0 13 14608.0 14 15082.0 15 15796.0 16 16614.0 17 17136.0 18 17994.0 19 19328.0 20 20807.0 21 22447.0 22 24754.0 23 28301.0 24 33078.0 25 38553.0 26 45484.0 27 53864.0 28 62740.0 29 74737.0 30 88795.0 31 106231.0 32 130220.0 33 163844.0 34 217470.0 35 293773.0 36 418555.0 37 559671.0 38 488073.0 39 121244.0 40 731.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 23.144318912398056 26.282178623112507 27.614652334823823 22.958850129665613 2 22.864128679483926 26.493488277032444 27.636663105815085 23.00571993766854 3 22.95935951953731 26.608493162972803 27.171595581269976 23.260551736219913 4 22.946090955313466 26.72389943866006 27.397043776206974 22.9329658298195 5 23.061649787976602 26.81693381685164 27.213377085579065 22.90803930959269 6 23.23461187198118 26.49420211590711 27.16128538978963 23.10990062232208 7 23.155940899559234 26.389745985626462 27.3492762357395 23.105036879074806 8 22.98901196819759 26.535731046841747 27.087947302241165 23.38730968271949 9 22.931765884507616 26.43880820586674 27.30216537684878 23.327260532776865 10-11 23.40206379537554 26.47245425474349 27.107773890151176 23.0177080597298 12-13 23.42895942503929 26.16844585825812 27.219825114312098 23.18276960239049 14-15 23.161897446092954 26.559441289846998 27.149166815537846 23.129494448522202 16-17 23.44434038322588 26.373759837509986 27.201831624600636 22.9800681546635 18-19 23.25272610232725 26.333431313629813 27.047633926347416 23.366208657695527 20-21 23.273885511369365 26.4581537923422 27.1091519925927 23.158808703695733 22-23 23.4792474344699 26.52933167530749 26.76361662757904 23.227804262643577 24-25 23.31847002504279 26.565001296770085 26.81284017636073 23.303688501826393 26-27 23.337694124701574 26.700565648623563 26.865525174840542 23.096215051834317 28-29 23.591497892232187 26.51686289500893 26.753330212944 23.138308999814882 30-31 23.331394879833944 26.584520184598563 26.815569393625253 23.268515541942232 32-33 23.239374828708804 26.829292152910416 26.74171447268198 23.1896185456988 34-35 23.28636018911267 26.549867683439004 26.858960012750792 23.304812114697533 36-37 23.160293758209953 26.678125982033528 27.005997252413167 23.155583007343353 38-39 23.13485549031156 26.63744107608291 27.140500325935673 23.08720310766985 40-41 23.079073009990346 26.689658099513352 26.87679836922772 23.35447052126858 42-43 23.057432864647414 26.7838516934012 26.796449590500615 23.362265851450776 44-45 23.317803493769876 26.461606356271567 27.032095787797562 23.188494362161 46-47 23.2234254923998 26.35832987524231 26.936293910794646 23.48195072156324 48-49 23.18706538259276 26.467678959046438 26.90409872755552 23.44115693080528 50-51 23.172027188847487 26.82185373989657 26.693872574292527 23.31224649696342 52-53 23.254571136397885 26.60000123441495 26.82406260109595 23.321365028091222 54-55 23.259115735017946 26.647919091397892 26.826639218659025 23.266325954925136 56-57 23.307468439250258 26.74375061333423 26.65133657193876 23.297444375476744 58-59 23.359630263575376 26.71669575614687 26.770835904626644 23.15283807565111 60-61 23.236871646622035 26.722999954543226 