##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936144_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 508842 Sequences flagged as poor quality 0 Sequence length 77 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.30608715475531 40.0 38.0 41.0 34.0 41.0 2 38.17687022690737 40.0 38.0 41.0 34.0 41.0 3 38.050477358394154 40.0 38.0 41.0 34.0 41.0 4 37.94777553739668 40.0 38.0 41.0 34.0 41.0 5 37.83358685014209 40.0 38.0 41.0 34.0 41.0 6 37.6782753782117 40.0 37.0 41.0 34.0 41.0 7 37.43624543571482 40.0 37.0 41.0 33.0 41.0 8 37.23054504148636 40.0 36.0 41.0 33.0 41.0 9 37.28679825957763 40.0 36.0 41.0 33.0 41.0 10-11 37.398517614505096 40.0 36.0 41.0 33.0 41.0 12-13 37.267875882887026 40.0 36.0 41.0 33.0 41.0 14-15 37.06297141352326 40.0 36.0 41.0 33.0 41.0 16-17 36.81457014161567 40.0 35.0 41.0 32.5 41.0 18-19 36.504972073846105 40.0 35.0 41.0 31.5 41.0 20-21 36.07024479111394 40.0 35.0 41.0 30.5 41.0 22-23 35.49110136348808 40.0 35.0 41.0 27.0 41.0 24-25 35.04971779059119 39.0 35.0 41.0 23.5 41.0 26-27 34.05297027368024 38.0 34.0 40.0 21.5 40.5 28-29 34.042600650103566 38.5 34.5 40.0 20.0 40.5 30-31 34.37481477550988 39.0 35.0 40.5 20.0 41.0 32-33 34.219312674661296 38.5 35.0 41.0 19.0 41.0 34-35 33.9638031058757 38.0 35.0 40.0 16.5 41.0 36-37 33.649536594856556 37.5 34.0 40.0 11.5 41.0 38-39 33.33964570534665 37.0 34.0 40.0 7.5 41.0 40-41 32.98614992473105 36.5 34.0 40.0 2.0 41.0 42-43 32.62061799143939 36.0 33.0 39.0 2.0 41.0 44-45 32.25811057263355 35.5 33.0 39.0 2.0 41.0 46-47 31.837108572012532 35.0 33.0 38.5 2.0 40.0 48-49 31.436915584798424 35.0 33.0 37.5 2.0 40.0 50-51 31.00940960062259 35.0 32.0 37.0 2.0 39.0 52-53 30.564743083314664 35.0 32.0 36.5 2.0 39.0 54-55 30.140353980213895 35.0 31.0 36.0 2.0 38.5 56-57 29.73750692749419 35.0 31.0 35.5 2.0 37.0 58-59 29.322156976035785 35.0 31.0 35.0 2.0 37.0 60-61 28.974589361727215 34.0 30.0 35.0 2.0 36.5 62-63 28.622666760998502 34.0 30.0 35.0 2.0 36.0 64-65 28.267650862153676 34.0 29.0 35.0 2.0 36.0 66-67 27.921672149704627 34.0 29.0 35.0 2.0 35.0 68-69 27.58238510185873 34.0 28.5 35.0 2.0 35.0 70-71 27.177579680922562 34.0 27.0 35.0 2.0 35.0 72-73 26.765468455827154 34.0 26.5 35.0 2.0 35.0 74-75 26.295962204377783 34.0 25.0 35.0 2.0 35.0 76-77 24.788377335204245 31.5 20.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3124863562090354 1101 2 0.3313010123405107 1101 3 0.30857869773829094 1101 4 0.15264966166066785 1101 5 0.34438222384311956 1101 6 0.3653808727968837 1101 7 0.6071922672925041 1101 8 0.2806421093047149 1101 9 0.4403447606527209 1101 10-11 0.3962766874452157 1101 12-13 0.6514324919148251 1101 14-15 0.6092432015199023 1101 16-17 0.4696763613441206 1101 18-19 0.6972320873664657 1101 20-21 0.7742711158850213 1101 22-23 0.8043089388665194 1101 24-25 0.7021583154752733 1101 26-27 0.5610440320602734 1101 28-29 0.7046050249749314 1101 30-31 0.687807731343355 1101 32-33 0.6778474998605546 1101 34-35 0.6390753515921972 1101 36-37 0.6358039379237184 1101 38-39 0.8980299509839327 1101 40-41 0.902721416021329 1101 42-43 0.7787668257801101 1101 44-45 0.7043737124567642 1101 46-47 0.7351510115943789 1101 48-49 0.814533755805428 1101 50-51 0.6494496154352554 1101 52-53 0.7526505005960544 1101 54-55 0.6959028902281794 1101 56-57 0.8402496113965441 1101 58-59 1.0361178669615718 1101 60-61 0.9548246936507674 1101 62-63 0.946782911372015 1101 64-65 0.9246402147077326 1101 66-67 1.2495906243711516 1101 68-69 1.1731020822198666 1101 70-71 0.7980575350786552 1101 72-73 0.9501681449580204 1101 74-75 0.9886366629444616 1101 76-77 0.9118328722172961 1102 1 0.611538626469752 