FastQCFastQC Report
Thu 26 May 2016
SRR936142_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936142_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2172139
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123280.567551155796199No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90440.4163637778245315No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66080.3042162587200911No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA15150.051.268013100-101
CGTACAT16900.045.430923100-101
CGGTATC14550.044.587395100-101
GCGTACA4750.041.97588100-101
GGTATCA104950.040.3163071
CCGGTAT5300.039.304367100-101
ACGTATC4400.038.551456100-101
TCGTATC5100.037.92816100-101
TACGCAG9350.037.23856100-101
GCGTATC3800.035.24094100-101
CGAGTAC11200.034.010307100-101
AGGTATC26950.033.89988100-101
AGTATCA36700.033.813377100-101
CCGTACA8100.033.800365100-101
CCGTATC7450.033.154343100-101
CCTGTCG6050.032.95285496-97
TCGGTAT4700.032.924873100-101
GGGTATC14800.032.775135100-101
AGCGTAC3000.032.7326698-99
ACCGTAC3100.032.6366798-99
Produced by FastQC (version 0.11.5)