##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936143_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2165120 Sequences flagged as poor quality 0 Sequence length 77 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.7485903783624 40.0 36.0 41.0 30.0 41.0 2 36.603553151788354 39.0 36.0 41.0 30.0 41.0 3 36.508444797516994 39.0 36.0 41.0 30.0 41.0 4 36.382232393585575 39.0 36.0 41.0 30.0 41.0 5 36.29262257981082 39.0 36.0 41.0 30.0 41.0 6 36.1715147428318 39.0 35.0 40.0 29.0 41.0 7 35.9689897095773 39.0 35.0 40.0 29.0 41.0 8 35.76193282589418 38.0 35.0 40.0 28.0 41.0 9 35.62954339713272 38.0 35.0 40.0 28.0 41.0 10-11 35.80171075968076 39.0 35.0 40.0 28.5 41.0 12-13 35.91909432271652 39.0 35.0 41.0 29.0 41.0 14-15 35.82622210316288 39.0 35.0 41.0 28.0 41.0 16-17 35.60765592669229 39.0 35.0 41.0 27.0 41.0 18-19 35.38412790053208 39.0 35.0 41.0 26.5 41.0 20-21 35.08334387932309 39.0 35.0 41.0 25.5 41.0 22-23 34.6916097029264 39.0 34.0 41.0 23.5 41.0 24-25 34.36958713604788 39.0 34.0 41.0 22.5 41.0 26-27 33.38953198898906 37.5 33.0 39.5 19.0 40.5 28-29 33.40175325155188 38.0 33.0 39.5 19.0 40.5 30-31 33.742039240319244 38.0 33.0 40.0 18.5 41.0 32-33 33.569071460242384 38.0 33.0 40.0 17.5 41.0 34-35 33.28525693725983 37.5 33.0 40.0 13.5 41.0 36-37 32.99009015666568 37.0 32.5 40.0 12.0 41.0 38-39 32.69922452335206 36.5 32.0 40.0 8.5 41.0 40-41 32.3066802301951 36.0 32.0 39.5 7.5 41.0 42-43 31.940138421888854 35.5 31.0 39.0 4.0 41.0 44-45 31.57101176840083 35.0 31.0 39.0 2.0 40.5 46-47 31.182914803798404 35.0 31.0 38.0 2.0 40.0 48-49 30.732393354640852 35.0 30.5 37.0 2.0 39.5 50-51 30.30792311742536 35.0 30.0 37.0 2.0 39.0 52-53 29.870177172627848 35.0 30.0 36.0 2.0 39.0 54-55 29.428150633683124 34.0 29.0 36.0 2.0 37.5 56-57 28.995978975761158 34.0 29.0 35.0 2.0 37.0 58-59 28.581350918193912 34.0 29.0 35.0 2.0 37.0 60-61 28.244057373263374 34.0 28.5 35.0 2.0 36.0 62-63 27.92171888856045 34.0 27.5 35.0 2.0 36.0 64-65 27.561628454773867 34.0 27.0 35.0 2.0 35.5 66-67 27.187321949822643 34.0 26.0 35.0 2.0 35.0 68-69 26.842530437112032 33.0 25.5 35.0 2.0 35.0 70-71 26.426581436594738 33.0 25.0 35.0 2.0 35.0 72-73 25.952483234185635 33.0 23.5 35.0 2.0 35.0 74-75 25.38615411616908 33.0 19.5 35.0 2.0 35.0 76-77 23.730156296186816 31.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.8975309763471557 1101 2 0.8464939276969048 1101 3 0.8959060966568941 1101 4 0.8568164142856531 1101 5 0.8165715542582319 1101 6 0.8400248799375163 1101 7 0.7226593932211927 1101 8 0.7670110678234252 1101 9 0.9767307447360665 1101 10-11 0.8160813466718011 1101 12-13 0.7567582994258331 1101 14-15 0.9342799928575332 1101 16-17 0.8986644412263303 1101 18-19 0.860680157632153 1101 20-21 0.8695388693899915 1101 22-23 1.0625725318969472 1101 24-25 1.0531935568637465 1101 26-27 0.9886422918362285 1101 28-29 1.0435872864411024 1101 30-31 0.9485797322208427 1101 32-33 0.9628725763039938 1101 34-35 0.9690381223827202 1101 36-37 1.0346666974610628 1101 38-39 1.049029055508008 1101 40-41 0.9879193620363651 1101 42-43 0.7809640379567959 1101 44-45 0.7332926668551352 1101 46-47 0.8637825513491038 1101 48-49 0.913753405000282 1101 50-51 0.8967161750124042 1101 52-53 1.029652711694876 1101 54-55 0.8080031067263924 1101 56-57 0.9272806589885825 1101 58-59 1.0217658948052915 1101 60-61 1.0466148411684522 1101 62-63 0.890287961585905 1101 64-65 0.846611725940928 1101 66-67 0.8887854176928833 1101 68-69 0.8635723068281962 1101 70-71 0.8833566815910601 1101 72-73 0.9861183961477877 1101 74-75 0.9451709675097355 1101 76-77 0.9898726605898069 1102 1 0.6228617890566426 1102 2 0.838350295079529 1102 3 0.744866249857381 1102 4 0.7807629116929036 1102 5 0.7527038502028489 1102 6 0.8058824573627348 1102 7 0.6819430193819329 1102 8 0.7172873659928456 1102 9 0.6280909946581659 1102 10-11 