1102 2 0.7883439945973905 1102 3 0.8291612171093306 1102 4 0.5335082972327143 1102 5 0.46044441308620065 1102 6 0.7051817359755077 1102 7 0.8384395856337932 1102 8 0.6896486967204822 1102 9 0.673901460382659 1102 10-11 0.8347247326737559 1102 12-13 0.8242543540586311 1102 14-15 0.8763679585931783 1102 16-17 0.8376945840038204 1102 18-19 0.8843014445255548 1102 20-21 0.8541877005244771 1102 22-23 0.8441110640021847 1102 24-25 0.6928103686793889 1102 26-27 0.5423228993276297 1102 28-29 0.8189257134986931 1102 30-31 0.8779467990094929 1102 32-33 0.8753942553690024 1102 34-35 0.854292660594993 1102 36-37 0.9245586387137052 1102 38-39 0.9864268869522306 1102 40-41 1.0343687747452393 1102 42-43 1.2156184953492826 1102 44-45 0.9447986133931394 1102 46-47 0.8595042586098494 1102 48-49 0.7758737017936639 1102 50-51 0.6584789180777939 1102 52-53 0.7266100170141137 1102 54-55 0.9813765027064072 1102 56-57 1.07218469632625 1102 58-59 1.2129748367503197 1102 60-61 1.1809137625788608 1102 62-63 1.1671206110767152 1102 64-65 1.141810402234551 1102 66-67 1.1535146797963058 1102 68-69 1.2437022689895088 1102 70-71 1.1337128693710774 1102 72-73 1.3159905623629342 1102 74-75 0.9401522733530818 1102 76-77 0.7055659935418461 1103 1 0.0893908725516468 1103 2 0.16254615794067462 1103 3 0.23673994657641373 1103 4 0.07033177327684825 1103 5 -0.1425220378424541 1103 6 0.14076381332173327 1103 7 0.039622204774204306 1103 8 0.04907291262035329 1103 9 0.047895978550016594 1103 10-11 0.27316592436539366 1103 12-13 0.43094883453040467 1103 14-15 0.38111824131006955 1103 16-17 0.4286383792465145 1103 18-19 0.4530048441562613 1103 20-21 0.5748921607428628 1103 22-23 0.7213480414551086 1103 24-25 0.6660049737382607 1103 26-27 0.8391868182629594 1103 28-29 0.582286881934067 1103 30-31 0.8089990169794277 1103 32-33 0.7206203330474636 1103 34-35 0.778810444605682 1103 36-37 0.7605445060635745 1103 38-39 0.654990046225663 1103 40-41 0.5727400059852954 1103 42-43 0.4650993824094769 1103 44-45 0.5637377701620565 1103 46-47 0.48219380671005396 1103 48-49 0.521815037239655 1103 50-51 0.6140109050185103 1103 52-53 0.5197570873004089 1103 54-55 0.7363456114933165 1103 56-57 0.6184656114984044 1103 58-59 0.5323075609911392 1103 60-61 0.41796149541299243 1103 62-63 0.4852106588031617 1103 64-65 0.44918915342451626 1103 66-67 0.5350818578025311 1103 68-69 0.7720618082088926 1103 70-71 0.8254077012423728 1103 72-73 0.7631352814669299 1103 74-75 0.6114036390194322 1103 76-77 0.6340466058714966 1104 1 -0.15382570052855726 1104 2 0.061797466241216625 1104 3 0.07276309490141131 1104 4 0.1739262574053484 1104 5 0.13870846497787426 1104 6 0.30155108556284205 1104 7 0.45293694814357366 1104 8 0.2788690596593284 1104 9 0.28254334221300326 1104 10-11 0.3519504463104681 1104 12-13 0.5193402933332649 1104 14-15 0.559597811448981 1104 16-17 0.616839481911498 1104 18-19 0.3665583285012204 1104 20-21 0.3983845910623245 1104 22-23 0.5516493643984361 1104 24-25 0.652512925404352 1104 26-27 0.3137036065283567 1104 28-29 0.4936121171735053 1104 30-31 0.7090843270880356 1104 32-33 0.63706735801658 1104 34-35 0.6461675501737574 1104 36-37 0.8334989733847067 1104 38-39 0.7677108020477661 1104 40-41 0.8229341819787734 1104 42-43 0.7273483860638024 1104 44-45 0.650295698272366 1104 46-47 0.6890517208142377 1104 48-49 0.49627134445790944 1104 50-51 0.5297687643714255 1104 52-53 0.17286326655349882 1104 54-55 0.41576104625654864 1104 56-57 0.3615262071412211 1104 58-59 0.43860013646511575 1104 60-61 0.35819348797700457 1104 62-63 0.37408787591101955 1104 64-65 0.10549127853752083 1104 66-67 