FastQCFastQC Report
Thu 26 May 2016
SRR936147_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936147_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences707390
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18870.2667552552340293No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17320.24484372128528814No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9730.13754788730403314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21700.044.4317361
TTTACGC355.437981E-542.5193698-99
CCGTACA1400.040.39482100-101
CGTACAT3600.037.205757100-101
CGTATCA4100.037.024265100-101
AGGTATC5050.036.542683100-101
TACGCAG2400.035.965565100-101
TCGAGTA952.910383E-1134.464283100-101
AACGTAT806.9503585E-933.48498-99
CGGTATC3800.032.114445100-101
ATACGTA751.4727993E-731.74779198-99
GTATCAA39450.031.2292351
GTGGTAT5800.030.784441
AGTATCA6200.030.724754100-101
TCGCTAA400.005349763729.764608100-101
CCCGTAT1700.029.76355498-99
ACCGTAC703.0841056E-629.76355298-99
CCGTATC2150.029.072405100-101
CCCCGTA1403.6379788E-1227.63758598-99
ATACACG2050.027.58280896-97
Produced by FastQC (version 0.11.5)