FastQCFastQC Report
Thu 26 May 2016
SRR936146_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936146_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences707142
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19380.27406093825568273No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17300.2446467611879934No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10120.14311128457933484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCAG2000.041.66124100-101
GGTATCA23800.039.005011
AACGTAC554.6937748E-737.87117498-99
AGGTATC4950.037.27268100-101
CGTACAT3900.035.86224100-101
CCGTACA1500.035.709637100-101
AACGTAT609.275991E-734.71524498-99
AGTATCA5450.034.399193100-101
CGTATCA2750.033.545414100-101
GCCGTAC951.0750227E-931.32202398-99
TGTATCA6000.030.749964100-101
GGGTATC3350.029.313879100-101
GTATCAA39950.029.1952361
CTACGCA953.709465E-828.191816100-101
CGCCTAT557.7399134E-427.052752100-101
GTGGTAT7150.026.632791
CGGTATC3800.026.625605100-101
GCGTACA2150.026.297794100-101
TCGTATC1256.9849193E-1026.187065100-101
CAACGTA701.05466075E-425.50507798-99
Produced by FastQC (version 0.11.5)