0.1396615463569688 1104 68-69 0.22274747229078784 1104 70-71 0.38759654217085426 1104 72-73 0.451941194603414 1104 74-75 0.6969699962777902 1104 76-77 0.28860592186268974 1105 1 -0.19522756107935635 1105 2 0.0469354285828274 1105 3 -0.06230776129406479 1105 4 -0.27122433668141355 1105 5 -0.37762727628187776 1105 6 -0.1789585264057365 1105 7 0.09608356849383881 1105 8 -0.13165607882933728 1105 9 -0.21691717700435476 1105 10-11 -0.04510083068002757 1105 12-13 -0.15501726400302118 1105 14-15 -0.006616875586374249 1105 16-17 -0.10624569026182229 1105 18-19 -0.01675263611673472 1105 20-21 -0.15399603236692627 1105 22-23 -0.3457654042169409 1105 24-25 -0.4173580597401809 1105 26-27 -0.4672787090284274 1105 28-29 -0.09483312552705314 1105 30-31 0.024963483392724584 1105 32-33 -0.07258671637913494 1105 34-35 0.1500245088179497 1105 36-37 0.30927661519578464 1105 38-39 0.1619232258954426 1105 40-41 0.11435906914834248 1105 42-43 0.4269727232261289 1105 44-45 0.03876133597812981 1105 46-47 0.16758433308545406 1105 48-49 0.02773063997495484 1105 50-51 0.25043449681527363 1105 52-53 0.36223417932866653 1105 54-55 0.04924635527376964 1105 56-57 0.1474740485601913 1105 58-59 0.36806631365165643 1105 60-61 0.6478222988824136 1105 62-63 0.5560080327551908 1105 64-65 0.3729158499134577 1105 66-67 0.4630720517583562 1105 68-69 0.17999690978338734 1105 70-71 0.013231418945547802 1105 72-73 -0.4317381959148854 1105 74-75 -0.007379732008104156 1105 76-77 -0.09285139994418046 1106 1 -0.221201587053379 1106 2 -0.4627676908569214 1106 3 -0.35872190846639285 1106 4 -0.006429541160365204 1106 5 0.1787655195362916 1106 6 -0.10655739734060177 1106 7 -0.06790381416872293 1106 8 -0.11110280927367455 1106 9 0.09582833765656318 1106 10-11 0.0191916206834577 1106 12-13 0.3180050581813134 1106 14-15 0.2576147693316244 1106 16-17 0.04305486286953908 1106 18-19 0.27413192491946603 1106 20-21 0.2790702362939186 1106 22-23 0.4094834703455845 1106 24-25 0.19624980087485255 1106 26-27 0.19551845656203426 1106 28-29 0.34306138219985627 1106 30-31 0.7010015979783262 1106 32-33 0.7496114366250879 1106 34-35 0.7607768849334491 1106 36-37 0.8840011723624883 1106 38-39 0.5440589140965741 1106 40-41 0.6421227393701159 1106 42-43 0.556967229916566 1106 44-45 0.272071521403241 1106 46-47 0.6540859931705079 1106 48-49 0.6500759722165341 1106 50-51 0.45299769578709004 1106 52-53 0.3493559960594226 1106 54-55 0.3260486963219229 1106 56-57 0.42300843702355095 1106 58-59 0.4864229503642399 1106 60-61 0.3991222094002005 1106 62-63 0.6287235587827666 1106 64-65 0.5664841863389363 1106 66-67 0.3083835638695476 1106 68-69 0.2781181288144232 1106 70-71 0.08691604769801486 1106 72-73 0.28209492494137933 1106 74-75 0.3981318885873115 1106 76-77 0.3244086478026986 1107 1 0.0791647133129203 1107 2 0.00478710072021471 1107 3 0.21353544312056272 1107 4 0.0965745311174544 1107 5 -0.08411126848015726 1107 6 -0.11866168039460234 1107 7 0.06991925661524334 1107 8 -0.15387195818807697 1107 9 -0.04439921877211361 1107 10-11 0.2097752456246269 1107 12-13 0.25607164708588925 1107 14-15 -0.003179836435045047 1107 16-17 0.101427588840032 1107 18-19 0.10876470368631885 1107 20-21 -0.020201255715008415 1107 22-23 0.044820200654378084 1107 24-25 0.3710559072452071 1107 26-27 0.2978728061230882 1107 28-29 0.27920949851557 1107 30-31 0.4666051750344167 1107 32-33 0.5014582862372947 1107 34-35 0.37912873792217994 1107 36-37 0.4398722100770982 1107 38-39 0.4440220794228722 1107 40-41 0.45285390764318123 1107 42-43 0.40165280504906775 1107 44-45 0.5204344247940753 1107 46-47 0.5885918969501596 1107 48-49 0.4719649770664347 