##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936146_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 707142 Sequences flagged as poor quality 0 Sequence length 77 %GC 48 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.609161950499335 39.0 36.0 41.0 30.0 41.0 2 36.46650743415042 39.0 36.0 41.0 30.0 41.0 3 36.31656300997537 39.0 36.0 41.0 29.0 41.0 4 36.22525885889963 39.0 36.0 41.0 29.0 41.0 5 36.12273772453057 39.0 35.0 41.0 29.0 41.0 6 36.02633700162061 39.0 35.0 40.0 29.0 41.0 7 35.85660164436563 39.0 35.0 40.0 28.0 41.0 8 35.704496409490595 38.0 35.0 40.0 27.0 41.0 9 35.84761759307183 39.0 35.0 40.0 28.0 41.0 10-11 36.073176250314646 39.0 35.0 41.0 29.0 41.0 12-13 35.963628945812864 39.0 35.0 41.0 28.5 41.0 14-15 35.77844690316796 39.0 35.0 41.0 27.5 41.0 16-17 35.58858899626949 39.0 35.0 41.0 27.0 41.0 18-19 35.38915239089178 39.0 35.0 41.0 26.5 41.0 20-21 35.126645001993936 39.0 34.5 41.0 25.5 41.0 22-23 34.789009138192895 38.5 34.0 40.5 24.0 41.0 24-25 34.490883019252145 38.0 34.0 40.0 23.0 41.0 26-27 33.45335661012923 37.0 32.5 39.5 21.5 40.5 28-29 33.40663261410014 37.5 32.5 39.5 21.5 40.5 30-31 33.74510847891937 38.0 33.0 40.0 21.5 41.0 32-33 33.55039369744691 37.0 33.0 40.0 20.5 41.0 34-35 33.231078057872395 37.0 33.0 40.0 19.5 41.0 36-37 32.859504880207936 36.0 32.0 39.5 18.0 41.0 38-39 32.49651413718885 36.0 32.0 39.0 16.0 41.0 40-41 32.08384525314576 35.0 31.5 39.0 13.0 41.0 42-43 31.69115609029021 35.0 31.0 38.5 9.5 40.0 44-45 31.302150063212196 35.0 31.0 37.5 7.5 40.0 46-47 30.888508248696866 35.0 31.0 37.0 4.5 39.0 48-49 30.491339787482573 35.0 30.0 36.0 2.0 39.0 50-51 30.074202918225758 34.5 30.0 36.0 2.0 38.5 52-53 29.63773966190666 34.0 29.5 35.0 2.0 37.0 54-55 29.235024224271786 34.0 29.0 35.0 2.0 37.0 56-57 28.861053366933376 34.0 29.0 35.0 2.0 36.5 58-59 28.484030081652627 34.0 29.0 35.0 2.0 36.0 60-61 28.191750737475644 34.0 28.0 35.0 2.0 35.5 62-63 27.87338610915488 34.0 27.0 35.0 2.0 35.0 64-65 27.530485390487343 33.5 27.0 35.0 2.0 35.0 66-67 27.204972268653254 33.0 26.0 35.0 2.0 35.0 68-69 26.869529033772565 33.0 25.0 35.0 2.0 35.0 70-71 26.439847300825008 33.0 25.0 35.0 2.0 35.0 72-73 25.970230165935554 33.0 24.0 35.0 2.0 35.0 74-75 25.410533527919426 33.0 21.5 35.0 2.0 35.0 76-77 23.66433403757661 30.5 9.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.1676155907280759 1101 2 0.7912489392453779 1101 3 1.1730173735238338 1101 4 0.9604976471776467 1101 5 0.6836586383538048 1101 6 0.9662583887281926 1101 7 0.9530587875886454 1101 8 0.7516585395070265 1101 9 1.0371733653795445 1101 10-11 0.9390312320171574 1101 12-13 1.0674049631612235 1101 14-15 1.1326240245262085 1101 16-17 1.005280494270103 1101 18-19 1.2054858493135185 1101 20-21 1.3275103426429524 1101 22-23 1.1802173027710623 1101 24-25 1.2276320541073815 1101 26-27 1.2842316605973778 1101 28-29 0.9950088309293861 1101 30-31 1.0317842527105014 1101 32-33 1.3480062722444828 1101 34-35 1.3189415911374383 1101 36-37 1.2700434173630981 1101 38-39 1.2244970798664099 1101 40-41 1.1471140559778377 1101 42-43 1.2175616227739887 1101 44-45 1.0981003439287171 1101 46-47 1.028586406440116 1101 48-49 1.067722322600229 1101 50-51 1.228139328488556 1101 52-53 1.307654171432592 1101 54-55 1.2064390521527244 1101 56-57 0.9962565380881401 1101 58-59 1.1915387340188985 1101 60-61 0.8313478815511317 1101 62-63 0.7105393524227033 1101 64-65 0.5705641954439962 1101 66-67 0.942425497330948 1101 68-69 0.9967980645601706 1101 70-71 0.6688882079885943 1101 72-73 0.4820818605197381 1101 74-75 0.7080988797815984 1101 76-77 0.9661055702036556 1102 1 0.9307236313316665 