0.6256890439127005 1102 12-13 0.8269912517942686 1102 14-15 0.6141152236027381 1102 16-17 0.6882940388577694 1102 18-19 0.8940843877713078 1102 20-21 0.9473619378524845 1102 22-23 1.0143368450469694 1102 24-25 1.0255844440723862 1102 26-27 0.806249842870038 1102 28-29 0.7643132148625185 1102 30-31 0.9619526965363434 1102 32-33 1.0135421510944198 1102 34-35 1.1471780410332997 1102 36-37 1.1146681748683562 1102 38-39 1.1747004387165347 1102 40-41 1.1532748510262323 1102 42-43 1.0534066344208846 1102 44-45 1.1033852399695405 1102 46-47 0.8539708287612129 1102 48-49 0.868578226158597 1102 50-51 0.8375980286933604 1102 52-53 0.8839815022989725 1102 54-55 0.9025190884137011 1102 56-57 1.0482360967548452 1102 58-59 1.1627793093057122 1102 60-61 1.0654938315420424 1102 62-63 0.9357818821059922 1102 64-65 0.8908719602454518 1102 66-67 0.9324889403891028 1102 68-69 0.9265554835555037 1102 70-71 1.026910955477323 1102 72-73 1.0649011403372732 1102 74-75 1.0250170359832147 1102 76-77 1.1554149981497694 1103 1 0.45698907970892577 1103 2 0.33520441440036564 1103 3 0.34648311622538586 1103 4 0.43935530038700676 1103 5 0.48139844838768653 1103 6 0.4526691348296339 1103 7 0.5380131313044885 1103 8 0.5748384637090211 1103 9 0.47848689124885624 1103 10-11 0.4633467756733012 1103 12-13 0.31471113434805886 1103 14-15 0.36729554729807745 1103 16-17 0.43077683460315086 1103 18-19 0.5041824155348067 1103 20-21 0.5923185984416719 1103 22-23 0.6017095113249482 1103 24-25 0.6011112688557176 1103 26-27 0.3486523269591615 1103 28-29 0.42517283586408894 1103 30-31 0.707235025748183 1103 32-33 0.573509806610069 1103 34-35 0.7628163461659625 1103 36-37 0.6149476809031071 1103 38-39 0.5063797830544203 1103 40-41 0.46759823810259604 1103 42-43 0.4801612281223733 1103 44-45 0.3705229729263877 1103 46-47 0.03713929996927945 1103 48-49 0.20803338493003665 1103 50-51 0.20705235163056912 1103 52-53 0.32844849694324196 1103 54-55 0.4552685357278996 1103 56-57 0.46516324805029896 1103 58-59 0.3446459750862765 1103 60-61 0.4382355135216862 1103 62-63 0.21065926113432454 1103 64-65 0.1958340039138271 1103 66-67 0.30649740966478234 1103 68-69 0.2459104904709477 1103 70-71 0.22781227617209154 1103 72-73 0.2633386670961073 1103 74-75 0.22916494643597574 1103 76-77 0.25229112521599717 1104 1 0.43187623415660426 1104 2 0.29783721616305314 1104 3 0.15088349779804133 1104 4 0.3296603502750557 1104 5 0.40809891583402447 1104 6 0.19673879365529245 1104 7 0.35765138098865634 1104 8 0.37129032387573346 1104 9 0.3328370476262208 1104 10-11 0.12146578013358322 1104 12-13 0.23078027158531 1104 14-15 0.25378734963391736 1104 16-17 0.13033720300718699 1104 18-19 0.21821066460417882 1104 20-21 0.3061698446016621 1104 22-23 0.36563225277773626 1104 24-25 0.3583993760451065 1104 26-27 0.13173563335367078 1104 28-29 0.08570133581152817 1104 30-31 0.19562708074700907 1104 32-33 0.19013055333427076 1104 34-35 0.21461044666394713 1104 36-37 -0.018245520995236575 1104 38-39 0.20318780579805917 1104 40-41 0.16162032429755868 1104 42-43 0.15420268547608273 1104 44-45 0.14345005566061886 1104 46-47 -0.1272406246284703 1104 48-49 0.05728181669417509 1104 50-51 -0.019637307234749102 1104 52-53 0.010878503944031337 1104 54-55 0.21446586967735826 1104 56-57 0.0866597555895332 1104 58-59 0.11590144631065868 1104 60-61 0.1259570150616618 1104 62-63 0.25111731689867156 1104 64-65 0.23971337221672329 1104 66-67 0.11707938233989879 1104 68-69 0.11114781319534472 1104 70-71 0.07991122327747746 1104 72-73 0.03174312717277772 1104 74-75 0.09554361565174574 1104 76-77 0.2755734532851868 1105 1 -0.22523839034932536 1105 2 0.1654261489693809 1105 3 -0.07490701603595085 1105 4 -0.030517515337592727 1105 5 -0.06917381143870216 1105 6 -0.39812286642376193 1105 7 -0.3918545478651012 1105 8 0.1815670037176318 1105 9 -0.18741986936982613 1105 10-11 -0.12902829101266633 1105 12-13 -0.06912091418544009 1105 14-15 -0.3430505950301139 1105 16-17 -0.1305323622102037 1105 18-19 0.17572054602710807 1105 20-21 0.12015205804039653 1105 22-23 -0.16574126500882613 1105 24-25 -0.25993066348058136 1105 26-27 -0.28402523621154074 1105 28-29 0.021472086799668944 1105 30-31 -0.06795987577473284 1105 32-33 0.02634596835403613 1105 34-35 0.09628040713825925 1105 36-37 0.12775250272017757 1105 38-39 -0.024578992620917006 1105 40-41 0.07639305157028531 1105 42-43 0.029449721049200406 1105 44-45 -0.08456377833147855 1105 46-47 -0.20061018984586454 1105 48-49 -0.18283676038092267 1105 50-51 -0.07200884873672564 1105 52-53 -0.10942782016664054 1105 54-55 -0.258360217279165 1105 56-57 -0.21077107444999044 1105 58-59 -0.2810847196972439 1105 60-61 -0.47680978335731083 1105 62-63 -0.4739320902159534 1105 64-65 -0.4075198293643041 1105 66-67 -0.29670520264034295 1105 68-69 -0.7369253093342998 1105 70-71 -0.8328852194102687 1105 72-73 -0.8968635921948085 1105 74-75 -0.8130532222929148 1105 76-77 -0.739495716675286 1106 1 0.14638610013980724 1106 2 0.1659800490434904 1106 3 -0.14654154234523986 1106 4 0.05011257977110262 1106 5 -0.06167589580135058 1106 6 0.10965682416418332 1106 7 0.3366070381032813 1106 8 0.1781181555209912 1106 9 0.15488651649973662 1106 10-11 0.04713123220867743 1106 12-13 0.07845099632591968 1106 14-15 0.017761264221370254 1106 16-17 0.3264824830837654 1106 18-19 0.2214144071931159 1106 20-21 0.07262858966094399 1106 22-23 -0.04272912938629503 1106 24-25 0.10992887363651249 1106 26-27 0.16376437439863167 1106 28-29 0.12577031277546524 1106 30-31 0.13516319425985301 1106 32-33 0.14080160230907524 1106 34-35 0.14310426305475943 1106 36-37 0.20247497369349787 1106 38-39 0.20384495902187183 1106 40-41 0.21203874079953522 1106 42-43 0.1880398041149114 1106 44-45 0.03884207023571662 1106 46-47 0.06201371393377997 1106 48-49 0.09600174783301085 1106 50-51 -0.0520925706364288 1106 52-53 0.08979092983335946 1106 54-55 0.2117397935286398 1106 56-57 -0.0246066105171856 1106 58-59 -0.01964870963054466 1106 60-61 0.06548251376472436 1106 62-63 -0.1977125450702033 1106 64-65 -0.14697642830205027 1106 66-67 -0.2249182090632651 1106 68-69 -0.35245110911196775 1106 70-71 -0.323777712616792 1106 72-73 -0.3550808059423396 1106 74-75 -0.4842411546051686 1106 76-77 -0.6024170281253838 1107 1 0.08533322212524297 1107 2 0.14249171556092222 1107 3 0.180474914644968 1107 4 0.20450179449173334 1107 5 0.2171382942953528 1107 6 -0.060784111992695955 1107 7 0.10377289889602537 1107 8 0.1527858257991639 1107 9 0.15644043543478858 1107 10-11 0.15928015472539414 1107 12-13 0.3623282479227399 1107 14-15 0.405456809902482 1107 16-17 0.3336241210225239 1107 18-19 0.32087081538318074 1107 20-21 0.15978982414041099 1107 22-23 0.3141620254011954 1107 24-25 -0.03585665756871492 1107 26-27 0.027104152857923225 1107 28-29 0.03375308162396351 1107 30-31 0.44751535743440485 1107 32-33 0.37375443070269654 1107 34-35 0.36561477485931704 1107 36-37 0.3538193094444466 1107 38-39 0.26356287516620824 1107 40-41 0.346334272676323 1107 42-43 0.386610543802707 1107 44-45 0.3520500080938831 1107 46-47 0.2583750625787147 1107 48-49 0.4610721965107807 1107 50-51 0.4917088691170335 1107 52-53 0.5955029343701312 1107 54-55 0.5175379351684448 1107 56-57 0.46185582680373116 1107 58-59 0.3807957802064905 1107 60-61 0.37896910305257236 1107 62-63 0.45640128275632463 1107 64-65 0.44526395717430844 1107 66-67 0.5464057373311952 1107 68-69 0.5207398718156817 1107 70-71 0.4838660575300544 1107 72-73 0.3977675013493247 1107 74-75 0.2616940322381929 1107 76-77 0.2530146460776983 1108 1 -0.2014841158012075 1108 2 6.136369414306841E-4 1108 3 -0.05195213749973249 1108 4 -0.06440857542552436 1108 5 -0.0969546733060298 1108 6 -0.08959301490416749 