1107 50-51 0.5755855362520279 1107 52-53 0.4409055079999966 1107 54-55 0.43294598897340464 1107 56-57 0.33041016006773205 1107 58-59 0.3823496493635652 1107 60-61 0.24247764353775736 1107 62-63 0.3240138364752774 1107 64-65 0.3897442595990128 1107 66-67 0.4767845297565465 1107 68-69 0.2721785376793022 1107 70-71 0.1684690384688814 1107 72-73 0.1961933963024194 1107 74-75 0.21897724434887067 1107 76-77 -0.13999473280179586 1108 1 0.036625300607759925 1108 2 0.003512132594416073 1108 3 0.23483550585709168 1108 4 0.11664010090677834 1108 5 0.14803411836135894 1108 6 0.26715784078403715 1108 7 0.42964240639363993 1108 8 0.24021461821894263 1108 9 0.33024915311383296 1108 10-11 0.28537781984598354 1108 12-13 0.4885813105347765 1108 14-15 0.49101703483364645 1108 16-17 0.7798522948890465 1108 18-19 0.6703375691265876 1108 20-21 0.7951509795267313 1108 22-23 0.7322506339411703 1108 24-25 0.7704599102403762 1108 26-27 0.742032237851582 1108 28-29 0.8024068352488172 1108 30-31 1.0635179307869507 1108 32-33 0.7790876550268848 1108 34-35 0.5433061811051516 1108 36-37 0.6393794247776796 1108 38-39 0.5801299862490126 1108 40-41 0.5051174820062059 1108 42-43 0.34849371735960233 1108 44-45 0.2540925714356419 1108 46-47 0.31582966838682225 1108 48-49 0.27469401598332155 1108 50-51 0.1379982616481783 1108 52-53 0.4134886338980408 1108 54-55 0.39586566598147854 1108 56-57 0.5279087940304521 1108 58-59 0.4378360598223523 1108 60-61 0.5411996397735663 1108 62-63 0.6221287050321358 1108 64-65 0.6981600611087373 1108 66-67 0.7957339964960717 1108 68-69 0.9573777767459468 1108 70-71 0.8444702931994179 1108 72-73 0.9169601985609361 1108 74-75 0.826838535911822 1108 76-77 1.0284427229284745 1109 1 -0.14090961625046106 1109 2 -0.3642003595081178 1109 3 -0.32182457399127884 1109 4 -0.09125467131028842 1109 5 -0.20522434032315573 1109 6 -0.3887346730953354 1109 7 -0.14885163186367834 1109 8 -0.3564133340871152 1109 9 -0.4701097612709759 1109 10-11 -0.7328065035264686 1109 12-13 -0.815707850788499 1109 14-15 -0.7329230251198382 1109 16-17 -0.6377872717251947 1109 18-19 -0.5088558144816417 1109 20-21 -0.8647709196277376 1109 22-23 -0.928445888487623 1109 24-25 -0.9584773940034239 1109 26-27 -1.1874417576586325 1109 28-29 -1.153997501297269 1109 30-31 -1.0149929105782718 1109 32-33 -1.1028637907125187 1109 34-35 -1.0952001570271577 1109 36-37 -1.0982450862518789 1109 38-39 -1.190596647349146 1109 40-41 -1.2963907649591562 1109 42-43 -1.1079391844699273 1109 44-45 -0.9252789251163556 1109 46-47 -0.9809216187312408 1109 48-49 -0.8676115048458115 1109 50-51 -0.9279304125193946 1109 52-53 -1.0184772625141605 1109 54-55 -0.894444989877428 1109 56-57 -0.902852319545115 1109 58-59 -1.0256541473263603 1109 60-61 -1.2012036751435602 1109 62-63 -0.9589866180580238 1109 64-65 -0.8821167868970718 1109 66-67 -0.8178499719947432 1109 68-69 -1.2139080642832134 1109 70-71 -1.1168943207995845 1109 72-73 -1.0658216567710248 1109 74-75 -0.938344752389181 1109 76-77 -1.006736263776908 1110 1 -0.6260980088989498 1110 2 -0.587419186401327 1110 3 -0.7434708274450301 1110 4 -0.9288905875680058 1110 5 -0.7483773225767223 1110 6 -0.6751588555358481 1110 7 -0.42415164423532303 1110 8 -0.7340130976512498 1110 9 -0.9951142288091575 1110 10-11 -0.5976428541663523 1110 12-13 -0.4684787554306169 1110 14-15 -0.31816193531545167 1110 16-17 -0.28101482750069096 1110 18-19 -0.4248128298912164 1110 20-21 -0.4454718420025472 1110 22-23 -0.2341371884725021 1110 24-25 -0.3532720247098382 1110 26-27 -0.29554930848546235 1110 28-29 -0.23683425057902951 1110 30-31 -0.29494259922522303 1110 32-33 -0.2428661275786368 1110 34-35 -0.1948039207562431 1110 36-37 -0.1031370405592753 1110 38-39 -0.14819455516664704 1110 40-41 0.0515273622488408 1110 42-43 -0.1394525114799734 1110 44-45 -0.03534360132293557 1110 46-47 -0.14690612668345793 1110 48-49 -0.13460876933782018 1110 50-51 -0.14861305235334044 1110 52-53 -0.21810920609946294 1110 54-55 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-0.28043315964740856 2314 6 -0.17742456699254205 2314 7 -0.4062938773298512 2314 8 -0.31721926380342325 2314 9 -0.38908786346075885 2314 10-11 -0.5849436094035099 2314 12-13 -0.3037788117336433 2314 14-15 -0.24250425771823103 2314 16-17 -0.6687019379905266 2314 18-19 -0.5269892330985471 2314 20-21 -0.27249535336993347 2314 22-23 0.10238600255008379 2314 24-25 0.0675911589351017 2314 26-27 0.19642459797384504 2314 28-29 0.2856461889728834 2314 30-31 0.15872971715896256 2314 32-33 0.06875559374078932 2314 34-35 -0.12358641633936429 2314 36-37 -0.7279783242682853 2314 38-39 -0.029653386005172422 2314 40-41 0.13094763522144603 2314 42-43 0.34303530570991825 2314 44-45 0.3836363431506342 2314 46-47 0.35477519818500625 2314 48-49 0.5367718197083278 2314 50-51 0.6647928924696913 2314 52-53 0.7638408201105662 2314 54-55 0.29843634054622825 2314 56-57 0.25463507604522206 2314 58-59 0.3929176037459534 2314 60-61 0.4882293145471621 2314 62-63 0.3865284696084679 2314 64-65 0.37507525163103494 2314 66-67 0.5479619777381188 2314 68-69 0.4277485690225298 2314 70-71 0.3036508669496776 2314 72-73 0.7762338026314879 2314 74-75 1.0637810659568139 2314 76-77 0.9047845429053112 2315 1 -0.9949084836051014 2315 2 -0.8046735535680227 2315 3 -0.6259713144896395 2315 4 -0.7242303823598775 2315 5 -0.8251274362204626 2315 6 -0.7307306459118408 2315 7 -1.0457179797116467 2315 8 -0.7881399890078313 2315 9 -0.7472775186331688 2315 10-11 -0.7494343666022303 2315 12-13 -0.6809073222206621 2315 14-15 -0.4010893981377137 2315 16-17 -0.7074528267214149 2315 18-19 -1.057834860542556 2315 20-21 -0.9344443402167144 2315 22-23 -1.1872002932195613 2315 24-25 -1.0277496693620947 2315 26-27 -1.1265935321363543 2315 28-29 -1.1673532777885853 2315 30-31 -1.3447185587031072 2315 32-33 -1.5461528669773017 2315 34-35 -1.6311695126778005 2315 36-37 -1.5559911219931344 2315 38-39 -1.4662935388668856 2315 40-41 -1.5222984543626268 2315 42-43 -1.525043702466398 2315 44-45 -1.3808855196293166 2315 46-47 -1.216732089408314 2315 48-49 -1.426516928958577 2315 50-51 -1.1431723581524196 2315 52-53 -1.4213529232239495 2315 54-55 -1.7053718713272126 2315 56-57 -1.5059230469551323 2315 58-59 -1.4268544367801752 2315 60-61 -1.4695226406608022 2315 62-63 -1.2730676022009852 2315 64-65 -1.3006135148104896 2315 66-67 -1.1787155018210704 2315 68-69 -1.1483090207392905 2315 70-71 -1.123462090746731 2315 72-73 -0.9105863182359144 2315 74-75 -1.0984567584299576 2315 76-77 -0.8480793923950785 2316 1 -1.4120618021071394 2316 2 -0.9203576470051757 2316 3 -1.2546791379972433 2316 4 -1.5863688695678846 2316 5 -1.5809209104510913 2316 6 -1.8095218954483627 2316 7 -1.3706160145651936 2316 8 -0.9889186319444221 2316 9 -1.5815609817403242 2316 10-11 -1.5065487529574426 2316 12-13 -1.7838225984535185 2316 14-15 -2.882921062950466 2316 16-17 -2.0227452813005797 2316 18-19 -1.6985583496044754 2316 20-21 -1.441697769953457 2316 22-23 -2.011808654361566 2316 24-25 -1.9627353015460045 2316 26-27 -2.0775112184544824 2316 28-29 -2.235368665256143 2316 30-31 -2.300410067787279 2316 32-33 -2.701070367904258 2316 34-35 -2.7536551448617033 2316 36-37 -2.552654080836291 2316 38-39 -2.760057443205042 2316 40-41 -2.076745525182055 