FastQCFastQC Report
Thu 26 May 2016
SRR936143_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936143_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2165120
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122990.5680516553355011No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92780.4285212828850133No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65580.3028931421814957No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA13750.051.726547100-101
ACGTATC4750.043.22886100-101
CGTACAT16050.042.459873100-101
TACGCAG10000.042.257774100-101
GGTATCA102300.041.5341761
CGGTATC12200.040.9796100-101
TCGAGTA4450.040.124493100-101
TCGCGAC300.001307785739.6736296-97
CGAGTAC11500.038.298534100-101
AGGTATC25050.036.471104100-101
CCGTACA6900.035.365765100-101
AGTATCA37300.034.147053100-101
TCGTATC6400.033.943855100-101
GCGTATC3450.032.778023100-101
GCGTACA4000.032.734898100-101
CCGTATC5300.031.443468100-101
GTATCAA200600.031.32681
AAGTATC23700.031.265781100-101
GGGTATC15300.031.120522100-101
AACGTAT3550.031.01641798-99
Produced by FastQC (version 0.11.5)