FastQCFastQC Report
Thu 26 May 2016
SRR936143_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936143_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2165120
Sequences flagged as poor quality0
Sequence length77
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145780.6733114099911321No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA64320.297073603310671No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGC2300.023.16454368
GTTAGCG1604.2366082E-822.1844836
CAATTCG3500.021.30054754
TACGTAG4100.020.7752632
AATAGCG1205.130595E-520.7074339
CGCGGAA3200.019.9683459
ATTCGCG3700.019.18968456
TAACGCG1501.3235347E-518.93600852
AATTCGC3750.018.93381955
ATACGTA4350.018.7662621
TTAGTCG4200.018.59399815
TGTTTCG3250.018.56799142
CGGTTAC3650.018.4752314
CGATCGT3900.018.2013911
AACTCGA3950.017.97306813
ATTGCCG1200.001220819317.74799525
ACGCGAA2055.7541E-717.31751854
AACGCGA1653.0066763E-517.2133653
TAGTCGG4550.017.1636916
TCGCGCT4450.016.75169237
Produced by FastQC (version 0.11.5)