FastQCFastQC Report
Thu 26 May 2016
SRR936142_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936142_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2172139
Sequences flagged as poor quality0
Sequence length77
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147460.6788699986510992No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA64840.2985076001121475No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGCG800.00246014222.18258728
ACGCCGT900.00486375319.7214859
TATAGCG1851.960234E-719.1880343
GATTCGT3001.8189894E-1218.93001233
CGCGGAA3750.017.9864159
TCATACG1200.00122057917.74852214
TTGCGCG4250.017.53815
ATTTGCG4400.016.940213
TAAGGCG6100.016.87486517
AGTCACG6100.016.8740864
TACGTAG3501.4551915E-1116.2257232
TAGCGAC2201.2026558E-616.13836345
TATCGGC1553.622019E-416.04385271
TGCGCGC4900.015.9374486
ATTCGTC3703.45608E-1115.34865934
TCGCGGA4450.015.15673958
ATTCGCG3754.3655746E-1115.14610256
TCGCGCT4500.014.98763937
CGATCGT4301.8189894E-1214.8578581
TCGTCAG4600.014.6618236
Produced by FastQC (version 0.11.5)