FastQCFastQC Report
Thu 26 May 2016
SRR936145_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936145_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500943
Sequences flagged as poor quality0
Sequence length77
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148902.9723940647937988No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA98281.9618998568699433No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAC458.4672814E-539.4366264
TCCGCGA501.5714324E-435.50359751
CGAACTC707.774306E-735.50004625
CGCGAAT552.7505943E-432.27599353
AAGCGCG604.5673427E-429.60110771
TTCCGCG604.580721E-429.58632950
CCGGATT500.00672431328.40004243
GGGCGTA851.1891188E-425.05385634
TCGAACT1001.2503493E-524.85251824
AGTCACG1001.25325605E-524.8450744
GCCTCGA1201.9120016E-623.66906421
GAAGCGA750.001687464723.66433758
GATCGGG750.001688445523.66197411
CCTCGAA1252.726245E-622.722322
CGAATTG952.5258271E-422.41884655
TGATCGG800.002457192422.18310210
CGTAGGC800.002457192422.18310237
GCCTAGT850.003488920620.88238317
ATCGGGA850.003492961220.87821412
CTCGAAC1205.1147945E-520.7104323
Produced by FastQC (version 0.11.5)