1102 2 0.9632039058331827 1102 3 0.8302667349692783 1102 4 0.6595051778800993 1102 5 0.9375370914966297 1102 6 1.2223894549964882 1102 7 0.8622992139828582 1102 8 0.924876477470626 1102 9 0.6947620325677377 1102 10-11 0.9116805972326603 1102 12-13 1.0733487043840384 1102 14-15 1.0693340783438714 1102 16-17 1.131188844018915 1102 18-19 0.9430505294707316 1102 20-21 1.2944445267540132 1102 22-23 1.3587298240954837 1102 24-25 1.3234882827240497 1102 26-27 1.305361690098124 1102 28-29 1.3860905093618001 1102 30-31 1.4429332739076912 1102 32-33 1.5236288040308423 1102 34-35 1.5809292159029908 1102 36-37 1.5620419281878952 1102 38-39 1.5961582171061508 1102 40-41 1.4998600762042216 1102 42-43 1.4356539233611763 1102 44-45 1.5308942439654807 1102 46-47 1.3985651527054053 1102 48-49 1.5165331030976859 1102 50-51 1.698414731649752 1102 52-53 1.7915984121888648 1102 54-55 1.5844444998949285 1102 56-57 1.678566220554515 1102 58-59 2.0971151296493957 1102 60-61 1.8205301547111077 1102 62-63 1.5743540443613675 1102 64-65 1.3016714631845048 1102 66-67 1.3151481431617285 1102 68-69 1.2926349715998207 1102 70-71 1.108697961143669 1102 72-73 0.9041994111092855 1102 74-75 1.1508551734318644 1102 76-77 1.0890143629349716 1103 1 0.17739843015107226 1103 2 0.39146997604176903 1103 3 0.5575337228634893 1103 4 0.4308030378913301 1103 5 0.2935517654476598 1103 6 0.5139474236418522 1103 7 0.30555941055247615 1103 8 0.42301743127744373 1103 9 0.24610620526399885 1103 10-11 0.233959614249315 1103 12-13 0.6333872156138582 1103 14-15 0.5947801254186587 1103 16-17 0.24908777233346768 1103 18-19 0.11594633494843265 1103 20-21 0.45887034190462117 1103 22-23 0.44287731218473425 1103 24-25 0.49295234980754543 1103 26-27 0.208581932394182 1103 28-29 0.22957663246695148 1103 30-31 0.21330668402297448 1103 32-33 0.3975676595618651 1103 34-35 0.2518633837966249 1103 36-37 0.32426665068382476 1103 38-39 0.1429038152590607 1103 40-41 0.10128237619046843 1103 42-43 0.014536556555242441 1103 44-45 0.21673573012014558 1103 46-47 0.11631932569477854 1103 48-49 -0.09857556486020158 1103 50-51 -0.11713722130702209 1103 52-53 0.14095366475534377 1103 54-55 0.2497834911622583 1103 56-57 -0.0019980095478295823 1103 58-59 0.08192709428580613 1103 60-61 0.14306283176016876 1103 62-63 0.024195873238927135 1103 64-65 0.045620262188801775 1103 66-67 0.24243564933412287 1103 68-69 -0.22640756887868108 1103 70-71 -0.029803798693272654 1103 72-73 -0.0754130865514071 1103 74-75 -0.0799134502877159 1103 76-77 -0.009931087756900325 1104 1 0.3016914099613217 1104 2 0.18828256528949794 1104 3 0.24487430220235495 1104 4 0.33659531263062803 1104 5 0.18071368226042495 1104 6 0.09021426793145082 1104 7 0.16948628812409083 1104 8 0.0049783081941114915 1104 9 0.011793198320219744 1104 10-11 -0.0029531329432188613 1104 12-13 0.193101043692387 1104 14-15 0.6351941680258832 1104 16-17 0.29747368338982483 1104 18-19 0.44089493048721806 1104 20-21 0.37821713210536956 1104 22-23 0.2772402198839288 1104 24-25 0.40063483842950376 1104 26-27 0.11409351538426193 1104 28-29 0.2272052327284868 1104 30-31 0.24810466393346786 1104 32-33 0.47193537911962125 1104 34-35 0.1940486804499315 1104 36-37 0.4219496071253559 1104 38-39 0.406682772734257 1104 40-41 0.5385254931164098 1104 42-43 0.3830413828939321 1104 44-45 0.27329845915681616 1104 46-47 0.40042727172176384 1104 48-49 -6.809757505976677E-4 1104 50-51 -0.04702665994579647 1104 52-53 0.13545455695411945 1104 54-55 -0.02979997119274458 1104 56-57 -0.026205445205928157 1104 58-59 0.10356486381114749 1104 60-61 -0.002931407375832862 1104 62-63 0.025515792773955326 