26.76550655555947 23.274621843275273 62-63 23.238315724192535 26.609396191367885 26.881202649875892 23.27108543456369 64-65 23.280181731756006 26.680487407652254 26.643324034211375 23.396006826380365 66-67 23.115084277852677 26.763424301505236 26.79702014501303 23.32447127562906 68-69 23.24494461586216 26.593508593374015 26.933672860334845 23.227873930428977 70-71 23.07091514374093 26.534556381893143 26.997287696731583 23.397240777634348 72-73 23.22190570963541 26.290896484923255 27.056495187656694 23.430702617784647 74-75 23.03700586652658 26.48676808484234 26.841091108880754 23.635134939750326 76-77 23.00676602703218 26.25949218200531 27.147286616230453 23.586455174732055 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3758.0 1 2253.5 2 723.5 3 715.5 4 733.0 5 761.0 6 811.5 7 907.0 8 996.5 9 1013.0 10 1072.0 11 1185.0 12 1334.0 13 1429.0 14 1532.0 15 1770.0 16 2126.5 17 2348.0 18 2569.0 19 3223.0 20 4103.5 21 5349.0 22 6147.0 23 7837.0 24 11351.5 25 16082.5 26 18989.0 27 21808.5 28 30089.5 29 38560.0 30 41569.0 31 48721.0 32 61725.0 33 74334.5 34 89217.0 35 97342.0 36 102388.5 37 113229.5 38 121613.5 39 124203.0 40 128700.5 41 136807.0 42 144554.0 43 148692.0 44 150131.0 45 153927.0 46 158210.5 47 162010.5 48 163884.0 49 163733.5 50 161955.5 51 157303.0 52 154278.0 53 152912.5 54 146975.0 55 134481.5 56 120367.5 57 114175.0 58 107740.0 59 91828.5 60 74134.5 61 65917.0 62 57913.0 63 43175.5 64 31376.5 65 26311.0 66 22118.5 67 15427.5 68 11071.5 69 7714.0 70 6214.0 71 5240.5 72 3640.0 73 2567.0 74 2121.0 75 1862.0 76 1327.5 77 863.5 78 675.0 79 554.5 80 364.5 81 253.0 82 174.0 83 137.0 84 128.0 85 98.5 86 69.5 87 61.0 88 61.5 89 61.5 90 54.0 91 47.0 92 42.0 93 35.0 94 35.0 95 32.0 96 27.0 97 32.5 98 36.0 99 76.0 100 118.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0034010483054201573 2 0.008006007515413822 3 0.01125656695776229 4 0.019021792292261412 5 0.014025562038281357 6 0.012972139996779538 7 0.017065437072329465 8 0.018600423475660686 9 0.017727588069844893 10-11 0.021444662987715593 12-13 0.020406289832520942 14-15 0.008472522990936055 16-17 0.006275385590089405 18-19 0.012294940112956941 20-21 0.011316762502990966 22-23 0.014507126400110761 24-25 0.023792289251633934 26-27 0.01438673530965341 28-29 0.008126398605871173 30-31 0.008096300833256836 32-33 0.009375456169366187 34-35 0.010790051482240057 36-37 0.009540993918745043 38-39 0.015966868371906136 40-41 0.011978913500506394 42-43 0.015711037304684268 44-45 0.018675667907196526 46-47 0.017622245865694712 48-49 0.014266344219196057 50-51 0.027765195236726502 52-53 0.03291191435377824 54-55 0.025011249042514613 56-57 0.015018788534554501 58-59 0.017185828162786812 60-61 0.020105312106377567 62-63 0.025282128996043648 64-65 0.020647072013435647 66-67 0.020030067674841723 68-69 0.030744874725545938 70-71 0.057050327990477066 72-73 0.0690141926046763 74-75 0.07984939074583786 76-77 0.07632795134996034 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 3322505.