FastQCFastQC Report
Thu 26 May 2016
SRR936147_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936147_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences707390
Sequences flagged as poor quality0
Sequence length77
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19550.2763680572244448No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9060.12807645004877083No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGCG850.00349129920.8813769
AGTTAAT1106.795116E-419.3627319
TCGCGCT1000.00892581917.75042337
CTGATAC1000.00893309417.74791317
AGTAATG1000.00893309417.74791341
TAAGGCG3450.017.49069817
CCTCGCG1502.8344104E-416.56589135
ACATAAT4000.015.97086313
CTCTCGC1604.6331654E-415.53052354
AGTGTAC2754.6178684E-815.49127911
TTCGCCG1400.003419945715.21034713
GGACGCG4007.2759576E-1215.09426668
GGTTGAG3352.1518645E-914.83827450
TAAGTCG1450.00430072214.69520367
CGATCGT1450.00431489714.6879291
TCGAGGA1707.343971E-414.6159316
CCGGGGT3705.657057E-1014.39325348
GTACATA3959.822543E-1114.38110747
TACATAA3959.822543E-1114.38110748
GACGCGA3756.87578E-1014.206367569
Produced by FastQC (version 0.11.5)