1104 64-65 0.18134808921140078 1104 66-67 0.23981251810627313 1104 68-69 -0.07741482899305296 1104 70-71 0.1815515191901369 1104 72-73 0.05484904835439508 1104 74-75 -0.05973149074742423 1104 76-77 -0.14719492240718068 1105 1 0.2215630687667911 1105 2 0.350970222950977 1105 3 0.053537779703539456 1105 4 0.06476401516461294 1105 5 -0.14622121094870977 1105 6 -0.17290305567202324 1105 7 -0.19606044670521783 1105 8 -0.13079881069801047 1105 9 -0.11717584919774993 1105 10-11 -0.015429756032567354 1105 12-13 0.1911761492096744 1105 14-15 0.19212546392785868 1105 16-17 -0.27958912648690415 1105 18-19 -0.13579697691228176 1105 20-21 -0.21142061236070475 1105 22-23 -0.031032245969576877 1105 24-25 -0.052539840996800535 1105 26-27 -0.20002401601396969 1105 28-29 -0.22600296198190506 1105 30-31 -0.18624112889292377 1105 32-33 -0.21440289734572104 1105 34-35 -0.2824448164677804 1105 36-37 -0.15131480388820506 1105 38-39 0.05171681309113296 1105 40-41 0.1853067969503286 1105 42-43 -0.26052915672448407 1105 44-45 -0.3099769672134549 1105 46-47 -0.19068514524412095 1105 48-49 -0.35600443175731655 1105 50-51 -0.284169638382064 1105 52-53 -0.13607957165966766 1105 54-55 -0.37092300133192424 1105 56-57 -0.36446515290796455 1105 58-59 -0.5534765856578581 1105 60-61 -0.5764494173723236 1105 62-63 -0.7779003084153864 1105 64-65 -0.6269925243864201 1105 66-67 -0.5505348566415229 1105 68-69 -0.33774479813664726 1105 70-71 -0.4480978885947948 1105 72-73 -0.988535369719731 1105 74-75 -1.5930970732318919 1105 76-77 -1.0750353985923375 1106 1 0.5875024420381507 1106 2 0.4093005852542504 1106 3 0.6199408551071741 1106 4 0.5021254747412556 1106 5 0.38121892740902297 1106 6 0.46342736420648833 1106 7 -0.004990366564911142 1106 8 0.08423585624029073 1106 9 0.42886994969193637 1106 10-11 0.2829937895836281 1106 12-13 0.36518513537611597 1106 14-15 0.4580787881229682 1106 16-17 0.5886122894211283 1106 18-19 0.4354114166720606 1106 20-21 0.5413369095123457 1106 22-23 0.5892368961371872 1106 24-25 0.4104857821998209 1106 26-27 0.2806473112946293 1106 28-29 0.5505275982916231 1106 30-31 0.707363147618814 1106 32-33 0.647939130587126 1106 34-35 0.47771776715472214 1106 36-37 0.16453410982200722 1106 38-39 0.7157121362100227 1106 40-41 0.6703759868888355 1106 42-43 0.8718916828553951 1106 44-45 0.672902149139464 1106 46-47 0.8042836644763547 1106 48-49 0.7023159656004196 1106 50-51 0.534422956999073 1106 52-53 0.9158422234477648 1106 54-55 0.497183193985439 1106 56-57 0.5537226442411765 1106 58-59 0.5328929189514788 1106 60-61 0.14826342611380738 1106 62-63 0.31320033089122745 1106 64-65 0.21946870201049862 1106 66-67 0.309300433880928 1106 68-69 0.11385989025950494 1106 70-71 -0.09815446734111433 1106 72-73 -0.16902378491693426 1106 74-75 -0.04648105801431157 1106 76-77 0.3243928349771288 1107 1 -0.08762037191598182 1107 2 0.22591825388296627 1107 3 0.1832227245576945 1107 4 0.22902677930073168 1107 5 0.11778591867440724 1107 6 -0.17933336168722036 1107 7 -0.13130752097994502 1107 8 -0.06347887952104969 1107 9 -0.2990878418463936 1107 10-11 0.045724245105958516 1107 12-13 0.26200972240024356 1107 14-15 0.24478958490971792 1107 16-17 0.1820614324337555 1107 18-19 0.37554134118745 1107 20-21 0.41802340922431824 1107 22-23 0.48710202717770557 1107 24-25 0.3761116922772558 1107 26-27 0.2475943615277174 1107 28-29 0.1745277088294941 1107 30-31 0.35029605750706594 1107 32-33 0.40288835756602026 1107 34-35 0.43322308466412807 1107 36-37 0.47662291424862246 1107 38-39 0.5827095662044712 1107 40-41 0.1922050910856612 1107 42-43 0.4371377252806816 1107 44-45 0.03292293876250696 