1108 7 -0.2855108223836069 1108 8 -0.04625530831327751 1108 9 -0.1046157658036364 1108 10-11 0.010226679306050812 1108 12-13 0.11005852061196464 1108 14-15 0.19084416596528087 1108 16-17 0.0239666459465937 1108 18-19 0.03373153032045906 1108 20-21 -0.10700038973980242 1108 22-23 0.06561078751445137 1108 24-25 0.14832661620439325 1108 26-27 -0.08107209710183838 1108 28-29 0.17111920527312208 1108 30-31 0.31928180088438296 1108 32-33 0.2796499534911945 1108 34-35 0.25858277867975943 1108 36-37 0.3041636587356322 1108 38-39 0.29554765243245384 1108 40-41 0.3300967347133934 1108 42-43 0.38597201531574754 1108 44-45 0.24639634809152255 1108 46-47 0.28580562931299625 1108 48-49 0.4140511465338541 1108 50-51 0.29371205102789943 1108 52-53 0.1478287341404787 1108 54-55 0.30276767127910986 1108 56-57 0.37072198264760914 1108 58-59 0.3242357048708584 1108 60-61 0.28849994558822445 1108 62-63 0.3636563614724011 1108 64-65 0.2382043271988863 1108 66-67 0.17321937286557088 1108 68-69 0.31358097480656966 1108 70-71 0.1324909408242192 1108 72-73 0.21214016052535456 1108 74-75 0.18494921290419697 1108 76-77 0.03443065006984014 1109 1 0.20040661681742478 1109 2 0.2293350661391642 1109 3 -0.16462707858210024 1109 4 0.3423921836590651 1109 5 0.2054428762214613 1109 6 0.2531823500988466 1109 7 0.16544684639281826 1109 8 0.35485567157151365 1109 9 0.4090923140988778 1109 10-11 0.2591297466018929 1109 12-13 0.39234153640743585 1109 14-15 0.3272302111202592 1109 16-17 0.4516300406043712 1109 18-19 0.2701044230196814 1109 20-21 0.4286777714574086 1109 22-23 0.4274182928246475 1109 24-25 0.24095703577668104 1109 26-27 0.2272732479130255 1109 28-29 0.29293432021842847 1109 30-31 0.4872783642461158 1109 32-33 0.4893666433312305 1109 34-35 0.6024372937299276 1109 36-37 0.6263327268438843 1109 38-39 0.5889352105672074 1109 40-41 0.5437517797985763 1109 42-43 0.6280657789043893 1109 44-45 0.454997204164286 1109 46-47 0.2466626460139274 1109 48-49 0.435618154269644 1109 50-51 0.38031057097400023 1109 52-53 0.5393717498285824 1109 54-55 0.5397519965939175 1109 56-57 0.5089249903547675 1109 58-59 0.4947562871220228 1109 60-61 0.3100175197743411 1109 62-63 0.38040650597046977 1109 64-65 0.3158220461514851 1109 66-67 0.5541938982587631 1109 68-69 0.5281112845066431 1109 70-71 0.5548058938243194 1109 72-73 0.6132078580940323 1109 74-75 0.5011705973656859 1109 76-77 0.5227447602391031 1110 1 -0.1095048901929232 1110 2 -0.21056910226046455 1110 3 -0.3857115344765347 1110 4 -0.1950467615058784 1110 5 -0.12314664108012607 1110 6 -0.11749934152826569 1110 7 -0.47755101045787285 1110 8 -0.08245892968584201 1110 9 0.03411594545656982 1110 10-11 -0.06265125430211782 1110 12-13 0.03462336311998371 1110 14-15 -0.0967125863856424 1110 16-17 -0.03203714652504175 1110 18-19 0.1515937012578661 1110 20-21 -0.07164973767718408 1110 22-23 -0.17927223864888475 1110 24-25 -0.05574776474028198 1110 26-27 -0.07069668956270192 1110 28-29 -0.024530735448458074 1110 30-31 0.08033166885517318 1110 32-33 0.13795095314090844 1110 34-35 0.15561515278047722 1110 36-37 0.20221151258738246 1110 38-39 0.18439977298320542 1110 40-41 0.20724726148298345 1110 42-43 0.17952649757264183 1110 44-45 0.18954884856305654 1110 46-47 0.05551149383485665 1110 48-49 0.15859483742383773 1110 50-51 0.1762245803302953 1110 52-53 0.11943908630818001 1110 54-55 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-0.6032916677677136 2314 20-21 -0.6156333815493937 2314 22-23 -0.5294822292932722 2314 24-25 -0.5400934844042737 2314 26-27 -0.6508619345699813 2314 28-29 -0.7655209338890003 2314 30-31 -0.7163325567561074 2314 32-33 -0.8147100085715024 2314 34-35 -0.8023504371447601 2314 36-37 -0.7384632683868944 2314 38-39 -0.6032498077047777 2314 40-41 -0.5854842407417884 2314 42-43 -0.5316837938886643 2314 44-45 -0.5731489544166521 2314 46-47 -0.6268711840416401 2314 48-49 -0.6410165310606928 2314 50-51 -0.5126401915419798 2314 52-53 -0.44719221499625306 2314 54-55 -0.5763751195331999 2314 56-57 -0.35531359127511664 2314 58-59 -0.3437939209934022 2314 60-61 -0.298512414842552 2314 62-63 -0.19961355231045985 2314 64-65 -0.11029374952952864 2314 66-67 -0.2898594240554324 2314 68-69 -0.036886584839052716 2314 70-71 -0.036885415258293364 2314 72-73 -0.07745772387589867 2314 74-75 0.13320875114296982 2314 76-77 0.34772119391119105 2315 1 -1.5057181743426753 2315 2 -1.2958002803752962 2315 3 -1.263091477655749 2315 4 -1.3049394206269866 2315 5 -1.2247338426564482 2315 6 -1.1197389511144777 2315 7 -1.2504998488393966 2315 8 -1.26268163050608 2315 9 -1.201266449264594 2315 10-11 -1.2898885727276266 2315 12-13 -1.4017425123325253 2315 14-15 -1.7289311745724163 2315 16-17 -1.5263722516893239 2315 18-19 -1.4380078658960542 2315 20-21 -1.538488050466448 2315 22-23 -1.6669142906124108 2315 24-25 -1.756638167068111 2315 26-27 -1.6457608420623586 2315 28-29 -1.5579773857715082 2315 30-31 -1.6415056701907886 2315 32-33 -1.707304053045224 2315 34-35 -1.5586942913839366 2315 36-37 -1.8410069381297518 2315 38-39 -1.6734580490394322 2315 40-41 -1.7919794717800812 2315 42-43 -1.758257033564231 2315 44-45 -1.7529759532515428 2315 46-47 -1.7191771947047556 2315 48-49 -1.6722559278238371 2315 50-51 -1.5570671302502817 2315 52-53 -1.636696928446895 2315 54-55 -1.771277660193494 2315 56-57 -1.7895722753261047 2315 58-59 -1.7034337290373927 2315 60-61 -1.7482412994319318 2315 62-63 -1.7056789936171768 2315 64-65 -1.8314627688673362 2315 66-67 -1.8316434280123097 2315 68-69 -1.769688992272794 2315 70-71 -2.062080733118382 2315 72-73 -3.129966044298232 2315 74-75 -3.679914422917271 2315 76-77 -3.649200281011531 2316 1 -2.9007311700973517 2316 2 -2.86759913393589 2316 3 -2.50122450940529 2316 4 -2.3509615069515206 2316 5 -3.101557625676861 2316 6 -2.460728070847644 2316 7 -2.864882582784503 2316 8 -2.845647432511342 2316 9 -2.514604069068085 2316 10-11 -2.5005438536971383 2316 12-13 -2.5206039737126673 2316 14-15 -2.6274256117292296 2316 16-17 -3.9833198675316552 2316 18-19 -3.6416089493300916 2316 20-21 -3.016329696729322 2316 22-23 -3.1223728843153182 2316 24-25 -3.0247627060094437 2316 26-27 -2.7792227934204874 2316 28-29 -3.0168514912818267 2316 30-31 -3.102055015308082 2316 32-33 -3.2233817247456216 2316 34-35 -3.3097757161377253 2316 36-37 -3.165478945231129 2316 38-39 -3.2405097923793775 2316 40-41 -3.222325168900781 2316 42-43 -3.4190342887689873 2316 44-45 -3.4190451572597773 2316 46-47 -3.1173522994027465 2316 48-49 -3.120534923211096 2316 50-51 -3.4568806975024167 2316 52-53 -4.425983701194131 2316 54-55 -3.564370050608641 2316 56-57 -3.444394697774957 2316 58-59 -3.5418800295170385 2316 60-61 -3.5451147296796783 2316 62-63 -3.5588607827687717 2316 64-65 -3.4509776059862567 2316 66-67 -3.5604341016387515 2316 68-69 -3.520966232407936 2316 70-71 -3.307767412721006 2316 72-73 -3.507858155853615 2316 74-75 -3.515483232962538 2316 76-77 -3.4793608011861643 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 48275.0 3 5517.0 4 8037.0 5 9494.0 6 10590.0 7 10807.0 8 13691.0 9 14884.0 10 19785.0 11 22253.0 12 15652.0 13 11423.0 14 10800.0 15 11080.0 16 10856.0 17 11543.0 18 12243.0 19 12670.0 20 13753.0 21 14581.0 22 16302.0 23 18283.0 24 21431.0 25 24917.0 26 29551.0 27 35226.0 28 40854.0 29 48476.0 30 57533.0 31 69613.0 32 84746.0 33 106865.0 34 144830.0 35 191489.0 36 263821.0 37 346611.0 38 300120.0 39 76091.0 40 427.