FastQCFastQC Report
Thu 26 May 2016
SRR936144_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936144_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences508842
Sequences flagged as poor quality0
Sequence length125
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86411.6981695693358647No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66521.307282024675636No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54441.069880237873446No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22280.4378569379099996No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17650.34686602127968996No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13390.2631465169934872No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12300.2417253292770644No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11940.23465044159090642No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10020.1969177072647305No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9280.18237488257651688No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCGA300.004236180359.4965136
CGTATCA4400.054.10901100-101
GGTATCA42700.052.1270681
TACGCAG3550.050.29851100-101
ACGTATC1350.048.497704100-101
CGCGAGT255.327398E-447.60890698-99
CGGTATC3300.046.89447100-101
AGTATCA11550.046.63681100-101
GCGTATC900.046.293262100-101
CGTACAT4600.045.286884100-101
CCGTACA1850.045.04209100-101
ACGGTAT800.044.639927100-101
ACCGTAC604.1654857E-1044.6333598-99
AGGTATC7400.044.23777100-101
TTTACGC1950.042.7259498-99
CGACGCA704.0017767E-1142.51422100-101
GGCGTAC355.445516E-542.50795498-99
GTCTCGC355.4470947E-542.50586396-97
TCTACGC502.2219501E-741.6577998-99
TTCGGTA502.2219501E-741.6577998-99
Produced by FastQC (version 0.11.5)