1107 46-47 -0.051777956136408676 1107 48-49 -0.09134808854613041 1107 50-51 0.025242999216036566 1107 52-53 0.16965312290719226 1107 54-55 0.32692617217334075 1107 56-57 0.01559983241277152 1107 58-59 0.0835383963581755 1107 60-61 0.26438665033350617 1107 62-63 0.04162790838996955 1107 64-65 0.02444907090939097 1107 66-67 0.35557116238928543 1107 68-69 0.2788780546080645 1107 70-71 -0.10451145790500505 1107 72-73 -0.1102261393736299 1107 74-75 -0.3445761928598863 1107 76-77 -0.044401898318369604 1108 1 0.21848404528052612 1108 2 0.2604301228724637 1108 3 0.3744450051589894 1108 4 0.40539585046350624 1108 5 0.4274816325356312 1108 6 0.23151294459037075 1108 7 0.4745373389398111 1108 8 0.5172249359118837 1108 9 0.3748283244985373 1108 10-11 0.30436844698208887 1108 12-13 0.4305507507508253 1108 14-15 0.5774410638352023 1108 16-17 0.7697746718227094 1108 18-19 0.5545069690346622 1108 20-21 0.26995867536399487 1108 22-23 0.28306914861076393 1108 24-25 0.20090561088371572 1108 26-27 0.15454337298641008 1108 28-29 0.3784562922056338 1108 30-31 0.3723269406905487 1108 32-33 0.31650930948593725 1108 34-35 0.44245714471848174 1108 36-37 0.2618309044409415 1108 38-39 0.4538184208661704 1108 40-41 0.6036614258377995 1108 42-43 0.6347092216018133 1108 44-45 0.6018167997114361 1108 46-47 0.5516700822284797 1108 48-49 0.5332521952155034 1108 50-51 0.4119223387323032 1108 52-53 0.4931010432020493 1108 54-55 0.5267291920110715 1108 56-57 0.6332389041462179 1108 58-59 0.6542908763207116 1108 60-61 0.6841954556025804 1108 62-63 0.3943148870370017 1108 64-65 0.5177328541879227 1108 66-67 0.5391985884823676 1108 68-69 0.5790897935237496 1108 70-71 0.7820724416724971 1108 72-73 0.7637016371906498 1108 74-75 0.9384465179098704 1108 76-77 1.094600960944124 1109 1 0.10421811863495378 1109 2 0.051165110031114125 1109 3 0.10411670897099867 1109 4 0.33549656130698935 1109 5 0.4373853464283428 1109 6 0.14808742925288243 1109 7 0.268252289738669 1109 8 0.1388561245306974 1109 9 0.09087184081715094 1109 10-11 -0.06513742541236667 1109 12-13 -0.1278769288597985 1109 14-15 0.04206241222546936 1109 16-17 -0.02002246364155269 1109 18-19 0.13526802366091317 1109 20-21 0.2058211622581183 1109 22-23 0.1923312757545972 1109 24-25 -0.11399805491402759 1109 26-27 -0.09398251652984158 1109 28-29 0.16604105957031123 1109 30-31 0.1664930222018839 1109 32-33 0.17068250905115434 1109 34-35 0.18394289418222343 1109 36-37 0.44722927498374077 1109 38-39 0.3336954901517686 1109 40-41 0.6558444560125736 1109 42-43 0.5976847605058246 1109 44-45 0.42360093029887125 1109 46-47 0.3673682767437292 1109 48-49 0.5474806077932968 1109 50-51 0.36349744862928546 1109 52-53 0.41112895751607326 1109 54-55 0.4866718628203195 1109 56-57 0.6225118045607978 1109 58-59 0.41615542167906483 1109 60-61 0.360051786341991 1109 62-63 0.2127795035758986 1109 64-65 0.5446227455895034 1109 66-67 0.2735508936737361 1109 68-69 0.40555452549054394 1109 70-71 0.5075137129184455 1109 72-73 0.3250150143224566 1109 74-75 0.4162689705603846 1109 76-77 0.487809952777603 1110 1 -0.02475846034162288 1110 2 -0.0936141847481835 1110 3 -0.3756368707825857 1110 4 -0.21813129232086226 1110 5 -0.26446650542351335 1110 6 -0.2778754967100383 1110 7 -0.3163223948360212 1110 8 -0.3404164547418844 1110 9 -0.46800261805730514 1110 10-11 -0.42659448686942625 1110 12-13 -0.2151174661003381 1110 14-15 -0.41114179097873915 1110 16-17 -0.5808708744899675 1110 18-19 -0.6404423770494887 1110 20-21 -0.7636732322362789 1110 22-23 -0.660333651910328 1110 24-25 -0.6624070533230295 1110 26-27 -0.46272026026759505 1110 28-29 -0.15098265745340456 