2316 42-43 -1.7614985597151964 2316 44-45 -2.0015288270078884 2316 46-47 -1.9487027976026035 2316 48-49 -1.8152706367820812 2316 50-51 -1.6095586206663235 2316 52-53 -2.167876838685576 2316 54-55 -2.0987951193806076 2316 56-57 -2.2421114043893198 2316 58-59 -2.1109594608810305 2316 60-61 -1.863838083745712 2316 62-63 -1.9277881250041027 2316 64-65 -1.850182480327728 2316 66-67 -1.9842517457958557 2316 68-69 -2.4504966366087757 2316 70-71 -2.2684032290485447 2316 72-73 -2.398350500660829 2316 74-75 -2.9074111522008153 2316 76-77 -3.154055940411393 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7281.0 3 843.0 4 1440.0 5 2020.0 6 2111.0 7 2373.0 8 3114.0 9 3596.0 10 6104.0 11 7767.0 12 4740.0 13 2792.0 14 2428.0 15 2261.0 16 2153.0 17 2203.0 18 2245.0 19 2238.0 20 2446.0 21 2332.0 22 2790.0 23 3058.0 24 3463.0 25 3884.0 26 4584.0 27 5237.0 28 6266.0 29 7287.0 30 8990.0 31 11307.0 32 14849.0 33 20586.0 34 34063.0 35 49081.0 36 68218.0 37 95564.0 38 86194.0 39 20814.0 40 120.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 20.038362216361328 26.559742470039343 33.34171851723745 20.06017679636188 2 19.92963974764647 26.99042864723571 33.1728936145123 19.90703799060553 3 19.709545607314624 26.959898897185276 32.95020136048539 20.380354135014713 4 19.759213759213758 26.97002457002457 33.244619164619166 20.026142506142506 5 19.816660901286205 27.06217176640775 33.003985974401715 20.117181357904336 6 19.791816125209078 27.17062940636541 33.193456516654514 19.844097951770998 7 19.671512473562967 27.044201935701988 32.994795147379094 20.28949044335595 8 19.45226858613119 27.457135387899434 32.51833404422185 20.572261981747527 9 19.513561430854296 27.201091282906052 32.93877847861168 20.346568807627975 10-11 19.892497953254594 27.52722687374259 32.435091388533706 20.145183784469108 12-13 20.000471750962667 27.24863044798651 32.20162243018577 20.549275370865054 14-15 19.694306022194947 27.462439282285413 32.23396968806597 20.609285007453675 16-17 19.86942388497353 27.551285125250086 32.22757663780261 20.351714351973772 18-19 19.855599570356148 27.78661677085104 31.636901807904383 20.720881850888425 20-21 19.76611520300316 28.12395894240833 31.487772640392297 20.622153214196217 22-23 20.057372199935923 28.354819245139872 30.548288759648024 21.039519795276178 24-25 20.139498360201394 28.698588603669968 29.764236567361475 21.39767646876717 26-27 20.282476048401442 28.906329079093098 29.510610049598405 21.300584822907055 28-29 20.364792717117115 29.06185910443889 29.257511897871403 21.315836280572594 30-31 20.13830269166479 29.095057796453105 29.206592225215033 21.560047286667068 32-33 20.3713621690145 29.329356675723883 28.93755435557019 21.361726799691432 34-35 20.33648294737049 28.90962425743304 29.195988541497762 21.55790425369871 36-37 20.436340837599094 29.025677108566338 29.46838587695153 21.069596176883035 38-39 20.487180217959388 29.049294909530833 29.48535127745745 20.978173595052326 40-41 20.316928398749976 29.46736374535663 29.27553607578765 20.94017178010574 42-43 20.301605318631424 29.57176761944072 29.096903852862987 21.029723209064866 44-45 20.40276001961615 29.3101228564493 29.32515928292953 20.96195784100502 46-47 20.264833236040435 29.51178619934627 29.17725586853076 21.046124696082536 48-49 20.35354746396324 29.631318471813294 28.878349640123744 21.13678442409972 50-51 19.867238546032095 29.927840198768703 28.944316457388176 21.260604797811027 52-53 19.723498318305214 29.80069312166192 29.16723181833372 21.308576741699152 54-55 19.690211996422498 29.840978112376774 29.212458352580423 21.2563515386203 56-57 