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.45320127799771 #Duplication Level Percentage of deduplicated Percentage of total 1 72.87153591540664 38.952168767230695 2 14.074877253555576 15.046944935948355 3 5.38940444124191 8.642427610987157 4 2.549653968585406 5.4514866708816525 5 1.4697188797585614 3.928058955090384 6 0.8825925117038667 2.8306437104736197 7 0.5756175500688872 2.1538020534086098 8 0.4010928145990969 1.7151755959939314 9 0.30540620139873326 1.4692445239423675 >10 1.3716618084067291 12.743544332837464 >50 0.0758155617143231 2.7526029470944406 >100 0.029571444009275947 2.738488752318801 >500 0.0022039691221493775 0.8308871775932622 >1k 8.47680428722591E-4 0.7445239661992656 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3907 0.11759199760421729 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 6.0195545228675355E-5 6.0195545228675355E-5 3 3.0097772614337677E-5 0.0 0.0 1.504888630716884E-4 1.8058663568602606E-4 4 3.0097772614337677E-5 0.0 0.0 3.912710439863898E-4 1.8058663568602606E-4 5 3.0097772614337677E-5 0.0 0.0 7.825420879727796E-4 1.8058663568602606E-4 6 3.0097772614337677E-5 0.0 0.0 0.0016252797211742346 1.8058663568602606E-4 7 3.0097772614337677E-5 0.0 0.0 0.0034010483054201573 2.4078218091470142E-4 8 3.0097772614337677E-5 0.0 0.0 0.00556808793365247 2.4078218091470142E-4 9 3.0097772614337677E-5 0.0 0.0 0.007855518652342134 2.7087995352903906E-4 10 3.0097772614337677E-5 0.0 0.0 0.011316762502990966 4.2136881660072745E-4 11 3.0097772614337677E-5 0.0 0.0 0.014808104126254136 4.2136881660072745E-4 12 3.0097772614337677E-5 0.0 0.0 0.018961596747032735 4.514665892150651E-4 13 3.0097772614337677E-5 0.0 0.0 0.024920955724671595 4.514665892150651E-4 14 3.0097772614337677E-5 0.0 0.0 0.032415301105641676 5.417599070580781E-4 15 3.0097772614337677E-5 0.0 0.0 0.041233948481642614 6.019554522867536E-4 16 3.0097772614337677E-5 0.0 0.0 0.049510835950585474 6.019554522867536E-4 17 3.0097772614337677E-5 0.0 0.0 0.057426550148156286 6.019554522867536E-4 18 3.0097772614337677E-5 0.0 0.0 0.06588402425278517 6.019554522867536E-4 19 3.0097772614337677E-5 0.0 0.0 0.07551531148937322 6.621509975154289E-4 20 3.0097772614337677E-5 0.0 0.0 0.0858689452687054 6.621509975154289E-4 21 6.0195545228675355E-5 0.0 0.0 0.09655365454679526 9.029331784301302E-4 22 9.029331784301303E-5 0.0 0.0 0.10793061259501491 9.33030951044468E-4 23 9.029331784301303E-5 0.0 0.0 0.12051148154780805 9.631287236588057E-4 24 9.029331784301303E-5 0.0 0.0 0.13255059059354313 0.0010534220415018187 25 9.029331784301303E-5 0.0 0.0 0.14480038404757856 0.0010835198141161562 26 9.029331784301303E-5 0.0 0.0 0.15735115522775736 0.0010835198141161562 27 9.029331784301303E-5 0.0 0.0 0.1703232952245369 0.0010835198141161562 28 9.029331784301303E-5 0.0 0.0 0.18329543522131644 0.0012641064498021823 29 9.029331784301303E-5 0.0 0.0 0.19617728190025296 0.0014145953128738707 30 9.029331784301303E-5 0.0 0.0 0.21146695038833652 0.001504888630716884 31 9.029331784301303E-5 0.0 0.0 0.2290440495951097 0.001504888630716884 32 9.029331784301303E-5 0.0 0.0 0.24571821562345278 0.001504888630716884 33 9.029331784301303E-5 0.0 0.0 0.26477010568832854 0.0015951819485598968 34 