1110 30-31 -0.25890472819586563 1110 32-33 -0.14014647677782222 1110 34-35 -0.3792698190304904 1110 36-37 -0.2299278271733627 1110 38-39 -0.48012679867051844 1110 40-41 -0.15372560355748277 1110 42-43 -0.4013134634923965 1110 44-45 -0.3085881768902361 1110 46-47 -0.17336321398775922 1110 48-49 -0.2523547420420549 1110 50-51 -0.01691473178289371 1110 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-0.8938610417696822 2314 8 -1.0266749043455263 2314 9 -0.8597158869947705 2314 10-11 -1.0727749835837344 2314 12-13 -1.1857015478943822 2314 14-15 -1.1995157012387736 2314 16-17 -1.183193456349919 2314 18-19 -1.1317644693502302 2314 20-21 -1.2201989623385217 2314 22-23 -1.2363259507674016 2314 24-25 -1.2897913008140378 2314 26-27 -1.17239046682392 2314 28-29 -1.413979512976951 2314 30-31 -1.48751240297792 2314 32-33 -1.7225501183451684 2314 34-35 -1.4565966374605104 2314 36-37 -2.0970995871387146 2314 38-39 -1.4391238613152666 2314 40-41 -1.4732524257533441 2314 42-43 -1.4695999121169265 2314 44-45 -1.6894817165916045 2314 46-47 -1.3569902828461444 2314 48-49 -1.3056272570583403 2314 50-51 -1.56411953699482 2314 52-53 -1.5119637267865755 2314 54-55 -1.5675417764467028 2314 56-57 -1.513488494072952 2314 58-59 -1.4545510960025432 2314 60-61 -1.4413011904558246 2314 62-63 -1.2693323347636998 2314 64-65 -1.4229796401623496 2314 66-67 -1.529126855623229 2314 68-69 -1.5050328705061027 2314 70-71 -1.6402386533392566 2314 72-73 -1.5737174513139216 2314 74-75 -1.3368198149127473 2314 76-77 -1.3447776257174517 2315 1 -0.9199839886365808 2315 2 -0.9142855172952764 2315 3 -0.8317299048293307 2315 4 -0.9135311466732148 2315 5 -1.0956231797592366 2315 6 -1.2060602501079458 2315 7 -1.429602109211956 2315 8 -1.2125590863242195 2315 9 -0.9214855946827925 2315 10-11 -0.9868932578868339 2315 12-13 -0.8080886781336645 2315 14-15 -0.8529487885104601 2315 16-17 -0.7169015893879163 2315 18-19 -0.6135757026493209 2315 20-21 -0.9916448309447787 2315 22-23 -1.2325122120608754 2315 24-25 -1.0017257961131492 2315 26-27 -0.7762904643676407 2315 28-29 -0.7742611473579615 2315 30-31 -0.8309721192340263 2315 32-33 -1.0387465515350272 2315 34-35 -0.9551532106873282 2315 36-37 -1.1793611869294978 2315 38-39 -1.2538082363288119 2315 40-41 -1.0036489129813972 2315 42-43 -0.8854476977788863 2315 44-45 -1.0245647894868277 2315 46-47 -0.6571276901395713 2315 48-49 -0.8088399291606159 2315 50-51 -0.6971279186952337 2315 52-53 -0.6802298663640656 2315 54-55 -1.0150640383065692 2315 56-57 -0.9125418141712593 2315 58-59 -0.6417826207900355 2315 60-61 -0.6295788023141746 2315 62-63 -0.5942160587180787 2315 64-65 -0.537917734636725 2315 66-67 -0.796349364411661 2315 68-69 -0.8691130724413654 2315 70-71 -0.7844021665735568 2315 72-73 -0.9296471323007118 2315 74-75 -0.9128980864994887 2315 76-77 -0.8988598938191608 2316 1 -2.021294779063375 2316 2 -1.7208628619968636 2316 3 -2.186264433065773 2316 4 -2.970389605506327 2316 5 -2.0367868390021187 2316 6 -2.147054514895679 2316 7 -1.8109122543265386 2316 8 -1.5173287501972226 2316 9 -1.6899830658655688 2316 10-11 -1.6879596363140479 2316 12-13 -2.302813250815994 2316 14-15 -3.792726639782124 2316 16-17 -2.679481547603949 2316 18-19 -1.9950334629319642 2316 20-21 -1.8962628464484013 2316 22-23 -2.605830165453206 2316 24-25 -2.4281924697707 2316 26-27 -2.361407144881632 2316 28-29 -2.8300919464964664 2316 30-31 -2.8668789038852722 2316 32-33 -3.131674736352444 2316 34-35 -3.086742050012653 2316 36-37 -2.9456387841094838 2316 38-39 -3.2039231825662426 2316 40-41 -2.971029994040677 2316 42-43 -2.7472742539896124 2316 44-45 -2.745657625714653 