19.62787196342598 30.113615703555492 28.88822498680658 21.370287346211942 58-59 19.484500317924557 29.958812308545046 28.799640701026107 21.757046672504295 60-61 19.60483887611805 29.652808967783084 28.7247582085269 22.01759394757196 62-63 19.573998832388824 29.587149200658107 28.76018956973332 22.078662397219748 64-65 19.61256057733604 29.40174014570993 28.80655645547277 22.179142821481257 66-67 19.39656189068495 29.58774922951129 28.94374253097679 22.07194634882697 68-69 19.477509619779152 29.28363974111368 29.069866871102327 22.168983768004836 70-71 19.2914624339623 29.190383220182024 29.091289806944797 22.426864538910877 72-73 19.551469865303314 28.914364369285224 29.12987906793826 22.404286697473207 74-75 19.414713959687592 28.909732184734228 29.050734164071635 22.62481969150655 76-77 19.149402684233813 28.63507202202448 29.77375743572096 22.441767858020746 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 20369.0 1 11558.0 2 2438.5 3 2059.0 4 1988.0 5 1923.5 6 1824.0 7 1843.0 8 1821.0 9 1745.0 10 1752.5 11 1732.5 12 1756.5 13 1808.0 14 1897.0 15 2013.0 16 2160.5 17 2281.0 18 2432.5 19 2709.5 20 3033.5 21 3477.0 22 3722.0 23 4029.0 24 4677.5 25 5410.0 26 5801.0 27 6312.5 28 7467.0 29 8506.5 30 8903.0 31 9856.0 32 11600.0 33 12951.5 34 14074.0 35 14636.0 36 15032.0 37 16221.5 38 16883.0 39 16751.0 40 17243.0 41 18272.0 42 19375.5 43 19942.0 44 19659.5 45 19332.0 46 19537.0 47 19761.0 48 19735.0 49 19693.5 50 19576.5 51 18989.0 52 18477.0 53 18125.0 54 16893.0 55 15204.5 56 13623.5 57 12851.0 58 12624.0 59 11361.0 60 9858.0 61 9391.0 62 8361.5 63 5952.5 64 3888.5 65 3204.0 66 2817.5 67 2262.0 68 1804.5 69 1321.0 70 1126.0 71 948.0 72 660.5 73 543.0 74 535.0 75 483.5 76 331.0 77 194.0 78 158.0 79 132.5 80 100.0 81 76.0 82 51.0 83 43.0 84 35.0 85 22.0 86 17.0 87 17.0 88 12.0 89 8.5 90 8.0 91 6.0 92 9.5 93 9.5 94 5.0 95 5.0 96 6.0 97 4.5 98 2.5 99 6.5 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0015721972635906628 2 0.006288789054362651 3 0.01041580687128814 4 0.01808026853129262 5 0.011398430161032305 6 0.011988004134878804 7 0.016901120583599626 8 0.01749069455744612 9 0.016311546609753126 10-11 0.02112640072949953 12-13 0.01945594113693445 14-15 0.006387051383337068 16-17 0.003930493158976657 18-19 0.009531445910518393 20-21 0.00874534727872306 22-23 0.011594954818981137 24-25 0.01778548154436937 26-27 0.010907118516160223 28-29 0.006387051383337068 30-31 0.005994002067439402 32-33 0.007762723988978898 34-35 0.009727970568467225 36-37 0.008352297962825396 38-39 0.015230660991034546 40-41 0.010219282213339308 42-43 0.012675840437699717 44-45 0.015230660991034546 46-47 0.013560201398469467 48-49 0.01100538084513464 50-51 0.021617712374371614 52-53 0.025351680875399435 54-55 0.021028138400525115 56-57 0.012872365095648552 58-59 0.014444562359239216 60-61 0.017195907570522875 62-63 0.021814237032320445 64-65 0.017982006202318206 66-67 0.014542824688213631 68-69 0.02446731991462969 70-71 0.04539719598618039 72-73 0.05738520012105919 74-75 0.06652359671567991 76-77 0.062298316569780014 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 508842.