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.250219176892614 26.35356165914829 30.062016205694626 21.33420295826447 2 21.85080533964505 26.64907663902981 29.939538899228264 21.560579122096872 3 22.050599816151845 26.710365248079526 29.440186989278605 21.798847946490024 4 21.81868994826312 26.473531042128602 30.029009608277903 21.678769401330378 5 21.951545112175896 26.620394215050496 29.87062898784632 21.557431684927288 6 21.900953566961242 26.4621298484381 29.913244067690137 21.723672516910526 7 21.967200392464463 26.32191139800682 29.930588853750635 21.780299355778087 8 21.75660412658304 26.715155826667807 29.4957142758154 22.03252577093376 9 21.74878114805367 26.68655202328961 29.70571088841072 21.858955940246 10-11 22.34494489259854 26.621415986274194 29.32858036422902 21.70505875689824 12-13 22.509116425086408 26.19765388904338 29.09513879970842 22.19809088616179 14-15 22.021149719207777 26.59491275994875 29.32290228024163 22.061035240601846 16-17 22.060990021448102 26.533497675628126 29.45874895236406 21.946763350559714 18-19 22.30874004241149 26.510554252732483 28.86115606768192 22.319549637174106 20-21 22.09887833235817 26.768770706870747 28.820129636589293 22.31222132418179 22-23 22.434417652779093 26.884870267411053 28.263687579256647 22.41702450055321 24-25 22.543710283326895 27.139572635200683 27.716971526875678 22.59974555459674 26-27 22.49493088962714 27.161330530019434 27.650524512596203 22.693214067757225 28-29 22.731888631838537 27.12622557935061 27.61439029296581 22.527495495845045 30-31 22.500927271862253 27.070680056333785 27.627597458235815 22.800795213568147 32-33 22.554739889532875 27.439428425595207 27.359747978830406 22.64608370604151 34-35 22.58781087173423 26.765432726063338 27.72047411403865 22.926282288163776 36-37 22.58702930889974 27.158973776247596 27.862612785831697 22.391384129020963 38-39 22.609557658169113 27.13938209152821 27.8606895079912 22.390370742311482 40-41 22.390781576085903 27.050388889556103 27.843064887601265 22.71576464675673 42-43 22.43935011146516 27.40269086964997 27.552358137141407 22.605600881743456 44-45 22.443558054078025 27.071485817625824 27.856025978885228 22.628930149410927 46-47 22.26918504210831 27.166402606675195 27.88510468348258 22.679307667733916 48-49 22.432351337173728 27.235130945525583 27.49864121741376 22.833876499886934 50-51 22.239792745347906 27.535699863327256 27.4895185219753 22.734988869349543 52-53 22.1762321669171 27.323776150105626 27.774651193307935 22.725340489669332 54-55 22.088606308350766 27.285474309030832 27.748907828889497 22.877011553728906 56-57 22.0858238181549 27.653476500071257 27.509966366472632 22.75073331530121 58-59 22.269969356887287 27.38233629078136 27.432226336354532 22.915468015976824 60-61 21.982142044589683 27.215354044348544 27.47291937634589 23.329584534715885 62-63 22.183974106061292 27.162648381490236 27.497549112459936 23.155828399988543 64-65 22.044154789387438 26.990964012663966 27.58759604114818 23.37728515680041 66-67 21.8618474608063 27.187889523059766 27.738684947050274 23.21157806908366 68-69 22.124587315779383 26.9832985627384 27.705510388437215 23.186603733045004 70-71 21.7483447381099 26.718046889170527 27.959605948891657 23.574002423827913 72-73 22.01061745139562 26.56415334284688 27.801815894835087 23.623413310922412 74-75 21.779048720578338 26.635435497071846 27.761209139970973 23.82430664237884 76-77 21.61301178931057 26.40365005413412 28.34448067767964 23.638857478875675 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 16592.0 1 9736.0 2 2638.5 3 2441.0 4 2485.0 5 2509.0 6 2579.0 7 2727.0 8 2848.5 9 2868.0 10 2930.0 11 3083.0 12 3269.5 13 3365.0 14 3529.0 15 4028.0 16 4615.0 17 4867.0 18 5217.0 19 5938.5 20 6875.0 21 8453.0 22 9466.0 23 10324.5 24 12547.0 25 15702.0 26 17493.0 27 19351.5 28 24828.5 29 30712.5 30 32978.0 31 37646.5 32 46826.0 33 55448.0 34 63002.0 35 66445.0 36 70639.0 37 78270.5 38 81371.0 39 81034.0 40 82825.5 41 85921.5 42 89957.5 43 92689.0 44 91169.0 45 90142.0 46 92291.0 47 94520.0 48 95093.0 49 94880.0 50 94459.5 51 91749.5 52 89247.0 53 87838.0 54 82657.0 55 75109.0 56 67713.0 57 64093.0 58 62315.0 59 55514.5 60 47040.0 61 43588.0 62 38961.5 63 28931.0 64 20883.0 65 18239.0 66 15817.5 67 12288.0 68 9621.5 69 6747.0 70 5431.0 71 4617.0 72 3406.0 73 2684.0 74 2359.0 75 2190.0 76 1579.0 77 894.5 78 652.0 79 539.5 80 366.5 81 244.0 82 170.5 83 159.0 84 135.5 85 90.5 86 57.5 87 46.0 88 46.0 89 49.0 90 40.0 91 28.0 92 30.0 93 29.5 94 24.0 95 22.5 96 24.0 97 20.5 98 27.0 99 71.0 100 105.