2316 46-47 -2.8770581577489303 2316 48-49 -2.6335086016727374 2316 50-51 -2.50690036547714 2316 52-53 -2.6802895577434995 2316 54-55 -2.923386952536511 2316 56-57 -2.998244922156193 2316 58-59 -3.0424561654093445 2316 60-61 -2.879451756472811 2316 62-63 -2.9811444532619653 2316 64-65 -2.6931209676508985 2316 66-67 -3.008012575969204 2316 68-69 -3.064820132963586 2316 70-71 -2.7090240539504435 2316 72-73 -2.6013639047716914 2316 74-75 -2.7710463631125677 2316 76-77 -3.250878511076426 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16795.0 3 1721.0 4 2356.0 5 2813.0 6 3041.0 7 3224.0 8 3952.0 9 4116.0 10 4880.0 11 5276.0 12 4373.0 13 3730.0 14 3624.0 15 3683.0 16 3702.0 17 3891.0 18 4053.0 19 4281.0 20 4641.0 21 5079.0 22 5667.0 23 6420.0 24 7463.0 25 8699.0 26 10211.0 27 12231.0 28 14449.0 29 16860.0 30 20366.0 31 24135.0 32 29801.0 33 37978.0 34 51403.0 35 70817.0 36 97366.0 37 113869.0 38 75765.0 39 14340.0 40 71.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 24.407807466363465 24.7681433650395 27.352436227051214 23.471612941545825 2 23.890710166121227 25.21942540667596 27.319205627699507 23.570658799503306 3 23.85373912010387 24.882113832637 27.098684936311766 24.16546211094736 4 23.7687329820715 25.189858198663316 27.24056720534672 23.800841613918454 5 23.805657823459274 25.444364453106765 27.020555259320446 23.72942246411351 6 23.96064642601545 25.163289610283073 26.909618599598883 23.966445364102594 7 23.913606178261514 24.968493412258926 26.85818145814327 24.25971895133629 8 24.07971359909248 25.10307914922749 26.416975845178296 24.400231406501728 9 23.91763426229114 24.83320202604552 26.891921136418606 24.357242575244733 10-11 24.523295082836785 25.136128153897836 26.478773626606788 23.86180313665859 12-13 24.65424604597511 24.716766423445424 26.559844519459467 24.069143011119998 14-15 24.217218056012246 24.894583691320964 26.526441585257626 24.36175666740916 16-17 24.42931350739018 24.85832719311768 26.80660229929273 23.905757000199408 18-19 24.478779932762222 25.01884609564058 26.234189434023108 24.268184537574093 20-21 24.34701809171447 24.94682235271489 26.434877182263556 24.271282373307084 22-23 24.737637121730717 25.107561708314712 25.967984657075455 24.186816512879115 24-25 24.464982333454977 25.15898476066004 25.68572786448388 24.690305041401103 26-27 24.478053975615293 25.59093065965718 25.644109999200897 24.286905365526636 28-29 24.9749849909946 25.062316541868583 25.60780284425072 24.3548956228861 30-31 24.57055414284785 25.251175973094753 25.656720512987324 24.521549371070073 32-33 24.480789071908475 25.448389348264676 25.58904249082643 24.481779089000415 34-35 24.535728454271524 25.05867702144902 25.678012187282768 24.727582336996683 36-37 24.32829013199068 25.1703008595482 26.13705391711424 24.364355091346884 38-39 24.288774493406308 25.14075288383405 26.14520619828954 24.425266424470106 40-41 24.269978530028258 25.51383667546398 25.67981172051132 24.536373073996444 42-43 24.070945408385164 25.46722346177102 25.761697824326667 24.700133305517152 44-45 24.279910185463482 25.171690740969925 25.93702374427609 24.6113753292905 46-47 24.22892971278768 25.22960791094459 25.665238843852478 24.876223532415256 48-49 24.300329759932225 25.27772886421288 25.58443459572762 24.83750678012728 50-51 24.135745844670122 25.72361548531834 25.582375644225543 24.558263025785994 52-53 24.546091114207506 25.414300163385978 25.519616927070437 24.519991795336075 54-55 24.368434416124966 25.498777500924742 25.663286199163025 24.469501883787263 56-57 24.509291548181185 25.457010368260296 25.642579667856413 24.391118415702103 58-59 