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.7589733664629 #Duplication Level Percentage of deduplicated Percentage of total 1 80.55165226011641 48.94235694296994 2 11.186349466957575 13.593422186616433 3 3.641821271952667 6.638199650039704 4 1.6007995671092747 3.890517530521511 5 0.864628469230647 2.626696906693523 6 0.5216826610724008 1.9018141745906092 7 0.2946936744329792 1.253369958129693 8 0.22597264996619001 1.0983892976675826 9 0.1690466747216733 0.924399216639294 >10 0.8610145081957713 9.465188412735733 >50 0.054246736800081345 2.2455798520639485 >100 0.027446264974375908 2.476690504209192 >500 0.0 0.0 >1k 0.0 0.0 >5k 3.2289723499265673E-4 1.9518848088068124 >10k+ 3.2289723499265673E-4 2.9914905583160394 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15248 2.996607984403803 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9949 1.955223821932938 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 7.860986317953314E-4 0.0 3 0.0 0.0 0.0 7.860986317953314E-4 0.0 4 0.0 0.0 0.0 0.001179147947692997 0.0 5 0.0 0.0 0.0 0.00137567260564183 0.0 6 0.0 0.0 0.0 0.0029478698692324925 0.0 7 0.0 0.0 0.0 0.0060922643964138185 0.0 8 0.0 0.0 0.0 0.009236658923595143 0.0 9 0.0 0.0 0.0 0.01238105345077647 0.0 10 0.0 0.0 0.0 0.01729416989949729 0.0 11 0.0 0.0 0.0 0.023779483611808774 0.0 12 0.0 0.0 0.0 0.03144394527181325 0.0 13 0.0 0.0 0.0 0.0406806041954084 0.0 14 0.0 0.0 0.0 0.05168598504054304 0.0 15 0.0 0.0 0.0 0.0676044823343985 0.0 16 0.0 0.0 0.0 0.08391602894415162 0.0 17 0.0 0.0 0.0 0.09767275500056992 0.0 18 0.0 0.0 0.0 0.11044685776724406 0.0 19 0.0 0.0 0.0 0.12479315779750885 0.0 20 0.0 0.0 0.0 0.14228385235495497 0.0 21 0.0 0.0 0.0 0.1591849729385546 0.0 22 0.0 0.0 0.0 0.17569304420625656 0.0 23 0.0 0.0 0.0 0.1937733127375492 0.0 24 0.0 0.0 0.0 0.22050066621859044 0.0 25 0.0 0.0 0.0 0.24192185393501323 0.0 26 0.0 0.0 0.0 0.26766658412631034 0.0 27 0.0 0.0 0.0 0.2943939376073516 0.0 28 0.0 0.0 0.0 0.31856647053505804 0.0 29 0.0 0.0 0.0 0.33959460893558313 0.0 30 0.0 0.0 0.0 0.36651848707457324 0.0 31 0.0 0.0 0.0 0.39521108713510283 0.0 32 0.0 0.0 0.0 0.42449326116947894 0.0 33 0.0 0.0 0.0 0.4586885516525758 0.0 34 0.0 0.0 0.0 0.4981900079002913 0.0 35 0.0 0.0 0.0 0.5406393340172392 0.0 36 0.0 0.0 0.0 0.5850539067136754 0.0 37 0.0 0.0 0.0 0.6316302506475487 0.0 38 0.0 0.0 0.0 0.6784031192393709 0.0 39 0.0 0.0 0.0 0.7212454946722165 0.0 40 0.0 0.0 0.0 0.7662496413424993 0.0 41 0.0 0.0 0.0 0.8138086085661168 0.0 42 0.0 0.0 0.0 0.865298068948711 0.0 43 0.0 0.0 0.0 0.9266137622287468 0.0 44 0.0 0.0 0.0 0.99343214593135 0.0 45 0.0 0.0 0.0 1.0641810227929298 0.0 46 0.0 0.0 0.0 1.1422013119986165 0.0 47 0.0 0.0 0.0 1.2231694710735357 0.0 48 0.0 0.0 0.0 1.3017793342530688 0.0 49 0.0 0.0 0.0 1.3772448029054205 0.0 50 0.0 0.0 0.0 1.4676461455618837 0.0 51 0.0 0.0 0.0 1.5627640800091187 0.0 52 0.0 0.0 0.0 1.6614194582994328 0.0 53 0.0 0.0 0.0 1.7726524146984721 0.0 54 0.0 0.0 0.0 1.8866367163087954 0.0 55 0.0 0.0 0.0 2.0098576768427137 0.0 56 0.0 0.0 0.0 2.1411361483525337 0.0 57 0.0 0.0 0.0 2.2732007184941496 0.0 58 0.0 0.0 0.0 2.412340176321923 0.0 59 0.0 0.0 0.0 2.5634676382845756 0.0 60 0.0 0.0 0.0 2.709878508456456 0.0 61 0.0 0.0 0.0 2.8682773827632153 0.0 62 0.0 0.0 0.0 3.0294276022812583 0.0 63 0.0 0.0 0.0 3.189595198509557 0.0 64 0.0 0.0 0.0 3.3794380180881296 0.0 65 0.0 0.0 0.0 3.5649572951918276 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCGCGC 30 5.482868E-4 47.32737 36 CGGTTAC 120 0.0 44.373768 4 GCCTAGT 155 0.0 34.353886 17 GGGCGGT 175 0.0 30.424738 1 CTAGTGG 180 0.0 29.58542 19 TCGCGCT 50 0.0067285956 28.396423 37 CCTAGTG 195 0.0 27.309622 18 TTGCGTA 130 4.64388E-9 27.304253 36 GCGGTTA 200 0.0 26.624264 3 GCGTAAG 135 6.9449015E-9 26.292984 38 GGCGGTT 215 0.0 24.764322 2 GTTACAA 225 0.0 23.666012 6 GGGTCAT 195 2.5465852E-11 23.663685 33 TAGTGGC 245 0.0 23.185312 20 GGTTACA 235 0.0 22.658947 5 CGTAAGT 160 4.2202373E-8 22.184706 39 GATATTC 180 6.160917E-9 21.691711 1 TGCGTAA 165 5.844595E-8 21.512442 37 CTCCGCG 100 3.56647E-4 21.303598 63 GTTATGT 85 0.00348561 20.88588 52 >>END_MODULE