FastQCFastQC Report
Thu 26 May 2016
SRR936148_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936148_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3322505
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44100.13273117722922917No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA10400.038.347378100-101
GGTATCA57500.032.289161
GTATCAA105850.031.6510051
CCGTACA7600.027.4125100-101
TCGTACA10200.025.968927100-101
CGGTATC10000.025.893064100-101
CGTACAT19500.025.488604100-101
GCGTACA7750.024.961798100-101
AGTATCA23750.024.812231100-101
TCGTATA2850.024.018572100-101
ATCAACG139550.023.7470863
TCAACGC140250.023.5857894
TATCAAC140550.023.4938222
GCGTATC4100.023.22899100-101
CCCGTAC4150.022.94791298-99
CAACGCA145500.022.8567355
CGCATTA2051.8189894E-1221.77718100-101
AACGCAG155050.021.4102296
CGTATAG3850.020.872152100-101
GCGCGTA1004.900225E-520.83240398-99
Produced by FastQC (version 0.11.5)