24.397533273928232 25.55869082474081 25.58330150917243 24.460474392158527 60-61 24.3115006495844 25.623187298839234 25.468800500148163 24.5965115514282 62-63 24.471184347608368 25.47530991245994 25.492426090997245 24.56107964893445 64-65 24.32009041414829 25.416036629611767 25.322821792751878 24.941051163488073 66-67 24.07978291172408 25.525057868283092 25.501648883617744 24.893510336375087 68-69 24.09193818637308 25.38046472678046 25.69782220766793 24.829774879178533 70-71 24.154207614165436 25.234436295230907 25.690043912367695 24.92131217823596 72-73 24.23760005603775 24.788637921848565 25.82597307216346 25.147788949950222 74-75 23.92440552176239 25.114211150518123 25.74152332483236 25.219860002887124 76-77 24.011347007859975 24.74958181082282 26.0124282143959 25.226642966921307 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2092.0 1 1246.0 2 377.5 3 367.0 4 379.0 5 383.0 6 403.0 7 445.5 8 500.5 9 529.0 10 537.5 11 567.5 12 620.5 13 652.0 14 702.0 15 794.5 16 925.5 17 1014.0 18 1116.0 19 1300.0 20 1496.5 21 1841.0 22 2071.0 23 2382.0 24 2979.0 25 3638.5 26 4012.0 27 3949.5 28 4960.0 29 6047.5 30 6062.0 31 6722.5 32 7780.0 33 8742.5 34 10664.0 35 12020.0 36 12654.0 37 14093.0 38 15401.5 39 15905.0 40 17414.0 41 20332.5 42 23589.5 43 25437.0 44 26698.0 45 29434.5 46 31866.0 47 33547.5 48 34273.0 49 35080.0 50 36852.5 51 37897.5 52 37977.0 53 38178.0 54 37934.5 55 36212.5 56 33636.5 57 32338.0 58 31311.5 59 27781.0 60 23316.0 61 21355.0 62 19068.5 63 14646.5 64 10680.5 65 8850.0 66 7460.5 67 5310.0 68 3956.5 69 2920.5 70 2477.0 71 2113.5 72 1460.5 73 1049.5 74 928.0 75 833.5 76 602.5 77 377.5 78 289.0 79 241.0 80 158.5 81 107.5 82 78.5 83 66.0 84 50.0 85 31.0 86 27.0 87 26.0 88 29.0 89 28.5 90 23.0 91 21.0 92 18.0 93 15.5 94 15.0 95 14.0 96 14.0 97 9.0 98 10.0 99 24.5 100 33.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0033939435078103124 2 0.009616173272129219 3 0.014707088533844687 4 0.023616190241846757 5 0.01711113185187699 6 0.016404060287749846 7 0.020787903985338165 8 0.02276770436489418 9 0.02107073261098902 10-11 0.02538386915216463 12-13 0.02439396896238662 14-15 0.009121223177240215 16-17 0.0077777872053986325 18-19 0.014000016969717538 20-21 0.011030316400383516 22-23 0.014989917159495547 24-25 0.022060632800767033 26-27 0.014212138438955682 28-29 0.009404051802891075 30-31 0.009616173272129219 32-33 0.011808095120923378 34-35 0.013222238249177676 36-37 0.01194950943374881 38-39 0.01986871095197287 40-41 0.015272745785146407 42-43 0.016899010382638847 44-45 0.018313153510893146 46-47 0.017181839008289707 48-49 0.01590911019286084 50-51 0.026656597967593496 52-53 0.032101049011372534 54-55 0.026656597967593496 56-57 0.018171739198067714 58-59 0.018666689292956718 60-61 0.021424268393052597 62-63 0.02948488422410209 64-65 0.024464676118799333 66-67 0.02100002545457631 68-69 0.02976771284975295 70-71 0.05564653209680658 72-73 0.06724250574849182 74-75 0.0786263579309389 76-77 0.07410109992052516 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 707142.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.04104006328008 #Duplication Level Percentage of deduplicated Percentage of total 1 72.2820246524315 38.339137654446176 2 13.532301664041913 14.355347094216771 3 5.558057399125722 8.84415435543113 4 2.8409205993355666 6.027415333038218 5 1.6629821873431694 4.410315241169509 6 0.9916887297954032 3.156012098842879 7 0.6831834180735962 2.5365731334027166 8 0.48511959144735384 2.058499814835293 9 0.35796912814315146 1.7088349380532504 >10 1.5258968951141283 13.409523955036073 >50 0.052971085335249225 1.8705978177041973 >100 0.025287541903633706 2.566922805146333 >500 0.001330923258009709 0.4347151046036459 >1k 2.6618465160194177E-4 0.28195065407388137 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1997 0.2824043827123831 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 885 0.12515166685050527 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 2.8282862565085935E-4 0.0 3 0.0 0.0 0.0 2.8282862565085935E-4 0.0 4 0.0 0.0 0.0 5.656572513017187E-4 0.0 5 0.0 0.0 0.0 0.001979800379556016 0.0 6 0.0 0.0 0.0 0.0035353578206357423 0.0 7 0.0 0.0 0.0 0.006646472702795195 0.0 8 0.0 0.0 0.0 0.010888902087558086 0.0 9 0.0 0.0 0.0 0.015696988723622695 0.0 10 0.0 0.0 0.0 0.02135356123663988 0.0 11 0.0 0.0 0.0 0.03153539176007082 0.0 12 0.0 0.0 0.0 0.04044449346807289 0.0 13 0.0 0.0 0.0 0.05204046711975813 0.0 14 0.0 0.0 0.0 0.06448492664839593 0.0 15 0.0 0.0 0.0 0.07721221480268461 0.0 16 0.0 0.0 0.0 0.08838394551589356 0.0 17 0.0 0.0 0.0 0.10111123367018222 0.0 18 0.0 0.0 0.0 0.11553549357837606 0.0 19 0.0 0.0 0.0 0.13038399642504617 0.0 20 0.0 0.0 0.0 0.14791937121539944 0.0 21 0.0 0.0 0.0 0.16828303226226132 0.0 22 0.0 0.0 0.0 0.18977800781172663 0.0 23 0.0 0.0 0.0 0.21183864061249366 0.0 24 0.0 0.0 0.0 0.2334750304747844 0.0 25 0.0 0.0 0.0 0.2511518195779631 0.0 26 0.0 0.0 0.0 0.2701013374965707 0.0 27 0.0 0.0 0.0 0.28961651266648 0.0 28 0.0 0.0 0.0 0.3082832019594367 0.0 29 0.0 0.0 0.0 0.328788277319124 0.0 30 0.0 0.0 0.0 0.3519802246224945 0.0 31 0.0 0.0 0.0 0.37644490074129383 0.0 32 0.0 0.0 0.0 0.4068489779987612 0.0 33 0.0 0.0 0.0 0.43810154113318117 0.0 34 0.0 0.0 0.0 0.4710510760215063 0.0 35 0.0 0.0 0.0 0.49947535289941764 0.0 36 0.0 0.0 0.0 0.5327077164133937 0.0 37 0.0 0.0 0.0 0.5657986656145442 0.0 38 0.0 0.0 0.0 0.6008694151952507 0.0 39 0.0 0.0 0.0 0.6377785508426879 0.0 40 0.0 0.0 0.0 0.674687686490125 0.0 41 0.0 0.0 0.0 0.7159806658351505 0.0 42 0.0 0.0 0.0 0.7691524474575121 0.0 43 0.0 0.0 0.0 0.8217585718285719 0.0 44 0.0 0.0 0.0 0.8793141971485218 0.0 45 0.0 0.0 0.0 0.9380011369710751 0.0 46 0.0 0.0 0.0 1.000506263239915 0.0 47 0.0 0.0 0.0 1.0599002746265955 0.0 48 0.0 0.0 0.0 1.1245266155878169 0.0 49 0.0 0.0 0.0 1.1932539716209758 0.0 50 0.0 0.0 0.0 1.2639611280336907 0.0 51 0.0 0.0 0.0 1.343435971841582 0.0 52 0.0 0.0 0.0 1.4265875877829346 0.0 53 0.0 0.0 0.0 1.5228907348170524 0.0 54 0.0 0.0 0.0 1.6196181247896462 0.0 55 0.0 0.0 0.0 1.726527345285671 0.0 56 0.0 0.0 0.0 1.8378204094792843 0.0 57 0.0 0.0 0.0 1.947699330544643 0.0 58 0.0 0.0 0.0 2.066770181943655 0.0 59 0.0 0.0 0.0 2.180325875142475 0.0 60 0.0 0.0 0.0 2.310002800003394 0.0 61 0.0 0.0 0.0 2.455518127900761 0.0 62 0.0 0.0 0.0 2.595376883285111 0.0 63 0.0 0.0 0.0 2.748104341136575 1.4141431282542968E-4 64 0.0 0.0 0.0 2.900690384675214 1.4141431282542968E-4 65 0.0 0.0 0.0 3.0681249310605225 1.4141431282542968E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCTATT 40 0.0022567427 35.498302 17 CTCGCAT 40 0.0022583099 35.493282 32 ACGGTAG 55 2.7564284E-4 32.26662 36 ACGCATT 50 0.0067301015 28.396631 7 AATTCGC 95 0.0066405875 18.684639 55 GACGCCT 100 0.008936786 17.746641 33 TAAGGCG 395 0.0 17.075134 17 ACATAAT 385 0.0 16.601307 49 AGTGTAC 280 3.2341632E-9 16.481354 9 TACATAA 390 0.0 16.38731 48 GTACATA 425 0.0 15.8731985 47 AATTTAC 385 3.6379788E-12 15.676794 53 TATTAAG 445 0.0 15.157651 14 CGATATG 165 5.8562524E-4 15.058821 40 AACCCGA 195 1.2545493E-4 14.561347 36 TTACACA 445 3.6379788E-12 14.358864 56 TCGTTCG 150 0.0053989585 14.200326 22 AGTATTA 460 5.456968E-12 13.893588 12 TAATTTA 435 3.092282E-11 13.874863 52 GTATTAA 475 9.094947E-12 13.452939 13 >>END_MODULE