FastQCFastQC Report
Thu 26 May 2016
SRR936146_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936146_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences707142
Sequences flagged as poor quality0
Sequence length77
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19970.2824043827123831No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8850.12515166685050527No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATT400.002256742735.49830217
CTCGCAT400.002258309935.49328232
ACGGTAG552.7564284E-432.2666236
ACGCATT500.006730101528.3966317
AATTCGC950.006640587518.68463955
GACGCCT1000.00893678617.74664133
TAAGGCG3950.017.07513417
ACATAAT3850.016.60130749
AGTGTAC2803.2341632E-916.4813549
TACATAA3900.016.3873148
GTACATA4250.015.873198547
AATTTAC3853.6379788E-1215.67679453
TATTAAG4450.015.15765114
CGATATG1655.8562524E-415.05882140
AACCCGA1951.2545493E-414.56134736
TTACACA4453.6379788E-1214.35886456
TCGTTCG1500.005398958514.20032622
AGTATTA4605.456968E-1213.89358812
TAATTTA4353.092282E-1113.87486352
GTATTAA4759.094947E-1213.45293913
Produced by FastQC (version 0.11.5)