FastQCFastQC Report
Thu 26 May 2016
SRR936141_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936141_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2604689
Sequences flagged as poor quality0
Sequence length77
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54510.2092764241719453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAC2650.028.12514
TTAGTCG2700.021.0338415
CGAGCGT850.003498636720.87637330
TAGTCGG3001.8189894E-1218.93154716
CGATCGT3051.8189894E-1218.6186891
GGTTCGC1602.3099828E-517.75002922
CGCGGAA3206.366463E-1116.63969259
CGAACTC5400.015.77598525
CAATTCG3401.5825208E-1015.66359554
GTTAGCG1604.620505E-415.53873566
TCGCGCT3201.1350494E-915.52948537
TCGAACT6050.015.25622424
TGGCGTA2604.144149E-715.01954318
GGGCGGT5200.015.0157941
CTCGCGC3352.1736923E-914.83242736
CGCTCCG4101.0913936E-1114.7178425
CGTATGA1450.00432332714.68573132
GGCGGTT5650.014.4477442
ATCGTTC3951.0004442E-1014.3759043
TTAGCGC2001.5485282E-414.20793667
Produced by FastQC (version 0.11.5)