FastQCFastQC Report
Thu 26 May 2016
SRR936144_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936144_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences508842
Sequences flagged as poor quality0
Sequence length77
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152482.996607984403803No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA99491.955223821932938No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCGC305.482868E-447.3273736
CGGTTAC1200.044.3737684
GCCTAGT1550.034.35388617
GGGCGGT1750.030.4247381
CTAGTGG1800.029.5854219
TCGCGCT500.006728595628.39642337
CCTAGTG1950.027.30962218
TTGCGTA1304.64388E-927.30425336
GCGGTTA2000.026.6242643
GCGTAAG1356.9449015E-926.29298438
GGCGGTT2150.024.7643222
GTTACAA2250.023.6660126
GGGTCAT1952.5465852E-1123.66368533
TAGTGGC2450.023.18531220
GGTTACA2350.022.6589475
CGTAAGT1604.2202373E-822.18470639
GATATTC1806.160917E-921.6917111
TGCGTAA1655.844595E-821.51244237
CTCCGCG1003.56647E-421.30359863
GTTATGT850.0034856120.8858852
Produced by FastQC (version 0.11.5)