FastQCFastQC Report
Thu 26 May 2016
SRR936145_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936145_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500943
Sequences flagged as poor quality0
Sequence length125
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86611.728939220629892No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66101.319511401496777No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53121.0604000854388622No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21490.42899092311899756No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18090.36111892969858844No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13350.2664973859301358No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11870.2369531064412518No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11820.23595498889095165No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9990.1994238865499668No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9440.18844459349666529No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5110.10200761364067368No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT5060.10100949609037355RNA PCR Primer, Index 26 (100% over 22bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA4400.056.13269100-101
CCGTACA1300.050.35815100-101
CGATATA302.1900949E-549.59515100-101
AGTATCA11400.049.33412100-101
TACGCAG3700.049.058987100-101
CCCGGTA800.048.3504498-99
AGGTATC8800.048.01712100-101
GGTATCA44000.047.87231
CGGTATC4250.046.91118100-101
CACGGTA800.044.63117698-99
TTTACGC1600.044.63117698-99
GCGTATC950.043.85255100-101
TACGGTA559.616997E-943.27871798-99
AAGTATC9750.042.72813100-101
CGACCGT355.4470194E-542.50588296-97
ACGTATC1900.042.28639100-101
CGTACAT3950.042.187267100-101
CACGTAT950.040.71616498-99
ACGGTAT1550.040.316055100-101
ACCGGTA300.001307541439.67215798-99
Produced by FastQC (version 0.11.5)