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009329736919893585 2 0.011870011823825006 3 0.01339417676618386 4 0.014779781259237365 5 0.012054759089565475 6 0.009052616021282885 7 0.015611143955069465 8 0.017320056163168784 9 0.017458616612474137 10-11 0.020229825598581143 12-13 0.0159806384865504 14-15 0.008036506059710315 16-17 0.00584263227904227 18-19 0.01738933638782146 20-21 0.02450210611882944 22-23 0.021938737806680462 24-25 0.019028968371268103 26-27 0.02489469405852793 28-29 0.008059599467927874 30-31 0.007713198344664499 32-33 0.01027656665681348 34-35 0.011084835944428023 36-37 0.010692248004729529 38-39 0.013902231746970145 40-41 0.01099246231155779 42-43 0.014687407626367128 44-45 0.013163242684008277 46-47 0.024178798403783626 48-49 0.023024127992905704 50-51 0.03815031037540644 52-53 0.04082914572864322 54-55 0.028820573455512855 56-57 0.021453776234111734 58-59 0.016604160508424476 60-61 0.019698677209577298 62-63 0.027619716228199823 64-65 0.02554130948861957 66-67 0.02835870529116169 68-69 0.043184673366834174 70-71 0.05484684451670115 72-73 0.08724689624593557 74-75 0.08759329736919894 76-77 0.09117277564292048 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 2165120.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.12005348321652 #Duplication Level Percentage of deduplicated Percentage of total 1 73.20256746375574 32.29701191609669 2 12.4185698708538 10.95815933774262 3 5.212517601345759 6.899296660607472 4 2.6855044777167234 4.739384047451172 5 1.5820338909747587 3.4899709941033743 6 1.0028671729955116 2.6547931985474484 7 0.7146280321564482 2.207059889954379 8 0.4938801503597652 1.7432014918537488 9 0.37763201951602693 1.4995030408219956 >10 1.9904558612740961 16.401057390635927 >50 0.20421459444827603 6.222672206343409 >100 0.11049987347002546 8.538765295511507 >500 0.004208173757357576 1.2781168359450024 >1k 2.104086878678787E-4 0.09580306324050274 >5k 1.0520434393393934E-4 0.29854908079598524 >10k+ 1.0520434393393934E-4 0.6766555503488609 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14578 0.6733114099911321 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6432 0.297073603310671 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 4.618681643511676E-4 0.0 3 0.0 0.0 0.0 6.928022465267514E-4 0.0 4 0.0 0.0 0.0 0.0016627253916642033 0.0 5 0.0 0.0 0.0 0.00327926396689329 0.0 6 0.0 0.0 0.0 0.0058657256872598285 0.0 7 0.0 0.0 0.0 0.011916198640260124 0.0 8 0.0 0.0 0.0 0.01695056163168785 0.0 9 0.0 0.0 0.0 0.02378621046408513 0.0 10 0.0 0.0 0.0 0.030991353827963346 0.0 11 4.618681643511676E-5 0.0 0.0 0.04013634348211646 0.0 12 4.618681643511676E-5 0.0 0.0 0.05172923440733077 0.0 13 4.618681643511676E-5 0.0 0.0 0.06770987289388117 0.0 14 4.618681643511676E-5 0.0 0.0 0.08660028081584392 0.0 15 4.618681643511676E-5 0.0 0.0 0.10780002955956251 4.618681643511676E-5 16 4.618681643511676E-5 0.0 0.0 0.12909215193615134 4.618681643511676E-5 17 4.618681643511676E-5 0.0 0.0 0.15024571386343483 4.618681643511676E-5 18 4.618681643511676E-5 0.0 0.0 0.17047553946201596 4.618681643511676E-5 19 4.618681643511676E-5 0.0 0.0 0.19218334318652083 4.618681643511676E-5 20 4.618681643511676E-5 0.0 0.0 0.21753990540939994 4.618681643511676E-5 21 4.618681643511676E-5 0.0 0.0 0.24631429204847768 4.618681643511676E-5 22 4.618681643511676E-5 0.0 0.0 0.27596622819982264 