9.029331784301303E-5 0.0 0.0 0.2828889648021598 0.0015951819485598968 35 9.029331784301303E-5 0.0 0.0 0.3032651568620664 0.0016252797211742346 36 9.029331784301303E-5 0.0 0.0 0.32436369546471716 0.001655377493788572 37 9.029331784301303E-5 0.0 0.0 0.3468768293802417 0.0016854752664029098 38 9.029331784301303E-5 0.0 0.0 0.3700521142932817 0.001956355219931949 39 9.029331784301303E-5 0.0 0.0 0.3939196479764515 0.001956355219931949 40 9.029331784301303E-5 0.0 0.0 0.4175463994787066 0.001956355219931949 41 9.029331784301303E-5 0.0 0.0 0.443430483927037 0.0019864529925462866 42 9.029331784301303E-5 0.0 0.0 0.471331119140528 0.0020165507651606243 43 9.029331784301303E-5 0.0 0.0 0.5052513088768865 0.00219713740084665 44 9.029331784301303E-5 0.0 0.0 0.5397132585203032 0.00219713740084665 45 9.029331784301303E-5 0.0 0.0 0.5746567725255493 0.00219713740084665 46 9.029331784301303E-5 0.0 0.0 0.6108643929805975 0.002227235173460988 47 9.029331784301303E-5 0.0 0.0 0.647192404526103 0.002347626263918339 48 9.029331784301303E-5 0.0 0.0 0.6851456957927828 0.0025282128996043647 49 9.029331784301303E-5 0.0 0.0 0.724995146734166 0.0026486039900617156 50 9.029331784301303E-5 0.0 0.0 0.7690281880689419 0.0026486039900617156 51 9.029331784301303E-5 0.0 0.0 0.8167632554352815 0.0026787017626760533 52 9.029331784301303E-5 0.0 0.0 0.8675682956082835 0.0026787017626760533 53 1.2039109045735071E-4 0.0 0.0 0.9204500820916748 0.002708799535290391 54 1.2039109045735071E-4 0.0 0.0 0.9748066594331687 0.002708799535290391 55 1.2039109045735071E-4 0.0 0.0 1.033226436077598 0.002708799535290391 56 1.2039109045735071E-4 0.0 0.0 1.0974550828365948 0.002708799535290391 57 1.2039109045735071E-4 0.0 0.0 1.1606604053267038 0.0029495817162050924 58 1.2039109045735071E-4 0.0 0.0 1.229494011295694 0.0033709505328058196 59 1.2039109045735071E-4 0.0 0.0 1.2994412348514148 0.0033709505328058196 60 1.504888630716884E-4 0.0 0.0 1.3735118532553 0.0034010483054201573 61 1.504888630716884E-4 0.0 0.0 1.4511942043729054 0.0034010483054201573 62 1.504888630716884E-4 0.0 0.0 1.5339329812897196 0.0034010483054201573 63 1.504888630716884E-4 0.0 0.0 1.62094564191777 0.003702026031563534 64 1.504888630716884E-4 0.0 0.0 1.7095835822669943 0.003702026031563534 65 1.504888630716884E-4 0.0 0.0 1.8061974323590182 0.003702026031563534 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAACGT 305 5.7661964E-10 16.29087 2 TACCCGC 350 2.4738256E-10 15.213101 25 TAGAACG 365 4.638423E-10 14.588346 12 GACGCAT 345 3.272362E-9 14.408797 48 AACGTAC 370 5.675247E-10 14.39359 51 CGATCGT 200 1.5599205E-4 14.195904 1 ACCGTCG 180 0.0011329827 13.804275 39 GGTAACG 340 4.219146E-8 13.569613 1 GACGTAT 250 7.155235E-5 12.778813 39 TAACGTT 390 1.841181E-8 12.740296 3 TAAAACG 525 5.638867E-11 12.173964 48 TTGGACG 415 4.3562977E-8 11.977135 45 GTACCCG 445 8.951247E-9 11.966441 24 GACGCGA 715 0.0 11.923414 69 GGACGCA 490 2.9885996E-9 11.594426 47 GCCTCGA 950 0.0 11.5850525 21 AACGCTC 460 1.4646503E-8 11.575012 15 ACGTATT 310 4.161723E-5 11.4503765 40 ACCCGCA 470 2.0132575E-8 11.328394 26 GTAGACC 1905 0.0 11.179054 8 >>END_MODULE