FastQCFastQC Report
Thu 26 May 2016
SRR936141_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936141_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2604689
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78480.30130276589642757No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57430.22048697560438116No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38170.1465434069096157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA12000.047.622498100-101
GGTATCA83850.036.0501981
CGTACAT16600.035.68101100-101
CGGTATC9550.035.529873100-101
ACGTATC3700.031.372929100-101
GTATCAA163400.030.5896341
CCGTATC6350.029.998423100-101
TCGTATC5900.029.764061100-101
CCGGTAT3850.029.377516100-101
GCGTATC3150.029.291615100-101
CCTGTCG6750.028.6564696-97
CCGTACA8150.028.48585100-101
CGAGTAC9150.026.999094100-101
GCGTACA5850.026.965729100-101
TACCGTA2900.026.68553298-99
TACGCAG10000.026.490013100-101
AGTATCA32200.026.343966100-101
AGGTATC25650.026.340902100-101
CCGTACG1158.267307E-925.8770796-97
AACGTAT3400.025.3874898-99
Produced by FastQC (version 0.11.5)