4.618681643511676E-5 23 4.618681643511676E-5 0.0 0.0 0.30765038427431274 4.618681643511676E-5 24 4.618681643511676E-5 0.0 0.0 0.3426138043156961 4.618681643511676E-5 25 4.618681643511676E-5 0.0 0.0 0.3786395211350872 4.618681643511676E-5 26 4.618681643511676E-5 0.0 0.0 0.4155427874667455 4.618681643511676E-5 27 4.618681643511676E-5 0.0 0.0 0.4527231746970145 4.618681643511676E-5 28 4.618681643511676E-5 0.0 0.0 0.49050399054094 4.618681643511676E-5 29 4.618681643511676E-5 0.0 0.0 0.5265758941767662 1.3856044930535028E-4 30 4.618681643511676E-5 0.0 0.0 0.5652342595329589 1.3856044930535028E-4 31 4.618681643511676E-5 0.0 0.0 0.6113287023352054 1.3856044930535028E-4 32 4.618681643511676E-5 0.0 0.0 0.6576540792196276 1.8474726574046704E-4 33 4.618681643511676E-5 0.0 0.0 0.7082748300325156 1.8474726574046704E-4 34 4.618681643511676E-5 0.0 0.0 0.7649460537984037 1.8474726574046704E-4 35 4.618681643511676E-5 0.0 0.0 0.8242499261010937 1.8474726574046704E-4 36 4.618681643511676E-5 0.0 0.0 0.8853550842447532 1.8474726574046704E-4 37 4.618681643511676E-5 0.0 0.0 0.9513098581141 1.8474726574046704E-4 38 4.618681643511676E-5 0.0 0.0 1.0169875110848359 2.7712089861070056E-4 39 4.618681643511676E-5 0.0 0.0 1.081510493644694 2.7712089861070056E-4 40 4.618681643511676E-5 0.0 0.0 1.1475114543304759 2.7712089861070056E-4 41 4.618681643511676E-5 0.0 0.0 1.219886195684304 2.7712089861070056E-4 42 4.618681643511676E-5 0.0 0.0 1.2993737067691398 2.7712089861070056E-4 43 4.618681643511676E-5 0.0 0.0 1.390777416494236 2.7712089861070056E-4 44 4.618681643511676E-5 0.0 0.0 1.4856451374519657 2.7712089861070056E-4 45 4.618681643511676E-5 0.0 0.0 1.5805590452261307 3.233077150458173E-4 46 4.618681643511676E-5 0.0 0.0 1.685541678983151 3.233077150458173E-4 47 4.618681643511676E-5 0.0 0.0 1.7933878953591487 3.694945314809341E-4 48 4.618681643511676E-5 0.0 0.0 1.9024349689624593 4.1568134791605083E-4 49 4.618681643511676E-5 0.0 0.0 2.014253251551877 4.1568134791605083E-4 50 4.618681643511676E-5 0.0 0.0 2.1281037540644396 4.1568134791605083E-4 51 4.618681643511676E-5 0.0 0.0 2.2548865651788352 4.1568134791605083E-4 52 4.618681643511676E-5 0.0 0.0 2.3863342447531775 4.1568134791605083E-4 53 4.618681643511676E-5 0.0 0.0 2.5278506503103753 4.1568134791605083E-4 54 4.618681643511676E-5 0.0 0.0 2.6798976500147798 4.1568134791605083E-4 55 4.618681643511676E-5 0.0 0.0 2.8399811557788945 4.1568134791605083E-4 56 4.618681643511676E-5 0.0 0.0 3.0143825746378954 4.1568134791605083E-4 57 4.618681643511676E-5 0.0 0.0 3.187075081288797 5.080549807862844E-4 58 4.618681643511676E-5 0.0 0.0 3.375055424179722 5.080549807862844E-4 59 4.618681643511676E-5 0.0 0.0 3.5607726130653266 5.080549807862844E-4 60 4.618681643511676E-5 0.0 0.0 3.7478754064439848 5.080549807862844E-4 61 4.618681643511676E-5 0.0 0.0 3.9487418711203075 5.080549807862844E-4 62 4.618681643511676E-5 0.0 0.0 4.152979973396394 5.080549807862844E-4 63 4.618681643511676E-5 0.0 0.0 4.371582175583802 5.080549807862844E-4 64 4.618681643511676E-5 0.0 0.0 4.596465784806385 5.080549807862844E-4 65 4.618681643511676E-5 0.0 0.0 4.836452483003251 5.080549807862844E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGATTGC 230 0.0 23.164543 68 GTTAGCG 160 4.2366082E-8 22.18448 36 CAATTCG 350 0.0 21.300547 54 TACGTAG 410 0.0 20.775263 2 AATAGCG 120 5.130595E-5 20.70743 39 CGCGGAA 320 0.0 19.96834 59 ATTCGCG 370 0.0 19.189684 56 TAACGCG 150 1.3235347E-5 18.936008 52 AATTCGC 375 0.0 18.933819 55 ATACGTA 435 0.0 18.766262 1 TTAGTCG 420 0.0 18.593998 15 TGTTTCG 325 0.0 18.567991 42 CGGTTAC 365 0.0 18.475231 4 CGATCGT 390 0.0 18.201391 1 AACTCGA 395 0.0 17.973068 13 ATTGCCG 120 0.0012208193 17.747995 25 ACGCGAA 205 5.7541E-7 17.317518 54 AACGCGA 165 3.0066763E-5 17.21336 53 TAGTCGG 455 0.0 17.16369 16 TCGCGCT 445 0.0 16.751692 37 >>END_MODULE